FprEN 17710
(Main)Plant biostimulants - Detection of Listeria monocytogenes
Plant biostimulants - Detection of Listeria monocytogenes
This document specifies a method for the detection of Listeria monocytogenes in microbial plant biostimulants.
This document is applicable to the blends of fertilizing products where a blend is a mix of at least two of the following component EU fertilizing products categories: Fertilizers, Liming Materials, Soil Improvers, Growing Media, Plant Biostimulants and where the following category Plant Biostimulants is the highest percentage in the blend by mass or volume, or in the case of liquid form by dry mass. If Plant Biostimulants is not the highest percentage in the blend, the European Standard for the highest percentage of the blend applies. In case a blend of fertilizing products is composed of components in equal quantity or in case the component EU fertilising products used for the blend have identical formulations , the user decides which standard to apply.
Pflanzen-Biostimulanzien - Nachweis von Listeria monocytogenes
Dieses Dokument legt ein Verfahren zum Nachweis von Listeria monocytogenes in mikrobiellen Pflanzen-Biostimulanzien fest.
Dieses Dokument ist für die Mischungen von Düngeprodukten anwendbar, in denen eine Mischung aus mindestens zwei der folgenden Kategorien von Komponenten-EU Düngeprodukten besteht: Düngemittel, Kalkdünger, Bodenverbesserungsmittel, Kultursubstrate, Pflanzen-Biostimulanzien und wenn die folgende Kategorie Pflanzen-Biostimulanzien prozentual den höchsten Massen- oder Volumenanteil in der Mischung bzw., bei flüssiger Form, den höchsten Trockenmasseanteil aufweist. Wenn Pflanzen-Biostimulanzien nicht den höchsten prozentualen Anteil in der Mischung ausmachen, ist die für den höchsten prozentualen Anteil geltende Europäische Norm anwendbar. Falls eine Düngemittelmischung aus Komponenten gleicher Menge besteht oder falls die für die Mischung verwendeten Komponenten-EU Düngeprodukte identische Formulierungen haben, entscheidet der Anwender, welche Norm angewendet wird.
Biostimulants des végétaux - Détection de Listeria monocytogenes
Le présent document spécifie une méthode pour la recherche de Listeria monocytogenes dans les biostimulants microbiens des végétaux.
Le présent document est applicable aux mélanges de fertilisants constitués d’au moins deux composants appartenant aux catégories de fertilisants UE suivantes : engrais, amendements minéraux basiques, amendements du sol, supports de culture, biostimulants des végétaux et, dans lesquels la catégorie Biostimulants des végétaux représente le pourcentage le plus élevé du mélange en masse ou en volume, ou dans le cas d’un liquide, en masse sèche. Si les biostimulants des végétaux ne représentent pas le pourcentage le plus élevé du mélange, la Norme européenne relative au composant représentant le pourcentage le plus élevé du mélange s’applique. Dans le cas où un mélange de fertilisants contient des composants en quantité égale ou dans le cas où les composants des fertilisants UE utilisés pour le mélange ont des formulations identiques, l’utilisateur décide quelle norme appliquer.
Rastlinski biostimulanti - Ugotavljanje prisotnosti Listeria monocytogenes
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
oSIST prEN 17710:2023
01-maj-2023
Rastlinski biostimulanti - Ugotavljanje prisotnosti Listeria monocytogenes
Plant biostimulants - Detection of Listeria monocytogenes
Pflanzen-Biostimulanzien - Nachweis von Listeria monocytogenes
Biostimulants des végétaux - Détection de Listeria monocytogenes
Ta slovenski standard je istoveten z: prEN 17710
ICS:
65.080 Gnojila Fertilizers
oSIST prEN 17710:2023 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
oSIST prEN 17710:2023
oSIST prEN 17710:2023
DRAFT
EUROPEAN STANDARD
prEN 17710
NORME EUROPÉENNE
EUROPÄISCHE NORM
April 2023
ICS 65.080 Will supersede CEN/TS 17710:2022
English Version
Plant biostimulants - Detection of Listeria monocytogenes
Biostimulants des végétaux - Détection de Listeria Pflanzen-Biostimulanzien - Nachweis von Listeria
monocytogenes monocytogenes
This draft European Standard is submitted to CEN members for enquiry. It has been drawn up by the Technical Committee
CEN/TC 455.
If this draft becomes a European Standard, CEN members are bound to comply with the CEN/CENELEC Internal Regulations
which stipulate the conditions for giving this European Standard the status of a national standard without any alteration.
This draft European Standard was established by CEN in three official versions (English, French, German). A version in any other
language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC
Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of which they are
aware and to provide supporting documentation.
Warning : This document is not a European Standard. It is distributed for review and comments. It is subject to change without
notice and shall not be referred to as a European Standard.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATIO N
EUROPÄISCHES KOMITEE FÜR NORMUN G
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2023 CEN All rights of exploitation in any form and by any means reserved Ref. No. prEN 17710:2023 E
worldwide for CEN national Members.
oSIST prEN 17710:2023
prEN 17710:2023 (E)
Contents Page
European foreword . 5
Introduction . 6
1 Scope . 8
2 Normative references . 8
3 Terms and definitions . 8
4 Principle . 9
4.1 General . 9
4.2 Pre-enrichment in selective liquid medium . 9
4.3 Enrichment in/on selective media . 9
4.4 Plating out on selective solid media . 9
4.5 Confirmation . 9
5 Culture media, reagents, antisera . 9
6 Equipment and consumables . 10
7 Sampling . 10
8 Preparation of test sample . 10
9 Preparation of test sample . 10
9.1 Test portion and initial suspension . 10
9.1.1 General . 10
9.1.2 Liquid - water based- formulations . 11
9.1.3 Liquid - oil based (emulsifiable concentrate - EC) formulations . 11
9.1.4 Solid - Wettable Powder (WP) formulations . 11
9.1.5 Solid - Water dispersible granules (WDG) formulations . 11
9.1.6 Solid – Pellets, granules, microgranules (slow release) formulations . 11
9.1.7 Solid substrates . 11
9.2 Non-selective pre-enrichment . 12
9.3 Selective enrichment. 12
9.4 Plating out . 12
9.4.1 General . 12
9.4.2 Agar Listeria according to Ottaviani and Agosti (B.3) . 12
9.4.3 Second selective medium . 13
9.5 Confirmation of L. monocytogenes . 13
9.5.1 General . 13
9.5.2 Selection of colonies for confirmation . 13
9.5.3 Confirmation tests for L. monocytogenes . 13
9.6 Interpretation of morphological and physiological properties and of the biochemical
reactions . 16
9.7 Additional characterization of isolated strains (optional) . 16
10 Expression of results . 16
11 Performance characteristics of the method . 16
11.1 Interlaboratory studies . 16
11.2 Sensitivity . 16
11.3 Specificity . 16
oSIST prEN 17710:2023
prEN 17710:2023 (E)
11.4 Predictive positive value (PPV) . 16
11.5 Predictive negative value (NPV) . 17
12 Test report . 17
13 Quality assurance . 17
Annex A (normative) Diagram of the procedures . 18
Annex B (normative) Composition and preparation of culture media and reagents . 19
B.1 Selective primary enrichment medium: half-Fraser broth . 19
B.1.1 Base . 19
B.1.2 Lithium chloride solution . 19
B.1.3 Solution of sodium salt of nalidixic acid . 20
B.1.4 Acriflavine hydrochloride solution . 20
B.1.5 Ammonium iron(III) citrate solution . 20
B.1.6 Complete medium . 21
B.2 Selective secondary enrichment medium: Fraser broth . 21
B.2.1 Base . 21
B.2.2 Acriflavine hydrochloride solution . 21
B.2.3 Ammonium iron(III) citrate solution . 22
B.2.4 Complete medium . 22
B.3 Agar Listeria according to Ottaviani and Agosti (ALOA) . 22
B.3.1 Base medium . 22
B.3.2 Nalidixic acid solution . 22
B.3.3 Ceftazidime solution . 23
B.3.4 Polymyxin B solution . 23
B.3.5 Antibiotic supplement . 23
B.3.6 Supplement . 24
B.3.7 Complete medium . 24
B.4 Second selective solid plating-out medium . 25
B.5 Performance testing for the quality assurance of the culture media . 25
B.6 Hydrogen peroxide solution . 25
B.7 Motility agar . 25
B.7.1 Composition . 25
B.7.2 Preparation . 25
B.8 Blood agar . 25
B.8.1 Base . 25
B.8.2 Defibrinated blood (sheep, calf or bovine) . 26
B.8.3 Complete medium . 26
B.9 Phosphate-
...
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