EN 26461-2:1993

Water quality - Detection and enumeration of the spores of sulfite-reducing anaerobes (clostridia) - Part 2: Method by membrane filtration (ISO 6461-2:1986)

The procedure is applicable to all types of water, except when a large amount of particulate material is liable to be retained by the membrane. The principle covers several steps from selection by applying heat to destroy vegetative bacteria to filtration of the water sample through a membrane filter having a suitable pore size (0,2 µm) to retain the spores in or on it. The filter is placed on a selective culture medium, followed by incubation and counting of black colonies.

Wasserbeschaffenheit - Nachweis und Zählung der Sporen sulfitreduzierender Anaerobier (Clostridien) - Teil 2: Membranfiltrationsverfahren (ISO 6461-2:1986)

Qualité de l'eau - Recherche et dénombrement des spores de micro-organismes anaérobies sulfito-réducteurs (clostridia) - Partie 2: Méthode par filtration sur membrane (ISO 6461-2:1986)

La présente partie de l'ISO 6461 spécifie une méthode de recherche et de dénombrement des spores de microorganismes anaérobies sulfito-réducteurs (clostridia) par filtration sur membrane.
Le principe de la méthode est applicable à tous les types d'eau, sauf dans le cas où une grande quantité de matières sous forme de particules est susceptible d'être retenue par la membrane.

Kakovost vode - Ugotovljanje prisotnosti in števila spor sulfit reducirajočih anaerobov (klostridiji) - 2. del: Metoda membranske filtracije (ISO 6461-2:1986)

General Information

• Status: Published
• Publication Date: 24-Jan-1993
• Withdrawal Date: 30-Jul-1993
• ICS: 13.060.30 - Sewage water
• Technical Committee: CEN/TC 230 - Water analysis
• Drafting Committee: CEN/TC 230 - Water analysis
• Current Stage: 9093 - Decision to confirm - Review Enquiry
• Start Date: 20-Jan-2000
• Completion Date: 20-Jan-2000
• Ref Project: SIST EN 26461-2:1998 - Water quality - Detection and enumeration of the spores of sulfite-reducing anaerobes (clostridia) - Part 2: Method by membrane filtration (ISO 6461-2:1986)

Standards Content (Sample)

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.HGXFLUDMRþLKWasserbeschaffenheit - Nachweis und Zählung der Sporen sulfitreduzierender Anaerobier (Clostridien) - Teil 2: Membranfiltrationsverfahren (ISO 6461-2:1986)Qualité de l'eau - Recherche et dénombrement des spores de micro-organismes anaérobies sulfito-réducteurs (clostridia) - Partie 2: Méthode par filtration sur membrane (ISO 6461-2:1986)Water quality - Detection and enumeration of the spores of sulfite-reducing anaerobes (clostridia) - Part 2: Method by membrane filtration (ISO 6461-2:1986)13.060.70Preiskava bioloških lastnosti vodeExamination of biological properties of waterICS:Ta slovenski standard je istoveten z:EN 26461-2:1993SIST EN 26461-2:1998en01-maj-1998SIST EN 26461-2:1998SLOVENSKI
STANDARD



SIST EN 26461-2:1998



SIST EN 26461-2:1998



International Standard 646112 INTERNATIONAL ORGANIZATION FOR STANDARDIZATlON.MEX~YHAPO~HAR OPrAHkl3At.&lR l-l0 CTAHAAPTM3AlJklt4~0RGANISATION INTERNATIONALE DE NORMALISATION Water quality - Detection and enumeration of the spores of sulfite-reducing anaerobes (clostridia) - Part 2: Method by membrane filtration Qua&@ de l’eau - Recherche et dhombrement des spores de micro-organismes anaerobies sulfito-rgducteurs (clostridia) _ Partie 2 : tI& thode par filtra tion sur membrane First edition - 1986-02-01 UDC 543.39 : 579.852.13 Ref. No. ISO 6461/2-1986 (E) Descriptors : water, quality, tests, microbiological analysis, determination, micro-organisms, sulphite reducing bacteria. Price based on 3 pages SIST EN 26461-2:1998



Foreword ISO (the International Organkation for Standardization) is a worldwide federation of national Standards bodies (ISO member bedies). The work of preparing International Standards is normally carried out through ISO technical committees. Esch member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, govern- mental and non-governmental, in liaison with ISO, also take part in the work. Draft International Standards adopted by the technical committees are circulated to the member bodies for approval before their acceptance as International Standards by the ISO Council. They are approved in accordance with ISO procedures requiring at least 75 % approval by the member bodies voting. International Standard ISO 6461/2 was prepared by Technical Committee ISO/TC 147, Water quality. Users should note that all International Standards undergo revision from time to time and that any reference made herein to any other International Standard implies its latest edition, unless otherwise stated. 0 International Organkation for Standardkation, 1986 Printed in Switzerland SIST EN 26461-2:1998



INTERNATIONAL STANDARD ISO 6461/2-1986 (EI Water quality - Detection and enumeration of the spores of sulfite-reducing anaerobes (clostridia) - Part 2: Method by membrane filtration 0 Introduction The spores of sulfite-reducing anaerobes (clostridia) are wide- spread in the environment. They are present in human and animal faecal matter, in waste water and in soil. Unlike Escherichia col; and other coliform organisms, the spores sur- vive in water for long periods as they are more resistant than vegetative forms to the action of Chemical and physical factors. They may thus give an indication of remote OF intermittent pollution. They may even be resistant to chlorination at levels which are normally used for the treatment sf water, and they are thus useful for control purposes. ISO 6461 consists of the following Parts: Part 1 : Method by enrichment in a liquid medium. Part 2: Method by membrane filtration. 1 Scope This part of ISO 6461 specifies a method for the detection and enumeration of the spores of sulfite-reducing anaerobes (clostridia) by membrane filtration. 2 Field of application The method tan be applied to all types of water, except when a large amount of particulate material is liable to be retained by the membrane. 3 References ISO 3696, Water for laboratory use - Specifications. ISO 5667, Water quality - Sampling - Part 2: Guidance on sampling techniques. Part 3: Guidance on the preservation and handling of samples. IS 0 7704, Water quality - Evaluation of membrane filters used for microbiological analyses. ISO 8199, Water quality - General guidance for microbiological examina tion by enumeration of micro- organisms on culture media. ’ ) 4 Definition For the purpose of this part of ISO 6461, the following defi- nition applies. clostridia : Sulfite-reducing, Spore-forming, anaerobic micro- organisms which belong to the Bacillaceae family and the genus Clostridium. 5 Principle The detection of spores of sulfite-reducing (clostridia) in a specified volume of a water Sample following Steps. 5.1 Selection of spores Selection of spores in the Sample by applying heat of time sufficient to destroy vegetative ba’cteria. 5.2 Membrane
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