Soil quality - Assessment of genotoxic effects on higher plants - Vicia faba micronucleus test (ISO 29200:2013)

The purpose of ISO 29200:2013 is to describe a method for assessing genotoxic effects (chromosome breakage or dysfunction of the mitotic spindle) of soils or soil materials on the secondary roots of a higher plant: Vicia faba (broad bean). This method allows the assessment of genotoxicity (toxicity for genetic material) of soils and soil materials like compost, sludge, waste, fertilizing matters, etc. Two ways of exposure can be considered: a direct exposure of plants to the soil (or soil material) which is relevant for the real genotoxic potential and an exposure of plants to the water extract of the soil (or soil material). This last way of exposure to a leachate or an eluate allows the detection of the mutagens which are not adsorbed to soils and which may be transferred to aquatic compartments. Moreover, this test may be used to evaluate genotoxic effects of chemical substances and to waters, effluents, etc.

Bodenbeschaffenheit - Beurteilung der genotoxischen Wirkungen auf höhere Pflanzen - Mikrokern-Prüfung mit Vicia faba (ISO 29200:2013)

Zweck dieser Internationalen Norm ist es, ein Verfahren zur Beurteilung der genotoxischen Wirkungen (Chromosomenbruch oder Dysfunktion der Mitosespindel) von Böden oder Bodenmaterialien auf die Seitenwurzeln einer höheren Pflanze, Vicia faba (Ackerbohne), zu beschreiben. Dieses Verfahren ermöglicht die Beurteilung der Genotoxizität (Toxizität für genetisches Material) von Boden und Boden¬material sowie Kompost, Schlamm, Abfall, Dünger, usw. Zwei Wege der Exposition können in Betracht gezogen werden: die direkte Exposition der Pflanzen mit Boden (oder Bodenmaterial), welche für das echte genotoxische Potenzial wichtig ist, und die Exposition der Pflanzen gegenüber dem Wasserextrakt des Bodens (oder Boden¬materials). Der letztere Weg der Exposition der Pflanzen gegenüber Sickerwasser oder Eluat erlaubt die Erfassung der Mutagene, die nicht an Böden adsorbieren und in die Bodenlösung gelangen können. Darüber hinaus darf die Prüfung zur Abschätzung genotoxischer Wirkungen von chemischen Substanzen und Wässern, Abwasser usw. verwendet werden.

Qualité du sol - Évaluation des effets génotoxiques sur les végétaux supérieurs - Essai des micronoyaux sur Vicia faba (ISO 29200:2013)

L'ISO 29200:2013 décrit une méthode pour évaluer les effets génotoxiques (cassure des chromosomes ou dysfonctionnement du fuseau mitotique) des sols ou des matrices solides sur une plante supérieure: Vicia faba (fève). Cette méthode permet d'évaluer la génotoxicité (toxicité vis-à-vis du matériel génétique) des sols ou des matrices solides telles que des composts, boues, déchets, matières fertilisantes, etc. Deux modes d'exposition peuvent être considérés: une exposition directe des plantes au sol (ou aux matrices solides) ce qui est représentatif du potentiel génotoxique réel et une exposition des plantes à l'extrait aqueux du sol (ou des matrices solides). Ce dernier mode d'exposition à un lixiviat ou un éluat permet de détecter les mutagènes qui ne sont pas adsorbés dans les sols et qui peuvent être transférés aux compartiments aquatiques. D'autre part, cet essai peut être utilisé pour évaluer les effets génotoxiques des substances chimiques, ainsi que des eaux, effluents, etc.

Kakovost tal - Ocenjevanje genotoksičnih učinkov na višje rastline - Mikronukleusni preskus z bobom (Vicia faba) (ISO 29200:2013)

General Information

Status
Published
Publication Date
05-May-2020
Withdrawal Date
29-Nov-2020
Current Stage
6060 - Definitive text made available (DAV) - Publishing
Start Date
06-May-2020
Due Date
07-Mar-2022
Completion Date
06-May-2020

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Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN ISO 29200:2020
01-november-2020
Kakovost tal - Ocenjevanje genotoksičnih učinkov na višje rastline -
Mikronukleusni preskus z bobom (Vicia faba) (ISO 29200:2013)
Soil quality - Assessment of genotoxic effects on higher plants - Vicia faba micronucleus
test (ISO 29200:2013)
Bodenbeschaffenheit - Beurteilung der genotoxischen Wirkungen auf höhere Pflanzen -
Mikrokern-Prüfung mit Vicia faba (ISO 29200:2013)
Qualité du sol - Évaluation des effets génotoxiques sur les végétaux supérieurs - Essai
des micronoyaux sur Vicia faba (ISO 29200:2013)
Ta slovenski standard je istoveten z: EN ISO 29200:2020
ICS:
13.080.30 Biološke lastnosti tal Biological properties of soils
SIST EN ISO 29200:2020 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN ISO 29200:2020

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SIST EN ISO 29200:2020


EN ISO 29200
EUROPEAN STANDARD

NORME EUROPÉENNE

May 2020
EUROPÄISCHE NORM
ICS 13.080.30
English Version

Soil quality - Assessment of genotoxic effects on higher
plants - Vicia faba micronucleus test (ISO 29200:2013)
Qualité du sol - Évaluation des effets génotoxiques sur Bodenbeschaffenheit - Beurteilung der genotoxischen
les végétaux supérieurs - Essai des micronoyaux sur Wirkungen auf höhere Pflanzen - Mikrokern-Prüfung
Vicia faba (ISO 29200:2013) mit Vicia faba (ISO 29200:2013)
This European Standard was approved by CEN on 13 April 2020.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2020 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 29200:2020 E
worldwide for CEN national Members.

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SIST EN ISO 29200:2020
EN ISO 29200:2020 (E)
Contents Page
European foreword . 3

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SIST EN ISO 29200:2020
EN ISO 29200:2020 (E)
European foreword
The text of ISO 29200:2013 has been prepared by Technical Committee ISO/TC 190 "Soil Quality” of the
International Organization for Standardization (ISO) and has been taken over as EN ISO 29200:2020 by
Technical Committee CEN/TC 444 “Environmental characterization of solid matrices” the secretariat of
which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by November 2020, and conflicting national standards
shall be withdrawn at the latest by November 2020.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.
Endorsement notice
The text of ISO 29200:2013 has been approved by CEN as EN ISO 29200:2020 without any modification.

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SIST EN ISO 29200:2020

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SIST EN ISO 29200:2020
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