Soil quality - Assessment of genotoxic effects on higher plants - Vicia faba micronucleus test (ISO 29200:2013)

The purpose of ISO 29200:2013 is to describe a method for assessing genotoxic effects (chromosome breakage or dysfunction of the mitotic spindle) of soils or soil materials on the secondary roots of a higher plant: Vicia faba (broad bean). This method allows the assessment of genotoxicity (toxicity for genetic material) of soils and soil materials like compost, sludge, waste, fertilizing matters, etc. Two ways of exposure can be considered: a direct exposure of plants to the soil (or soil material) which is relevant for the real genotoxic potential and an exposure of plants to the water extract of the soil (or soil material). This last way of exposure to a leachate or an eluate allows the detection of the mutagens which are not adsorbed to soils and which may be transferred to aquatic compartments. Moreover, this test may be used to evaluate genotoxic effects of chemical substances and to waters, effluents, etc.

Bodenbeschaffenheit - Beurteilung der genotoxischen Wirkungen auf höhere Pflanzen - Mikrokern-Prüfung mit Vicia faba (ISO 29200:2013)

Zweck dieser Internationalen Norm ist es, ein Verfahren zur Beurteilung der genotoxischen Wirkungen (Chromosomenbruch oder Dysfunktion der Mitosespindel) von Böden oder Bodenmaterialien auf die Seitenwurzeln einer höheren Pflanze, Vicia faba (Ackerbohne), zu beschreiben. Dieses Verfahren ermöglicht die Beurteilung der Genotoxizität (Toxizität für genetisches Material) von Boden und Boden¬material sowie Kompost, Schlamm, Abfall, Dünger, usw. Zwei Wege der Exposition können in Betracht gezogen werden: die direkte Exposition der Pflanzen mit Boden (oder Bodenmaterial), welche für das echte genotoxische Potenzial wichtig ist, und die Exposition der Pflanzen gegenüber dem Wasserextrakt des Bodens (oder Boden¬materials). Der letztere Weg der Exposition der Pflanzen gegenüber Sickerwasser oder Eluat erlaubt die Erfassung der Mutagene, die nicht an Böden adsorbieren und in die Bodenlösung gelangen können. Darüber hinaus darf die Prüfung zur Abschätzung genotoxischer Wirkungen von chemischen Substanzen und Wässern, Abwasser usw. verwendet werden.

Qualité du sol - Évaluation des effets génotoxiques sur les végétaux supérieurs - Essai des micronoyaux sur Vicia faba (ISO 29200:2013)

L'ISO 29200:2013 décrit une méthode pour évaluer les effets génotoxiques (cassure des chromosomes ou dysfonctionnement du fuseau mitotique) des sols ou des matrices solides sur une plante supérieure: Vicia faba (fève). Cette méthode permet d'évaluer la génotoxicité (toxicité vis-à-vis du matériel génétique) des sols ou des matrices solides telles que des composts, boues, déchets, matières fertilisantes, etc. Deux modes d'exposition peuvent être considérés: une exposition directe des plantes au sol (ou aux matrices solides) ce qui est représentatif du potentiel génotoxique réel et une exposition des plantes à l'extrait aqueux du sol (ou des matrices solides). Ce dernier mode d'exposition à un lixiviat ou un éluat permet de détecter les mutagènes qui ne sont pas adsorbés dans les sols et qui peuvent être transférés aux compartiments aquatiques. D'autre part, cet essai peut être utilisé pour évaluer les effets génotoxiques des substances chimiques, ainsi que des eaux, effluents, etc.

Kakovost tal - Ocenjevanje genotoksičnih učinkov na višje rastline - Mikronukleusni preskus z bobom (Vicia faba) (ISO 29200:2013)

General Information

Status
Published
Publication Date
05-May-2020
Withdrawal Date
29-Nov-2020
Current Stage
6060 - Definitive text made available (DAV) - Publishing
Start Date
06-May-2020
Due Date
07-Mar-2022
Completion Date
06-May-2020

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SLOVENSKI STANDARD
01-november-2020
Kakovost tal - Ocenjevanje genotoksičnih učinkov na višje rastline -
Mikronukleusni preskus z bobom (Vicia faba) (ISO 29200:2013)
Soil quality - Assessment of genotoxic effects on higher plants - Vicia faba micronucleus
test (ISO 29200:2013)
Bodenbeschaffenheit - Beurteilung der genotoxischen Wirkungen auf höhere Pflanzen -
Mikrokern-Prüfung mit Vicia faba (ISO 29200:2013)
Qualité du sol - Évaluation des effets génotoxiques sur les végétaux supérieurs - Essai
des micronoyaux sur Vicia faba (ISO 29200:2013)
Ta slovenski standard je istoveten z: EN ISO 29200:2020
ICS:
13.080.30 Biološke lastnosti tal Biological properties of soils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 29200
EUROPEAN STANDARD
NORME EUROPÉENNE
May 2020
EUROPÄISCHE NORM
ICS 13.080.30
English Version
Soil quality - Assessment of genotoxic effects on higher
plants - Vicia faba micronucleus test (ISO 29200:2013)
Qualité du sol - Évaluation des effets génotoxiques sur Bodenbeschaffenheit - Beurteilung der genotoxischen
les végétaux supérieurs - Essai des micronoyaux sur Wirkungen auf höhere Pflanzen - Mikrokern-Prüfung
Vicia faba (ISO 29200:2013) mit Vicia faba (ISO 29200:2013)
This European Standard was approved by CEN on 13 April 2020.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2020 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 29200:2020 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
The text of ISO 29200:2013 has been prepared by Technical Committee ISO/TC 190 "Soil Quality” of the
International Organization for Standardization (ISO) and has been taken over as EN ISO 29200:2020 by
Technical Committee CEN/TC 444 “Environmental characterization of solid matrices” the secretariat of
which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by November 2020, and conflicting national standards
shall be withdrawn at the latest by November 2020.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.
Endorsement notice
The text of ISO 29200:2013 has been approved by CEN as EN ISO 29200:2020 without any modification.

INTERNATIONAL ISO
STANDARD 29200
First edition
2013-09-01
Soil quality — Assessment of
genotoxic effects on higher plants —
Vicia faba micronucleus test
Qualité du sol — Évaluation des effets génotoxiques sur les végétaux
supérieurs — Essai des micronoyaux sur Vicia faba
Reference number
ISO 29200:2013(E)
©
ISO 2013
ISO 29200:2013(E)
© ISO 2013
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior
written permission. Permission can be requested from either ISO at the address below or ISO’s member body in the country of
the requester.
ISO copyright office
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Tel. + 41 22 749 01 11
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Published in Switzerland
ii © ISO 2013 – All rights reserved

ISO 29200:2013(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative reference . 1
3 Terms and definitions . 1
4 Principle . 2
5 Plants, test equipment and reagents . 2
5.1 Equipment . 2
5.2 Test organism . 2
5.3 Reference substance . 2
5.4 Reagents. 3
6 Protocols . 3
6.1 Preparation of the soil to be tested . 3
6.2 Preparation of the seeds . 4
6.3 Conducting of the test . 5
6.4 Test environment . 6
6.5 Cell preparation . 6
7 Assessment of the results . 8
7.1 Presentation of the data . 8
7.2 Statistical analysis . 8
7.3 Interpretation of the results . 8
8 Validity criteria . 8
9 Test report . 8
Annex A (informative) Composition of Hoagland’s medium .10
Annex B (informative) Testing chemicals added to soils .11
Annex C (informative) Results of the interlaboratory test conducted within the framework of
NF T 90-327
.............................................................................................................................................................................................................12
Annex D (informative) Results of the interlaboratory test conducted on the reference substance
and an industrial contaminated soil .14
Bibliography .18
ISO 29200:2013(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2. www.iso.org/directives
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of any
patent rights identified during the development of the document will be in the Introduction and/or on
the ISO list of patent declarations received. www.iso.org/patents
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
The committee responsible for this document is ISO/TC 190, Soil quality, Subcommittee SC 4,
Biological methods.
iv © ISO 2013 – All rights reserved

ISO 29200:2013(E)
Introduction
In the field of assessment of the quality of soils and soil materials, it appears necessary to determine
in vivo their genotoxic potential which may be induced by pollution or by a decontamination process.
Indeed, genotoxic agents have the ability to damage the genome of living organisms or to interfere with
its functioning, but they are not always detected by chemical analysis or classical ecotoxicological tests.
Actually, genotoxic effects are often observed at sublethal concentrations, where no toxic effect (e.g.
survival or growth) can be observed in the short term but some long term effects may be feared in living
organisms. Moreover, higher plants, like Vicia faba (broad bean) are ecologically relevant to assess soils
and soil materials quality.
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