EN ISO 23611-1:2011
(Main)Soil quality - Sampling of soil invertebrates - Part 1: Hand-sorting and formalin extraction of earthworms (ISO 23611-1:2006)
Soil quality - Sampling of soil invertebrates - Part 1: Hand-sorting and formalin extraction of earthworms (ISO 23611-1:2006)
ISO 23611-1:2006 specifies a method for sampling and handling earthworms from field soils as a prerequisite for using these animals as bioindicators (e.g. to assess the quality of a soil as a habitat for organisms).
ISO 23611-1:2006 applies to all terrestrial biotopes in which earthworms occur. It is not applicable for semi-terrestrial soils and it can be difficult to use under extreme climatic or geographical conditions (e.g. in high mountains).
Bodenbeschaffenheit - Probenahme von Wirbellosen im Boden - Teil 1: Handauslese und Formalinextraktion von Regenwürmern (ISO 23611-1:2006)
Dieser Teil von ISO 23611 legt ein Verfahren zur Probenahme und Behandlung von Regenwürmern aus Freilandböden fest, mit dessen Hilfe die Voraussetzungen für die Verwendung dieser Tiere als Bioindikatoren (z. B. zur Beurteilung der Beschaffenheit eines Bodens als Lebensraum für Organismen) geschaffen werden.
Grundlegende Informationen zur Ökologie von Regenwürmern und ihrer Verwendung als Bioindikatoren in der terrestrischen Umwelt lassen sich den im Literaturverzeichnis aufgeführten Quellen entnehmen.
Dieser Teil von ISO 23611 gilt für alle terrestrischen Biotope, in denen Regenwürmer leben. Der für Freilanduntersuchungen im Allgemeinen gültige Probenahmeplan ist bereits in ISO 10381-1 (siehe auch [38]) festgelegt, und eine Anleitung zur Bestimmung der Wirkungen von Schadstoffen auf Regenwürmer unter Freilandbedingungen wird in ISO 11268-3 gegeben. Diese Einzelheiten können den nationalen Anforderun¬gen entsprechend oder in Abhängigkeit von den klimatischen/regionalen Bedingungen des zu beprobenden Standorts variieren (siehe auch Anhang C).
Dieser Teil von ISO 23611 gilt nicht für semiterrestrische Böden. Die Anwendung unter extremen klimatischen oder geographischen Bedingungen (z. B. im Hochgebirge) kann schwierig sein. Die Verfahren für einige andere Bodenorganismengruppen wie z. B. Collembolen werden in anderen Teilen von ISO 23611 behandelt.
Dieser Teil von ISO 23611 behandelt nicht die Charakterisierung des Standortes nach bodenkundlichen Gesichtspunkten, wie sie für die Probenahme von Wirbellosen im Boden nachdrücklich empfohlen wird. Vor allem sollten der pH-Wert, die Korngrößenverteilung, das C/N-Verhältnis, der Gehalt an organischem Kohlenstoff und das Wasserrückhaltevermögen nach ISO 10390, ISO 10694, ISO 11272, ISO 11274, ISO 11277, ISO 11461 und ISO 11465 bestimmt werden.
Qualité du sol - Prélèvement des invertébrés du sol - Partie 1 : Tri manuel et extraction au formol des vers de terre (ISO 23611-1:2006)
L'ISO 23611-1:2006 spécifie une méthode pour le prélèvement et la manipulation de vers de terre sur le terrain, comme prérequis à l'utilisation de ces animaux en tant que bio-indicateurs, par exemple pour évaluer la qualité d'un sol en tant qu'habitat pour des organismes.
L'ISO 23611-1:2006 est utilisée pour tous les biotopes terrestres dans lesquels il est possible de trouver des vers de terre. Elle ne s'applique pas aux sols semi-terrestres et il peut être difficile de l'appliquer dans des conditions climatiques ou géographiques extrêmes (en haute montagne par exemple).
Kakovost tal - Vzorčenje nevretenčarjev v tleh - 1. del: Ročno razvrščanje deževnikov in njihova ekstrakcija s formalinom (ISO 23611-1:2006)
ISO 23611-1:2006 določa metodo za vzorčenje in delo z deževniki iz poljskih tal kot nujen pogoj za uporabo teh živali kot bioindikatorjev (npr. za ocenjevanje kakovosti tal kot habitata za organizme).
ISO 23611-1:2006 velja za vse zemeljske biotope, v katerih so deževniki. Ne velja za polzemeljska tla in se lahko s težavo uporablja v ekstremnih klimatskih ali geografskih razmerah (npr. v visokih gorah.
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Standards Content (Sample)
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.]YUãþDQMHBodenbeschaffenheit - Probenahme von Wirbellosen im Boden - Teil 1: Handauslese und Formalinextraktion von Regenwürmern (ISO 23611-1:2006)Qualité du sol - Prélèvement des invertébrés du sol - Partie 1 : Tri manuel et extraction au formol des vers de terre (ISO 23611-1:2006)Soil quality - Sampling of soil invertebrates - Part 1: Hand-sorting and formalin extraction of earthworms (ISO 23611-1:2006)13.080.30Biološke lastnosti talBiological properties of soilsICS:Ta slovenski standard je istoveten z:EN ISO 23611-1:2011SIST EN ISO 23611-1:2011en,fr,de01-december-2011SIST EN ISO 23611-1:2011SLOVENSKI
STANDARD
EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN ISO 23611-1
July 2011 ICS 13.080.30; 13.080.05 English Version
Soil quality - Sampling of soil invertebrates - Part 1: Hand-sorting and formalin extraction of earthworms (ISO 23611-1:2006)
Qualité du sol - Prélèvement des invertébrés du sol -Partie 1 : Tri manuel et extraction au formol des vers de terre (ISO 23611-1:2006)
Bodenbeschaffenheit - Probenahme von Wirbellosen im Boden - Teil 1: Handauslese und Formalinextraktion von Regenwürmern (ISO 23611-1:2006) This European Standard was approved by CEN on 17 June 2011.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
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Avenue Marnix 17,
B-1000 Brussels © 2011 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 23611-1:2011: ESIST EN ISO 23611-1:2011
Reference numberISO 23611-1:2006(E)© ISO 2006
INTERNATIONAL STANDARD ISO23611-1First edition2006-02-01Soil quality — Sampling of soil invertebrates — Part 1: Hand-sorting and formalin extraction of earthworms Qualité du sol — Prélèvement des invertébrés du sol — Partie 1: Tri manuel et extraction au formol des vers de terre
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ii © ISO 2006 – All rights reserved
ISO 23611-1:2006(E) © ISO 2006 – All rights reserved iiiContents Page Foreword.iv Introduction.v 1 Scope.1 2 Terms and definitions.1 3 Principle.2 4 Reagents.2 5 Apparatus.2 6 Procedure.3 6.1 Sampling of the earthworms.3 6.2 Preservation.5 6.3 Determination of biomass.5 7 Data assessment.5 8 Study report.6 Annex A (informative)
Other methods for sampling.7 Annex B (informative)
Species determination in peregrine earthworms.8 Annex C (informative)
The modified TSBF Method.9 Bibliography.10
ISO 23611-1:2006(E) iv © ISO 2006 – All rights reserved Foreword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. ISO 23611-1 was prepared by Technical Committee ISO/TC 190, Soil quality, Subcommittee SC 4, Biological methods. ISO 23611 consists of the following parts, under the general title Soil quality — Sampling of soil invertebrates: ⎯ Part 1: Hand-sorting and formalin extraction of earthworms ⎯ Part 2: Sampling and extraction of micro-arthropods (Collembola and Acarina) ⎯ Part 3: Sampling and soil extraction of enchytraeids ⎯ Part 4: Sampling, extraction and identification of free-living stages of terrestrial nematodes SIST EN ISO 23611-1:2011
ISO 23611-1:2006(E) © ISO 2006 – All rights reserved vIntroduction This part of ISO 23611 has been drawn up since there is a growing need for the standardisation of terrestrial zoological field methods. Such methods, mainly covering the sampling, extraction and handling of soil invertebrates, are necessary for the following purposes: ⎯ biological classification of soils including soil quality assessment [21], [26], [34]; ⎯ terrestrial bioindication and long-term monitoring [9], [12], [28]; ⎯ evaluation of the effects of chemicals on soil animals (ISO 11268-3). Data for these purposes are gained by standardized methods since they can form the basis for far-reaching decisions (e.g. whether a given site should be remediated or not). In fact, the lack of such standardised methods is one of the most important reasons why bio-classification and bio-assessment in terrestrial (i.e. soil) habitats has so far relatively rarely been used in comparison to aquatic sites. Originally, the methods described here were developed for taxonomical and ecological studies, investigating the role of earthworms in various soil ecosystems. These animals are without doubt the most important soil invertebrates in temperate regions and, to a lesser extent, in tropical soils [25], [13], [15]. Since Darwin (1881), their influence on soil structure (e.g. aeration, water holding capacity) and soil functions like litter decomposition and nutrient cycling is well-known [8]. Due to their often very high biomass they are also important in many terrestrial food-webs. Since it is neither possible nor useful to standardize methods for all soil organisms, the most important ones have been selected. SIST EN ISO 23611-1:2011
INTERNATIONAL STANDARD ISO 23611-1:2006(E) © ISO 2006 – All rights reserved 1Soil quality — Sampling of soil invertebrates — Part 1: Hand-sorting and formalin extraction of earthworms 1 Scope This part of ISO 23611 specifies a method for sampling and handling earthworms from field soils as a prerequisite for using these animals as bioindicators (e.g. to assess the quality of a soil as a habitat for organisms). Basic information on the ecology of earthworms and their use as bioindicators in the terrestrial environment can be found in the references listed in the bibliography. This part of ISO 23611 applies to all terrestrial biotopes in which earthworms occur. The sampling design of field studies in general is specified in ISO 10381-1 (see also Reference [38]) and guidance on the determination of effects of pollutants on earthworms in field situations is given in ISO 11268-3. These details can vary according to the national requirements or the climatic/regional conditions of the site to be sampled (see also Annex C). This part of ISO 23611 is not applicable for semi-terrestrial soils and it can be difficult to use under extreme climatic or geographical conditions (e.g. in high mountains). Methods for some other soil organism groups, such as collembolans, are covered in other parts of ISO 23611. This part of ISO 23611 does not cover the pedological characterization of the site which is highly recommendable when sampling soil invertebrates. ISO 10390, ISO 10694, ISO 11272, ISO 11274, ISO 11277, ISO 11461 and ISO 11465 are more suitable for measuring pH, particle size distribution, C/N ratio, organic carbon content and water-holding capacity. 2 Terms and definitions For the purposes of this document, the following terms and definitions apply. 2.1 earthworm megadrile soil-inhabiting earthworms (length of adult individuals: few centimetres to more than 1 m) belonging to the order Oligochaeta (class Clitellata, phylum Annelida) EXAMPLE Species of the families Lumbricidae (Holarctic), Glossoscolecidae (Latin America), Eudrilidae (Africa) or Megascolecidae [Asia, North America (Pacific Coast)]. 2.2 peregrine species earthworms occurring in many regions world-wide today, usually introduced by man NOTE 1 Well-known examples of peregrine species are several lumbricid species like Aporrectodea caliginosa (originally coming from Eurasia, but now living also in the Americas and Australia) or the pan-tropical species Pontoscolex corethrurus (probably coming from Northern Brazil and/or the Guyanas). NOTE
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