Animal feeding stuffs - Isolation and enumeration of yeast probiotic strains

This European Standard defines general rules for the enumeration of probiotic yeasts in feed samples (additives, premixtures and feeding stuffs) that contain yeast as a single microorganism component or in a mixture with other microorganisms. The standard is not applicable to mineral feeds which are defined as complementary feedingstuffs composed mainly of minerals and containing at least 40% crude ash (Council Directive 79/373/EEC) [4].
There are different categories of feed samples:
a)   Additives which contain about 109 CFU/g to 1010 CFU/g (CFU = colony forming units).
b)   Premixtures which contain about 108 CFU/g
c)   Feeds, meal or pellets, which contain about 106 CFU/g and include complete feedingstuffs, and milk replacers.
The detection limit is as defined in ISO 7218.

Futtermittel - Keimzählung von Hefestämmen

Diese Europäische Norm legt allgemeine Regeln für die Zählung von probiotischen Hefen in Futtermittel-proben (Zusatzstoffe, Vormischungen und Futtermittel) fest, die Hefen als einzelnen mikrobiellen Bestandteil oder in einem Gemisch mit anderen probiotischen Mikroorganismen enthalten. Diese Norm ist nicht anwendbar auf mineralische Futtermittel, die als Ergänzungsfuttermittel definiert sind, hauptsächlich aus Mineralien zusammengesetzt sind und mindestens 40 % Rohasche enthalten (Richtlinie 79/373/EWG) [4].
Es gibt unterschiedliche Kategorien von Futtermittelproben:
a)   Zusatzstoffe, die etwa 109 KbE/g bis 1010 KbE/g (KbE/g  koloniebildende Einheiten) enthalten;
b)   Vormischungen, die etwa 108 KbE/g enthalten;
c)   Futtermittel, Mehl oder Pellets, die etwa 106 KbE/g enthalten, einschließlich Fertigfuttermittel und Milch-austauscher.
Die Nachweisgrenze entspricht der in EN ISO 7218 festgelegten.

Aliments des animaux - Isolation et dénombrement de souches probiotiques de levures (saccharomyces cerevisiae)

La présente Norme internationale donne des directives générales pour le dénombrement des levures probiotiques présentes dans des échantillons d’aliments (additifs, prémélanges et aliments des animaux) qui contiennent de la levure en tant que composant sous forme d’un micro-organisme simple ou en mélange avec d’autres micro-organismes. La présente norme ne s’applique pas aux aliments minéraux qui sont définis comme étant des aliments complémentaires des animaux constitués principalement de minéraux et renfermant au moins 40 % de cendres brutes (Directive du Conseil 79/373/CEE).
Il existe différentes catégories d’échantillons d’aliments des animaux :
   les additifs qui contiennent environ 109 UFC/g à 1010 UFC/g ;
   les prémélanges qui contiennent environ 108 UFC/g ;
   les aliments des animaux, les farines ou les granulés qui contiennent environ 106 UFC/g et incluent des aliments complets et des succédanés de lait.

Krma - Izolacija in štetje domnevno prisotnih kvasovk probiotičnih sevov

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2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Futtermittel - Keimzählung von probiotischen HefestämmenAliments des animaux - Isolation et dénombrement de souches probiotiques de levures (saccharomyces cerevisiae)Animal feeding stuffs - Isolation and enumeration of yeast probiotic strains65.120KrmilaAnimal feeding stuffsICS:Ta slovenski standard je istoveten z:EN 15789:2009SIST EN 15789:2009en,fr,de01-december-2009SIST EN 15789:2009SLOVENSKI

SIST EN 15789:2009

EN 15789
September 2009 ICS 65.120 English Version
Animal feeding stuffs - Isolation and enumeration of yeast probiotic strains
Aliments des animaux - Isolation et dénombrement de souches probiotiques de levures (saccharomyces cerevisiae)
Futtermittel - Keimzählung von Hefestämmen This European Standard was approved by CEN on 1 August 2009.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
Management Centre:
Avenue Marnix 17,
B-1000 Brussels © 2009 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 15789:2009: ESIST EN 15789:2009

EN 15789:2009 (E) 2 Contents Page Foreword .3 Introduction .4 1 Scope .5 2 Normative references .5 3 Terms and definitions .5 4 Principle .5 5 Diluent, selective media and test kit for phenotypic characterisation .6 6 Apparatus and glassware .8 7 Sampling .9 8 Preparation of test samples .9 9 Procedure .9 10 Expression of results . 12 11 Precision . 13 12 Test report . 13 Annex A (informative) Results of the interlaboratory study . 14 Bibliography . 15
SIST EN 15789:2009

EN 15789:2009 (E) 3 Foreword This document (EN 15789:2009) has been prepared by Technical Committee CEN/TC 327 “Animal feeding stuffs”, the secretariat of which is held by NEN. This European Standard shall be given the status of a national standard, eith

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