prEN ISO 13136-2

Microbiology of the food chain - Detection, isolation and characterization of Shiga toxin-producing Escherichia coli (STEC) - Part 2: Horizontal method for the characterization of Shiga toxin-producing Escherichia coli (STEC) isolates (ISO/DIS 13136‑2:2024)

This document is applicable to pure cultures of STEC. The present standard describes the characterization of STEC strains, isolated from any source. In particular, the characterization of STEC strains described here regard:
-   subtyping of the Stx-coding genes;
-   determination of the presence of eae gene;
-   determination of the presence of aggR gene;
-   identification of the presence of genes associated to the following serogroups: O157, O26, O111, O103, O145, O121 and O45.
The full characterization of the isolated STEC strains is achieved by performing all the modules described here. The characterization scheme is not sequential, and the different modules can be implemented separately based on specific needs (e.g. Regulatory needs, Competent Authority’s requests, clients’ request). Alternative methods may be used, including Whole Genome Sequencing (WGS), provided these are verified according to the reference standard ISO 16140-2.

Mikrobiologie der Lebensmittelkette - Nachweis, Isolierung und Charakterisierung von Shiga-Toxin bildenden Escherichia coli (STEC) - Teil 2: Horizontales Verfahren zur Charakterisierung von Shiga-Toxin bildenden Escherichia coli (STEC) Isolaten (ISO/DIS 13136‑2:2024)

Microbiologie de la chaîne alimentaire - Détection, isolement et caractérisation des Escherichia coli producteurs de Shigatoxines (STEC) - Partie 2: Méthode horizontale pour la caractérisation d’isolats des Escherichia coli producteurs de Shigatoxines (STEC) (STEC) (ISO/DIS 13136‑2:2024)

Mikrobiologija v prehranski verigi - Odkrivanje prisotnosti, izolacija in karakterizacija Escherichia coli (STEC), ki proizvaja Shiga toksin - 2. del: Horizontalna metoda za karakterizacijo izolatov Escherichia coli (STEC), ki proizvaja Shiga toksin (ISO/DIS 13136-1:2024)

General Information

• Status: Not Published
• Publication Date: 10-Jun-2025
• ICS: 07.100.30 - Food microbiology
• Technical Committee: CEN/TC 463 - Microbiology of the food chain
• Drafting Committee: CEN/TC 463/WG 2 - Shiga toxin producing Escherichia coli (STEC)
• Current Stage: 4020 - Submission to enquiry - Enquiry
• Start Date: 29-Feb-2024
• Due Date: 10-Jun-2023
• Completion Date: 29-Feb-2024
• Ref Project: oSIST prEN ISO 13136-2:2024 - Microbiology of the food chain - Detection, isolation and characterization of Shiga toxin-producing Escherichia coli (STEC) - Part 2: Horizontal method for the characterization of Shiga toxin-producing Escherichia coli (STEC) isolates (ISO/DIS 13136-2:2024)

Relations

• Revises: CEN ISO/TS 13136:2012 - Microbiology of food and animal feed - Real-time polymerase chain reaction (PCR)-based method for the detection of food-borne pathogens - Horizontal method for the detection of Shiga toxin-producing Escherichia coli (STEC) and the determination of O157, O111, O26, O103 and O145 serogroups (ISO/TS 13136:2012)
• Effective Date: 17-Aug-2022

Standards Content (Sample)

SLOVENSKI STANDARD
oSIST prEN ISO 13136-2:2024
01-april-2024
Mikrobiologija v prehranski verigi - Odkrivanje prisotnosti, izolacija in
karakterizacija Escherichia coli (STEC), ki proizvaja Shiga toksin - 2. del:
Horizontalna metoda za karakterizacijo izolatov Escherichia coli (STEC), ki
proizvaja Shiga toksin (ISO/DIS 13136-1:2024)
Microbiology of the food chain - Detection, isolation and characterization of Shiga toxin-
producing Escherichia coli (STEC) - Part 2: Horizontal method for the characterization of
Shiga toxin-producing Escherichia coli (STEC) isolates (ISO/DIS 13136-2:2024)
Mikrobiologie der Lebensmittelkette - Nachweis, Isolierung und Charakterisierung von
Shiga-Toxin bildenden Escherichia coli (STEC) - Teil 2: Horizontales Verfahren zur
Charakterisierung von Shiga-Toxin bildenden Escherichia coli (STEC) Isolaten (ISO/DIS
13136-2:2024)
Microbiologie de la chaîne alimentaire - Détection, isolement et caractérisation des
Escherichia coli producteurs de Shigatoxines (STEC) - Partie 2: Méthode horizontale
pour la caractérisation d’isolats des Escherichia coli producteurs de Shigatoxines
(STEC) (STEC) (ISO/DIS 13136-2:2024)
Ta slovenski standard je istoveten z: prEN ISO 13136-2
ICS:
07.100.30 Mikrobiologija živil Food microbiology
oSIST prEN ISO 13136-2:2024 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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oSIST prEN ISO 13136-2:2024

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oSIST prEN ISO 13136-2:2024
DRAFT
International
Standard
ISO/DIS 13136-2
ISO/TC 34/SC 9
Microbiology of the food
Secretariat: AFNOR
chain — Detection, isolation and
Voting begins on:
characterization of Shiga toxin-
2024-02-28
producing Escherichia coli (STEC) —
Voting terminates on:
2024-05-22
Part 2:
Horizontal method for the
characterization of Shiga toxin-
producing Escherichia coli (STEC)
isolates
ICS: 07.100.30
THIS DOCUMENT IS A DRAFT CIRCULATED
FOR COMMENTS AND APPROVAL. IT
IS THEREFORE SUBJECT TO CHANGE
AND MAY NOT BE REFERRED TO AS AN
INTERNATIONAL STANDARD UNTIL
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This document is circulated as received from the committee secretariat.
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Reference number
ISO/DIS 13136-2:2024(en)
© ISO 2024

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oSIST prEN ISO 13136-2:2024
DRAFT
ISO/DIS 13136-2:2024(en)
International
Standard
ISO/DIS 13136-2
ISO/TC 34/SC 9
Microbiology of the food
Secretariat: AFNOR
chain — Detection, isolation and
Voting begins on:
characterization of Shiga toxin-
producing Escherichia coli (STEC) —
Voting terminates on:
Part 2:
Horizontal method for the
characterization of Shiga toxin-
producing Escherichia coli (STEC)
isolates
ICS: 07.100.30
THIS DOCUMENT IS A DRAFT CIRCULATED
FOR COMMENTS AND APPROVAL. IT
IS THEREFORE SUBJECT TO CHANGE
AND MAY NOT BE REFERRED TO AS AN
INTERNATIONAL STANDARD UNTIL
PUBLISHED AS SUCH.
This document is circulated as received from the committee secretariat.
COPYRIGHT PROTECTED DOCUMENT
IN ADDITION TO THEIR EVALUATION AS
BEING ACCEPTABLE FOR INDUSTRIAL,
© ISO 2024
TECHNOLOGICAL, COMMERCIAL AND
USER PURPOSES, DRAFT INTERNATIONAL
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
STANDARDS MAY ON OCCASION HAVE TO
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NATIONAL REGULATIONS.
ISO copyright office
RECIPIENTS OF THIS DRAFT ARE INVITED
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TO SUBMIT, WITH THEIR COMMENTS,
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NOTIFICATION OF ANY RELEVANT PATENT
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Published in Switzerland Reference number
ISO/DIS 13136-2:2024(en)
© ISO 2024
© ISO 2024 – All rights reserved
ii

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oSIST prEN ISO 13136-2:2024
ISO/DIS 13136-2:2024(en)
Contents Page
Foreword .
...