ISO 19007:2018

Nanotechnologies — In vitro MTS assay for measuring the cytotoxic effect of nanoparticles

ISO 19007:2018 specifies a method for evaluating the effects of nano-objects and their aggregates and agglomerates (NOAA) on cellular viability using the MTS assay. The assay design includes performance requirements and control experiments to identify and manage variability in the assay results. ISO 19007:2018 is applicable to the use of a 96-well plate.

Nanotechnologies - Analyse du MTS in vitro pour la mesure de l'effet cytotoxique des nanoparticules

General Information

• Status: Published
• Publication Date: 09-Apr-2018
• ICS: 07.030 - Physics. Chemistry
• ICS: 07.120 - Nanotechnologies
• Technical Committee: ISO/TC 229 - Nanotechnologies
• Drafting Committee: ISO/TC 229 - Nanotechnologies
• Current Stage: 9093 - International Standard confirmed
• Completion Date: 04-Oct-2023

Standards Content (Sample)

INTERNATIONAL ISO
STANDARD 19007
First edition
2018-04
Nanotechnologies — In vitro MTS
assay for measuring the cytotoxic
effect of nanoparticles
Nanotechnologies - Analyse du MTS in vitro pour la mesure de l'effet
cytotoxique des nanoparticules
Reference number
ISO 19007:2018(E)
©
ISO 2018

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ISO 19007:2018(E)

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© ISO 2018
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Published in Switzerland
ii © ISO 2018 – All rights reserved

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ISO 19007:2018(E)

Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Symbols and abbreviated terms . 2
5 Materials . 3
5.1 Cell line . 3
5.2 Assay . 3
5.3 Controls . 3
6 Apparatus . 4
7 Nanoparticle test sample preparation . 4
8 Preparations . 5
8.1 General . 5
8.2 Culture medium . 5
8.3 Preparation of cell stock culture . 5
8.4 Verify viable cell growth . 6
8.5 Verification of plate reader uniformity . 6
8.
...