Microscopes -- Definition and measurement of illumination properties

This document specifies measurands and measurement procedures of colour properties for bright field microscopy with transmitted light illumination. These measurements are defined in image planes or intermediate image planes. This document also specifies how the information is provided to the user.

Microscopes -- Définition et mesurage des propriétés d'éclairage

General Information

Status
Published
Publication Date
01-Dec-2019
Current Stage
6060 - International Standard published
Start Date
04-Nov-2019
Completion Date
02-Dec-2019
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ISO 19056-2:2019 - Microscopes -- Definition and measurement of illumination properties
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INTERNATIONAL ISO
STANDARD 19056-2
First edition
2019-11
Microscopes — Definition and
measurement of illumination
properties —
Part 2:
Illumination properties related to the
colour in bright field microscopy
Microscopes — Définition et mesurage des propriétés d'éclairage —
Partie 2: Propriétés d'illumination liées à la couleur en microscopie à
champ lumineux
Reference number
ISO 19056-2:2019(E)
ISO 2019
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ISO 19056-2:2019(E)
COPYRIGHT PROTECTED DOCUMENT
© ISO 2019

All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may

be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting

on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address

below or ISO’s member body in the country of the requester.
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Published in Switzerland
ii © ISO 2019 – All rights reserved
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ISO 19056-2:2019(E)
Contents  Page

Foreword ........................................................................................................................................................................................................................................iv

1 Scope ................................................................................................................................................................................................................................. 1

2  Normative references ...................................................................................................................................................................................... 1

3  Terms and definitions ..................................................................................................................................................................................... 1

4  Measurands ................................................................................................................................................................................................................ 1

4.1 General ........................................................................................................................................................................................................... 1

4.2 Spectral measurement...................................................................................................................................................................... 1

4.3 Chromaticity .............................................................................................................................................................................................. 2

4.4 Correlated colour temperature, T .......................................................................................................................................

cp 3

4.4.1 General...................................................................................................................................................................................... 3

4.4.2 Correlated colour temperature using the CIE 1960 UCS chromaticity

diagram and its procedure ...................................................................................................................................... 3

4.4.3 Correlated colour temperature using (x, y) chromaticity coordinates ............................. 3

5  Measurement procedures ........................................................................................................................................................................... 3

5.1 General ........................................................................................................................................................................................................... 3

5.2 Measurement environment.......................................................................................................................................................... 4

5.3 Integrating sphere ................................................................................................................................................................................ 4

5.4 Microscope settings ............................................................................................................................................................................ 4

5.4.1 Diaphragm settings ........................................................................................................................................... ............. 4

5.4.2 Adjustment of samples and focusing for diaphragms ..................................................................... 4

5.4.3 Other combinations ....................................................................................................................................................... 4

5.4.4 Adjustment of light source ...................................................................................................................................... 4

5.5 Stability of measurement ............................................................................................................................................................... 4

6  Information provided to the user ....................................................................................................................................................... 5

6.1 General ........................................................................................................................................................................................................... 5

6.2 Information of chromaticity and correlated colour temperature ............................................................... 5

6.3 Additional information .................................................................................................................................................................... 6

Bibliography ................................................................................................................................................................................................................................ 8

© ISO 2019 – All rights reserved iii
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ISO 19056-2:2019(E)
Foreword

ISO (the International Organization for Standardization) is a worldwide federation of national standards

bodies (ISO member bodies). The work of preparing International Standards is normally carried out

through ISO technical committees. Each member body interested in a subject for which a technical

committee has been established has the right to be represented on that committee. International

organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.

ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of

electrotechnical standardization.

The procedures used to develop this document and those intended for its further maintenance are

described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the

different types of ISO documents should be noted. This document was drafted in accordance with the

editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).

Attention is drawn to the possibility that some of the elements of this document may be the subject of

patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of

any patent rights identified during the development of the document will be in the Introduction and/or

on the ISO list of patent declarations received (see www .iso .org/ patents).

Any trade name used in this document is information given for the convenience of users and does not

constitute an endorsement.

For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and

expressions related to conformity assessment, as well as information about ISO's adherence to the

World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see www .iso .org/

iso/ foreword .html.

This document was prepared by Technical Committee ISO/TC 172, Optics and photonics, Subcommittee

SC 5, Microscopes and endoscopes.
A list of all parts in the ISO 19056 series can be found on the ISO website.

Any feedback or questions on this document should be directed to the user’s national standards body. A

complete listing of these bodies can be found at www .iso .org/ members .html.
iv © ISO 2019 – All rights reserved
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INTERNATIONAL STANDARD ISO 19056-2:2019(E)
Microscopes — Definition and measurement of
illumination properties —
Part 2:
Illumination properties related to the colour in bright field
microscopy
1 Scope

This document specifies measurands and measurement procedures of colour properties for bright field

microscopy with transmitted light illumination. These measurements are defined in image planes or

intermediate image planes.
This document also specifies how the information is provided to the user.
2  Normative references

The following documents are referred to in the text in such a way that some or all of their content

constitutes requirements of this document. For dated references, only the edition cited applies. For

undated references, the latest edition of the referenced document (including any amendments) applies.

ISO 11664-1:2019, Colorimetry — Part 1: CIE standard colorimetric observers
ISO 11664-3, Colorimetry — Part 3: CIE tristimulus values
3  Terms and definitions
No terms and definitions are listed in this document.

ISO and IEC maintain terminological databases for use in standardization at the following addresses:

— ISO Online browsing platform: available at http:// www .iso .org/ obp
— IEC Electropedia: available at http:// www .electropedia .org/
4  Measurands
4.1  General

Since various light sources such as halogen lamps, tungsten lamps, LEDs, and OLEDs are used for

bright field microscopy, it is essential to understand the difference in colour properties due to the light

sources. This applies for different applications and various types of instruments.

4.2  Spectral measurement

The spectral radiant flux (W) shall be measured by placing an instrument equipped with an integrating

sphere and an optical spectrometer function in an image plane or in an intermediate image plane. The

measurements shall be performed in the measurement spectral range from 380 nm to 780 nm with

5 nm intervals or less.
© ISO 2019 – All rights reserved 1
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ISO 19056-2:2019(E)
4.3  Chromaticity
The chromaticity coordinates (x, y) shall be calculated from the
...

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