Microscopes — Definition and measurement of illumination properties — Part 2: Illumination properties related to the colour in bright field microscopy

This document specifies measurands and measurement procedures of colour properties for bright field microscopy with transmitted light illumination. These measurements are defined in image planes or intermediate image planes. This document also specifies how the information is provided to the user.

Microscopes — Définition et mesurage des propriétés d'éclairage — Partie 2: Propriétés d'illumination liées à la couleur en microscopie à champ lumineux

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ISO 19056-2:2019 - Microscopes -- Definition and measurement of illumination properties
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STANDARD 19056-2
First edition
Microscopes — Definition and
measurement of illumination
properties —
Part 2:
Illumination properties related to the
colour in bright field microscopy
Microscopes — Définition et mesurage des propriétés d'éclairage —
Partie 2: Propriétés d'illumination liées à la couleur en microscopie à
champ lumineux
Reference number
ISO 19056-2:2019(E)
ISO 2019

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ISO 19056-2:2019(E)

© ISO 2019
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
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ii © ISO 2019 – All rights reserved

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ISO 19056-2:2019(E)

Contents  Page
Foreword .iv
1 Scope . 1
2  Normative references . 1
3  Terms and definitions . 1
4  Measurands . 1
4.1 General . 1
4.2 Spectral measurement. 1
4.3 Chromaticity . 2
4.4 Correlated colour temperature, T .
cp 3
4.4.1 General. 3
4.4.2 Correlated colour temperature using the CIE 1960 UCS chromaticity
diagram and its procedure . 3
4.4.3 Correlated colour temperature using (x, y) chromaticity coordinates . 3
5  Measurement procedures . 3
5.1 General . 3
5.2 Measurement environment. 4
5.3 Integrating sphere . 4
5.4 Microscope settings . 4
5.4.1 Diaphragm settings . . 4
5.4.2 Adjustment of samples and focusing for diaphragms . 4
5.4.3 Other combinations . 4
5.4.4 Adjustment of light source . 4
5.5 Stability of measurement . 4
6  Information provided to the user . 5
6.1 General . 5
6.2 Information of chromaticity and correlated colour temperature . 5
6.3 Additional information . 6
Bibliography . 8
© ISO 2019 – All rights reserved iii

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ISO 19056-2:2019(E)

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This document was prepared by Technical Committee ISO/TC 172, Optics and photonics, Subcommittee
SC 5, Microscopes and endoscopes.
A list of all parts in the ISO 19056 series can be found on the ISO website.
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iv © ISO 2019 – All rights reserved

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Microscopes — Definition and measurement of
illumination properties —
Part 2:
Illumination properties related to the colour in bright field
1 Scope
This document specifies measurands and measurement procedures of colour properties for bright field
microscopy with transmitted light illumination. These measurements are defined in image planes or
intermediate image planes.
This document also specifies how the information is provided to the user.
2  Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 11664-1:2019, Colorimetry — Part 1: CIE standard colorimetric observers
ISO 11664-3, Colorimetry — Part 3: CIE tristimulus values
3  Terms and definitions
No terms and definitions are listed in this document.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at http:// www .iso .org/ obp
— IEC Electropedia: available at http:// www .electropedia .org/
4  Measurands
4.1  General
Since various light sources such as halogen lamps, tungsten lamps, LEDs, and OLEDs are used for
bright field microscopy, it is essential to understand the difference in colour properties due to the light
sources. This applies for different applications and various types of instruments.
4.2  Spectral measurement
The spectral radiant flux (W) shall be measured by placing an instrument equipped with an integrating
sphere and an optical spectrometer function in an image plane or in an intermediate image plane. The
measurements shall be performed in the measurement spectral range from 380 nm to 780 nm with
5 nm intervals or less.
© ISO 2019 – All rights reserved 1

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ISO 19056-2:2019(E)

4.3  Chromaticity
The chromaticity coordinates (x, y) shall be calculated from the

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