ISO 21446:2019

Infant formula and adult nutritionals — Determination of trans and total (cis + trans) vitamin K1 content — Normal phase HPLC

This document specifies a method for the quantitative determination of trans and total (cis + trans) vitamin K1 in infant, pediatric and adult nutritionals using normal phase (NP) high-performance liquid chromatography (HPLC) with post-column reduction and fluorescence detection. The method demonstrated good linearity over a standard range of ~2 μg/l to 80 μg/l trans vitamin K1, and the limit of quantification (LOQ) was estimated to be 0,4 μg/l for standards and 0,09 μg/100 g ready to feed (RTF) for samples assuming 4 grams of sample are diluted to 10 ml.

Formules infantiles et produits nutritionnels pour adultes — Détermination de la teneur en vitamine K1 trans et totale (cis + trans) — Chromatographie liquide à haute performance (CLHP) en phase normale

General Information

• Status: Published
• Publication Date: 06-Jan-2019
• ICS: 67.050 - General methods of tests and analysis for food products
• Technical Committee: ISO/TC 34 - Food products
• Drafting Committee: ISO/TC 34/WG 14 - Vitamins, carotenoids and other nutrients
• Current Stage: 9060 - Close of review
• Start Date: 02-Sep-2029

Standards Content (Sample)

INTERNATIONAL ISO
STANDARD 21446
First edition
2019-01
Infant formula and adult
nutritionals — Determination of trans
and total (cis + trans) vitamin K
1
content — Normal phase HPLC
Formules infantiles et produits nutritionnels pour adultes —
Détermination de la teneur en vitamine K trans et totale (cis +
1
trans) — Chromatographie liquide à haute performance (CLHP) en
phase normale
Reference number
ISO 21446:2019(E)
©
ISO 2019

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ISO 21446:2019(E)

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© ISO 2019
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ii © ISO 2019 – All rights reserved

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ISO 21446:2019(E)

Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagents and materials . 2
6 Standard and solution preparation . 2
6.1 Mixture with volume fraction of 10 % isopropanol in iso-octane . 2
6.2 Mobile phase . 2
6.3 Post-column electrolyte solution . 3
6.4 Vitamin K (phytonadione) stock standard solution . 3
1
6.4.1 Vitamin K (phytonadione) stock standard solution . 3
1
6.4.2 Stock standard concentration (for non USP standard) . 3
6.5 Vitamin K (phytonadione) intermediate I standard solution . 3
1
6.6 Vitamin K (phytonadione) intermediate II standard solution . 3
1
6.7 Vitamin K (phytonadione) working standard solutions . 3
1
7 Apparatus . 4
8 Procedure. 4
8.1 Sample preparation . 4
8.1.1 Liquid samples .
...