SIST EN ISO 6887-1:2017/oprA1:2023

Microbiology of the food chain - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 1: General rules for the preparation of the initial suspension and decimal dilutions - Amendment 1: Requirements and guidance on the use of larger test portion size for qualitative methods (ISO 6887-1:2017/DAM1:2023)

Mikrobiologie der Lebensmittelkette - Vorbereitung von Untersuchungsproben und Herstellung von Erstverdünnungen und von Dezimalverdünnungen für mikrobiologische Untersuchungen - Teil 1: Allgemeine Regeln für die Herstellung von Erstverdünnungen und Dezimalverdünnungen - Änderung 1: Anforderungen und Leitlinien für die Verwendung größerer Prüfmengen bei qualitativen Verfahren (ISO 6887 1:2017/DAM 1:2023)

Microbiologie de la chaîne alimentaire - Préparation des échantillons, de la suspension mère et des dilutions décimales en vue de l'examen microbiologique - Partie 1: Règles générales pour la préparation de la suspension mère et des dilutions décimales - Amendement 1: Exigences et recommandations sur l’utilisation d’une taille de prise d’essai plus grande pour les méthodes qualitatives (ISO 6887-1:2017/DAM1:2023)

Mikrobiologija v prehranski verigi - Priprava preskusnih vzorcev, osnovne suspenzije in decimalnih razredčin za mikrobiološko preiskavo - 1. del: Splošna pravila za pripravo osnovne suspenzije in decimalnih razredčin - Dopolnilo A1: Zahteve in navodila o uporabi testnega vzorca večje velikosti za kvalitativne metode (ISO 6887-1:2017/DAM1:2023)

General Information

• Status: Not Published
• Public Enquiry End Date: 19-Oct-2023
• ICS: 07.100.30 - Food microbiology
• Technical Committee: KŽP - Agricultural food products
• Current Stage: 4020 - Public enquire (PE) (Adopted Project)
• Start Date: 11-Aug-2023
• Due Date: 29-Dec-2023
• Ref Project: EN ISO 6887-1:2017/prA1 - Microbiology of the food chain - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 1: General rules for the preparation of the initial suspension and decimal dilutions - Amendment 1: Requirements and guidance on the use of larger test portion size for qualitative methods (ISO 6887-1:2017/DAM1:2023)

Relations

• Amends: SIST EN ISO 6887-1:2017 - Microbiology of the food chain - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 1: General rules for the preparation of the initial suspension and decimal dilutions (ISO 6887-1:2017)
• Effective Date: 23-Jun-2021

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN ISO 6887-1:2017/oprA1:2023
01-oktober-2023
Mikrobiologija v prehranski verigi - Priprava preskusnih vzorcev, osnovne
suspenzije in decimalnih razredčin za mikrobiološko preiskavo - 1. del: Splošna
pravila za pripravo osnovne suspenzije in decimalnih razredčin - Dopolnilo A1:
Zahteve in navodila o uporabi testnega vzorca večje velikosti za kvalitativne
metode (ISO 6887-1:2017/DAM1:2023)
Microbiology of the food chain - Preparation of test samples, initial suspension and
decimal dilutions for microbiological examination - Part 1: General rules for the
preparation of the initial suspension and decimal dilutions - Amendment 1: Requirements
and guidance on the use of larger test portion size for qualitative methods (ISO 6887-
1:2017/DAM1:2023)
Mikrobiologie der Lebensmittelkette - Vorbereitung von Untersuchungsproben und
Herstellung von Erstverdünnungen und von Dezimalverdünnungen für mikrobiologische
Untersuchungen - Teil 1: Allgemeine Regeln für die Herstellung von Erstverdünnungen
und Dezimalverdünnungen - Änderung 1: Anforderungen und Leitlinien für die
Verwendung größerer Prüfmengen bei qualitativen Verfahren (ISO 6887 1:2017/DAM
1:2023)
Microbiologie de la chaîne alimentaire - Préparation des échantillons, de la suspension
mère et des dilutions décimales en vue de l'examen microbiologique - Partie 1: Règles
générales pour la préparation de la suspension mère et des dilutions décimales -
Amendement 1: Exigences et recommandations sur l’utilisation d’une taille de prise
d’essai plus grande pour les méthodes qualitatives (ISO 6887-1:2017/DAM1:2023)
Ta slovenski standard je istoveten z: EN ISO 6887-1:2017/prA1
ICS:
07.100.30 Mikrobiologija živil Food microbiology
SIST EN ISO 6887-1:2017/oprA1:2023 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN ISO 6887-1:2017/oprA1:2023

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SIST EN ISO 6887-1:2017/oprA1:2023
DRAFT AMENDMENT
ISO 6887-1:2017/DAM 1
ISO/TC 34/SC 9 Secretariat: AFNOR
Voting begins on: Voting terminates on:
2023-07-31 2023-10-23
Microbiology of the food chain — Preparation of test
samples, initial suspension and decimal dilutions for
microbiological examination —
Part 1:
General rules for the preparation of the initial suspension
and decimal dilutions
AMENDMENT 1: Requirements and guidance on the use of
larger test portion size for qualitative methods
ICS: 07.100.30
This document is circulated as received from the committee secretariat.
THIS DOCUMENT IS A DRAFT CIRCULATED
FOR COMMENT AND APPROVAL. IT IS
ISO/CEN PARALLEL PROCESSING
THEREFORE SUBJECT TO CHANGE AND MAY
NOT BE REFERRED TO AS AN INTERNATIONAL
STANDARD UNTIL PUBLISHED AS SUCH.
IN ADDITION TO THEIR EVALUATION AS
BEING ACCEPTABLE FOR INDUSTRIAL,
TECHNOLOGICAL, COMMERCIAL AND
USER PURPOSES, DRAFT INTERNATIONAL
STANDARDS MAY ON OCCASION HAVE TO
BE CONSIDERED IN THE LIGHT OF THEIR
POTENTIAL TO BECOME STANDARDS TO
WHICH REFERENCE MAY BE MADE IN
Reference number
NATIONAL REGULATIONS.
ISO 6887-1:2017/DAM 1:2023(E)
RECIPIENTS OF THIS DRAFT ARE INVITED
TO SUBMIT, WITH THEIR COMMENTS,
NOTIFICATION OF ANY RELEVANT PATENT
RIGHTS OF WHICH THEY ARE AWARE AND TO
PROVIDE SUPPORTING DOCUMENTATION. © ISO 2023

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SIST EN ISO 6887-1:2017/oprA1:2023
ISO 6887-1:2017/DAM 1:2023(E)
DRAFT AMENDMENT
ISO 6887-1:2017/DAM 1
ISO/TC 34/SC 9 Secretariat: AFNOR
Voting begins on: Voting terminates on:

Microbiology of the food chain — Preparation of test
samples, initial suspension and decimal dilutions for
microbiological examination —
Part 1:
General rules for the preparation of the initial suspension
and decimal dilutions
AMENDMENT 1: Requirements and guidance on the use of
larger test portion size for qualitative methods
ICS: 07.100.30
This document is circulated as received from the committee secretariat.
COPYRIGHT PROTECTED DOCUMENT
THIS DOCUMENT IS A DRAFT CIRCULATED
FOR COMMENT AND APPROVAL. IT IS
© ISO 2023
ISO/CEN PARALLEL PROCESSING
THEREFORE SUBJECT TO CHANGE AND MAY
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
NOT BE REFERRED TO AS AN INTERNATIONAL
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on STANDARD UNTIL PUBLISHED AS SUCH.
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
IN ADDITION TO THEIR EVALUATION AS
or ISO’s member body in the country of the requester. BEING ACCEPTABLE FOR INDUSTRIAL,
TECHNOLOGICAL, COMMERCIAL AND
ISO copyright office
USER PURPOSES, DRAFT INTERNATIONAL
CP 401 • Ch. de Blandonnet 8
STANDARDS MAY ON OCCASION HAVE TO
BE CONSIDERED IN THE LIGHT OF THEIR
CH-1214 Vernier, Geneva
POTENTIAL TO BECOME STANDARDS TO
Phone: [phone]
WHICH REFERENCE MAY BE MADE IN
Reference number
Email: [e-mail]
NATIONAL REGULATIONS.
Website: www.iso.org ISO 6887-1:2017/DAM 1:2023(E)
RECIPIENTS OF THIS DRAFT ARE INVITED
Published in Switzerland
TO SUBMIT, WITH THEIR COMMENTS,
NOTIFICATION OF ANY RELEVANT PATENT
RIGHTS OF WHICH THEY ARE AWARE AND TO
ii
  © ISO 2023 – All rights reserved
PROVIDE SUPPORTING DOCUMENTATION. © ISO 2023

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SIST EN ISO 6887-1:2017/oprA1:2023
ISO 6887-1:2017/DAM 1:2023(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).
ISO draws attention to the possibility that the implementation of this document may involve the use
of (a) patent(s). ISO takes no position concerning the evidence, validity or applicability of any claimed
patent rights in respect thereof. As of the date of publication of this document, ISO had received notice of
(a) patent(s) which may be required to implement this document. However, implementers are cautioned
that this may not represent the latest information, which may be obtained from the patent database
available at www.iso.org/patents. ISO shall not be held responsible for identifying any or all such patent
rights.
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For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO's adherence to the
World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see the following
URL: www.iso.org/iso/foreword.html.
This document was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 9,
Microbiology in collaboration with the European Committee for Standardization (CEN) Technical
Committee CEN/TC 463, Microbiology of the food chain, in accordance with the Agreement on technical
cooperation between ISO and CEN (Vienna Agreement).
A list of parts in the ISO 6887 series can be found on the ISO website.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www.iso.org/members.html.
iii
© ISO 2023 – All rights reserved

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SIST EN ISO 6887-1:2017/oprA1:2023

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SIST EN ISO 6887-1:2017/oprA1:2023
ISO 6887-1:2017/DAM 1:2023(E)
Microbiology of the food chain — Preparation of test
samples, initial suspension and decimal dilutions for
microbiological examination —
Part 1:
General rules for the preparation of the initial suspension
and decimal dilutions
AMENDMENT 1: Requirements and guidance on the use of
larger test portion size for qualitative methods

3.2
Replace the text with the following:
3.2
composite sample
mixed sample of a number of the same items of food, animal feed, animals or environment, prepared in
or out of the laboratory from which a test portion is taken for examination.
Note 1 to entry: See Table A.1 for illustration of a composite sample.

3.3
Replace the text with the following:
3.3
pooled sample
mixed sample of a number of the same items of food, animal feed, animals or environment, prepared
in or out of the laboratory where the complete mixture is the test portion and is taken as a whole for
examination.

Add after 3.10 the following:
3.11
category
group of sample types of the same origin
EXAMPLE Heat-processed milk and dairy products.
[SOURCE: ISO 16140-1:2016]
3.12
type
for a given category, a group of items processed in a similar way, with similar intrinsic characteristics
and a similar microbial ecology
EXAMPLE Food category: heat-processed milk and dairy products; food type: pasteurized dairy product.
1
© ISO 2023 – All rights reserved

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SIST EN ISO 6887-1:2017/oprA1:2023
ISO 6887-1:2017/DAM 1:2023(E)
[SOURCE: ISO 16140-1:2016]
3.13
item
single specified food, feed, environmental, or primary production matrix
EXAMPLE Food category: heat-processed milk and dairy products; food type: pasteurized dairy product;
food item: milk-based desserts.
[SOURCE: ISO 16140-1:2016]
3.14
larger test portion
measured (volume or mass) representative sample taken from the laboratory sample (3.1) or test
sample (3.4) for use in the preparation of the initial suspension that is larger than the test portion size
that has been described in the original method and/or validation document

9.3
Replace the text with the following:
9.3  Composite sample and larger test portion
9.3.1  Composite sample
A composite sample is where a number of the same items are mixed, and a test portion is taken for
examination in the laboratory as illustrated in Annex A (Table A.1). The size of the test portion removed
from the composite sample will remain the same as described in the original method and/or validation
document.
Compositing shall be applicable to qualitative tests only.
A number of items may be composited at the sampling stage by the client (out of the laboratory) or by
the laboratory (at client’s request). Only items from the same origin or source (e. g. same batch or lot)
shall be composited.
9.3.2  Larger test portion
A larger test portion is a sample that is larger than the test portion size that has been described in the
original method and/or validation document.
Testing a larger test portion may be necessary 1) to reflect microbiological quality of a large batch
of product, 2) in case when a large number of environmental samples are taken or 3) as sometimes
required by national or regional legislation.
A larger test portion can originate from a single larger test portion (9.3.2.1) or from the pooling of
samples (9.3.2.2).
If a laboratory sample larger than the maximum sample size has been submitted, the sample can be
split into multiple test portions based on the maximum sample size (e.g. if a laboratory sample of 750 g
has been received for Salmonella testing it can be prepared as two 375 g test portions).
9.3.2.1  Larger single test portion
A larger single test portion is a sample that is larger than the test portion size that has been described
in the original method and/or validation document originating from a single sample. A larger single test
portion can be applied to both qualitative and quantitative testing.
9.3.2.2  Pooled sample
...