SIST EN 17716:2025
(Main)Plant biostimulants - Determination of Escherichia coli
Plant biostimulants - Determination of Escherichia coli
This European Standard establishes the methodology for the determination of Escherichia coli in plant biostimulant products in accordance to the future Regulation of EU fertilising products.
Pflanzen-Biostimulanzien - Bestimmung von Escherichia coli
Dieses Dokument enthält allgemeine Leitlinien für den Nachweis und die Identifizierung des festgelegten Mikroorganismus Escherichia coli in technischen und formulierten Produkten von Biostimulanzien (sowohl in flüssigem als auch in festem Zustand) sowie das horizontale Verfahren für die Zählung von β Glucuronidase-positiven Escherichia coli in Produkten von Pflanzen-Biostimulanzien (sowohl in flüssigem als auch in festem Zustand).
Das in diesem Dokument beschriebene qualitative Verfahren basiert auf dem Nachweis von Escherichia coli in einem nicht-selektiven flüssigen Medium (Anreicherungsbouillon), gefolgt von der Isolierung auf einem selektiven Agar. Je nach gewünschtem Nachweisgrad können auch andere Methoden geeignet sein.
ANMERKUNG Für den Nachweis von Escherichia coli können Subkulturen auf nicht-selektiven Nährmedien durchgeführt werden, gefolgt von geeigneten Identifizierungsschritten (z. B. unter Verwendung von Identifizierungskits).
Das in diesem Dokument beschriebene quantitative Verfahren verwendet ein Koloniezählverfahren bei 44 °C auf einem festen Medium, das einen chromogenen Bestandteil zum Nachweis des Enzyms β Glucuronidase enthält.
WARNUNG — Stämme von Escherichia coli, die bei 44 °C nicht wachsen, und insbesondere solche, die β Glucuronidase-negativ sind, wie z. B. Escherichia coli O157, werden nicht erkannt.
Biostimulants des végétaux - Détermination des Escherichia coli
Le présent document donne des lignes directrices générales pour la détection et l’identification du microorganisme spécifié Escherichia coli dans les produits biostimulants techniques et formulés, aussi bien à l’état liquide qu’à l’état solide, ainsi que la méthode horizontale pour le dénombrement des Escherichia coli ß-glucuronidase-positive dans les produits biostimulants des végétaux (aussi bien à l’état liquide qu’à l’état solide).
La méthode qualitative décrite dans le présent document repose sur la détection des Escherichia coli dans un milieu liquide non sélectif (bouillon d’enrichissement), suivie de l’isolement sur un milieu gélosé sélectif. D’autres méthodes peuvent être appropriées, en fonction du niveau de détection requis.
NOTE Pour la détection des Escherichia coli, des subcultures peuvent être effectuées sur des milieux de culture non sélectifs, suivies d’étapes d’identification appropriées (par exemple, à l’aide de kits d’identification).
La méthode quantitative décrite dans le présent document utilise une technique de dénombrement des colonies à 44 °C sur un milieu solide contenant un ingrédient chromogène pour la détection de l’enzyme ß-glucuronidase.
AVERTISSEMENT - Certaines souches d’Escherichia coli qui ne poussent pas à 44 °C et, en particulier, celles qui sont ß-glucuronidase-négative, telles que les Escherichia coli O157, ne seront pas mises en évidence.
Rastlinski biostimulanti - Določanje Escherichia coli
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-februar-2025
Nadomešča:
SIST-TS CEN/TS 17716:2023
Rastlinski biostimulanti - Določanje Escherichia coli
Plant biostimulants - Determination of Escherichia coli
Pflanzen-Biostimulanzien - Bestimmung von Escherichia coli
Biostimulants des végétaux - Détermination des Escherichia coli
Ta slovenski standard je istoveten z: EN 17716:2024
ICS:
65.080 Gnojila Fertilizers
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN 17716
EUROPEAN STANDARD
NORME EUROPÉENNE
November 2024
EUROPÄISCHE NORM
ICS 65.080 Supersedes CEN/TS 17716:2022
English Version
Plant biostimulants - Determination of Escherichia coli
Biostimulants des végétaux - Détermination des Pflanzen-Biostimulanzien - Bestimmung von
Escherichia coli Escherichia coli
This European Standard was approved by CEN on 26 August 2024.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATIO N
EUROPÄISCHES KOMITEE FÜR NORMUN G
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2024 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 17716:2024 E
worldwide for CEN national Members.
Contents Page
European foreword . 4
Introduction . 5
1 Scope . 6
2 Normative references . 6
3 Terms and definitions . 7
4 Principle . 7
4.1 Qualitative method . 7
4.2 Quantitative method . 7
5 Diluent and culture media . 8
5.1 General . 8
5.2 Broth and culture media in the qualitative method . 8
5.2.1 Enrichment broth . 8
5.2.2 Selective culture media: Tryptone-Bile-Glucuronic agar (TBX) for isolation of E. coli 8
5.3 Diluent and culture media in the quantitative method . 8
5.3.1 Diluent . 8
5.3.2 Culture media: Tryptone-Bile-Glucuronic agar (TBX) . 8
6 Apparatus and glassware . 8
7 Handling of plant biostimulants and sampling . 8
8 Procedure. 9
8.1 General . 9
8.2 Qualitative method . 9
8.2.1 General . 9
8.2.2 Incubation of the inoculated enrichment broth. 9
8.2.3 Detection and identification of E. coli . 9
8.3 Quantitative method . 10
8.3.1 Test portion and initial suspension . 10
8.3.2 Serial dilutions . 10
8.3.3 Inoculation (surface plate method) and incubation . 10
8.3.4 Counting the colony-forming units . 11
9 Expression of results . 11
9.1 Expression of results in the qualitative test . 11
9.2 Expression of results in the quantitative test . 11
10 Test report . 12
Annex A (normative) Composition and preparation of culture media and reagents . 13
A.1 General . 13
A.2 Enrichment broth . 13
A.3 Other enrichment broth . 14
A.4 Selective agar for isolation . 14
A.5 Other selective agar for isolation . 15
A.6 Selective agar for confirmation . 16
Annex B (normative) Neutralization of the antimicrobial properties of the product . 17
B.1 General . 17
B.2 Suitability of the detection method . 17
B.3 Interpretation of suitability test results . 18
Annex C (informative) Interlaboratory study . 19
C.1 Materials used in the interlaboratory study . 19
C.2 Results of the interlaboratory study . 20
Annex ZA (informative) Relationship of this European Standard and the essential
requirements of Regulation (EU) 2019/1009 making available on the market of EU
fertilising products aimed to be covered . 22
Bibliography . 23
European foreword
This document (EN 17716:2024) has been prepared by Technical Committee CEN/TC 455 “Plant
biostimulants”, the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by May 2025, and conflicting national standards shall be
withdrawn at the latest by May 2025.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes CEN/TS 17716:2022.
— the Introduction has been updated and Table 1 has been removed;
— normative references have been updated;
— in Clause 3, new terms and definitions have been added and others have been revised;
— Annexes A, B and C have been revised.
— Annex ZA has been added.
This document has been prepared under a standardization request addressed to CEN by the European
Commission. The Standing Committee of the EFTA States subsequently approves these requests for its
Member States.
For the relationship with EU Legislation, see informative Annex ZA, which is an integral part of this
document.
Any feedback and questions on this document should be directed to the users’ national standards body.
A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organisations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia,
Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland,
Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of North
Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the United
Kingdom.
Introduction
The European Committee for Standardization (CEN) was requested by the European Commission (EC) to
draft European Standards or European Standardization deliverables to support the implementation of
Regulation (EU) 2019/1009 of 5 June 2019 [1] laying down rules on the making available on the market
of EU fertilising products (“FPR” or “Fertilising Products Regulation”).
This standardization request, presented as SR M/564 and relevant amendments, also contributes to the
Communication on “Innovating for Sustainable Growth: A Bio economy for Europe”. The interest in plant
biostimulants has increased significantly in Europe as a valuable tool to use in agriculture.
Standardization was identified as having an important role in order to promote the use of biostimulants.
The work of CEN/TC 455 seeks to improve the reliability of the supply chain, thereby improving the
confidence of farmers, industry, and consumers in biostimulants, and will promote and support
commercialisation of the European biostimulant industry.
WARNING — Persons using this document should be familiar with normal laboratory practice. This
document does not purport to address all of the safety problems, if any, associated with its use. It is the
responsibility of the user to establish appropriate safety and health practices and to ensure compliance
with any national regulatory conditions.
IMPORTANT — It is absolutely essential that tests conducted in accordance with this document be
carried out by suitably trained staff.
1 Scope
This document gives guidance for the detection and identification of the specified microorganism
Escherichia coli in technical and formulated plant biostimulants, both in liquid and solid states, and also
the horizontal method for the enumeration of ß-glucuronidase-positive E. coli in plant biostimulants
(both in liquid and solid states).
The qualitative method described in this document is based on the detection of E. coli in a non-selective
liquid medium (enrichment broth), followed by isolation on a selective agar. Other methods can be
appropriate, depending on the level of detection required.
NOTE 1 For the detection of E. coli, subcultures can be performed on non-selective culture media followed by
suitable identification steps (e.g. using identification kits).
The quantitative method described in this document uses a colony-count technique at 44 °C ± 1 °C on a
solid medium containing a chromogenic ingredient for detection of the enzyme ß-glucuronidase.
NOTE 2 Strains of E. coli which do not grow at 44 °C ± 1 °C and, in particular, those that are ß-glucuronidase
negative, such as E. coli O157, will not be dete
...
Questions, Comments and Discussion
Ask us and Technical Secretary will try to provide an answer. You can facilitate discussion about the standard in here.