SIST EN 17701-3:2025
(Main)Plant biostimulants - Determination of specific elements - Part 3: Determination of mercury
Plant biostimulants - Determination of specific elements - Part 3: Determination of mercury
This European Standard specifies a method for determination of the content of mercury (Hg) in plant biostimulants using (cold) vapour generation apparatus coupled to an atomic absorption spectrophotometer and a method using a direct amalgamation technique. It is applicable to aqua regia digests prepared according to CEN/TS 17701-1.
NOTE It is also possible to use other suitable methods for the determination of mercury described in Annex A if users prove that the method gives the same results as the methods described in this Technical Specification.
This European Standard is also applicable to the blends of fertilizing products where plant biostimulants are the main part of the blend. Otherwise, the Technical Specification for the main part of the blend apply.
Pflanzen-Biostimulanzien - Bestimmung spezifischer Elemente - Teil 3: Bestimmung von Quecksilber
Dieses Dokument legt ein Verfahren zur Bestimmung des Quecksilbergehalts (Hg) in Pflanzen-Biostimulanzien mittels eines Systems der (Kalt-)Verdampfungstechnik, gekoppelt mit einem Atom-absorptionsspektrometer und einem Verfahren, das auf einer direkten Amalgamierungstechnik beruht, fest. Es ist für Königswasseraufschlüsse anwendbar, die nach EN 17701 1:—1 hergestellt werden.
ANMERKUNG Auch die Anwendung anderer, in Anhang A beschriebener, geeigneter Verfahren der Quecksilber-bestimmung ist möglich, sofern die Anwender nachweisen, dass das Verfahren zu gleichen Ergebnissen führt wie die in diesem Dokument beschriebenen Verfahren.
Dieses Dokument ist auch für die Mischungen von Düngeprodukten anwendbar, in denen die Pflanzen-Biostimulanzien der Hauptbestandteil der Mischung sind. Andernfalls gilt das Verfahren für den Haupt-bestandteil der Mischung.
Biostimulants des végétaux - Dosage des éléments spécifiques - Partie 3 : Dosage du mercure
Le présent document spécifie une méthode pour la détermination de la teneur en mercure (Hg) dans les biostimulants des végétaux avec un appareil de génération de vapeur (à froid) couplé à un spectrophotomètre d’absorption atomique et une méthode utilisant une technique d’amalgamation directe. Il est applicable aux digestats à l’eau régale préparés conformément à l’EN 17701 1:—1.
NOTE Il est aussi possible d’utiliser d’autres méthodes adaptées pour le dosage du mercure décrites dans l’Annexe A, si l’utilisateur prouve que la méthode conduit aux mêmes résultats que les méthodes décrites dans le présent document.
Le présent document est également applicable aux mélanges de fertilisants dont la majeure partie est constituée de biostimulants des végétaux. Dans le cas contraire, la méthode relative au constituant principal du mélange s’applique.
Rastlinski biostimulanti - Določanje specifičnih elementov - 3. del: Določanje živega srebra
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-februar-2025
Nadomešča:
SIST-TS CEN/TS 17701-3:2023
Rastlinski biostimulanti - Določanje specifičnih elementov - 3. del: Določanje
živega srebra
Plant biostimulants - Determination of specific elements - Part 3: Determination of
mercury
Pflanzen-Biostimulanzien - Bestimmung spezifischer Elemente - Teil 3: Bestimmung von
Quecksilber
Biostimulants des végétaux - Dosage des éléments spécifiques - Partie 3 : Dosage du
mercure
Ta slovenski standard je istoveten z: EN 17701-3:2024
ICS:
65.080 Gnojila Fertilizers
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN 17701-3
EUROPEAN STANDARD
NORME EUROPÉENNE
November 2024
EUROPÄISCHE NORM
ICS 65.080 Supersedes CEN/TS 17701-3:2022
English Version
Plant biostimulants - Determination of specific elements -
Part 3: Determination of mercury
Biostimulants des végétaux - Dosage des éléments Pflanzen-Biostimulanzien - Bestimmung spezifischer
spécifiques - Partie 3 : Dosage du mercure Elemente - Teil 3: Bestimmung von Quecksilber
This European Standard was approved by CEN on 26 August 2024.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2024 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 17701-3:2024 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle . 5
4.1 Vapour generation atomic absorption spectrophotometry (VG-AAS) . 5
4.2 Direct amalgamation (DA) . 6
5 Interferences . 6
5.1 Vapour generation atomic absorption spectrophotometry (VG-AAS) . 6
5.2 Direct amalgamation (DA) . 6
6 Reagents . 6
7 Apparatus . 7
8 Procedure . 8
8.1 Preparation of the test and blank solutions . 8
8.2 Preparation of the calibration solutions . 8
8.3 Measurement . 8
8.3.1 Instrument conditions . 8
8.3.2 Matrix effects . 8
8.3.3 Spiking . 9
8.3.4 Measurement by VG-AAS . 9
8.3.5 Measurement by DA . 9
9 Calculation and expression of the results . 10
10 Test report . 10
Annex A (informative) Methods for mercury determination . 11
A.1 Cold vapour generation (hydride generation) . 11
A.2 Direct amalgamation technique . 12
A.3 Inductively coupled plasma mass spectrometry (ICP-MS) . 12
Annex B (informative) Results of the inter-laboratory study . 13
B.1 Inter-laboratory tests . 13
B.2 Statistical results . 13
Annex ZA (informative) Relationship of this European Standard and the essential
requirements of Regulation (EU) 2019/1009 making available on the market of EU
fertilising products aimed to be covered . 15
Bibliography . 16
European foreword
This document (EN 17701-3:2024) has been prepared by Technical Committee CEN/TC 455 “Plant
biostimulants”, the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by May 2025, and conflicting national standards shall be
withdrawn at the latest by May 2025.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes CEN/TS 17701-3:2022.
CEN/TS 17701-3:2022:
— scope text regarding blends has been changed;
— Annex B “Results of the inter-laboratory study” has been added;
— Annex ZA has been added.
This document has been prepared under a standardization request addressed to CEN by the European
Commission. The Standing Committee of the EFTA States subsequently approves these requests for its
Member States.
For the relationship with EU Legislation, see informative Annex ZA, which is an integral part of this
document.
EN 17701 series, Plant biostimulants — Determination of specific elements, consists of the following parts:
— Part 1: Digestion by aqua regia for subsequent determination of elements;
— Part 2: Determination of total content of Cd, Pb, Ni, As, Cr, Cu and Zn;
— Part 3: Determination of mercury.
Any feedback and questions on this document should be directed to the users’ national standards body.
A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organisations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia,
Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland,
Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of North
Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the United
Kingdom.
Introduction
The European Committee for Standardization (CEN) was requested by the European Commission (EC) to
draft European Standards or European Standardization deliverables to support the implementation of
Regulation (EU) 2019/1009 of 5 June 2019 [1] laying down rules on the making available on the market
of EU fertilising products (“FPR” or “Fertilising Products Regulation”).
This standardization request, presented as SR M/564 and relevant amendments, also contributes to the
Communication on “Innovating for Sustainable Growth: A Bio economy for Europe”. The interest in plant
biostimulants has increased significantly in Europe as a valuable tool to use in agriculture.
Standardization was identified as having an important role in order to promote the use of biostimulants.
The work of CEN/TC 455 seeks to improve the reliability of the supply chain, thereby improving the
confidence of farmers, industry, and consumers in biostimulants, and will promote and support
commercialisation of the European biostimulant industry. This document concerns the analytical
measurement step for the determination of mercury in plant biostimulants after digestion by aqua regia
according to EN 17701-1:2024. It covers cold vapour generation followed by mercury determination
using atomic absorption spectrophotometry (AAS). Different cold vapour generation techniques can be
used (flow injection, segmented flow, batch) [2]. This document also includes a method based on a direct
amalgamation technique which is widely used in many analytical laboratories [3]. It is also possible to
use other suitable methods of mercury determination described in Annex A if users prove that the
methods give the same results as the methods described in this document. Standards for determination
of mercury in sludge, soil and treated biowaste [4] and for fertilizers and liming materials [5] were
studied and considered as a basis of this document.
The inter-laboratory study reflects the final statistical characteristics of the method for the determination
of mercury in aqua regia digests including both, the digestion and the measurement steps.
WARNING — Persons using this document should be familiar with usual laboratory practice. This
document does not purport to address all of the safety issues, if any, associated with its use. It is the
responsibility of the user to establish appropriate health and safety practices and to ensure compliance
with any national regulatory conditions.
IMPORTANT — It is absolutely essential that tests conducted according to this document are carried out
by suitably trained staff.
1 Scope
This document specifies a method for determination of the content of mercury (Hg) in plant biostimulants
using (cold) vapour generation apparatus coupled to an atomic absorption spectrophotometer and a
method using a direct amalgamation technique. It is applicable to aqua regia digests prepared according
to EN 17701-1:2024.
NOTE It is also possible to use other suitable methods for the determination of mercury described in Annex A
if users prove that the method gives the same results as the methods described in this document.
This document is applicable to the blends of fertilizing products where a blend is a mix of at least two of
the following component EU fertilising products categories: Fertilizers, Liming Materials, Soil Improvers,
Growing Media, Inhibitors, Plant Biostimulants, and where the following category Plant Biostimulants is
the highest % in the blend by mass or volume, or in the case of liquid form by dry mass. If Plant
Biostimulants is not the highest % in the blend, the European Standard for the highest % of the blend
applies. In case a blend of fertilizing products is composed of components in equal quantity or in case the
component EU fertilising products used for the blend have identical formulations , the user decides
which standard to apply.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
EN 17701-1:2024, Plant biostimulants — Determination of specific elements — Part 1: Digestion by aqua
regia for subsequent determination of elements
EN 17701-2:2024, Plant biostimulants — Determination of specific elements — Part 2: Determination of
total content of Cd, Pb, Ni, As, Cr, Cu and
...
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