oSIST prEN 1104:2025
(Main)Paper and board intended to come into contact with foodstuffs - Determination of the transfer of antimicrobial constituents
Paper and board intended to come into contact with foodstuffs - Determination of the transfer of antimicrobial constituents
This document specifies a test method for the determination of the transfer of antimicrobial constituents from paper and board materials and articles intended for food contact.
NOTE The use of this document is determined by legislation pertaining to paper and board intended to come into contact with foodstuffs.
Papier und Pappe vorgesehen für den Kontakt mit Lebensmitteln - Bestimmung des Übergangs antimikrobieller Bestandteile
Dieses Dokument legt ein Prüfverfahren zur Bestimmung der Übertragung antimikrobieller Bestandteile von Materialien und Artikeln aus Papier und Pappe fest, die für den Kontakt mit Lebensmitteln vorgesehen sind.
ANMERKUNG Die Anwendung dieses Dokuments ist durch die Vorschriften für Papier und Pappe, die für den Kontakt mit Lebensmitteln vorgesehen sind, festgelegt.
Papier et carton destinés à entrer en contact avec les denrées alimentaires - Détermination du transfert des constituants antimicrobiens
Le présent document spécifie une méthode d’essai pour la détermination du transfert des constituants antimicrobiens des matériaux en papier et carton et articles destinés à entrer en contact avec des denrées alimentaires.
NOTE L’utilisation du présent document est spécifiée par la législation relative au papier et au carton destinés à entrer en contact avec des denrées alimentaires.
Papir, karton in lepenka v neposrednem stiku z živili - Določanje izločanja antimikrobnih snovi
General Information
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Standards Content (Sample)
SLOVENSKI STANDARD
01-december-2025
Papir, karton in lepenka v neposrednem stiku z živili - Določanje izločanja
antimikrobnih snovi
Paper and board intended to come into contact with foodstuffs - Determination of the
transfer of antimicrobial constituents
Papier und Pappe vorgesehen für den Kontakt mit Lebensmitteln - Bestimmung des
Übergangs antimikrobieller Bestandteile
Papier et carton destinés à entrer en contact avec les denrées alimentaires -
Détermination du transfert des constituants antimicrobiens
Ta slovenski standard je istoveten z: prEN 1104
ICS:
67.250 Materiali in predmeti v stiku z Materials and articles in
živili contact with foodstuffs
85.060 Papir, karton in lepenka Paper and board
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
DRAFT
EUROPEAN STANDARD
NORME EUROPÉENNE
EUROPÄISCHE NORM
October 2025
ICS 67.250; 85.060 Will supersede EN 1104:2018
English Version
Paper and board intended to come into contact with
foodstuffs - Determination of the transfer of antimicrobial
constituents
Papier et carton destinés à entrer en contact avec les Papier und Pappe vorgesehen für den Kontakt mit
denrées alimentaires - Détermination du transfert des Lebensmitteln - Bestimmung des Übergangs
constituants antimicrobiens antimikrobieller Bestandteile
This draft European Standard is submitted to CEN members for enquiry. It has been drawn up by the Technical Committee
CEN/TC 172.
If this draft becomes a European Standard, CEN members are bound to comply with the CEN/CENELEC Internal Regulations
which stipulate the conditions for giving this European Standard the status of a national standard without any alteration.
This draft European Standard was established by CEN in three official versions (English, French, German). A version in any other
language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC
Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of which they are
aware and to provide supporting documentation.
Warning : This document is not a European Standard. It is distributed for review and comments. It is subject to change without
notice and shall not be referred to as a European Standard.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2025 CEN All rights of exploitation in any form and by any means reserved Ref. No. prEN 1104:2025 E
worldwide for CEN national Members.
Contents Page
European foreword . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle . 5
5 Apparatus . 5
6 Reagents and media . 6
6.1 General. 6
6.2 Non-ionic wetting agent. 6
6.3 Nutrient medium for the preparation of Bacillus subtilis spores . 6
6.3.1 Composition . 6
6.3.2 Preparation . 6
6.4 Sabouraud nutrient medium for the preparation of Aspergillus niger spores . 7
6.4.1 Composition . 7
6.4.2 Preparation . 7
6.5 Nutrient medium for the inhibition test with Bacillus subtilis . 7
6.5.1 Composition . 7
6.5.2 Preparation . 7
6.6 Modified Sabouraud nutrient medium for the inhibition test with Aspergillus niger . 7
6.6.1 Composition . 7
6.6.2 Preparation . 8
6.7 Tryptone salt solution . 8
6.7.1 Composition . 8
6.7.2 Preparation . 8
6.8 Test microorganisms . 8
6.8.1 General. 8
6.8.2 Preparation of working cultures . 8
6.8.3 Preparation inoculating spore suspension of Bacillus subtilis . 9
6.8.4 Preparation of inoculating spore suspension of Aspergillus niger . 9
6.8.5 Concentrations of spores of inhibition . 9
6.9 Positive controls . 9
6.9.1 Antibiotic . 9
6.9.2 Antifungal agent . 10
7 Sampling and preparation of test pieces . 10
8 Procedure . 10
8.1 General. 10
8.2 Preparation of plates . 10
8.2.1 General. 10
8.2.2 Test with Bacillus subtilis . 10
8.2.3 Test with Aspergillus niger . 11
8.3 Incubation. 11
9 Evaluation . 11
10 Test report . 12
Annex A (informative) Guidelines for interpreting results . 13
European foreword
This document (prEN 1104:2025) has been prepared by Technical Committee CEN/TC 172 “Pulp, paper
and board”, the secretariat of which is held by DIN.
This document is currently submitted to the CEN Enquiry.
This document will supersede EN 1104:2018.
This document includes the following significant technical changes with respect to EN 1104:2018:
a) 3.1 has been updated according to current regulations for terms and definitions;
b) Clause 5, A.3.2.3 and A.3.3.2: The statement that the measuring of the width of the inhibition zone is
not compulsory has been removed as the result requires to measure the width of the inhibition zone
in order to check the 2 mm criteria;
c) 5.3 and Clause 7: the precision regarding the punch iron has been updated; usage of the term
“aseptic” or “sterile” which both signifies germ-free;
d) 6.3.1, 6.4.1, 6.5.1, 6.7.1 regarding compositions of media: the term “tryptic digest” for the casein has
been deleted as some media indicate pancreatic digest which is the same thing;
e) 6.3.2, 6.4.2, 6.5.2, 6.6.2: unnecessary details regarding media containers have been deleted;
precision about storage conditions have been updated;
f) 6.8.2, 6.8.3, 6.8.4, 6.8.5: unnecessary details have been deleted and details for a better
understanding of the procedure have been added;
g) 6.8.5: the term “conidia” has been replaced by the term “spores” to harmonize wording since both
terms have the same meaning;
h) 6.9.2: the term “solution” for the amphotericin B has been deleted as it could be bought as pre-
impregnated discs; the note has been deleted as the isothiazolinone solution is not commercially
available;
i) Clause 7: the phrase “for each microorganism” has been deleted as 20 test pieces for both
microorganisms for one tested area is enough (9 test pieces by microorganism and by tested
area/side);
j) Clause 7 and Clause 8: a clarification about the sides tested has been added;
k) 8.2.2 and 8.2.3 have been rephrased for a better understanding;
l) 8.3: evaluation of the plates has been updated since it could be done at least 3 days after the end of
the incubation if the plates are stored at (5 ± 3) °C;
m) Clause 9: the precision “with a new inoculating suspension” has been deleted to allow repeating the
assay with the same inoculating suspension;
n) A.3.2.4: the term “positive” has been replaced by “negative” since the negative control is the nutrient
agar plate without test pieces and inoculated with the test microorganism.
1 Scope
This document specifies a test method for the determination of the transfer of antimicrobial
constituents from paper and board materials and articles intended for food contact.
NOTE The use of this document is determined by legislation pertaining to paper and board intended to come
into contact with foodstuffs.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
EN ISO 186, Paper and board — Sampling to determine average quality (ISO 186)
EN ISO 7218, Microbiology of the food chain — General requirements and guidance for microbiological
examinations (ISO 7218)
EN ISO 11133 , Microbiology of food, animal feed and water — Preparation, production, storage and
performance testing of culture media (ISO 11133)
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminology databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https://www.iso.org/obp/
— IEC Electropedia: available at https://www.electropedia.org/
3.1
inhibition zone
obvious area in which growth is absent and which forms around test pieces placed on a nutrient
medium inoculated with a preselected test microorganism, due to the release of water-soluble
antimicrobial constituents
Note 1 to entry: The proof of the presence
...
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