Plant biostimulants - Claims - Part 5: Determination of availability of confined nutrients in the soil or rhizosphere

The claim described in this document concerns the improvement of availability of confined nutrients in the soil or rhizosphere by a plant biostimulant.
This document is aimed primarily at manufacturers, laboratories, researchers, technical centres, companies that will put the products on market, notifying authorities, notified bodies, and market surveillance authorities.

Biostimulanzien für die pflanzliche Anwendung - Angaben - Teil 5: Verfügbarkeit von im Boden und in der Rhizosphäre enthaltenen Nährstoffen

Die in diesem Dokument beschriebene Produktangabe betrifft die Verbesserung der Verfügbarkeit von im Boden oder in der Rhizosphäre enthaltenen Nährstoffen durch ein Biostimulans für die pflanzliche Anwendung.
Dieses Dokument richtet sich in erster Linie an Hersteller, Labore, Forschungstätige, technische Zentren, Unternehmen, die die Produkte in Verkehr bringen werden, notifizierende Behörden, notifizierte Stellen und Marktüberwachungsbehörden.

Biostimulants des végétaux - Allégations - Partie 5 : Détermination de la disponibilité des éléments nutritifs confinés dans le sol ou la rhizosphère

L’allégation décrite dans le présent document concerne l’amélioration de la disponibilité des éléments nutritifs confinés dans le sol ou la rhizosphère grâce à un biostimulant des végétaux.
Le présent document est principalement destiné aux fabricants, aux laboratoires, aux chercheurs, aux centres techniques, aux sociétés qui vont mettre les produits sur le marché, aux autorités notifiantes, aux organismes notifiés et aux autorités de surveillance du marché.

Rastlinski biostimulanti - Navedbe - 5. del: Določanje razpoložljivosti hranil v tleh in rizosferi

Navedba, opisana v tem dokumentu, se navezuje na izboljšanje razpoložljivosti omejenih hranil v tleh ali rizosferi z rastlinskim biostimulantom.
Ta dokument je namenjen zlasti proizvajalcem, laboratorijem, raziskovalcem, tehničnim centrom, podjetjem, ki bodo dala izdelke na trg, priglasitvenim organom, priglašenim organom in organom za nadzor trga.

General Information

Status
Published
Public Enquiry End Date
30-Nov-2021
Publication Date
14-Dec-2022
Technical Committee
Current Stage
6060 - National Implementation/Publication (Adopted Project)
Start Date
14-Dec-2022
Due Date
18-Feb-2023
Completion Date
15-Dec-2022

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SLOVENSKI STANDARD
SIST-TS CEN/TS 17700-5:2023
01-januar-2023
Rastlinski biostimulanti - Navedbe - 5. del: Določanje razpoložljivosti hranil v tleh
in rizosferi
Plant biostimulants - Claims - Part 5: Determination of availability of confined nutrients in
the soil or rhizosphere
Biostimulanzien für die pflanzliche Anwendung - Angaben - Teil 5: Verfügbarkeit von im
Boden und in der Rhizosphäre enthaltenen Nährstoffen
Biostimulants des végétaux - Allégations - Partie 5 : Détermination de la disponibilité des
éléments nutritifs confinés dans le sol ou la rhizosphère
Ta slovenski standard je istoveten z: CEN/TS 17700-5:2022
ICS:
65.080 Gnojila Fertilizers
SIST-TS CEN/TS 17700-5:2023 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------
SIST-TS CEN/TS 17700-5:2023

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SIST-TS CEN/TS 17700-5:2023


CEN/TS 17700-5
TECHNICAL SPECIFICATION

SPÉCIFICATION TECHNIQUE

March 2022
TECHNISCHE SPEZIFIKATION
ICS 65.080
English Version

Plant biostimulants - Claims - Part 5: Determination of
availability of confined nutrients in the soil or rhizosphere
Biostimulants des végétaux - Allégations - Partie 5 : Pflanzen-Biostimulanzien - Angaben - Teil 5:
Détermination de la disponibilité des éléments nutritifs Verfügbarkeit von im Boden und in der Rhizosphäre
confinés dans le sol ou la rhizosphère enthaltenen Nährstoffen
This Technical Specification (CEN/TS) was approved by CEN on 3 January 2022 for provisional application.

The period of validity of this CEN/TS is limited initially to three years. After two years the members of CEN will be requested to
submit their comments, particularly on the question whether the CEN/TS can be converted into a European Standard.

CEN members are required to announce the existence of this CEN/TS in the same way as for an EN and to make the CEN/TS
available promptly at national level in an appropriate form. It is permissible to keep conflicting national standards in force (in
parallel to the CEN/TS) until the final decision about the possible conversion of the CEN/TS into an EN is reached.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2022 CEN All rights of exploitation in any form and by any means reserved Ref. No. CEN/TS 17700-5:2022 E
worldwide for CEN national Members.

---------------------- Page: 3 ----------------------
SIST-TS CEN/TS 17700-5:2023
CEN/TS 17700-5:2022 (E)
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Terminology of the claims . 6
4.1 Claims . 6
5 Assessment markers to validate the claim . 6
5.1 General considerations for markers measurements . 6
5.2 Soil or rhizosphere markers . 6
5.3 Plant markers . 7
6 Specifications for the performance of the trials . 8
6.1 General specifications . 8
6.2 Trial design . 8
Annex A (informative) Methods to measure the nutrient availability in soil or rhizosphere
. 9
Annex B (informative) Digestion and analysis of plant samples .10
Annex C (informative) Triangle of soil texture .11
Bibliography .12

2

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SIST-TS CEN/TS 17700-5:2023
CEN/TS 17700-5:2022 (E)
European foreword
This document (CEN/TS 17700-5:2022) has been prepared by Technical Committee CEN/TC 455 “Plant
biostimulants”, the secretariat of which is held by AFNOR.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document has been prepared under a Standardization Request given to CEN by the European
Commission and the European Free Trade Association.
The CEN/TS 17700 series, Plant biostimulants — Claims, consists of the following parts:
— Part 1: General Principles;
— Part 2: Nutrient use efficiency resulting from the use of a plant biostimulant;
— Part 3: Tolerance to abiotic stress resulting from the use of a plant biostimulant;
— Part 4: Determination of quality traits resulting from the use of a plant biostimulant;
— Part 5: Determination of availability of confined nutrient in the soil or rhizosphere.
Any feedback and questions on this document should be directed to the users’ national standards body.
A complete listing of these bodies can be found on the CEN website.
According to the CEN/CENELEC Internal Regulations, the national standards organisations of the
following countries are bound to announce this Technical Specification: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United
Kingdom.
3

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SIST-TS CEN/TS 17700-5:2023
CEN/TS 17700-5:2022 (E)
Introduction
This document has been developed to provide guidance for a consistent approach to justify the claims
associated with the use of plant biostimulants in agriculture.
The definition of plant biostimulants to be used in the regulation on fertilizing materials is claims-based.
For this reason, demonstrating that a product is indeed a bona fide plant biostimulant depends on a
demonstration of its effect.
The placing of a plant biostimulant on the market should never be considered to guarantee effectiveness
under all conditions, as many factors may influence the performance of a plant biostimulant in the field.
Plant biostimulants used in agriculture can be applied in multiple ways: on soil, on plant, as seed
treatment, etc. This document is applicable to all application types of plant biostimulants in agriculture.
4

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SIST-TS CEN/TS 17700-5:2023
CEN/TS 17700-5:2022 (E)
1 Scope
The claim described in this document concerns the improvement of availability of confined nutrients in
the soil or rhizosphere by a plant biostimulant.
This document is aimed primarily at manufacturers, laboratories, researchers, technical centres,
companies that will put the products on the market, notifying authorities, notified bodies, and market
surveillance authorities.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
CEN/TS 17700-1:2022, Plant biostimulants — Claims Part 1: General Principles
CEN/TS 17724:2022, Plant biostimulants — Terminology
3 Terms and definitions
For the purposes of this document, the terms and definitions given in CEN/TS 17700-1:2022,
CEN/TS 17724:2022 and the following apply.
3.1
available nutrient
element either present in the soil solution or exchangeable on soil colloids
3.2
confined nutrient
element present in the solid and gaseous phase of the soil, except on soil colloids
3.3
improvement of availability of confined nutrients in the soil or rhizosphere
moving soil nutrients from the pool of confined nutrients to the pool of available nutrients
3.4
rhizosphere
volume of soil around living roots that is influenced by root activities
3.5
soil
layer of unconsolidated material consisting of weathered material particles, dead and living organic
matter, air space, and soil solution
3.6
soil solution
liquid phase of the soil and its solutes
3.7
soil colloid
finer size fraction of the soil (clay and organic matter), being also considered as the most chemically active
portion of the soil because of its large surface area and the chemical structure of the materials involved
5

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SIST-TS CEN/TS 17700-5:2023
CEN/TS 17700-5:2022 (E)
4 Terminology of the claims
4.1 Claims
Claimed improvement of availability of confined nutrients shall be completed with one or more
nutrient(s) which is/are concerned by this effect (e.g. nitrogen (N), phosphorus (P), nitrogen and
phosphorus (N and P), zinc (Zn), microelements, …).
Moreover, it is possible to specify in the claim if it is applied to soil or rhizosphere or both.
Some examples of claims which can be mentioned on the label are presented hereunder:
• “Improves availability of microelements confined in the soil and the rhizosphere”,
• “Improves availability of phosphorus in the rhizosphere”,
• “Improves availability of nitrogen in the soil”.
5 Assessment markers to validate the claim
5.1 General considerations for markers measurements
Improvement of the availability of nutrients confined in the soil shall be evaluated by comparing the
concentration or quantity of the available nutrient element (in the soil, soil and rhizosphere or plants) in
the control treatment (without plant biostimulant application) and in the plant biostimulant treatment
(applied plant biostimulant).
Markers shall be determined with the same methods and same equipment on both control treatment and
plant biostimulant treatment.
Markers to evaluate the improvement of the availability of nutrient confined in the soil or rhizosphere,
noted as markers in the text, could be determined on 3 different matrixes (soil, soil and rhizosphere or
plants). For each matrix, markers shall be determined on representative samples.
NOTE Growing media are not considered as soil or soil rhizosphere in this document.
5.2 Soil or rhizosphere markers
5.2.1 Soil sample preparation
Soil or rhizosphere samples should be processed according to EN 16179.
After sampling, the soil or rhizosphere samples should be kept cool and processed as soon as possible
(ISO 18512 may be used for the preservation and storage of soil samples).
Prior to analysis, the soil or rhizosphere samples can be air-dried, or dried in an oven at temperatures
not exceeding 40 °C for at least 72h, or freeze-dried or cool stored (4°C) according to the recommended
methodology for the measured markers.
6

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SIST-TS CEN/TS 17700-5:2023
CEN/TS 17700-5:2022 (E)
5.2.2 Available soil nutrient determination
Available nutrient concentration in the soil or rhizosphere shall be determined after extraction and
analytical determination methods.
Extraction and determination methods
...

SLOVENSKI STANDARD
kSIST-TS FprCEN/TS 17700-5:2021
01-november-2021
[Not translated]
Plant biostimulants - Claims - Part 5: Determination of availability of confined nutrients in
the soil or rhizosphere
Biostimulanzien für die pflanzliche Anwendung - Angaben - Teil 5: Verfügbarkeit von im
Boden und in der Rhizosphäre enthaltenen Nährstoffen
Biostimulants des végétaux - Allégations - Partie 5 : Détermination de la disponibilité des
éléments nutritifs confinés dans le sol ou la rhizosphère
Ta slovenski standard je istoveten z: FprCEN/TS 17700-5
ICS:
65.080 Gnojila Fertilizers
kSIST-TS FprCEN/TS 17700-5:2021 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------
kSIST-TS FprCEN/TS 17700-5:2021

---------------------- Page: 2 ----------------------
kSIST-TS FprCEN/TS 17700-5:2021


FINAL DRAFT
TECHNICAL SPECIFICATION
FprCEN/TS 17700-5
SPÉCIFICATION TECHNIQUE

TECHNISCHE SPEZIFIKATION

September 2021
ICS 65.080
English Version

Plant biostimulants - Claims - Part 5: Determination of
availability of confined nutrients in the soil or rhizosphere
Biostimulants des végétaux - Allégations - Partie 5 : Biostimulanzien für die pflanzliche Anwendung -
Détermination de la disponibilité des éléments nutritifs Angaben - Teil 5: Verfügbarkeit von im Boden und in
confinés dans le sol ou la rhizosphère der Rhizosphäre enthaltenen Nährstoffen


This draft Technical Specification is submitted to CEN members for Vote. It has been drawn up by the Technical Committee
CEN/TC 455.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.

Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of which they are
aware and to provide supporting documentation.

Warning : This document is not a Technical Specification. It is distributed for review and comments. It is subject to change
without notice and shall not be referred to as a Technical Specification.


EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2021 CEN All rights of exploitation in any form and by any means reserved Ref. No. FprCEN/TS 17700-5:2021 E
worldwide for CEN national Members.

---------------------- Page: 3 ----------------------
kSIST-TS FprCEN/TS 17700-5:2021
FprCEN/TS 17700-5:2021 (E)
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Terminology of the claims . 6
4.1 Claims . 6
5 Assessment markers to validate the claim . 6
5.1 General considerations for markers measurements . 6
5.2 Soil or rhizosphere markers . 6
5.2.1 Soil sample preparation . 6
5.2.2 Available soil nutrient determination . 7
5.3 Plant markers . 7
5.3.1 Plant sample preparation . 7
5.3.2 Plant nutrient determination . 7
6 Specifications for the performance of the trials . 8
6.1 General specifications . 8
6.2 Trial design . 8
6.2.1 General . 8
6.2.2 Timetable of measures . 8
Annex A (informative) Methods to measure the nutrient availability in soil or
rhizosphere . 9
Annex B (informative) Digestion and analysis of plant samples .11
B.1 Example of procedure for the digestion of plant samples .11
B.2 Example of methods for plant nutrient analysis .11
Annex C (informative) Triangle of soil Texture.12
Bibliography .13

2

---------------------- Page: 4 ----------------------
kSIST-TS FprCEN/TS 17700-5:2021
FprCEN/TS 17700-5:2021 (E)
European foreword
This document (FprCEN/TS 17700-5:2021) has been prepared by Technical Committee CEN/TC 455
“Plant biostimulants”, the secretariat of which is held by AFNOR.
This document is currently submitted to the Vote on TS.
The CEN/TS 17700 series, Plant biostimulants - Claims, consists of the following parts:
— Part 1: General Principles;
— Part 2: Nutrient use efficiency resulting from the use of a plant biostimulant;
— Part 3: Tolerance to abiotic stress resulting from the use of a plant biostimulant;
— Part 4: Determination of quality traits resulting from the use of a plant biostimulant;
— Part 5: Determination of availability of confined nutrient in the soil or rhizosphere.
3

---------------------- Page: 5 ----------------------
kSIST-TS FprCEN/TS 17700-5:2021
FprCEN/TS 17700-5:2021 (E)
Introduction
This document has been developed to provide guidance for a consistent approach to justify the claims
associated with the use of plant biostimulants in agriculture.
The definition of plant biostimulants to be used in the regulation on fertilizing materials is claims-based.
For this reason, demonstrating that a product is indeed a bona fide plant biostimulant depends on a
demonstration of its effect.
The placing of a plant biostimulant on the market should never be considered to guarantee effectiveness
under all conditions, as many factors may influence the performance of a plant biostimulant in the field.
Plant Biostimulants used in agriculture can be applied in multiple ways: on soil, on plant, as seed
treatment, etc. This document is applicable to all application types of plant biostimulants in agriculture.
4

---------------------- Page: 6 ----------------------
kSIST-TS FprCEN/TS 17700-5:2021
FprCEN/TS 17700-5:2021 (E)
1 Scope
The claim described in this document concerns the improvement of availability of confined nutrients in
the soil or rhizosphere by a plant biostimulant.
This document is aimed primarily at manufacturers, laboratories, researchers, technical centres,
companies that will put the products on market, notifying authorities, notified bodies, and market
surveillance authorities.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
1
CEN/TS 17700-1:— , Plant biostimulants - Claims Part 1: General Principles
2
CEN/TS 17724:— , Plant biostimulants - Terminology
3 Terms and definitions
For the purposes of this document, the terms and definitions given in CEN/TS 17700-1:—,
CEN/TS 17724:— and the following apply.
3.1
available nutrient
element either present in the soil solution or exchangeable on soil colloids
3.2
confined nutrient
element present in the solid and gaseous phases of the soil, excepting soil colloids
3.3
improvement of availability of confined nutrients in the soil or rhizosphere
moving soil nutrients from the pool of confined nutrients to the pool of available nutrients
3.4
rhizosphere
volume of soil around living roots that is influenced by root activities
3.5
soil
layer of unconsolidated material consisting of weathered material particles, dead and living organic
matter, air space, and soil solution
3.6
soil solution
liquid phase of the soil and its solutes

1
Under preparation. Current stage is: FprCEN/TS 17700-1:2021.
2
Under preparation. Current stage is: FprCEN/TS 17724:2021.
5

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kSIST-TS FprCEN/TS 17700-5:2021
FprCEN/TS 17700-5:2021 (E)
3.7
soil colloid
finer size fraction of the soil (clay and organic matter), being also considered as the most chemically active
portion of the soil because of its large surface area and the chemical structure of the materials involved
4 Terminology of the claims
4.1 Claims
Claimed improvement of availability of confined nutrients shall be completed with one or more
nutrient(s) which is/are concerned by this effect (e.g. nitrogen (N), phosphorus (P), nitrogen and
phosphorus (N and P), zinc (Zn), microelements, …).
Moreover, it is possible to specify in the claim if it is applied to soil or rhizosphere or both.
Some examples of claims which can be mentioned on the label are presented hereunder:
• “Improve availability of microelements confined in soil and rhizosphere”,
• “Improve availability of phosphorus in rhizosphere”,
• “Improve availability of nitrogen in soil”.
5 Assessment markers to validate the claim
5.1 General considerations for markers measurements
Improvement of the availability of nutrient confined in the soil shall be evaluated by comparing the
concentration or quantity of the available nutrient element (in the soil, soil and rhizosphere or plants) in
control treatment (without plant biostimulant application) and in plant biostimulant treatment (applied
plant biostimulant).
Markers shall be determined with the same methods and same equipment on both control treatment and
plant biostimulant treatment.
Markers to evaluate the improvement of the availability of nutrient confined in the soil or rhizosphere,
noted as markers in the text, could be determined on 3 different matrixes (soil, soil and rhizosphere or
plants). For each matrix, markers shall be determined on representative samples.
NOTE Growing media are not considered as soil or soil rhizosphere in this document.
5.2 Soil or rhizosphere markers
5.2.1 Soil sample preparation
Soil or rhizosphere samples should be processed according to EN 16179.
After sampling, the soil or rhizosphere samples should be kept cool and processed as soon as possible
(ISO 18512 may be used for the preservation and storage of soil samples).
Prior to analysis, the soil or rhizosphere samples can be air-dried, or dried in an oven at temperatures
not exceeding 40 °C for at least 72h, or freeze-dried or cool stored (4°C) according to the recommended
methodology for the measured markers.
6

---------------------- Page: 8 ----------------------
kSIST-TS FprCEN/TS 17700-5:2021
FprCEN/TS 17700-5:2021 (E)
5.2.2 Available soil nutrient determination
Available nutrient concentration in the soil or rhizosphere shall be determined after extraction and
...

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