SIST EN 17701-2:2025
(Main)Plant biostimulants - Determination of specific elements - Part 2: Determination of total content of Cd, Pb, Ni, As, Cr, Cu and Zn
Plant biostimulants - Determination of specific elements - Part 2: Determination of total content of Cd, Pb, Ni, As, Cr, Cu and Zn
This European Standard specifies a method for the determination of arsenic (As), cadmium (Cd), copper (Cu), chromium (Cr), lead (Pb), nickel (Ni) and zinc (Zn) in plant biostimulant digests using inductively coupled plasma-atomic emission spectrometry (ICP-AES).
This European Standard is also applicable to the blends of fertilizing products where plant biostimulants are the main part of the blend. Otherwise, the Technical Specification for the main part of the blend apply.
This method is applicable to aqua regia digests prepared according to CEN/TS 17701-1. The method may be used for the determination of other elements, provided the user has verified the applicability.
Pflanzen-Biostimulanzien - Bestimmung spezifischer Elemente - Teil 2: Bestimmung des Gesamtgehaltes an Cd, Pb, Ni, As, Cr, Cu und Zn
Dieses Dokument legt ein Verfahren zur Bestimmung des Gehalts an Arsen (As), Cadmium (Cd), Kupfer (Cu), Chrom (Cr), Blei (Pb), Nickel (Ni) und Zink (Zn) in Pflanzen-Biostimulanzien mit Atomemissions¬spektrometrie mit induktiv gekoppeltem Plasma (ICP AES) nach Königswasseraufschluss fest.
Dieses Dokument ist für Düngeproduktmischungen anwendbar, die zur PFC 7 gehören und bei denen das in der Mischung enthaltene EU Düngeprodukt Pflanzen-Biostimulans den größten Massenanteil in % in der Mischung ausmacht. Im Fall von gleich großen Anteilen kann der Anwender entweder die vorliegende Norm oder die für den/die anderen Bestandteil(e) anwendbare(n) Norm(en) verwenden.
Das Verfahren ist für Königswasseraufschlüsse anwendbar, die nach EN 17701 1:—1 hergestellt wurden. Das Verfahren kann zur Bestimmung anderer Elemente angewendet werden, vorausgesetzt der Anwender hat die Anwendbarkeit überprüft.
ANMERKUNG Alternativ kann die Massenspektrometrie mit induktiv gekoppeltem Plasma (ICP MS) für die Bestimmung der Elemente in den Königswasser-Aufschlüssen genutzt werden, sofern der Anwender nachweist, dass das Verfahren zu den gleichen Ergebnissen führt.
Biostimulants des végétaux - Dosage des éléments spécifiques - Partie 2 : Détermination de la teneur totale en Cd, Pb, Ni, As, Cr, Cu et Zn
Le présent document spécifie une méthode pour le dosage de l’arsenic (As), du cadmium (Cd), du cuivre (Cu), du chrome (Cr), du plomb (Pb), du nickel (Ni) et du zinc (Zn) dans les digestats de biostimulants des végétaux à l’eau régale par spectrométrie d’émission atomique par un plasma à couplage inductif (ICP-AES).
Le présent document est applicable aux mélanges de fertilisants appartenant à la catégorie PFC 7, dans lesquels le fertilisant UE biostimulant des végétaux contenu dans le mélange représente le plus fort % en masse du mélange. Dans le cas où les proportions sont égales, l’utilisateur peut appliquer la présente norme ou la ou les normes applicables aux autres produits constituants.
Cette méthode est applicable aux digestats à l’eau régale préparés conformément à l’EN 17701 1:—1. La méthode peut également être utilisée pour le dosage d’autres éléments, à condition que l’utilisateur ait vérifié son applicabilité.
NOTE Il est également possible d’utiliser la spectrométrie de masse avec plasma à couplage inductif (ICP-MS) pour le dosage des éléments dans les digestats à l’eau régale, si l’utilisateur prouve que la méthode conduit aux mêmes résultats.
Rastlinski biostimulanti - Določanje specifičnih elementov - 2. del: Določanje celotnega Cd, Pb, Ni, As, Cr, Cu in Zn
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-februar-2025
Nadomešča:
SIST-TS CEN/TS 17701-2:2023
Rastlinski biostimulanti - Določanje specifičnih elementov - 2. del: Določanje
celotnega Cd, Pb, Ni, As, Cr, Cu in Zn
Plant biostimulants - Determination of specific elements - Part 2: Determination of total
content of Cd, Pb, Ni, As, Cr, Cu and Zn
Pflanzen-Biostimulanzien - Bestimmung spezifischer Elemente - Teil 2: Bestimmung des
Gesamtgehaltes an Cd, Pb, Ni, As, Cr, Cu und Zn
Biostimulants des végétaux - Dosage des éléments spécifiques - Partie 2 :
Détermination de la teneur totale en Cd, Pb, Ni, As, Cr, Cu et Zn
Ta slovenski standard je istoveten z: EN 17701-2:2024
ICS:
65.080 Gnojila Fertilizers
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN 17701-2
EUROPEAN STANDARD
NORME EUROPÉENNE
November 2024
EUROPÄISCHE NORM
ICS 65.080 Supersedes CEN/TS 17701-2:2022
English Version
Plant biostimulants - Determination of specific elements -
Part 2: Determination of total content of Cd, Pb, Ni, As, Cr,
Cu and Zn
Biostimulants des végétaux - Dosage des éléments Pflanzen-Biostimulanzien - Bestimmung spezifischer
spécifiques - Partie 2 : Détermination de la teneur Elemente - Teil 2: Bestimmung des Gesamtgehaltes an
totale en Cd, Pb, Ni, As, Cr, Cu et Zn Cd, Pb, Ni, As, Cr, Cu und Zn
This European Standard was approved by CEN on 26 August 2024.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2024 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 17701-2:2024 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle . 6
5 Interferences . 6
5.1 General. 6
5.2 Spectral interferences . 6
5.3 Transport interferences . 7
5.4 Excitation interferences . 7
5.5 Chemical interferences . 7
5.6 Memory interferences . 7
6 Reagents . 7
7 Apparatus . 8
8 Procedure . 8
8.1 Preparation of test sample solution and blank test solution . 8
8.2 Preparation of the calibration solutions . 9
8.3 Measurement . 9
8.3.1 Instrument conditions . 9
8.3.2 Optimization of the instrument conditions . 9
8.3.3 Interferences . 9
8.3.4 Measurement . 10
8.3.5 Matrix effects . 10
8.3.6 Spiking . 10
9 Calculation and expression of the results . 12
10 Test report . 12
Annex A (informative) Results of the inter-laboratory study . 13
A.1 Inter-laboratory tests . 13
A.2 Statistical results for the determination of the total contents of the elements. 13
Annex ZA (informative) Relationship of this European Standard and the essential
requirements of Regulation (EU) 2019/1009 making available on the market of EU
fertilising products aimed to be covered . 21
Bibliography . 22
European foreword
This document (EN 17701-2:2024) has been prepared by Technical Committee CEN/TC 455 “Plant
biostimulants”, the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by May 2025, and conflicting national standards shall be
withdrawn at the latest by May 2025.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes CEN/TS 17701-2:2022.
CEN/TS 17701-2:2022:
— scope text regarding blends has been changed;
— Annex A “Results of the inter-laboratory study” has been added;
— Annex ZA has been added.
This document has been prepared under a standardization request addressed to CEN by the European
Commission. The Standing Committee of the EFTA States subsequently approves these requests for its
Member States.
For the relationship with EU Legislation, see informative Annex ZA, which is an integral part of this
document.
EN 17701 series, Plant biostimulants — Determination of specific elements, consists of the following parts:
— Part 1: Digestion by aqua regia for subsequent determination of elements;
— Part 2: Determination of total content of Cd, Pb, Ni, As, Cr, Cu and Zn;
— Part 3: Determination of mercury.
Any feedback and questions on this document should be directed to the users’ national standards body.
A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organisations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia,
Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland,
Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of North
Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the United
Kingdom.
Introduction
The European Committee for Standardization (CEN) was requested by the European Commission (EC) to
draft European Standards or European Standardization deliverables to support the implementation of
Regulation (EU) 2019/1009 of 5 June 2019 [1] laying down rules on the making available on the market
of EU fertilising products (“FPR” or “Fertilising Products Regulation”).
This standardization request, presented as SR M/564 and relevant amendments, also contributes to the
Communication on “Innovating for Sustainable Growth: A Bio economy for Europe”. The interest in plant
biostimulants has increased significantly in Europe as a valuable tool to use in agriculture.
Standardization was identified as having an important role in order to promote the use of biostimulants.
The work of CEN/TC 455 seeks to improve the reliability of the supply chain, thereby improving the
confidence of farmers, industry, and consumers in biostimulants, and will promote and support
commercialisation of the European biostimulant industry
This document concerns the analytical measurement step. Aqua regia digestion followed by inductively
coupled plasma atomic emission spectrometry (ICP-AES) is widely used for determination of many
elements, for example, a multi-matrix standard for aqua regia extraction of soils, sludges and biowaste
[2] and soil [3]. A similar procedure was applied for determination of aqua regia extractable contents of
arsenic, mercury, cadmium, chromium, nickel and lead in fertilizers and liming materials [4, 5] and for
determination of micronutrients in mineral fertilizers [6]. Inductively coupled plasma atomic emission
spectrometry (ICP-AES) is nowadays widely used and a well-established method in many laboratories.
The inter-laboratory study reflects the final statistical characteristics of the method for the determination
of the specific elements in aqua regia digests including both, the digestion and the measurement steps.
WARNING — Persons using this document should be familiar with usual laboratory practice. This
document does not purport to address all of the safety issues, if any, associated with its use. It is the
responsibility of the user to establish appropriate health and safety practices and to ensure compliance
with any national regulatory conditions.
IMPORTANT — It is absolutely essential that tests conducted according to this document are carried out
by suitably trained staff.
1 Scope
This document specifies a method for the determination of total contents of arsenic (As), cadmium (Cd),
copper (Cu), chromium (Cr), lead (Pb), nickel (Ni) and zinc (Zn) in aqua regia plant biostimulant digests
using inductively coupled plasma-atomic emission spectrometry (ICP-AES).
This document is applicable to the blends of fertilizing products where a blend is a mix of at least two of
the following component EU fertilising products categories: Fertilizers, Liming Materials, Soil Improvers,
Growing Media, Inhibitors, Plant Biostimulants, and where the following category Plant Biostimulants is
the highest % in the blend by mass or volume, or in the case of liquid form by dry mass. If Plant
Biostimulants is not the highest % in the blend, the European Standard for the highest % of the blend
applies. In case a blend of fertilizing products is composed of components in equal quantity or in case the
component EU fertilising products used for the blend have identical formulations , the user decides
which standard to apply.
This method is applicable to aqua regia digests prepared according to EN 17701-1:2024. The method can
be used for the determination of other elements, provided the user has verified the applicability.
NOTE Alternatively, inductively coupled plasma mass spectrometry (ICP-MS) can be used for the
determination of the elements in the aqua regia digests if the user proves that the method gives the same results.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
EN 17701-1:2024, Plant biostimulants —
...
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