SIST EN 17714:2025
(Main)Plant biostimulants - Determination of microorganisms' concentration
Plant biostimulants - Determination of microorganisms' concentration
This document specifies general rules to determine the concentration of microorganisms present in plant biostimulants.
The method is applicable to microbial plant biostimulants for verifying that the concentration of microorganisms does not exceed the respective limits outlined in the EU Regulation on Fertilising Products [1].
This horizontal method might not be appropriate in every detail for certain products. In this case, it is necessary to refer to the methodology of specific determination and quantification of the microorganisms.
Pflanzen-Biostimulanzien - Bestimmung der Konzentration von Mikroorganismen
Dieses Dokument legt allgemeine Regeln für die Bestimmung der Konzentration von Mikroorganismen in Pflanzen-Biostimulanzien fest.
Das Verfahren ist auf mikrobielle Pflanzen-Biostimulanzien anwendbar, und zwar, um zu verifizieren, dass die Konzentration von Mikroorganismen die entsprechenden Grenzwerte der EU Düngeprodukteverordnung [1] nicht überschreitet.
Dieses horizontale Verfahren ist für bestimmte Produkte nicht unbedingt bis ins Detail geeignet. In diesem Fall ist es notwendig, auf die Methodik der spezifischen Bestimmung und Quantifizierung der Mikro-organismen zurückzugreifen.
Biostimulants des végétaux - Détermination de la concentration en microorganismes
Le présent document spécifie les règles générales pour déterminer la concentration en microorganismes présents dans les biostimulants des végétaux.
La méthode est applicable aux biostimulants des végétaux microbiens pour vérifier que la concentration en microorganismes ne dépasse pas les limites correspondantes indiquées dans le Règlement UE sur les fertilisants [1].
Cette méthode horizontale pourrait ne pas être appropriée dans chaque détail pour certains produits. Dans ce cas, il est nécessaire de se référer à la méthodologie de détermination et de quantification spécifique des microorganismes.
Rastlinski biostimulanti - Določanje koncentracije mikroorganizmov
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-februar-2025
Nadomešča:
SIST-TS CEN/TS 17714:2023
Rastlinski biostimulanti - Določanje koncentracije mikroorganizmov
Plant biostimulants - Determination of microorganisms' concentration
Pflanzen-Biostimulanzien - Bestimmung der Konzentration von Mikroorganismen
Biostimulants des végétaux - Détermination de la concentration en microorganismes
Ta slovenski standard je istoveten z: EN 17714:2024
ICS:
65.080 Gnojila Fertilizers
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN 17714
EUROPEAN STANDARD
NORME EUROPÉENNE
November 2024
EUROPÄISCHE NORM
ICS 65.080 Supersedes CEN/TS 17714:2022
English Version
Plant biostimulants - Determination of microorganisms'
concentration
Biostimulants des végétaux - Détermination de la Pflanzen-Biostimulanzien - Bestimmung der
concentration en microorganismes Konzentration von Mikroorganismen
This European Standard was approved by CEN on 26 August 2024.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2024 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 17714:2024 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle of the method . 6
5 Sampling . 7
6 Preparation of sample for microbial analysis . 7
7 Method for the enumeration of microorganisms . 7
7.1 General. 7
7.2 Enumeration using solid media . 7
7.2.1 General. 7
7.2.2 Number of Petri dishes per dilution . 7
7.2.3 Pour plate techniques . 8
7.2.4 Surface inoculation . 8
7.2.5 Incubation. 9
7.2.6 Calculation and expression of results obtained with solid media . 9
7.3 Enumeration and quantification using a liquid medium . 15
7.3.1 Principle . 15
7.3.2 Inoculation . 16
7.3.3 Choice of inoculation system . 16
8 Expression of results . 17
9 Test report . 18
10 Quality assurance . 18
Annex ZA (informative) Relationship of this European Standard and the essential
requirements of Regulation (EU) 2019/1009 making available on the market of EU
fertilising products aimed to be covered . 19
Bibliography . 20
European foreword
This document (EN 17714:2024) has been prepared by Technical Committee CEN/TC 455 “Plant
biostimulants”, the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by May 2025, and conflicting national standards shall be
withdrawn at the latest by May 2025.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes CEN/TS 17714:2022.
— the European foreword and the Introduction have been updated;
— normative references have been updated;
— Annex ZA has been added.
This document has been prepared under a standardization request addressed to CEN by the European
Commission. The Standing Committee of the EFTA States subsequently approves these requests for its
Member States.
For the relationship with EU Legislation, see informative Annex ZA, which is an integral part of this
document.
Any feedback and questions on this document should be directed to the users’ national standards body.
A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organisations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia,
Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland,
Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of North
Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the United
Kingdom.
Introduction
The European Committee for Standardization (CEN) was requested by the European Commission (EC) to
draft European Standards or European Standardization deliverables to support the implementation of
Regulation (EU) 2019/1009 of 5 June 2019 [1] laying down rules on the making available on the market
of EU fertilising products (“FPR” or “Fertilising Products Regulation”).
This standardization request, presented as SR M/564 and relevant amendments, also contributes to the
Communication on “Innovating for Sustainable Growth: A Bio economy for Europe”. The interest in plant
biostimulants has increased significantly in Europe as a valuable tool to use in agriculture.
Standardization was identified as having an important role in order to promote the use of biostimulants.
The work of CEN/TC 455 seeks to improve the reliability of the supply chain, thereby improving the
confidence of farmers, industry, and consumers in biostimulants, and will promote and support
commercialisation of the European biostimulant industry.
WARNING — Persons using this document should be familiar with normal laboratory practice. This
document does not purport to address all of the safety problems, if any, associated with its use. It is the
responsibility of the user to establish appropriate safety and health practices and to ensure compliance
with any national regulatory conditions.
IMPORTANT — It is absolutely essential that tests conducted in accordance with this document be
carried out by suitably trained staff.
1 Scope
This document specifies the general rules to determine the concentration of microorganisms present in
plant biostimulants expressed as the number of active units per volume or weight, or in any other manner
that is relevant to the microorganism, e.g. colony forming units per gram (cfu/g).
This document is applicable to the blends of fertilizing products where a blend is a mix of at least two of
the following component EU fertilising products categories: Fertilizers, Liming Materials, Soil Improvers,
Growing Media, Plant Biostimulants, and where the following category Plant Biostimulants is the highest
% in the blend by mass or volume, or in the case of liquid form by dry mass. If Plant Biostimulants is not
the highest % in the blend, the European Standard for the highest % of the blend applies. In case a blend
of fertilizing products is composed of components in equal quantity or in case the component EU
fertilising products used for the blend have identical formulations , the user decides which standard to
apply.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
EN 17708:2024, Plant biostimulants — Preparation of sample for microbial analysis
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https://www.iso.org/obp;
— IEC Electropedia: available at https://www.electropedia.org/
3.1
plant biostimulant
product stimulating plant nutrition processes independently of the product’s nutrient content with the
sole aim of improving the nutrient use efficiency, the tolerance to abiotic stress, the quality traits of the
plant or the plant rhizosphere or the availability of confined nutrient in soil or rhizosphere
[SOURCE: EN 17724:2024, 3.1.1.8 [3], modified – editorial changes]
3.2
microorganism
any microbiological entity, including lower fungi, bacteria and viruses, cellular or non-cellular, capable of
replication or of transferring genetic material, including dead or empty cells, microorganisms and non-
harmful elements of the media on which they were produced
[SOURCE: EN 17724:2024, 3.2.2 [3], modified – “including dead or empty cells, microorganisms and non-
harmful elements of the media on which they were produced” added]
An example of such a blend is a product with 2 claimed functions consisting of a non-microbial plant biostimulant
and an organic fertilizer composed of 1kg/kg of plant biostimulant from seaweed.
3.3
colony
localized visible accumulation of microbial mass (such as prokaryotes, bacteria, micromycetes, yeasts and
fungi) or organisms (such as Dreissena species) developed on or in a solid nutrient medium from a viable
particle or organism
Note 1 to entry: Frequently, microcolonies from nearby viable particles, before becoming visible, fuse into one
macrocolony. The number of visible colonies is, therefore, usually an underestimate of the number of viable
particles.
[SOURCE: ISO 6107:2021, 3.119 [4]]
3.4
product
portion of an identified plant biostimulant received in the laboratory for testing
3.5
laboratory sample
final sample intended for laboratory testing and in relation to microbiological testing, each separate
segment sample intended for laboratory testing
3.6
initial suspension
suspension (or solution) of the laboratory sample (3.5) in a defined volume of an appropriate diluent
3.7
Unit Potential Mycorrhizal
UPM
unit of counting for mycorrhiza
where
U is unit, spore or propagule of any type able to initiate mycorrhiza formation in a host plant’s root;
P is potential, since the development of the symbiosis depend on different factors (soil, plant,
agriculture practises, competition with other soil borne microorganisms, etc.);
M is mycorrhizal, since the inoculum is able to synthesize new mycorrhizae in associatio
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