SIST EN 1104:2005

Paper and board intended to come into contact with foodstuffs - Determination of the transfer of antimicrobial constituents

This European Standard specifies a method for the determination of transfer of antimicrobial constituents from paper and board materials and articles intended for food contact.

Papier und Pappe vorgesehen für den Kontakt mit Lebensmitteln - Bestimmung des Übergangs antimikrobieller Bestandteile

Diese Europäische Norm legt ein Verfahren zur Bestimmung der Übertragung antimikrobieller Bestandteile
von Materialien und Artikeln, bestehend aus Papier, Karton und Pappe fest, die für den Kontakt mit
Lebensmitteln vorgesehen sind.

Papier et carton destinés a entrer en contact avec des denrées alimentaires - Détermination du transfer des constituants antimicrobiens

Cette Norme européenne prescrit une méthode de détermination du transfert des constituants antimicrobiens
des papiers, cartons et articles pour contact alimentaire.

Papir, karton in lepenka v neposrednem stiku z živili – Določanje izločanja antimikrobnih snovi

General Information

• Status: Withdrawn
• Publication Date: 30-Nov-2005
• Withdrawal Date: 05-Dec-2018
• ICS: 67.250 - Materials and articles in contact with foodstuffs
• ICS: 85.060 - Paper and board
• Technical Committee: VPK - Pulp, paper, board and products
• Current Stage: 9900 - Withdrawal (Adopted Project)
• Start Date: 06-Dec-2018
• Due Date: 29-Dec-2018
• Completion Date: 06-Dec-2018
• Ref Project: EN 1104:2005 - Paper and board intended to come into contact with foodstuffs - Determination of the transfer of antimicrobial constituents

Relations

• Replaced By: SIST EN 1104:2019 - Paper and board intended to come into contact with foodstuffs - Determination of the transfer of antimicrobial constituents
• Effective Date: 01-Jan-2019
• Replaced By: SIST EN 1104:2019 - Paper and board intended to come into contact with foodstuffs - Determination of the transfer of antimicrobial constituents
• Effective Date: 08-Jun-2022
• Replaces: SIST EN 1104:1997 - Paper and board intended to come into contact with foodstuff - Determination of transfer of antimicrobic constituents
• Effective Date: 01-Dec-2005

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN 1104:2005
01-december-2005
1DGRPHãþD
SIST EN 1104:1997
3DSLUNDUWRQLQOHSHQNDYQHSRVUHGQHPVWLNX]åLYLOL±'RORþDQMHL]ORþDQMD
DQWLPLNUREQLKVQRYL
Paper and board intended to come into contact with foodstuffs - Determination of the
transfer of antimicrobial constituents
Papier und Pappe vorgesehen für den Kontakt mit Lebensmitteln - Bestimmung des
Übergangs antimikrobieller Bestandteile
Papier et carton destinés a entrer en contact avec des denrées alimentaires -
Détermination du transfer des constituants antimicrobiens
Ta slovenski standard je istoveten z: EN 1104:2005
ICS:
67.250 Materiali in predmeti v stiku z Materials and articles in
živili contact with foodstuffs
85.060 Papir, karton in lepenka Paper and board
SIST EN 1104:2005 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN 1104:2005

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SIST EN 1104:2005
EUROPEAN STANDARD
EN 1104
NORME EUROPÉENNE
EUROPÄISCHE NORM
August 2005
ICS 67.250; 85.060 Supersedes EN 1104:1995
English Version
Paper and board intended to come into contact with foodstuffs -
Determination of the transfer of antimicrobial constituents
Papier et carton destinés à entrer en contact avec des Papier und Pappe vorgesehen für den Kontakt mit
denrées alimentaires - Détermination du transfer des Lebensmitteln - Bestimmung des Übergangs
constituants antimicrobiens antimikrobieller Bestandteile
This European Standard was approved by CEN on 27 June 2005.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the Central Secretariat or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the official
versions.
CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,
Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia,
Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36  B-1050 Brussels
© 2005 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 1104:2005: E
worldwide for CEN national Members.

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SIST EN 1104:2005
EN 1104:2005 (E)
Contents page
Foreword .3
1 Scope.4
2 Normative references.4
3 Terms and definitions.4
4 Principle.4
5 Apparatus.4
5.1 Punch iron,.4
5.2 Pressing device,.4
5.3 Zone reading device, .4
5.4 Ordinary microbiological laboratory apparatus .5
6 Reagents.5
6.1 Water,.5
6.2 Non ionic wetting agent,.5
6.3 Nutrient medium for Bacillus subtilis.5
6.4 Sabouraud modified mould nutrient medium for Aspergillus niger .6
6.5 Nutrient medium for inhibition test with Bacillus subtilis .6
6.6 Salt peptone solution.6
6.7 Test micro-organisms.7
6.7.1 General.7
6.7.2 Preparation of inoculating spore suspension of Bacillus subtilis .7
6.7.3 Preparation of inoculating spore suspension of Aspergillus niger .8
6.7.4 Concentrations of spores for inhibition test.8
6.8 Positive controls.8
6.8.1 Penicillin, G 0,03 units .8
6.8.2 Isothiazolinon-solution.8
7 Sampling and preparation of test pieces .8
8 Procedure.9
8.1 Sterilisation.9
8.2 Preparation of plates.9
8.2.1 General.9
8.2.2 Bacillus subtilis.9
8.2.3 Aspergillus niger.9
8.3 Incubation.9
9 Evaluation.10
10 Test report.10

2

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SIST EN 1104:2005
EN 1104:2005 (E)
Foreword
This European Standard (EN 1104:2005) has been prepared by Technical Committee CEN/TC 172 “Pulp,
paper and board”, the secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by February 2006, and conflicting national standards shall be withdrawn
at the latest by February 2006.
This European Standard supersedes EN 1104:1995.
With regard to EN 1104:1995 the following changes have been made:
a) description of the preparation of some reagents were partly modified;
b) more detailed description of the procedure was formulated;
c) editorial updating.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Cyprus, Czech Republic,
Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia, Slovenia, Spain, Sweden, Switzerland
and United Kingdom.
3

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SIST EN 1104:2005
EN 1104:2005 (E)
1 Scope
This European Standard specifies a method for the determination of transfer of antimicrobial constituents from
paper and board materials and articles intended for food contact.
2 Normative references
The following referenced documents are indispensable for the application of this European Standard. For
dated references, only the edition cited applies. For undated references, the latest edition of the referenced
document (including any amendments) applies.
EN ISO 186, Paper and board — Sampling to determine average quality (ISO 186:2002)
3 Terms and definitions
For the purposes of this European Standard, the following term and definition applies.
3.1
inhibition zone
zone formed around the test pieces, placed on nutrient medium, which has been seeded with a pre-selected
test organism that releases water-soluble antimicrobial constituents.
4 Principle
A prepared nutrient medium is mixed with an appropriate inoculum and poured into Petri dishes. The test
pieces are placed on the semi solid nutrient medium and then incubated. When incubation is terminated, the
existence of an inhibition zone is an indicator of the release of antimicrobial constituents.
The test is performed with a bacterium, Bacillus subtilis, and with a fungus, Aspergillus niger.
NOTE The result is based on a visual inspection.
5 Apparatus
5.1 Punch iron,
d = 10 mm to 15 mm, sterilizable.
5.2 Pressing device,
suitable for pressing the test pieces on the agar plate (e.g. Drygalski spatula).
5.3 Zone reading device,
to measure the diameter of inhibition.
NOTE Measuring the diameter of the inhibition zone is not compulsory.
4

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SIST EN 1104:2005
EN 1104:2005 (E)
5.4 Ordinary microbiological laboratory apparatus
6 Reagents
6.1 Water,
freshly distilled or water purified by ion exchange and freshly boiled (deionised water).
6.2 Non ionic wetting agent,
for example polyoxyethylenesorbitane monooleate.
6.3 Nutrient medium for Bacillus subtilis
A typical formulation of the nutrient medium is:
 beef extract 3,0 g;
 tryptone (peptone of casein) 5,0 g;
 sodium chloride, pure 5,0 g;
 agar-agar 12,0 g
 water 1000,0 ml.
NOTE The addition of 10 mg/l MnSO to the nutrient medium for Bacillus subtilis (6.7.1) will support the formation of
4
spores.
Prepare the nutrient medium as follows:
Dissolve the components, or a ready-made medium of a comparable composition, in water by boiling.
The pH of the ready prepared nutrient medium shall be (7,2 ± 0,2) referred at a temperature of 45 °C.
Adjust the pH to (7,2 ± 0,2) as required either with NaOH approx. 0,01 M or HCl approx. 0,01 M.
Separate the nutrient medium into two parts.
Dispens
...