Microbiology of food and animal feed - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 6: Specific rules for the preparation of samples taken at the primary production stage (ISO 6887-6:2013)

This part of ISO 6887 specifies rules for the preparation of samples taken at all stages from the farm to the slaughterhouse and their suspension for microbiological examination when the samples require different preparation from the methods described in ISO 6887-1. ISO 6887-1 defines the general rules for the preparation of the initial suspension and decimal dilutions for microbiological examination. This part of ISO 6887 excludes the preparation of samples for both enumeration and detection test methods where preparation details are specified in the relevant International Standards. This part of ISO 6887 is applicable to various samples taken from the hatchery, the farm, from the vehicle or the animals during transportation, or from animals or their carcasses in the slaughterhouse, to indicate the microbiological status of the animals in relation to zoonotic agents. This part of ISO 6887 does not apply to samples taken to assess the hygiene of meat. These are covered by ISO 6887-2. This part of ISO 6887 does not consider samples taken from the aquatic environment (marine or freshwater) at the primary production stage. These are covered by ISO 6887-3.

Mikrobiologie von Lebensmitteln und Futtermitteln - Vorbereitung von Untersuchungsproben und Herstellung von Erstverdünnungen und von Dezimalverdünnungen für mikrobiologische Untersuchungen - Teil 6: Spezifische Regeln für die Vorbereitung von Proben aus der Primärproduktion (ISO 6887-6:2013)

Die Primärproduktion umfasst alle Stufen vom Bauernhof bis zum Schlachthof. Im Anwendungsbereich enthalten sind Proben, die zum Nachweis und/oder zur Zählung von Mikroorganismen bei lebenden Tieren oder aus der Umgebung entnommen werden. Ausgeschlossen sind Proben, die zur Beurteilung der Hygiene von Fleisch entnommen werden.  
Dieser Teil von ISO 6887 ist bei verschiedenartigen Proben anwendbar, wie Proben, die in Brütereien, auf Bauernhöfen, von Fahrzeugen oder von Tieren während des Transports und von Tieren oder deren Schlachtkörpern in Schlacht¬höfen entnommen werden, um den mikrobiologischen Status der Tiere in Bezug auf zoonotische Krankheits¬erreger aufzuzeigen.

Microbiologie des aliments - Préparation des échantillons, de la suspension mère et des dilutions décimales en vue de l'examen microbiologique - Partie 6: Règles spécifiques pour la préparation des échantillons prélevés au stade de production primaire (ISO 6887-6:2013)

L'ISO 6887-6:2013 spécifie des règles pour la préparation d'échantillons prélevés à toutes les étapes depuis l'élevage jusqu'à l'abattoir et leur suspension pour examen biologique lorsque les échantillons nécessitent une méthode de préparation différente de celles décrites dans l'ISO 6887-1.
L'ISO 6887-6:2013 n'inclut pas la préparation d'échantillons destinés à des méthodes de recherche et de dénombrement dont les détails concernant la préparation sont spécifiés dans les Normes internationales appropriées.
L'ISO 6887-6:2013 est applicable à différents échantillons prélevés dans un couvoir, un élevage, un véhicule ou sur les animaux pendant le transport, ou sur les animaux ou leur carcasse à l'abattoir, afin d'indiquer le statut microbiologique des animaux par rapport à des agents zoonotiques. Elle ne s'applique pas aux échantillons prélevés pour évaluer l'hygiène de la viande.

Mikrobiologija živil in krme - Priprava vzorcev za preskušanje ter osnovnih in decimalnih razredčin za mikrobiološko preiskavo - 6. del: Posebna pravila za pripravo vzorcev iz primarne proizvodne faze (ISO 6887-6:2013)

Ta del standarda ISO 6887 določa pravila za pripravo vzorcev, ki se jemljejo na vseh stopnjah, tj. od kmetije do klavnice, in njihove razredčine za mikrobiološko preiskavo, kadar se za vzorce zahteva drugačna priprava od metod iz standarda ISO 6887-1. Standard ISO 6887-1 določa splošna pravila za pripravo osnovnih in decimalnih razredčin za mikrobiološko preiskavo. Ta del standarda ISO 6887 ne zajema priprave vzorcev za preskusni metodi za ugotavljanje števila in odkrivanje, kadar so podrobnosti glede priprave opredeljene v ustreznih mednarodnih standardih. Ta del standarda ISO 6887 se uporablja za različne vzorce, odvzete v valilnici, na kmetiji, v vozilu ali od živali med prevozom ali od živali ali njihovih trupov v klavnici, da se ugotovi mikrobiološki status živali v zvezi s povzročitelji zoonoze. Ta del standarda ISO 6887 se ne uporablja za vzorce, ki se jemljejo za oceno higiene mesa. Ti vzorci so obravnavani v standardu EN 6887-2. Ta del standarda ISO 6887 ne obravnava vzorcev iz vodnega okolja (morskega ali sladkovodnega) v primarni proizvodni fazi. Ti vzorci so obravnavani v standardu EN 6887-3.

General Information

Status
Published
Public Enquiry End Date
14-Mar-2011
Publication Date
21-May-2013
Current Stage
6060 - National Implementation/Publication (Adopted Project)
Start Date
22-Apr-2013
Due Date
27-Jun-2013
Completion Date
22-May-2013

Buy Standard

Standard
EN ISO 6887-6:2013
English language
16 pages
sale 10% off
Preview
sale 10% off
Preview
e-Library read for
1 day

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN ISO 6887-6:2013
01-junij-2013
0LNURELRORJLMDåLYLOLQNUPH3ULSUDYDY]RUFHY]DSUHVNXãDQMHWHURVQRYQLKLQ
GHFLPDOQLKUD]UHGþLQ]DPLNURELRORãNRSUHLVNDYRGHO3RVHEQDSUDYLOD]D
SULSUDYRY]RUFHYL]SULPDUQHSURL]YRGQHID]H ,62
Microbiology of food and animal feed - Preparation of test samples, initial suspension
and decimal dilutions for microbiological examination - Part 6: Specific rules for the
preparation of samples taken at the primary production stage (ISO 6887-6:2013)
Mikrobiologie von Lebensmitteln und Futtermitteln - Vorbereitung von
Untersuchungsproben und Herstellung von Erstverdünnungen und von
Dezimalverdünnungen für mikrobiologische Untersuchungen - Teil 6: Spezifische Regeln
für die Vorbereitung von Proben aus der Primärproduktion (ISO 6887-6:2013)
Microbiologie des aliments - Préparation des échantillons, de la suspension mère et des
dilutions décimales en vue de l'examen microbiologique - Partie 6: Règles spécifiques
pour la préparation des échantillons prélevés au stade de production primaire (ISO 6887-
6:2013)
Ta slovenski standard je istoveten z: EN ISO 6887-6:2013
ICS:
07.100.30 Mikrobiologija živil Food microbiology
SIST EN ISO 6887-6:2013 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------

SIST EN ISO 6887-6:2013

---------------------- Page: 2 ----------------------

SIST EN ISO 6887-6:2013


EUROPEAN STANDARD
EN ISO 6887-6

NORME EUROPÉENNE

EUROPÄISCHE NORM
March 2013
ICS 07.100.30
English Version
Microbiology of food and animal feed - Preparation of test
samples, initial suspension and decimal dilutions for
microbiological examination - Part 6: Specific rules for the
preparation of samples taken at the primary production stage
(ISO 6887-6:2013)
Microbiologie des aliments - Préparation des échantillons, Mikrobiologie von Lebensmitteln und Futtermitteln -
de la suspension mère et des dilutions décimales en vue Vorbereitung von Untersuchungsproben und Herstellung
de l'examen microbiologique - Partie 6: Règles spécifiques von Erstverdünnungen und von Dezimalverdünnungen für
pour la préparation des échantillons prélevés au stade de mikrobiologische Untersuchungen - Teil 6: Spezifische
production primaire (ISO 6887-6:2013) Regeln für die Vorbereitung von Proben aus der
Primärproduktion (ISO 6887-6:2013)
This European Standard was approved by CEN on 16 February 2013.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same
status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United
Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2013 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 6887-6:2013: E
worldwide for CEN national Members.

---------------------- Page: 3 ----------------------

SIST EN ISO 6887-6:2013
EN ISO 6887-6:2013 (E)
Contents Page
Foreword . 3

2

---------------------- Page: 4 ----------------------

SIST EN ISO 6887-6:2013
EN ISO 6887-6:2013 (E)
Foreword
This document (EN ISO 6887-6:2013) has been prepared by Technical Committee CEN/TC 275 “Food
analysis - Horizontal methods”, the secretariat of which is held by DIN, in collaboration with Technical
Committee ISO/TC 34 "Food products".
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by September 2013, and conflicting national standards shall be
withdrawn at the latest by September 2013.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech
Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece,
Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom.
3

---------------------- Page: 5 ----------------------

SIST EN ISO 6887-6:2013

---------------------- Page: 6 ----------------------

SIST EN ISO 6887-6:2013
INTERNATIONAL ISO
STANDARD 6887-6
First edition
2013-03-01
Microbiology of food and animal
feed — Preparation of test samples,
initial suspension and decimal
dilutions for microbiological
examination —
Part 6:
Specific rules for the preparation
of samples taken at the primary
production stage
Microbiologie des aliments — Préparation des échantillons, de
la suspension mère et des dilutions décimales en vue de l’examen
microbiologique —
Partie 6: Règles spécifiques pour la préparation des échantillons
prélevés au stade de production primaire
Reference number
ISO 6887-6:2013(E)
©
ISO 2013

---------------------- Page: 7 ----------------------

SIST EN ISO 6887-6:2013
ISO 6887-6:2013(E)

COPYRIGHT PROTECTED DOCUMENT
© ISO 2013
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior
written permission. Permission can be requested from either ISO at the address below or ISO’s member body in the country of
the requester.
ISO copyright office
Case postale 56 • CH-1211 Geneva 20
Tel. + 41 22 749 01 11
Fax + 41 22 749 09 47
E-mail copyright@iso.org
Web www.iso.org
Published in Switzerland
ii © ISO 2013 – All rights reserved

---------------------- Page: 8 ----------------------

SIST EN ISO 6887-6:2013
ISO 6887-6:2013(E)

Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Diluents and disinfectants . 2
5.1 Diluents for special purposes . 2
5.2 Disinfectants for use during laboratory examination . 3
6 Apparatus and glassware . 3
7 Types of sample that can be sent to the laboratory . 3
8 Preparation of samples. 3
8.1 General . 3
8.2 Storage . 4
9 Specific procedures. 4
9.1 Procedures carried out on samples taken at farm . 4
9.2 Procedures carried out on samples taken from slaughterhouse . 6
9.3 Procedures for samples taken from poultry at hatchery or when transporting from
hatchery to farm . 7
10 Further decimal dilutions . 8
Bibliography . 9
© ISO 2013 – All rights reserved iii

---------------------- Page: 9 ----------------------

SIST EN ISO 6887-6:2013
ISO 6887-6:2013(E)

Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International
Standards adopted by the technical committees are circulated to the member bodies for voting.
Publication as an International Standard requires approval by at least 75 % of the member bodies
casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 6887-6 was prepared by the European Committee for Standardization (CEN) Technical Committee
CEN/TC 275, Food analysis — Horizontal methods, in collaboration with Technical Committee ISO/TC 34,
Food products, Subcommittee SC 9, Microbiology, in accordance with the Agreement on technical
cooperation between ISO and CEN (Vienna Agreement).
1)
ISO 6887 consists of the following parts, under the general title Microbiology of food and animal feed —
Preparation of test samples, initial suspension and decimal dilutions for microbiological examination:
— Part 1: General rules for the preparation of the initial suspension and decimal dilutions
— Part 2: Specific rules for the preparation of meat and meat products
— Part 3: Specific rules for the preparation of fish and fishery products
— Part 4: Specific rules for the preparation of products other than milk and milk products, meat and meat
products, and fish and fishery products
— Part 5: Specific rules for the preparation of milk and milk products
— Part 6: Specific rules for the preparation of samples taken at the primary production stage
1) It is intended that, upon revision, the main element of Parts 2 to 5 will be aligned with the main element of the
title of Part 6 (i.e. “Microbiology of food and animal feed”).
iv © ISO 2013 – All rights reserved

---------------------- Page: 10 ----------------------

SIST EN ISO 6887-6:2013
INTERNATIONAL STANDARD ISO 6887-6:2013(E)
Microbiology of food and animal feed — Preparation of
test samples, initial suspension and decimal dilutions for
microbiological examination —
Part 6:
Specific rules for the preparation of samples taken at the
primary production stage
1 Scope
This part of ISO 6887 specifies rules for the preparation of samples taken at all stages from the farm
to the slaughterhouse and their suspension for microbiological examination when the samples require
different preparation from the methods described in ISO 6887-1. ISO 6887-1 defines the general rules for
the preparation of the initial suspension and decimal dilutions for microbiological examination.
This part of ISO 6887 excludes the preparation of samples for both enumeration and detection test
methods where preparation details are specified in the relevant International Standards.
This part of ISO 6887 is applicable to various samples taken from the hatchery, the farm, from the
vehicle or the animals during transportation, or from animals or their carcasses in the slaughterhouse,
to indicate the microbiological status of the animals in relation to zoonotic agents. This part of ISO 6887
does not apply to samples taken to assess the hygiene of meat. These are covered by ISO 6887-2.
This part of ISO 6887 does not consider samples taken from the aquatic environment (marine or
freshwater) at the primary production stage. These are covered by ISO 6887-3.
2 Normative references
The following documents, in whole or in part, are normatively referenced in this document and are
indispensable for its application. For dated references, only the edition cited applies. For undated
references, the latest edition of the referenced document (including any amendments) applies.
ISO 6887-1, Microbiology of food and animal feeding stuffs — Preparation of test samples, initial suspension
and decimal dilutions for microbiological examination — Part 1: General rules for the preparation of the
1)
initial suspension and decimal dilutions
ISO 7218:2007, Microbiology of food and animal feeding stuffs — General requirements and guidance for
microbiological examinations
ISO 13307, Microbiology of food and animal feed — Primary production stage — Sampling techniques
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
laboratory sample
sample prepared for sending to the laboratory and intended for inspection or testing
© ISO 2013 – All rights reserved 1

---------------------- Page: 11 ----------------------

SIST EN ISO 6887-6:2013
ISO 6887-6:2013(E)

3.2
test portion
measured (volume or mass) representative sample taken from the laboratory sample (3.1) for use in the
preparation of the initial suspension (3.4)
3.3
pooled sample
composite sample taken from a number of different animals or composites of separate environmental
samples
3.4
initial suspension
primary dilution
suspension, solution or emulsion obtained after a weighed or measured quantity of the product under
examination (or of a test sample prepared from the product) has been mixed with, normally, a nine-fold
quantity of diluent, allowing large particles, if present, to settle
Note 1 to entry: Sometimes higher or lower initial dilutions are prepared, for example 1 in 5 or 1 in 100 (V → V ).
1 2
3.5
further decimal dilutions
suspension or solution obtained by mixing a measured volume of the initial suspension (3.4) with a nine-
fold volum
...

Questions, Comments and Discussion

Ask us and Technical Secretary will try to provide an answer. You can facilitate discussion about the standard in here.