kSIST FprEN 49-1:2026

• Abstract:

  This document specifies a method for the determination of the protective effectiveness or the toxic values of a wood preservative against infestation by Anobium punctatum (De Geer) when the product is applied as a surface treatment to wood.; This method is applicable to:; - water-insoluble chemicals that are being studied as active insecticides,; - organic formulations, as supplied or as prepared in the laboratory by dilution of concentrates,; - organic water-dispersible formulations as supplied or as prepared in the laboratory by dilution of concentrates, or; - water-soluble materials, for example salts.; The method is applicable whether or not the test specimens have been subjected to appropriate ageing procedures.

• Status: Not Published
• Public Enquiry End Date: 24-Dec-2025
• ICS: 71.100.50 - Wood-protecting chemicals
• Technical Committee: LLZ - Les, lesni izdelki in zaščita lesa
• Current Stage: 5020 - Formal vote (FV) (Adopted Project)
• Start Date: 06-Aug-2026
• Due Date: 24-Sep-2026
• Completion Date: 07-Aug-2026
• Ref Project: FprEN 49-1 - Wood preservatives - Determination of the protective effectiveness against Anobium punctatum (De Geer) by egg-laying and larval survival - Part 1: Application by surface treatment (Laboratory method)

• Revises: SIST EN 49-1:2016 - Wood preservatives - Determination of the protective effectiveness against Anobium punctatum (De Geer) by egg-laying and larval survival - Part 1: Application by surface treatment (Laboratory method)
• Effective Date: 12-Apr-2023

kSIST FprEN 49-1:2026 is SIST's draft European standard for testing wood preservatives against Anobium punctatum (De Geer) when the product is applied as a surface treatment to wood. It specifies a laboratory method for determining protective effectiveness, or toxic values, by observing egg laying, egg hatch and larval survival, so manufacturers, test laboratories and buyers can assess whether a preservative works on treated timber.

What does kSIST FprEN 49-1:2026 specify?

kSIST FprEN 49-1:2026 specifies a laboratory method for surface-treated wood to measure how well a wood preservative protects against infestation by Anobium punctatum and, when required, to determine toxic values. The method applies to water-insoluble chemicals, organic formulations, organic water-dispersible formulations and water-soluble materials such as salts.

The document is organized as a test method with clauses for scope, references, terms, principle, test materials, sampling, test specimens, procedure, validity, results and test report. Its annexes support the method with an example report, insect sex identification, culturing guidance and safety precautions.

Annex	What it covers
Annex A	Example of a test report
Annex B	Identification of sex of test insects Anobium punctatum
Annex C	Culturing technique for Anobium punctatum
Annex D	Environmental, health and safety precautions within chemical/biological laboratory

EN 49-1 is the part for application by surface treatment. The companion part EN 49-2 covers application by impregnation.

What are the key requirements of kSIST FprEN 49-1:2026?

Test principle and what is measured

kSIST FprEN 49-1:2026 exposes treated wood specimens to gravid female Anobium punctatum and compares the number of eggs laid, eggs hatched and live larvae with untreated controls. In practice, the test shows whether the preservative stops infestation from starting and whether larvae survive in treated wood.

When toxic values are required, the standard uses several treatment concentrations and identifies the point where all larvae die and the next lower concentration where live larvae still occur. That gives a practical loading range for comparing products and deciding whether a formulation is effective enough for use.

Test insects, materials and chamber conditions

kSIST FprEN 49-1:2026 uses adult Anobium punctatum collected from natural infestation or laboratory culture, checked to be active, undamaged and free from mites, and then separated by sex. Annex B helps identify males and females, while Annex C gives culturing guidance. In practice, this matters because poor insect quality can invalidate the test.

The method also specifies the support materials and environment: paraffin wax for water-based systems, a resistant sealant for organic-solvent systems, starch-free paste for attaching cloth, grade 3 water to EN ISO 3696, and suitable solvent or diluent with no toxic residue. It also sets controlled chamber conditions for culturing, conditioning and testing, so results are repeatable between laboratories.

Wood species, specimen quality and numbers

kSIST FprEN 49-1:2026 uses European oak as the reference species, either sessile oak or pedunculate oak. The wood must be sapwood, free from defects, and prepared into test pieces of the specified size and orientation. That keeps the wood itself from dominating the result.

Clause 7 also sets the specimen count: five test specimens for each preservative and concentration, five untreated controls, and five solvent or diluent controls when relevant. Using enough controls is important because the test validity depends on the insects performing properly on untreated wood.

Application, sealing and ageing

kSIST FprEN 49-1:2026 allows treatment by dipping, brushing or pipette, but the sealing of the specimen faces depends on the application method. For dipping, all faces except one broad face are sealed; for brushing or pipette, only the transverse faces are sealed. This keeps the treated area defined so the application rate can be calculated consistently.

The document requires freshly prepared treatment solutions and gives rules for solids, liquids and series of concentrations for toxic values. After treatment, specimens are conditioned for four weeks, with longer conditioning allowed for slow-drying products, and any ageing by EN 73 or EN 84 is carried out after drying. In practice, this separates product performance from simple solvent effects and makes the treated wood closer to real service conditions.

Exposure, examination and validity

kSIST FprEN 49-1:2026 attaches a fine cloth egg-laying zone to the unsealed face, then places each specimen with five female insects and at least five male insects in a test container. The specimens stay in the testing chamber for about one week, eggs are counted, and additional insect groups may be added if needed. Later examination can be at 26 weeks or 52 weeks after the last insects are introduced, depending on the expected mode of action.

Clause 9 makes the test valid only if each control set yields more than 50 live larvae and live larvae are present in all control specimens. That validity check matters because the result is only meaningful if the insects were able to infest untreated wood.

Reporting

kSIST FprEN 49-1:2026 requires the report to identify the standard, supplier, preservative, active ingredient, wood species, application method, concentration, treatment date, drying method, ageing, exposure and examination dates, and the detailed counts for each specimen. If toxic values are determined, they must be reported as loading per treated wood surface together with the corresponding solution concentrations. This allows another laboratory to understand exactly how the result was obtained.

What terms does kSIST FprEN 49-1:2026 define?

• Representative sample - a sample whose physical or chemical characteristics match the volumetric average of the total material being sampled.
• Supplier - the sponsor of the test, meaning the person or company that provides the wood preservative sample.
• Protective effectiveness - the measured ability of a preservative-treated specimen to resist egg laying, egg hatch and larval survival.
• Toxic values - the loading levels used to identify where larvae are killed and where live larvae still appear at the next lower concentration.
• Gravid females - female beetles ready to lay eggs, used to challenge the treated specimens.
• Control test specimens - untreated or solvent/diluent-treated specimens used as the comparison base for validity and performance.
• Test specimen - a prepared wood piece treated and exposed under the method to assess preservative performance.

Who uses kSIST FprEN 49-1:2026?

This standard is used by laboratories that test wood preservatives, by manufacturers developing insecticidal or preservative formulations, and by quality or regulatory teams that need documented performance data. It is also relevant to technical buyers and specifiers who need evidence that a surface-treatment product has been assessed in a standardized way.

The method is especially useful when the preservative is intended for dip, brush or spray applications on timber and the question is whether egg laying and larval survival are reduced under laboratory conditions.

What changed in kSIST FprEN 49-1:2026 from the previous edition?

kSIST FprEN 49-1:2026 supersedes EN 49-1:2016. The foreword lists these technical changes:

• Sealing requirements for specimens treated with solutions in which an organic solvent is the continuous phase were generalized.
• Temperature and humidity requirements were aligned with other insect test standards.
• Imaging facilities for non-destructive recording of insect tunnels inside specimens were generalized.
• Storage limitations for wood used to make test specimens were deleted.
• A note was added to allow, in some cases, fewer concentrations for determining toxic value.
• Dipping specifications were generalized and aligned with other insect standards.

Which standards are used with kSIST FprEN 49-1:2026?

• EN 73 - provides the evaporative accelerated ageing procedure used when ageing is required before biological testing.
• EN 84 - provides the leaching accelerated ageing procedure used when ageing is required before biological testing.
• EN ISO 3696 - specifies the quality of water used in the test.
• EN 212 - gives general guidance for sampling and preparation of wood preservatives and treated timber.
• EN 49-2 - covers the same biological test concept for preservative application by impregnation rather than surface treatment.

What does the kSIST FprEN 49-1:2026 document contain?

The document contains the full laboratory workflow for this insect-resistance test: specimen selection and preparation, face sealing, treatment by dipping, brush or pipette, drying and conditioning, exposure to insects, examination, validity checks and reporting. It also gives the calculation approach for retention, application rate and toxic values, so the user can turn raw test observations into comparable performance data.

Annex A provides a worked example of a test report, including the way results are presented. Annex B shows how to distinguish male and female Anobium punctatum, Annex C describes how to culture the insects, and Annex D lists laboratory safety and environmental precautions.

SLOVENSKI STANDARD
oSIST prEN 49-1:2025
01-december-2025
Biocidni proizvodi za zaščito lesa - Ugotavljanje učinkovitosti preventivne zaščite
proti navadnemu trdoglavcu Anobium punctatum (De Geer) glede na število
odloženih jajčec in preživelih ličink - 1. del: Površinsko nanašanje (laboratorijska
metoda)
Wood preservatives - Determination of the protective effectiveness against Anobium
punctatum (De Geer) by egg-laying and larval survival - Part 1: Application by surface
treatment (Laboratory method)
Holzschutzmittel - Bestimmung der vorbeugenden Wirkung gegenüber Anobium
punctatum (De Geer) durch Beobachten der Eiablage und des Überlebens von Larven -
Teil 1: Oberflächenverfahren (Laboratoriumsverfahren)
Produits de préservation du bois - Détermination de l'efficacité protectrice vis-à-vis
d'Anobium punctatum (De Geer) par l'observation de la ponte et du taux de survie des
larves - Partie 1 : Application par traitement de surface (Méthode de laboratoire)
Ta slovenski standard je istoveten z: prEN 49-1
ICS:
71.100.50 Kemikalije za zaščito lesa Wood-protecting chemicals
oSIST prEN 49-1:2025 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

oSIST prEN 49-1:2025
oSIST prEN 49-1:2025
DRAFT
EUROPEAN STANDARD
prEN 49-1
NORME EUROPÉENNE
EUROPÄISCHE NORM
October 2025
ICS 71.100.50 Will supersede EN 49-1:2016
English Version
Wood preservatives - Determination of the protective
effectiveness against Anobium punctatum (De Geer) by
egg-laying and larval survival - Part 1: Application by
surface treatment (Laboratory method)
Produits de préservation du bois - Détermination de Holzschutzmittel - Bestimmung der vorbeugenden
l'efficacité protectrice vis-à-vis d'Anobium punctatum Wirkung gegenüber Anobium punctatum (De Geer)
(De Geer) par l'observation de la ponte et du taux de durch Beobachten der Eiablage und des Überlebens
survie des larves - Partie 1 : Application par traitement von Larven - Teil 1: Oberflächenverfahren
de surface (Méthode de laboratoire) (Laboratoriumsverfahren)
This draft European Standard is submitted to CEN members for enquiry. It has been drawn up by the Technical Committee
CEN/TC 38.
If this draft becomes a European Standard, CEN members are bound to comply with the CEN/CENELEC Internal Regulations
which stipulate the conditions for giving this European Standard the status of a national standard without any alteration.

This draft European Standard was established by CEN in three official versions (English, French, German). A version in any other
language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC
Management Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of which they are
aware and to provide supporting documentation.

Warning : This document is not a European Standard. It is distributed for review and comments. It is subject to change without
notice and shall not be referred to as a European Standard.

EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATIO N

EUROPÄISCHES KOMITEE FÜR NORMUN G

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2025 CEN All rights of exploitation in any form and by any means reserved Ref. No. prEN 49-1:2025 E
worldwide for CEN national Members.

oSIST prEN 49-1:2025
prEN 49-1:2025 (E)
Contents Page
European foreword . 4
Introduction . 5
1 Scope . 6
2 Normative references . 6
3 Terms and definitions . 6
4 Principle . 7
5 Test materials . 7
5.1 Biological material . 7
5.2 Products and reagents . 7
5.3 Apparatus . 8
6 Sampling . 8
7 Test specimens . 9
7.1 Species of wood . 9
7.2 Wood quality . 9
7.3 Provision of test specimens . 9
7.4 Dimensions of test specimens . 9
7.5 Number of test specimens . 9
8 Procedure. 10
8.1 Preparation of the test specimens . 10
8.1.1 Conditioning of test specimens prior to sealing . 10
8.1.2 Sealing of the test specimen faces . 10
8.1.3 Treatment of the test specimens . 10
8.1.4 Drying and conditioning of the test specimens after treatment . 12
8.2 Exposure of the test specimens to the insects . 12
8.3 Conditions and duration of the test . 12
8.4 Examination of the test specimens . 13
9 Validity of test . 13
10 Expression of results . 13
10.1 Assessment of the protective effectiveness . 13
10.2 Toxic values . 13
11 Test report . 14
Annex A (informative) Example of a test report . 16
Annex B (informative) Identification of sex of test insects Anobium punctatum . 18
Annex C (informative) Culturing technique for Anobium punctatum. 19
C.1 Culture wood . 19
C.1.1 Wood species . 19
C.1.2 Collection of culture wood . 19
C.1.3 Cutting of culture wood . 19
oSIST prEN 49-1:2025
prEN 49-1:2025 (E)
C.1.4 Drying of culture wood . 19
C.2 Source of beetles . 19
C.2.1 Collection of beetles . 19
C.2.2 Quarantine of beetles . 19
C.3 Infestation of culture wood . 19
C.3.1 Culture vessels . 19
C.3.2 Preparation of wood . 19
C.3.3 Introduction of beetles . 20
C.4 Culturing conditions . 20
C.4.1 Normal environment . 20
C.4.2 Natural pupation induction . 20
C.4.3 Artificial pupation induction . 20
C.5 Collection of beetles . 20
C.6 General culture hygiene . 20
Annex D (informative) Environmental, health and safety precautions within chemical/biological
laboratory . 22
Bibliography . 23

oSIST prEN 49-1:2025
prEN 49-1:2025 (E)
European foreword
This document (prEN 49-1:2025) has been prepared by Technical Committee CEN/TC 38 “Biological
durability of wood and wood-based products”, the secretariat of which is held by SIS.
This document is currently submitted to the CEN Enquiry.
This document will supersede EN 49-1:2016.
Significant technical differences between this document and EN 49-1:2016 are as follows:
a) Specifications for sealing faces of test specimens to be treated with solutions in which an organic
solvent is the continuous phase were generalized (5.2.2)
b) Requirements for temperature and humidity were aligned with other insect test standards (5.3.1;
5.3.5)
c) imaging facilities for non-destructive recordings of insect tunnels inside test specimens were
generalized (5.3.10)
d) storage limitations for wood from which test specimens are to be made from were deleted (7.2)
e) a note was added to 8.1.3.1.3 to optionally reduce the number of concentrations which are necessary
to determine the toxic value
f) Specifications for dipping (8.1.3.2.1) were generalized and aligned with other insect standards.
NOTE Test results obtained according to earlier versions of this document or when the tests had started before
this version of EN 49-1 was published are considered as valid.
EN 49, Wood preservatives ― Determination of the protective effectiveness against Anobium punctatum (De
Geer) by egg-laying and larval survival, consists of two parts:
— Part 1: Application by surface treatment (Laboratory method);
— Part 2: Application by impregnation (Laboratory method).
EN 49-1 is required to enable effectiveness assessments of wood preservatives which are intended to be
applied by surface treatment and EN 49-2 those which are intended to be applied by impregnation.

oSIST prEN 49-1:2025
prEN 49-1:2025 (E)
Introduction
This document describes a laboratory method of testing which gives a basis for assessment of the
effectiveness of a wood preservative, when applied as a surface treatment, against Anobium punctatum. It
allows the determination of the concentration at which the product prevents the development of infestation
from egg laying. It can also be used with formulations ready for use.
This document provides information for the sealing of all but one lateral face when specimens are to be
treated by dipping.
The method simulates conditions that can occur in practice on timber which has been treated some time
previously with wood preservative applied by dip, brush or spray and on which eggs of Anobium punctatum
are laid.
This laboratory method provides one criterion by which the value of a product can be assessed. In making
this assessment the methods by which the preservative may be applied should be taken into account. It is
further recommended that results from this test should be supplemented by those from other appropriate
tests, and above all by comparison with practical experience.
When products which are very active at low concentrations are used it is very important to take suitable
precautions to isolate and separate, as far as possible, operations involving chemical products, other
products, treated wood, laboratory apparatus and clothing. Suitable precautions should include the use of
separate rooms, areas within rooms, extraction facilities, conditioning chambers and special training for
personnel (see also Annex D for environmental, health and safety precautions).

oSIST prEN 49-1:2025
prEN 49-1:2025 (E)
1 Scope
This document specifies a method for the determination of the protective effectiveness or the toxic values of
a wood preservative against infestation by Anobium punctatum (De Geer) when the product is applied as a
surface treatment to wood.
This method is applicable to:
— water-insoluble chemicals that are being studied as active insecticides;
— organic formulations, as supplied or as prepared in the laboratory by dilution of concentrates;
— organic water-dispersible formulations as supplied or as prepared in the laboratory by dilution of
concentrates;
— water-soluble materials, for example salts.
The method is applicable whether or not the test specimens have been subjected to appropriate ageing
procedures.
2 Normative references
The following documents, in whole or in part, are normatively referenced in this document and are
indispensable for its application. For dated references, only the edition cited applies. For undated references,
the latest edition of the referenced document (including any amendments) applies.
EN 73, Durability of wood and wood-based products — Accelerated ageing of treated wood prior to biological
testing — Evaporative ageing procedure
EN 84, Durability of wood and wood-based products — Accelerated ageing of treated wood prior to biological
testing — Leaching procedure
EN ISO 3696, Water for analytical laboratory use — Specification and test methods (ISO 3696)
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
representative sample
sample having its physical or chemical characteristics identical to the volumetric average characteristics of
the total volume being sampled
3.2
supplier
sponsor of the test (person or company providing the sample of wood preservative to be tested)
oSIST prEN 49-1:2025
prEN 49-1:2025 (E)
4 Principle
Depending on the test being carried out either:
— on a set of test specimens of a susceptible wood species that is surface treated with a solution of the
preservative, or
— if toxic values are to be determined, on several sets of test specimens of a susceptible wood species that
are surface treated with a series of solutions in which the concentration of preservative is ranged in a
given progression.
The treated test specimens are exposed to gravid females of Anobium punctatum. The number of eggs laid,
the number of eggs hatched and the numbers of surviving larvae are observed and compared with those in
untreated control test specimens. If the preservative has been prepared in the laboratory by dilution of a
concentrate or by dissolution of a solid, the resulting attack is also compared to that in solvent or diluent
treated control test specimens.
5 Test materials
5.1 Biological material
Anobium punctatum (De Geer)
Adult males and females in good condition.
Adults to be used in the test shall be collected at daily intervals from naturally infested wood or laboratory
culture (see Annex C).
Use recently emerged adults which have been recently collected; kept overnight in quarantine (see C.6); and
then checked to ensure that they are undamaged, active and free from any infestation by mites. Determine
the sex (see Annex B) of the collected and checked adults and place the males and females in separate
containers.
NOTE The proportion of males and females varies during the emergence period.
5.2 Products and reagents
5.2.1 Paraffin wax, for sealing the relevant faces of test specimens to be treated with solutions in which
water is the continuous phase (see 8.1.2.1 and 8.1.2.2).
NOTE Paraffin wax with a setting point of approx. 52 °C to 54 °C has been found to be suitable.
5.2.2 Sealant, for sealing the end faces and all relevant other faces of test specimens to be treated with
solutions in all cases in which an organic solvent is the continuous phase (see 8.1.2.1 and 8.1.2.3). The
sealant must be resistant to penetration by the wood preservative under test and the respective solvent. The
sealant must not act repellent or insecticidal in the test specimen.
NOTE Gelatine or two to three coats of a two-component epoxy varnish with a drying step between each coat has
been found to be suitable.
5.2.3 Paste, for securing fine cloth (5.2.7). The paste shall be starch-free, non-toxic to Anobium punctatum
and insoluble in the product under test.
NOTE Sodium carboxy methyl cellulose, food grade, has been found to be suitable.
5.2.4 Water, complying with grade 3 of EN ISO 3696 shall be used.
oSIST prEN 49-1:2025
prEN 49-1:2025 (E)
5.2.5 Solvent or diluent, a volatile liquid that will dissolve or dilute the preservative but does not leave a
residue in the wood at the end of the post-treatment conditioning period that has a toxic effect on the
insects.
CAUTION — Do not use benzene or other solvents which pose a health risk.
5.2.6 Filter paper, ordinary quality, medium-fast grade.
5.2.7 Fine cloth of a suitable material with a mesh aperture of 0,3 mm to 0,6 mm for the preparation of the
egg-laying zones.
NOTE Cotton, linen or polyamide-gauze have also been proven as suitable.
5.3 Apparatus
5.3.1 Culturing chamber, with air circulation, controlled at (21 ± 2) °C, and at relative humidity
(75 ± 5) %.
5.3.2 Conditioning chamber, well ventilated, controlled at (20 ± 2) °C and at relative humidity (65 ± 5) %.
The conditioning of test specimens may be carried out in the laboratory work area (see 5.3.4) provided that
this has the conditions specified for the conditioning chamber (see 5.3.2).
5.3.3 Treatment vessel, of a material that does not react with the preservative under test, for example of
glass for organic products and of polyethylene for salts containing fluorine.
5.3.4 Laboratory work area, well ventilated, where treatment of the test specimens is carried out.
CAUTION — It is essential to follow safety procedures for handling flammable and toxic materials.
Avoid excessive exposure of operators to solvents or their vapours.
5.3.5 Testing chamber, ventilated and air-conditioned, controlled at (22 ± 2) °C and at a relative humidity
of (75 ± 5) %.
5.3.6 Pipette, specified in EN ISO 835-1 shall be used, or brush.
5.3.7 Safety equipment, protective clothing, protective gloves appropriate for the test product and the
test solvent, to ensure the safety of the operator.
5.3.8 Test containers, suitable for holding the test specimens and of material resistant to the solvents
used, and fitted with perforated covers to provide a good exchange of air.
NOTE Jars of approximately 60 mm diameter and 100 mm height have been found to be suitable.
5.3.9 Ordinary laboratory equipment, including a balance capable of weighing to an accuracy of 0,01 g.
5.3.10 Imaging facilities e. g. X-ray apparatus, or any other device that offers good images (2D), of internal
tunnels caused by small larvae.
6 Sampling
The sample of preservative shall be representative of the product to be tested. Samples shall be stored and
handled in accordance with any written recommendations from the supplier.
For the sampling of preservatives from bulk supplies, the procedure given in EN 212 should be used.
oSIST prEN 49-1:2025
prEN 49-1:2025 (E)
7 Test specimens
7.1 Species of wood
The reference species is European oak. This shall be either sessile oak (Quercus petraea (Mattuschka)
Lieblin) or pedunculate oak (Quercus robur Linnaeus).
)
Additional tests may be carried out using other species but, if so, this should be stated in the test report.
7.2 Wood quality
The wood shall be free from visible cracks, stain, decay, insect damage and other defects. The wood shall not
have been water-stored, floated, chemically treated or steamed. The wood shall originate from trees
preferably felled in winter. The trees shall be cut immediately after felling and the timber rapidly air-dried or
kiln dried at temperatures below 60 °C.
)
The wood shall be exclusively sapwood and having between two and ten annual rings per 10 mm.
It is recommended to use test specimens of similar growth rate within a single test.
7.3 Provision of test specimens
3)
Prepare planed strips having a cross-section of (25 ± 0,5) mm × (15 ± 0,5) mm removing a minimum of
2 mm from any surfaces exposed during drying. The longitudinal faces shall be parallel to the direction of the
grain. The annual rings shall be parallel to the broad faces (contact angle of less than 5°). Make transverse
cuts, neatly to give sharp edges and a fine-sawn finish to the end-grain surfaces, to give test specimens
(50 ± 0,5) mm long.
The specimens shall originate from a minimum of three trees or shall be taken at random from a stock
originally of more than 500 test specimens.
7.4 Dimensions of test specimens
The dimensions of each test specimen after reaching equilibrium in the conditioning chamber (5.3.2) shall be
(50 ± 0,5) mm × (25 ± 0,5) mm × (15 ± 0,5) mm.
Mark each test specimen so that it can be identified throughout the test.
7.5 Number of test specimens
Use:
a) five test specimens (no more than two originating from the same tree unless taken at random from a
stock of more than 500) for each preservative and each concentration;
b) five untreated control test specimens (no more than two originating from the same tree unless taken at
random from a stock of more than 500) for a complete test of any given preservative;
c) five control test specimens (no more than two originating from the same tree unless taken at random
from a stock of more than 500) treated with that solvent or
...