oSIST prEN 49-1:2025

Wood preservatives - Determination of the protective effectiveness against Anobium punctatum (De Geer) by egg-laying and larval survival - Part 1: Application by surface treatment (Laboratory method)

This document specifies a method for the determination of the protective effectiveness or the toxic values of a wood preservative against infestation by Anobium punctatum (De Geer) when the product is applied as a surface treatment to wood.
This method is applicable to:
-   water-insoluble chemicals that are being studied as active insecticides;
-   organic formulations, as supplied or as prepared in the laboratory by dilution of concentrates;
-   organic water-dispersible formulations as supplied or as prepared in the laboratory by dilution of concentrates;
-   water-soluble materials, for example salts.
The method is applicable whether or not the test specimens have been subjected to appropriate ageing procedures.

Holzschutzmittel - Bestimmung der vorbeugenden Wirkung gegenüber Anobium punctatum (De Geer) durch Beobachten der Eiablage und des Überlebens von Larven - Teil 1: Oberflächenverfahren (Laboratoriumsverfahren)

Dieses Dokument legt ein Verfahren zur Bestimmung der vorbeugenden Wirkung oder der Grenze der Wirksamkeit eines Holzschutzmittels gegen den Befall durch Anobium punctatum (De Geer) fest, wenn das Produkt zur Oberflächenbehandlung des Holzes verwendet wird.
Das Verfahren ist anwendbar für:
   wasserunlösliche Chemikalien, die als wirksame Insektizide untersucht werden;
   organische Formulierungen im Anlieferungszustand oder in Form von im Laboratorium hergestellten Verdünnungen von Konzentraten;
   organische, in Wasser dispergierbare Formulierungen im Anlieferungszustand oder in Form von im Laboratorium hergestellten Verdünnungen von Konzentraten;
   wasserlösliche Stoffe, z. B. Salze.
Das Verfahren ist anwendbar unabhängig davon, ob die Prüfkörper einer angemessenen Alterungsbeanspruchung ausgesetzt wurden oder nicht.

Produits de préservation du bois - Détermination de l'efficacité protectrice vis-à-vis d'Anobium punctatum (De Geer) par l'observation de la ponte et du taux de survie des larves - Partie 1 : Application par traitement de surface (Méthode de laboratoire)

Le présent document spécifie une méthode de détermination de l'efficacité protectrice ou du seuil d'efficacité d'un produit de préservation du bois contre l'infestation par Anobium punctatum (De Geer) lorsque le produit est appliqué au bois par un traitement de surface.
Cette méthode s'applique :
—   aux produits chimiques non hydrosolubles étudiés en tant que matières actives insecticides ;
—   aux formulations organiques telles qu'elles sont fournies ou préparées au laboratoire par dilution de concentrés ;
—   aux formulations organiques hydrodispersables telles qu'elles sont fournies ou préparées en laboratoire par dilution de concentrés ; et
—   aux produits hydrosolubles, par exemple des sels.
La méthode est applicable que les éprouvettes aient été ou non soumises à des procédures de vieillissement appropriées.

Biocidni proizvodi za zaščito lesa - Ugotavljanje učinkovitosti preventivne zaščite proti navadnemu trdoglavcu Anobium punctatum (De Geer) glede na število odloženih jajčec in preživelih ličink - 1. del: Površinsko nanašanje (laboratorijska metoda)

General Information

• Status: Not Published
• Public Enquiry End Date: 24-Dec-2025
• ICS: 71.100.50 - Wood-protecting chemicals
• Technical Committee: LLZ - Les, lesni izdelki in zaščita lesa
• Current Stage: 4020 - Public enquire (PE) (Adopted Project)
• Start Date: 28-Oct-2025
• Due Date: 17-Mar-2026
• Ref Project: prEN 49-1 - Wood preservatives - Determination of the protective effectiveness against Anobium punctatum (De Geer) by egg-laying and larval survival - Part 1: Application by surface treatment (Laboratory method)

Relations

• Revises: SIST EN 49-1:2016 - Wood preservatives - Determination of the protective effectiveness against Anobium punctatum (De Geer) by egg-laying and larval survival - Part 1: Application by surface treatment (Laboratory method)
• Effective Date: 12-Apr-2023

Standards Content (Sample)

SLOVENSKI STANDARD
01-december-2025
Biocidni proizvodi za zaščito lesa - Ugotavljanje učinkovitosti preventivne zaščite
proti navadnemu trdoglavcu Anobium punctatum (De Geer) glede na število
odloženih jajčec in preživelih ličink - 1. del: Površinsko nanašanje (laboratorijska
metoda)
Wood preservatives - Determination of the protective effectiveness against Anobium
punctatum (De Geer) by egg-laying and larval survival - Part 1: Application by surface
treatment (Laboratory method)
Holzschutzmittel - Bestimmung der vorbeugenden Wirkung gegenüber Anobium
punctatum (De Geer) durch Beobachten der Eiablage und des Überlebens von Larven -
Teil 1: Oberflächenverfahren (Laboratoriumsverfahren)
Produits de préservation du bois - Détermination de l'efficacité protectrice vis-à-vis
d'Anobium punctatum (De Geer) par l'observation de la ponte et du taux de survie des
larves - Partie 1 : Application par traitement de surface (Méthode de laboratoire)
Ta slovenski standard je istoveten z: prEN 49-1
ICS:
71.100.50 Kemikalije za zaščito lesa Wood-protecting chemicals
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

DRAFT
EUROPEAN STANDARD
NORME EUROPÉENNE
EUROPÄISCHE NORM
October 2025
ICS 71.100.50 Will supersede EN 49-1:2016
English Version
Wood preservatives - Determination of the protective
effectiveness against Anobium punctatum (De Geer) by
egg-laying and larval survival - Part 1: Application by
surface treatment (Laboratory method)
Produits de préservation du bois - Détermination de Holzschutzmittel - Bestimmung der vorbeugenden
l'efficacité protectrice vis-à-vis d'Anobium punctatum Wirkung gegenüber Anobium punctatum (De Geer)
(De Geer) par l'observation de la ponte et du taux de durch Beobachten der Eiablage und des Überlebens
survie des larves - Partie 1 : Application par traitement von Larven - Teil 1: Oberflächenverfahren
de surface (Méthode de laboratoire) (Laboratoriumsverfahren)
This draft European Standard is submitted to CEN members for enquiry. It has been drawn up by the Technical Committee
CEN/TC 38.
If this draft becomes a European Standard, CEN members are bound to comply with the CEN/CENELEC Internal Regulations
which stipulate the conditions for giving this European Standard the status of a national standard without any alteration.

This draft European Standard was established by CEN in three official versions (English, French, German). A version in any other
language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC
Management Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of which they are
aware and to provide supporting documentation.

Warning : This document is not a European Standard. It is distributed for review and comments. It is subject to change without
notice and shall not be referred to as a European Standard.

EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATIO N

EUROPÄISCHES KOMITEE FÜR NORMUN G

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2025 CEN All rights of exploitation in any form and by any means reserved Ref. No. prEN 49-1:2025 E
worldwide for CEN national Members.

Contents Page
European foreword . 4
Introduction . 5
1 Scope . 6
2 Normative references . 6
3 Terms and definitions . 6
4 Principle . 7
5 Test materials . 7
5.1 Biological material . 7
5.2 Products and reagents . 7
5.3 Apparatus . 8
6 Sampling . 8
7 Test specimens . 9
7.1 Species of wood . 9
7.2 Wood quality . 9
7.3 Provision of test specimens . 9
7.4 Dimensions of test specimens . 9
7.5 Number of test specimens . 9
8 Procedure. 10
8.1 Preparation of the test specimens . 10
8.1.1 Conditioning of test specimens prior to sealing . 10
8.1.2 Sealing of the test specimen faces . 10
8.1.3 Treatment of the test specimens . 10
8.1.4 Drying and conditioning of the test specimens after treatment . 12
8.2 Exposure of the test specimens to the insects . 12
8.3 Conditions and duration of the test . 12
8.4 Examination of the test specimens . 13
9 Validity of test . 13
10 Expression of results . 13
10.1 Assessment of the protective effectiveness . 13
10.2 Toxic values . 13
11 Test report . 14
Annex A (informative) Example of a test report . 16
Annex B (informative) Identification of sex of test insects Anobium punctatum . 18
Annex C (informative) Culturing technique for Anobium punctatum. 19
C.1 Culture wood . 19
C.1.1 Wood species . 19
C.1.2 Collection of culture wood . 19
C.1.3 Cutting of culture wood . 19
C.1.4 Drying of culture wood . 19
C.2 Source of beetles . 19
C.2.1 Collection of beetles . 19
C.2.2 Quarantine of beetles . 19
C.3 Infestation of culture wood . 19
C.3.1 Culture vessels . 19
C.3.2 Preparation of wood . 19
C.3.3 Introduction of beetles . 20
C.4 Culturing conditions . 20
C.4.1 Normal environment . 20
C.4.2 Natural pupation induction . 20
C.4.3 Artificial pupation induction . 20
C.5 Collection of beetles . 20
C.6 General culture hygiene . 20
Annex D (informative) Environmental, health and safety precautions within chemical/biological
laboratory . 22
Bibliography . 23

European foreword
This document (prEN 49-1:2025) has been prepared by Technical Committee CEN/TC 38 “Biological
durability of wood and wood-based products”, the secretariat of which is held by SIS.
This document is currently submitted to the CEN Enquiry.
This document will supersede EN 49-1:2016.
Significant technical differences between this document and EN 49-1:2016 are as follows:
a) Specifications for sealing faces of test specimens to be treated with solutions in which an organic
solvent is the continuous phase were generalized (5.2.2)
b) Requirements for temperature and humidity were aligned with other insect test standards (5.3.1;
5.3.5)
c) imaging facilities for non-destructive recordings of insect tunnels inside test specimens were
generalized (5.3.10)
d) storage limitations for wood from which test specimens are to be made from were deleted (7.2)
e) a note was added to 8.1.3.1.3 to optionally reduce the number of concentrations which are necessary
to determine the toxic value
f) Specifications for dipping (8.1.3.2.1) were generalized and aligned with other insect standards.
NOTE Test results obtained according to earlier versions of this document or when the tests had started before
this version of EN 49-1 was published are considered as valid.
EN 49, Wood preservatives ― Determination of the protective effectiveness against Anobium punctatum (De
Geer) by egg-laying and larval survival, consists of
...