SIST EN ISO 9936:2016

Animal and vegetable fats and oils - Determination of tocopherol and tocotrienol contents by high-performance liquid chromatography (ISO 9936:2016)

This International Standard specifies a method for the determination of the contents of free α-, β-, γ-,
and δ-tocopherols and tocotrienols (referred to jointly as tocols) in animal and vegetable fats and oils
(referred to hereinafter as fats) by high-performance liquid chromatography (HPLC).
For products containing tocopherol or tocotrienol esters, it is necessary to carry out a preliminary
saponification.
Milk and milk products (or fat coming from milk and milk products) are excluded from the Scope of this
International Standard.
NOTE A suitable method involving a cold saponification procedure is described in Annex B for
information only.

Tierische und pflanzliche Fette und Öle - Bestimmung des Tocopherol- und Tocotrienol-Gehaltes mittels Hochleistungsflüssigchromatographie (ISO 9936:2016)

Diese Internationale Norm legt ein Verfahren zur Bestimmung des Gehalts an freien α-, β-, γ- und δ Tocopherolen und Tocotrienolen (nachfolgend insgesamt als Tocole bezeichnet) in tierischen und pflanzlichen Fetten und Ölen (nachfolgend als Fette bezeichnet) durch Hochleistungsflüssigchromatographie (HPLC, en: high-performance liquid chromatography) fest.
Bei Produkten, die Ester von Tocopherolen oder Tocotrienolen enthalten, ist es erforderlich, eine voraus-gehende Verseifung durchzuführen.
Milch und Milcherzeugnisse (oder Fett aus Milch und Milcherzeugnissen) sind vom Anwendungsbereich dieser Internationalen Norm ausgenommen.
ANMERKUNG   Ein geeignetes Verfahren mit einer Kaltverseifung wird in Anhang B nur zur Information beschrieben.

Corps gras d'origines animale et végétale -- Détermination des teneurs en tocophérols et en tocotriénols par chromatographie en phase liquide à haute performance (ISO 9936:2016)

ISO 9936:2016 spécifie une méthode pour la détermination des teneurs en α-, β-, γ- et δ-tocophérols et tocotriénols libres (appelés globalement tocols) des graisses et des huiles (appelés corps gras dans la suite du texte) d'origines animale et végétale, par chromatographie en phase liquide haute performance (CLHP).
Pour les produits contenant des esters de tocophérols ou de tocotriénols, il est nécessaire qu'ils subissent une saponification préalable.
Le lait et les produits laitiers (ou les corps gras issus du lait et des produits laitiers) sont exclus du domaine d'application de la présente Norme internationale.
NOTE          Une méthode appropriée incluant un protocole de saponification à froid est décrite, pour information uniquement, dans l'Annexe B.

Rastlinske in živalske maščobe in olja - Določevanje tokoferola in tokotrienola s tekočinsko kromatografijo visoke ločljivosti (ISO 9936:2016)

Ta mednarodni standard določa metodo za določevanje vsebnosti prostih α-, β-, γ- in δ-tokoferolov ter tokotrienolov (s skupnim imenom tokoli) v živalskih in rastlinskih maščobah ter oljih (v nadaljevanju »maščobe«) s tekočinsko kromatografijo visoke ločljivosti (HPLC).
Za proizvode, ki vsebujejo tokoferolne ali tokotrienolne estre, je treba izvesti predhodno
umiljenje. Mleko in mlečni izdelki (ali maščoba iz mleka in mlečnih izdelkov) niso zajeti v tem mednarodnem standardu.
OPOMBA: Ustrezna metoda, ki vključuje postopek hladnega umiljenja, je v dodatku B opisana
samo informativno.

General Information

• Status: Published
• Public Enquiry End Date: 28-Feb-2016
• Publication Date: 17-May-2016
• ICS: 67.200.10 - Animal and vegetable fats and oils
• ICS: 71.040.50 - Physicochemical methods of analysis
• Technical Committee: KŽP - Agricultural food products
• Current Stage: 6060 - National Implementation/Publication (Adopted Project)
• Start Date: 22-Apr-2016
• Due Date: 27-Jun-2016
• Completion Date: 18-May-2016
• Ref Project: EN ISO 9936:2016 - Animal and vegetable fats and oils - Determination of tocopherol and tocotrienol contents by high-performance liquid chromatography (ISO 9936:2016)

Relations

• Replaces: SIST EN ISO 9936:2006 - Animal and vegetable fats and oils - Determination of tocopherol and tocotrienol contents by high-performance liquid chromatography (ISO 9936:2006)
• Effective Date: 08-Jun-2022
• Replaces: SIST EN ISO 9936:2006 - Animal and vegetable fats and oils - Determination of tocopherol and tocotrienol contents by high-performance liquid chromatography (ISO 9936:2006)
• Effective Date: 01-Jun-2016
• Replaces: SIST EN ISO 9936:2006/AC:2009 - Animal and vegetable fats and oils - Determination of tocopherol and tocotrienol contents by high-performance liquid chromatography (ISO 9936:2006/Cor 1:2008)
• Effective Date: 01-Jun-2016
• Replaces: SIST EN ISO 9936:2006/A1:2011 - Animal and vegetable fats and oils - Determination of tocopherol and tocotrienol contents by high-performance liquid chromatography - Amendment 1: Updating of reagents and confirmation of statistical data validity (ISO 9936:2006/Amd 1:2011)
• Effective Date: 01-Jun-2016

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN ISO 9936:2016
01-junij-2016
1DGRPHãþD
SIST EN ISO 9936:2006
SIST EN ISO 9936:2006/A1:2011
SIST EN ISO 9936:2006/AC:2009
5DVWOLQVNHLQåLYDOVNHPDãþREHLQROMD'RORþHYDQMHWRNRIHURODLQWRNRWULHQRODV
WHNRþLQVNRNURPDWRJUDILMRYLVRNHORþOMLYRVWL,62
Animal and vegetable fats and oils - Determination of tocopherol and tocotrienol contents
by high-performance liquid chromatography (ISO 9936:2016)
Tierische und pflanzliche Fette und Öle - Bestimmung des Tocopherol- und Tocotrienol-
Gehaltes mittels Hochleistungsflüssigchromatographie (ISO 9936:2016)
Corps gras d'origines animale et végétale -- Détermination des teneurs en tocophérols et
en tocotriénols par chromatographie en phase liquide à haute performance (ISO
9936:2016)
Ta slovenski standard je istoveten z: EN ISO 9936:2016
ICS:
67.200.10 5DVWOLQVNHLQåLYDOVNH Animal and vegetable fats
PDãþREHLQROMD and oils
71.040.50 Fizikalnokemijske analitske Physicochemical methods of
metode analysis
SIST EN ISO 9936:2016 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN ISO 9936:2016

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SIST EN ISO 9936:2016


EN ISO 9936
EUROPEAN STANDARD

NORME EUROPÉENNE

April 2016
EUROPÄISCHE NORM
ICS 67.200.10 Supersedes EN ISO 9936:2006
English Version

Animal and vegetable fats and oils - Determination of
tocopherol and tocotrienol contents by high-performance
liquid chromatography (ISO 9936:2016)
Corps gras d'origines animale et végétale - Tierische und pflanzliche Fette und Öle - Bestimmung
Détermination des teneurs en tocophérols et en des Tocopherol- und Tocotrienol-Gehaltes mittels
tocotriénols par chromatographie en phase liquide à Hochleistungsflüssigchromatographie (ISO 9936:2016)
haute performance (ISO 9936:2016)
This European Standard was approved by CEN on 4 March 2016.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2016 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 9936:2016 E
worldwide for CEN national Members.

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SIST EN ISO 9936:2016
EN ISO 9936:2016 (E)
Contents Page
European foreword . 3

2

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SIST EN ISO 9936:2016
EN ISO 9936:2016 (E)
European foreword
This document (EN ISO 9936:2016) has been prepared by Technical Committee ISO/TC 34 “Food
products” in collaboration with Technical Committee CEN/TC 307 “Oilseeds, vegetable and animal fats
and oils and their by-products - Methods of sampling and analysis” the secretariat of which is held by
AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by October 2016, and conflicting national standards shall
be withdrawn at the latest by October 2016.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent
rights.
This document supersedes EN ISO 9936:2006.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy,
...