oSIST prEN ISO 17174:2023

Molecular biomarker analysis - DNA barcoding of fish and fish products using defined mitochondrial cytochrome b and cytochrome c oxidase I gene segments (ISO/DIS 17174:2023)

This document describes a procedure for the identification of single fish and fish fillets to the
level of genus or species.
The identification of fish species is carried out by PCR amplification of either a segment of the
mitochondrial cytochrome b gene (cytb) [1] or the cytochrome c oxidase I gene (cox1, syn COI)
[2], [3] or both, followed by sequencing of the PCR products and subsequent sequence
comparison with entries in databases [4], [5]. The methodology allows the identification of a large
number of commercially important fish species.
The decision whether the cytb or cox1 gene segment or both are used for fish identification
depends on the declared fish species, the applicability of the PCR method for the fish species
and the availability of comparative sequences in the public databases.
This method has been successfully validated on raw fish fillets, however, laboratory experience
is available that it can also be applied to processed, e.g. cold smoked, hot smoked, salted,
frozen, cooked, fried, deep fried
samples.
This document is usually unsuitable for the analysis of highly processed foods, e.g. tins of fish,
with highly degraded DNA where the fragment lengths are not sufficient for amplification of the
targets. Furthermore, it is not applicable for complex fish products containing mixtures of two or
more fish species.

Untersuchung auf molekulare Biomarker - DNA-Barcoding von Fisch und Fischprodukten anhand definierter mitochondrialer Cytochrom b- und Cytochrom c-Oxidase I-Genabschnitte (ISO/DIS 17174:2023)

Dieses Dokument legt ein Verfahren für die Identifizierung von einzelnen Fischen und Fischfilets bis zur Ebene der Gattung oder Art fest. Es ermöglicht die Identifizierung einer großen Anzahl kommerziell wichtiger Fischarten mittels DNA-Barcoding.
Dieses Verfahren wurde an rohem Fisch validiert. Laborerfahrungen deuten auf eine zusätzliche Anwendbarkeit auf verarbeitete Fischerzeugnisse hin, z. B. kalt geräucherte, heiß geräucherte, gesalzene, tiefgefrorene, gekochte, gebratene und frittierte Proben.
Das beschriebene Verfahren ist in der Regel ungeeignet für die Analyse stark verarbeiteter Lebensmittel, z. B. Fischkonserven, mit stark degradierter DNA, bei denen die Fragmentlängen nicht für eine Amplifikation der Zielsequenzen (Targets) ausreichen. Außerdem ist es nicht anwendbar auf zusammengesetzte Fischerzeugnisse, die Mischungen aus zwei oder mehr Fischarten enthalten.
Die Identifizierung der Fischarten erfolgt durch PCR-Amplifikation entweder eines Segments des mitochondrialen Cytochrom-b-Gens (cytb) oder des Cytochrom-c-Oxidase-I-Gens (cox1, syn COI) oder von beiden, gefolgt von der Sequenzierung der PCR-Produkte und einem anschließenden Sequenzvergleich mit Einträgen in Datenbanken.

Analyse de biomarqueurs moléculaires - Codes-barres d’ADN de poissons et de produits à base de poisson à l’aide de segments de gènes mitochondriaux de cytochrome b et cytochrome c oxydase I (ISO/DIS 17174:2023)

Analiza molekularnih biomarkerjev - Črtno kodiranje DNK rib in ribjih proizvodov z uporabo definiranih mitohondrijskih segmentov genov citokrom b in citokrom c oksidaze I (ISO/DIS 17174:2023)

General Information

• Status: Not Published
• Public Enquiry End Date: 01-Aug-2023
• ICS: 67.120.30 - Fish and fishery products
• Technical Committee: KŽP - Agricultural food products
• Current Stage: 4020 - Public enquire (PE) (Adopted Project)
• Start Date: 05-Jun-2023
• Due Date: 23-Oct-2023
• Completion Date: 03-Aug-2023
• Directive: 2017/625 - Regulation (EU) 2017/625 of the European Parliament and of the Council of 15 March 2017 on official controls and other official activities performed to ensure the application of food and feed law, rules on animal health and welfare, plant health and plant protection products
• Directive: 882/2004 - Regulation (EC) No 882/2004 of the European Parliament and of the Council of 29 April 2004 onofficial controls performed to ensure the verification of compliance with feed and food law, animal health and animal welfare rules
• Ref Project: prEN ISO 17174 - Molecular biomarker analysis - DNA barcoding of fish and fish products using defined mitochondrial cytochrome b and cytochrome c oxidase I gene segments (ISO/DIS 17174:2023)

Relations

• Revises: SIST-TS CEN/TS 17303:2019 - Foodstuffs - DNA barcoding of fish and fish products using defined mitochondrial cytochrome b and cytochrome c oxidase I gene segments
• Effective Date: 18-Jan-2023

Standards Content (Sample)

SLOVENSKI STANDARD
oSIST prEN ISO 17174:2023
01-julij-2023
Analiza molekularnih biomarkerjev - Črtno kodiranje DNK rib in ribjih proizvodov z
uporabo segmentov genov, ki nosijo zapis za mitohondrijski citokrom b in
citokrom c oksidaze I (ISO/DIS 17174:2023)
Molecular biomarker analysis - DNA barcoding of fish and fish products using defined
mitochondrial cytochrome b and cytochrome c oxidase I gene segments (ISO/DIS
17174:2023)
Untersuchung auf molekulare Biomarker - DNA-Barcoding von Fisch und Fischprodukten
anhand definierter mitochondrialer Cytochrom b- und Cytochrom c-Oxidase I-
Genabschnitte (ISO/DIS 17174:2023)
Analyse de biomarqueurs moléculaires - Codes-barres d’ADN de poissons et de produits
à base de poisson à l’aide de segments de gènes mitochondriaux de cytochrome b et
cytochrome c oxydase I (ISO/DIS 17174:2023)
Ta slovenski standard je istoveten z: prEN ISO 17174
ICS:
67.120.30 Ribe in ribji proizvodi Fish and fishery products
oSIST prEN ISO 17174:2023 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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oSIST prEN ISO 17174:2023

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oSIST prEN ISO 17174:2023
DRAFT INTERNATIONAL STANDARD
ISO/DIS 17174
ISO/TC 34/SC 16 Secretariat: ANSI
Voting begins on: Voting terminates on:
2023-05-15 2023-08-07
Molecular biomarker analysis — DNA barcoding of fish and
fish products using defined mitochondrial cytochrome b
and cytochrome c oxidase I gene segments
ICS: 67.120.30
This document is circulated as received from the committee secretariat.
THIS DOCUMENT IS A DRAFT CIRCULATED
FOR COMMENT AND APPROVAL. IT IS
ISO/CEN PARALLEL PROCESSING
THEREFORE SUBJECT TO CHANGE AND MAY
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NATIONAL REGULATIONS.
ISO/DIS 17174:2023(E)
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PROVIDE SUPPORTING DOCUMENTATION. © ISO 2023

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oSIST prEN ISO 17174:2023

DRAFT INTERNATIONAL STANDARD
ISO/DIS 17174
ISO/TC 34/SC 16 Secretariat: ANSI
Voting begins on: Voting terminates on:

Molecular biomarker analysis — DNA barcoding of fish and
fish products using defined mitochondrial cytochrome b
and cytochrome c oxidase I gene segments
ICS: 67.120.30
This document is circulated as received from the committee secretariat.
COPYRIGHT PROTECTED DOCUMENT
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PROVIDE SUPPORTING DOCUMENTATION. © ISO 2023

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oSIST prEN ISO 17174:2023
ISO/DIS 17174:2023(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope .
...