M/381 - Analysis of Foodstuffs

This standard describes the detection of pathogenic Vibrio parahaemolyticus and Vibrio cholerae (Reference document is ISO/TS 21872 -1)

This standard describes the detection and quantification of histamine

This part of the standard describes the detection of Campylobacter spp. (Reference document EN/ISO 10272 -1)

This standard describes the detection of Listeria monocytogenes (Reference document: EN/ISO 11290 -1 incl./Amd. 1)

This standard specifies an MPN method, with pre-enrichment, for the detection of Enterobacteriaceae. It is applicable to
- products intended for human consumption and the feeding of animals, and
- environmental samples in the areas of food production and food handling, and
- primary production samples.
(Reference document: ISO 21528-1)

This part of the standard describes the enumeration of Campylobacter spp. by means of colony count technique. (Reference document ISO/TS 10272 -2)

This part of EN ISO 21528 specifies a method, without pre-enrichment, for the enumeration of Enterobacteriaceae. It is applicable to - products intended for human consumption and the feeding of animals, and - environmental samples in the area of food production and food handling. Enumeration is carried out by counting colonies in a solid medium after incubation at 37 °C (or 30 °C).
This technique is recommended when the number of colonies sought is expected to be more than 100 per millilitre or per gram of the test sample.

This standard describes the enumeration of Listeria monocytogenes (Reference document: EN/ISO 11290 -2 incl./Amd. 1)

This standard describes the detection of staphylococcal enterotoxin for dairy products and other matrices

ISO 21872-1:2017 specifies a horizontal method for the detection of enteropathogenic Vibrio spp., which causes human illness in or via the intestinal tract. The species detectable by the methods specified include Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus.
ISO 21872-1:2017 is applicable to the following:
- products intended for human consumption and the feeding of animals;
- environmental samples in the area of food production and food handling.

ISO 21528-2:2017 specifies a method for the enumeration of Enterobacteriaceae. It is applicable to
- products intended for human consumption and the feeding of animals, and
- environmental samples in the area of primary production, food production and food handling.
This technique is intended to be used when the number of colonies sought is expected to be more than 100 per millilitre or per gram of the test sample.
The most probable number (MPN) technique, as included in ISO 21528‑1, is generally used when the number sought is expected to be below 100 per millilitre or per gram of test sample.

ISO 19343:2017 specifies a high performance liquid chromatography (HPLC) method to analyse histamine in fish and fishery products (fish sauces, fish maturated by enzyme in brine, etc.) intended for human consumption.

ISO 21528-1:2017 specifies a method, with enrichment, for the detection of Enterobacteriaceae. It is applicable to
- products intended for human consumption and the feeding of animals, and
- environmental samples in the area of primary production, food production and food handling.
This method is applicable
- when the microorganisms sought are expected to need resuscitation by enrichment, and
- when the number sought is expected to be below 100 per millilitre or per gram of test sample.
A limitation on the applicability of ISO 21528-1:2017 is imposed by the susceptibility of the method to a large degree of variability (see Clause 11).
NOTE Enumeration can be carried out by calculation of the most probable number (MPN) after incubation in liquid medium. See Annex A.

ISO 10272-1:2017 specifies a horizontal method for the detection by enrichment or direct plating of Campylobacter spp. It is applicable to
- products intended for human consumption,
- products intended for animal feeding,
- environmental samples in the area of food and feed production, handling, and
- samples from the primary production stage such as animal faeces, dust, and swabs.

ISO 10272-2:2017 specifies a horizontal method for the enumeration of Campylobacter spp. It is applicable to
- products intended for human consumption,
- products intended for animal feeding,
- environmental samples in the area of food and feed production, handling, and
- samples from the primary production stage such as animal faeces, dust, and swabs.

ISO 19020:2017 specifies a screening method for the detection of staphylococcal enterotoxins SEA, SEB, SECs, SED and SEE in foodstuffs. It consists of two main steps: a) extraction followed by a concentration based on dialysis principle; and b) an immunoenzymatic detection using commercially available detection kits.
ISO 19020:2017 is applicable to the screening of staphylococcal enterotoxins SEA to SEE in products intended for human consumption.
Other staphylococcal enterotoxins such as types SEG, SEH, SEI, SER, SES and SET can also cause illness. Due to the lack of commercially available detection kits, ISO 19020:2017 is applicable only to types SEA to SEE, but may apply to other types of toxins, subject to validation of the method.

ISO 11290-2:2017 specifies a horizontal method for
- the enumeration of L. monocytogenes, and
- the enumeration of Listeria spp. (including L. monocytogenes).
ISO 11290-2:2017 is applicable to
- products intended for human consumption and for the feeding of animals, and
- environmental samples in the area of food production and food handling.

ISO 11290-1:2017 specifies a horizontal method for
- the detection of L. monocytogenes, and
- the detection of Listeria spp. (including L. monocytogenes).
ISO 11290-1:2017 is applicable to
- products intended for human consumption and for the feeding of animals, and
- environmental samples in the area of food production and food handling.

This standard describes the detection of Enterobacter sakazakii  (Reference document: ISO/TS 22964)

This standard describes the detection of E.coli O157 (Reference document EN ISO 16654)

This standard describes the detection of Yersinia enterocolitica (Reference document (EN/ISO 10273)

This document specifies a method for the quantification of levels of HAV and norovirus genogroup I
(GI) and II (GII) RNA, from test samples of foodstuffs (soft fruit, leaf, stem and bulb vegetables, bottled
water, BMS) or food surfaces. Following liberation of viruses from the test sample, viral RNA is then
extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the
viral RNA are amplified and detected by real-time RT-PCR.
This method is not validated for detection of the target viruses in other foodstuffs (including multicomponent
foodstuffs), or any other matrices, nor for the detection of other viruses in foodstuffs, food
surfaces or other matrices.

ISO 10273:2017 specifies a horizontal method for the detection of Y. enterocolitica associated with human disease. It is applicable to
- products intended for human consumption and the feeding of animals, and
- environmental samples in the area of food production and food handling.

ISO 22964:2017 specifies a horizontal method for the detection of Cronobacter spp.
Subject to the limitations discussed in the introduction, this document is applicable to
- food products and ingredients intended for human consumption and the feeding of animals, and
- environmental samples in the area of food production and food handling.

This standard describes the detection of E.coli O157 (Reference document EN ISO 16654)

This document specifies a horizontal method for the detection of Salmonella. It is applicable to the
following:
— products intended for human consumption and the feeding of animals;
— environmental samples in the area of food production and food handling;
— samples from the primary production stage such as animal faeces, dust, and swabs.
With this horizontal method, most of the Salmonella serovars are intended to be detected. For the
detection of some specific serovars, additional culture steps may be needed. For Salmonella Typhi and
Salmonella Paratyphi, the procedure is described in Annex D.
The selective enrichment medium modified semi-solid Rappaport-Vassiliadis (MSRV) agar is intended
for the detection of motile Salmonella and is not appropriate for the detection of non-motile Salmonella
strains.

ISO 15216-1:2017 specifies a method for the quantification of levels of HAV and norovirus genogroup I (GI) and II (GII) RNA, from test samples of foodstuffs (soft fruit, leaf, stem and bulb vegetables, bottled water, BMS) or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR.
This method is not validated for detection of the target viruses in other foodstuffs (including multi-component foodstuffs), or any other matrices, nor for the detection of other viruses in foodstuffs, food surfaces or other matrices.

This document describes the quantitative analysis of the emetic toxin cereulide using high performance
liquid chromatography (HPLC) or ultra performance liquid chromatography (UHPLC) connected to a
tandem mass spectrometer (LC-MS/MS).
This document is applicable to the analysis of the toxin in products intended for human consumption.

ISO 6579-1:2017 specifies a horizontal method for the detection of Salmonella. It is applicable to the following:
- products intended for human consumption and the feeding of animals;
- environmental samples in the area of food production and food handling;
- samples from the primary production stage such as animal faeces, dust, and swabs.
With this horizontal method, most of the Salmonella serovars are intended to be detected. For the detection of some specific serovars, additional culture steps may be needed. For Salmonella Typhi and Salmonella Paratyphi, the procedure is described in Annex D.
The selective enrichment medium modified semi-solid Rappaport-Vassiliadis (MSRV) agar is intended for the detection of motile Salmonella and is not appropriate for the detection of non-motile Salmonella strains.

ISO 18465:2017 describes the quantitative analysis of the emetic toxin cereulide using high performance liquid chromatography (HPLC) or ultra performance liquid chromatography (UHPLC) connected to a tandem mass spectrometer (LC-MS/MS).
ISO 18465:2017 is applicable to the analysis of the toxin in products intended for human consumption.

This EN ISO Standard specifies the requirements and/or measures to eliminate the hazards or reduce the risks in the following groups of stationary grinding machines which are designed primarily to shape metal by grinding: Group 1: Manually controlled grinding machines without power operated axes and without numerical control Group 2: Manually controlled grinding machines with power operated axes and limited numerically controlled capability, if applicable Group 3: Numerically controlled grinding machines NOTE 1 For detailed information on the groups of grinding machines, see the definitions in 3.1 and 3.4. NOTE 2 Requirements in this EN ISO Standard are, in general, applicable to all groups of grinding machines. If requirements are applicable to some special group(s) of grinding machines only, then the special group(s) of grinding machine(s) is/are specified. NOTE 3 Hazards arising from other metalworking processes (e.g. turning and laser processing) are covered by other Standards

ISO 16089:2015 specifies the requirements and/or measures to eliminate the hazards or reduce the risks in the following groups of stationary grinding machines which are designed primarily to shape metal by grinding:
Group 1: Manually controlled grinding machines without power operated axes and without numerical control.
Group 2: Manually controlled grinding machines with power operated axes and limited numerically controlled capability, if applicable.
Group 3: Numerically controlled grinding machines.
NOTE 1 For detailed information on the groups of grinding machines, see the definitions in 3.1 and 3.4.
NOTE 2 Requirements in this International Standard are, in general, applicable to all groups of grinding machines. If requirements are applicable to some special group(s) of grinding machines only, then the special group(s) of grinding machine(s) is/are specified.
This International Standard covers the significant hazards listed in Clause 4 and applies to ancillary devices (e.g. for workpieces, tools, and workpiece holding devices, handling devices), which are integral to the machine.
This International Standard also applies to machines which are integrated into an automatic production line or grinding cell inasmuch as the hazards and risks arising are comparable to those of machines working separately.
This International Standard also includes in Clause 7 a minimum list of safety-relevant information which the manufacturer has to provide to the user. See also ISO 12100:2010, Figure 2, which illustrates the interaction of manufacturer's and user's responsibility for the operational safety.
The user's responsibility to identify specific hazards (e.g. fire and explosion) and reduce the associated risks can be critical (e.g. whether the central extraction system is working correctly).
Where additional metalworking processes (e.g. milling, turning, laser processing) are involved, this International Standard can be taken as a basis for safety requirements. For specific information on hazards arising from other metalworking processes, which are covered by other International Standards, see the Bibliography.
This International Standard applies to machines that are manufactured after the date of issue of this International Standard.
This International Standard does not apply to stationary honing, polishing, and belt grinding machines and not to transportable motor-operated electric tools in accordance with IEC 61029‑2‑4 and IEC 61029‑2‑10.