SIST EN 17710:2025
(Main)Plant biostimulants - Detection of Listeria monocytogenes
Plant biostimulants - Detection of Listeria monocytogenes
This document specifies a method for the detection of Listeria monocytogenes in microbial plant
biostimulants.
This document is applicable to the blends of fertilizing products where a blend is a mix of at least two of
the following component EU fertilising products categories: Fertilizers, Liming Materials, Soil
Improvers, Growing Media, Plant Biostimulants and where the following category Plant Biostimulants
is the highest percentage in the blend by mass or volume, or in the case of liquid form by dry mass. If
Plant Biostimulants is not the highest percentage in the blend, the European Standard for the highest
percentage of the blend applies. In case a blend of fertilizing products is composed of components in
equal quantity or in case the component EU fertilising products used for the blend have identical
formulations1, the user decides which standard to apply.
Pflanzen-Biostimulanzien - Nachweis von Listeria monocytogenes
Dieses Dokument enthält ein Verfahren zum Nachweis von Listeria monocytogenes in mikrobiellen Pflanzen-Biostimulanzien, um zu verifizieren, dass der Gehalt dieses Humanpathogens die entsprechenden Grenzwerte der EU Düngeprodukteverordnung[1] einhält.
Biostimulants des végétaux - Recherche de Listeria monocytogenes
Le présent document fournit une méthode pour la détection de Listeria monocytogenes dans les biostimulants microbiens des végétaux pour vérifier que la teneur de ce pathogène pour l’homme est conforme aux limites correspondantes énoncées dans le Règlement UE sur les fertilisants [1].
Rastlinski biostimulanti - Ugotavljanje prisotnosti Listeria monocytogenes
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-februar-2025
Nadomešča:
SIST-TS CEN/TS 17710:2023
Rastlinski biostimulanti - Ugotavljanje prisotnosti Listeria monocytogenes
Plant biostimulants - Detection of Listeria monocytogenes
Pflanzen-Biostimulanzien - Nachweis von Listeria monocytogenes
Biostimulants des végétaux - Recherche de Listeria monocytogenes
Ta slovenski standard je istoveten z: EN 17710:2024
ICS:
65.080 Gnojila Fertilizers
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN 17710
EUROPEAN STANDARD
NORME EUROPÉENNE
November 2024
EUROPÄISCHE NORM
ICS 65.080 Supersedes CEN/TS 17710:2022
English Version
Plant biostimulants - Detection of Listeria monocytogenes
Biostimulants des végétaux - Recherche de Listeria Pflanzen-Biostimulanzien - Nachweis von Listeria
monocytogenes monocytogenes
This European Standard was approved by CEN on 26 August 2024.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMA L I S A T IO N
EUROPÄISCHES KOMITEE FÜR NORMUN G
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2024 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 17710:2024 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle . 6
5 Culture media, reagents, antisera . 7
6 Equipment and consumables . 7
7 Sampling . 7
8 Preparation of test sample . 8
9 Expression of results . 13
10 Performance characteristics of the method . 13
11 Test report . 14
12 Quality assurance . 14
Annex A (normative) Diagram of the procedures . 15
Annex B (normative) Composition and preparation of culture media and reagents . 16
Annex C (informative) Method validation study and performance characteristics . 26
Annex ZA (informative) Relationship of this European Standard and the essential requirements
of Regulation (EU) 2019/1009 laying down rules on the making available on the market
of EU fertilising products aimed to be covered . 29
Bibliography . 30
European foreword
This document (EN 17710:2024) has been prepared by Technical Committee CEN/TC 455 “Plant
Biostimulants”, the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by May 2025, and conflicting national standards shall be
withdrawn at the latest by May 2025.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes CEN/TS 17710:2022.
CEN/TS 17710:2022:
— The European foreword and the introduction have been updated;
— The Normative references reported in Clause 2 have been updated;
— The definitions in Clause 3 have been revised;
— Clauses 8 and 9 have been combined and the text has been revised;
— Annexes A, B, and C have been revised;
— Annex ZA has been added.
This document has been prepared under a standardization request addressed to CEN by the European
Commission. The Standing Committee of the EFTA States subsequently approves these requests for its
Member States.
For the relationship with EU Legislation, see informative Annex ZA, which is an integral part of this
document.
Any feedback and questions on this document should be directed to the users’ national standards body.
A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organisations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia,
Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland,
Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of North
Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the United
Kingdom.
Introduction
The European Committee for Standardization (CEN) was requested by the European Commission (EC)
to draft European Standards or European Standardization deliverables to support the implementation
of Regulation (EU) 2019/1009 of 5 June 2019 [1] laying down rules on the making available on the
market of EU fertilising products (“FPR” or “Fertilising Products Regulation”).
This standardization request, presented as SR M/564 and relevant amendments, also contributes to the
Communication on “Innovating for Sustainable Growth: A Bio economy for Europe”.
The interest in plant biostimulants has increased significantly in Europe as a valuable tool to use in
agriculture. Standardization was identified as having an important role in order to promote the use of
biostimulants. The work of CEN/TC 455 seeks to improve the reliability of the supply chain, thereby
improving the confidence of farmers, industry, and consumers in biostimulants, and will promote and
support commercialisation of the European biostimulant industry.
WARNING — Persons using this document should be familiar with normal laboratory practice. This
document does not purport to address all of the safety problems, if any, associated with its use. It is the
responsibility of the user to establish appropriate safety and health practices and to ensure compliance
with any national regulatory conditions.
IMPORTANT — It is absolutely essential that tests conducted in accordance with this document be
carried out by suitably trained staff.
1 Scope
This document specifies a method for the detection of Listeria monocytogenes in microbial plant
biostimulants.
This document is applicable to the blends of fertilizing products where a blend is a mix of at least two of
the following component EU fertilising products categories: Fertilizers, Liming Materials, Soil
Improvers, Growing Media, Plant Biostimulants and where the following category Plant Biostimulants
is the highest percentage in the blend by mass or volume, or in the case of liquid form by dry mass. If
Plant Biostimulants is not the highest percentage in the blend, the European Standard for the highest
percentage of the blend applies. In case a blend of fertilizing products is composed of components in
equal quantity or in case the component EU fertilising products used for the blend have identical
formulations , the user decides which standard to apply.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
EN 17724:2024, Plant biostimulants — Terminology
EN 17708:2024, Plant biostimulants — Preparation of sample for microbial analysis
EN 17702-1:2024, Plant biostimulants — Sampling and sample preparation — Part 1: Sampling
EN ISO 7218:2024, Microbiology of food and animal feeding stuffs — General requirements and guidance
for microbiological examinations
EN ISO 11133:2014, Microbiology of food, animal feed and water — Preparation, production, storage and
performance testing of culture media
EN ISO 11290-1:2017, Microbiology of the food chain — Horizontal method for the detection and
enumeration of Listeria monocytogenes and of Listeria spp. — Part 1: Detection method
(ISO 11290-1:2017)
3 Terms and definitions
For the purposes of this document, the terms and definitions given in EN 17724:2024 and the following
apply.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— IEC Electropedia: available at https://www.electropedia.org/
— ISO Online browsing platform: available at https://www.iso.org/obp
An example of such a blend is a product with 2 claimed functions consisting of a non-microbial plant biostimulant
and an organic fertilizer composed of 1 kg/kg of plant biostimulant from seaweed.
As impacted by EN ISO 11133:2014/A1:2018 and EN ISO 11133:2014/A2:2020.
3.1
Listeria monocytogenes
microorganisms which form typical colonies on solid selective media and which display the
morphological, physiological and biochemical characteristics described when tests are carried out in
accordance with this document
Note 1 to entry: L. monocytogenes is a Gram-positive, non-spore forming, rod-shaped bacterium that belongs to
the genus Listeria, phylum Firmicutes.
Note 2 to entry: L. monocytogenes is a ubiquitous bacterial pathogen that causes serious localized and generalized
infections in humans.
Note 3 to entry: L. monocytogenes are catalase positive and oxidase negative. They produce flagella when grown
at a temperature between 20 °C and 25 °C but not at 37 °C. They produce a β-hemolysin on blood agar plates, which
is part of the CAMP (Christie, Atkins, and Munch-Petersen) diagnostic test.
Note 4 to entry: L. monocytogenes can grow at temperatures between −0,4 °C and 50 °C, with an optimum
temperature of 30–37 °C. They can withstand freezing, but they are inactivated by heating at 60 °C for 30 min.
Note 5 to entry: L. monocytogenes are facultative anaerobes that utilize glucose, lactose, and rhamnose under
aerobic conditions and can ferment several hexoses and pentoses under anaerobic conditions.
Note 6 to entry: L. monocytogenes can grow over a pH range of 4–9.5 and a water activity of 0.90 to 0.97. They can
also grow in 10 % sodium chloride.
[SOURCE: EN ISO 11290-1:2017, 3.1]
4 Principle
4.1 General
The detection of Listeria monocytogenes requires four successive stages as specified in Annex A.
NOTE L. monocytogenes can be present in small numbers and is often accompanied by a considerably larger
number of bacteria belonging to different taxonomic groups or different Listeria species. Pre-enrichment is used
to permit the detection of low numbers of L. monocytogenes or injured L. monocytogenes [2].
4.2 Pre-enrichment in selective liquid medium
Half-Fraser broth (225 ml) at ambient temperature shall be inoculated with the test portion (25 g or
25 ml), then shall be incubated at 30 °C ± 2 °C for 24 h to 26 h.
For large quantities (e.g. 1 l or more), it is recommended to pre-warm the broth to 30 °C before mixing
it with the test portion.
4.3 Enrichment in/on selective media
Fraser broth shall be inoculated at 37 °C (0,1 ml of culture in 10 ml of Fraser broth) and shall be
incubated at 37 °C ± 2 °C for 24 h ± 2 h.
4.4 Plating out on selective solid media
Both primary and secondary enrichments shall be streaked onto:
— Agar Listeria a
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