Soil quality - Guidance on laboratory testing for biodegradation of organic chemicals in soil under anaerobic conditions (ISO 15473:2002)

This International Standard gives guidance on the selection and method of appropriate tests for the determination of biodegradation of organic chemicals in soil samples under anaerobic conditions.

Bodenbeschaffenheit - Anleitung für Laboratoriumsuntersuchungen zur biologischen Abbaubarkeit von organischen Chemikalien im Boden unter anaeroben Bedingungen (ISO 15473:2002)

Diese Internationale Norm gibt eine Anleitung für die Auswahl und Durchführung geeigneter Prüfverfahren zur Bestimmung der biologischen Abbaubarkeit von organischen Chemikalien in Bodenproben unter anaeroben Bedingungen.
ANMERKUNG   Falls das Verfahren für Prüfungen im Netzwerk der Registrierung von Chemikalien vorgesehen ist, enthält eine OECD-Anleitung zur Abbaubarkeit im Boden [20] nützliche Hinweise über zusätzliche Prüfanforderungen.

Qualité du sol - Lignes directrices relatives aux essais en laboratoire pour la biodégradation de produits chimiques organiques dans le sol sous conditions anaérobies (ISO 15473:2002)

Kakovost tal - Navodilo za laboratorijsko preskušanje biološke razgradljivosti organskih spojin v tleh pri anaerobnih pogojih (ISO 15473:2002)

General Information

Status
Published
Public Enquiry End Date
02-Feb-2020
Publication Date
23-Sep-2020
Technical Committee
Current Stage
6060 - National Implementation/Publication (Adopted Project)
Start Date
17-Aug-2020
Due Date
22-Oct-2020
Completion Date
24-Sep-2020

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SLOVENSKI STANDARD
SIST EN ISO 15473:2020
01-november-2020
Kakovost tal - Navodilo za laboratorijsko preskušanje biološke razgradljivosti
organskih spojin v tleh pri anaerobnih pogojih (ISO 15473:2002)

Soil quality - Guidance on laboratory testing for biodegradation of organic chemicals in

soil under anaerobic conditions (ISO 15473:2002)
Bodenbeschaffenheit - Anleitung für Laboratoriumsuntersuchungen zur biologischen
Abbaubarkeit von organischen Chemikalien im Boden unter anaeroben Bedingungen
(ISO 15473:2002)
Qualité du sol - Lignes directrices relatives aux essais en laboratoire pour la

biodégradation de produits chimiques organiques dans le sol sous conditions anaérobies

(ISO 15473:2002)
Ta slovenski standard je istoveten z: EN ISO 15473:2020
ICS:
13.080.30 Biološke lastnosti tal Biological properties of soils
SIST EN ISO 15473:2020 en,fr,de

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN ISO 15473:2020
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SIST EN ISO 15473:2020
EN ISO 15473
EUROPEAN STANDARD
NORME EUROPÉENNE
April 2020
EUROPÄISCHE NORM
ICS 13.080.30
English Version
Soil quality - Guidance on laboratory testing for
biodegradation of organic chemicals in soil under
anaerobic conditions (ISO 15473:2002)

Qualité du sol - Lignes directrices relatives aux essais Bodenbeschaffenheit - Anleitung für

en laboratoire pour la biodégradation de produits Laboratoriumsuntersuchungen zur biologischen

chimiques organiques dans le sol sous conditions Abbaubarkeit von organischen Chemikalien im Boden

anaérobies (ISO 15473:2002) unter anaeroben Bedingungen (ISO 15473:2002)
This European Standard was approved by CEN on 13 April 2020.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this

European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references

concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN

member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by

translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management

Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,

Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,

Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and

United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels

© 2020 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 15473:2020 E

worldwide for CEN national Members.
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SIST EN ISO 15473:2020
EN ISO 15473:2020 (E)
Contents Page

European foreword ....................................................................................................................................................... 3

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SIST EN ISO 15473:2020
EN ISO 15473:2020 (E)
European foreword

The text of ISO 15473:2002 has been prepared by Technical Committee ISO/TC 190 "Soil quality” of the

International Organization for Standardization (ISO) and has been taken over as EN ISO 15473:2020 by

Technical Committee CEN/TC 444 “Environmental characterization of solid matrices” the secretariat of

which is held by NEN.

This European Standard shall be given the status of a national standard, either by publication of an

identical text or by endorsement, at the latest by October 2020, and conflicting national standards shall

be withdrawn at the latest by October 2020.

Attention is drawn to the possibility that some of the elements of this document may be the subject of

patent rights. CEN shall not be held responsible for identifying any or all such patent rights.

According to the CEN-CENELEC Internal Regulations, the national standards organizations of the

following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,

Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,

Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of

North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the

United Kingdom.
Endorsement notice

The text of ISO 15473:2002 has been approved by CEN as EN ISO 15473:2020 without any modification.

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SIST EN ISO 15473:2020
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SIST EN ISO 15473:2020
INTERNATIONAL ISO
STANDARD 15473
First edition
2002-03-15
Soil quality — Guidance on laboratory
testing for biodegradation of organic
chemicals in soil under anaerobic
conditions
Qualité du sol — Lignes directrices relatives aux essais en laboratoire pour
la biodégradation de produits chimiques organiques dans le sol sous
conditions anaérobies
Reference number
ISO 15473:2002(E)
ISO 2002
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SIST EN ISO 15473:2020
ISO 15473:2002(E)
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ii © ISO 2002 – All rights reserved
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SIST EN ISO 15473:2020
ISO 15473:2002(E)
Contents

Foreword.....................................................................................................................................................................iv

Introduction.................................................................................................................................................................v

1 Scope..............................................................................................................................................................1

2 Normative references....................................................................................................................................1

3 Terms and definitions ...................................................................................................................................1

4 Principle..........................................................................................................................................................3

5 Materials.........................................................................................................................................................3

6 Collection, handling and storage of soil .....................................................................................................6

7 Procedure.......................................................................................................................................................6

8 Expression of results....................................................................................................................................9

9 Test report....................................................................................................................................................10

Bibliography..............................................................................................................................................................11

© ISO 2002 – All rights reserved iii
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SIST EN ISO 15473:2020
ISO 15473:2002(E)
Foreword

ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO

member bodies). The work of preparing International Standards is normally carried out through ISO technical

committees. Each member body interested in a subject for which a technical committee has been established has

the right to be represented on that committee. International organizations, governmental and non-governmental, in

liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical

Commission (IEC) on all matters of electrotechnical standardization.

International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 3.

The main task of technical committees is to prepare International Standards. Draft International Standards adopted

by the technical committees are circulated to the member bodies for voting. Publication as an International

Standard requires approval by at least 75 % of the member bodies casting a vote.

Attention is drawn to the possibility that some of the elements of this International Standard may be the subject of

patent rights. ISO shall not be held responsible for identifying any or all such patent rights.

ISO 15473 was prepared by Technical Committee ISO/TC 190, Soil quality, Subcommittee SC 4, Biological

methods.
iv © ISO 2002 – All rights reserved
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SIST EN ISO 15473:2020
ISO 15473:2002(E)
Introduction

Organic chemicals can be introduced into the soil both intentionally and accidentally, after which they can degrade

as a result of biological action. For chemicals which do degrade, the rate of degradation can vary considerably,

depending not only on the molecular structure of the chemical, but also on soil conditions such as temperature,

water and oxygen availability which influence microbial activity. The activity of microorganisms often plays a major

role in degradative processes.

ISO 11266 [3] gives general guidelines for the selection and method of tests to determine the biodegradation of

organic chemicals in soils under aerobic conditions.

It is necessary to have laboratory tests available to estimate the rate and extent of biodegradation under anaerobic

conditions, and to assess the capability of soil to degrade organic chemicals under these conditions.

This International Standard gives guidance for the method of tests to determine the biodegradation of organic

chemicals in soils under anaerobic conditions.
© ISO 2002 – All rights reserved v
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SIST EN ISO 15473:2020
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SIST EN ISO 15473:2020
INTERNATIONAL STANDARD ISO 15473:2002(E)
Soil quality — Guidance on laboratory testing for biodegradation
of organic chemicals in soil under anaerobic conditions
1 Scope

This International Standard gives guidance on the selection and method of appropriate tests for the determination

of biodegradation of organic chemicals in soil samples under anaerobic conditions.

NOTE If the method is intended for tests in the framework of the registration of chemicals, an OECD Guideline on soil

degradation [20] gives useful guidance on additional test requirements.
2 Normative references

The following normative documents contain provisions which, through reference in this text, constitute provisions of

this International Standard. For dated references, subsequent amendments to, or revisions of, any of these

publications do not apply. However, parties to agreements based on this International Standard are encouraged to

investigate the possibility of applying the most recent editions of the normative documents indicated below. For

undated references, the latest edition of the normative document referred to applies. Members of ISO and IEC

maintain registers of currently valid International Standards.

ISO 10381-6:1993, Soil quality — Sampling — Part 6: Guidance on the collection, handling and storage of soil for

the assessment of aerobic microbial processes in the laboratory
ISO 10390:1994, Soil quality — Determination of pH

ISO 10694:1995, Soil quality — Determination of organic and total carbon after dry combustion (elementary

analysis)

ISO 11260:1994, Soil quality — Determination of effective cation exchange capacity and base saturation level

using barium chloride solution

ISO 11261:1995, Soil quality — Determination of total nitrogen — Modified Kjeldahl method

ISO 11271, Soil quality — Determination of redox potential — Field method

ISO 11274:1998, Soil quality — Determination of the water retention characteristic — Laboratory methods

ISO 11277:1998, Soil quality — Determination of particle size distribution in mineral soil material — Method by

sieving and sedimentation

ISO 14239:1997, Soil quality — Laboratory incubation systems for measuring the mineralization of organic

chemicals in soil under aerobic conditions
3 Terms and definitions

For the purposes of this International Standard, the following terms and definitions apply.

© ISO 2002 – All rights reserved 1
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SIST EN ISO 15473:2020
ISO 15473:2002(E)
3.1
biodegradation

molecular degradation of an organic substance resulting from the actions of living organisms

[ISO 11266]
3.2
primary biodegradation

the degradation of a substance to an extent sufficient to remove some characteristic property of the parent

molecule. In practice this will be determined by analysis as a loss of parent compound or some specific function of

the parent compound
[ISO 11266]
3.3
ultimate biodegradation

breakdown of an organic compound to carbon dioxide, methane, water, mineral salts of any other elements

present, and products associated with the normal anaerobic processes of microorganisms

3.4
anaerobic transformation
reaction occurring under exclusion of oxygen (reducing conditions)

NOTE Such a reaction generally occurs when the redox potential (E ) is less than 200 mV [17].

3.5
persistence

residence time of a chemical species in a specifically defined compartment of the environment

[ISO 11266]
3.6
DT-50
disappearance time 50

time taken for the concentration of a given compound to be reduced by 50 % of its original value

[ISO 11266]
3.7
DT-90
disappearance time 90

time taken for the concentration of a given compound to be reduced by 90 % of its original value

[ISO 11266]
3.8
bound residue
non-extractable residue

chemical species in soils originating from, for example, organic molecules that are not extracted by methods which

do not significantly change the chemical nature of the residue

NOTE These non-extractable residues are considered to exclude fragments recycled through metabolic pathways leading

to natural products [12].
3.9
soil

upper layer of the earth's crust composed of mineral parts, organic substances, water, air and living matter

[ISO 11074-1]
2 © ISO 2002 – All rights reserved
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SIST EN ISO 15473:2020
ISO 15473:2002(E)
3.10
test substance
chemical substance under investigation added to the test system
3.11
saturated soil
that part of the soil which is completely saturated by water
4 Principle
Two appropriate test methods are described:

a) incubation of a test compound in the soil under methanogenic conditions and monitoring its biodegradation;

b) incubation of a test compound in the soil under water-logged conditions and monitoring its biodegradation.

The latter method simulates conditions under natural anaerobic circumstances, whereas the former method makes

use of chemicals to induce a low redox potential in soil, and is the method of choice to measure the potential for

degradation in soil under methanogenic conditions. In the water-logged soil method, the establishment of a low

redox potential takes more time than under the methanogenic test conditions.

If the water-logged (“flooded”) conditions are chosen, the soil will establish conditions depending on the nature of

the soil. Such conditions can be nitrate-reducing (450 mV to 200 mV, pH 7), Fe-reducing (+ 150 mV to – 100 mV,

pH 7), or sulfate-reducing (– 50 mV to – 200 mV, pH 7). If “methanogenic” conditions are chosen, the redox

potential will be less than – 200 mV.

The water-logging method is more appropriate for aerobic soils that may be transiently anaerobic. The

methanogenic conditions are more appropriate for organic marsh (permanently flooded) surface soils, soils of

landfills and sludge-amended soils.

NOTE Organic soils containing easily degradable organic matter may eventually achieve methanogenic conditions under

water-logged test conditions.

After addition of the test compound to a selected soil (5.1), biodegradation is measured under anaerobic conditions

by following the production of carbon dioxide, methane and other volatiles. If such volatile compounds have to be

determined, the use of C (radioactive) substances is highly recommended. The disappearance of the test

compound can also be f
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