SIST EN ISO 23611-4:2023

Soil quality - Sampling of soil invertebrates - Part 4: Sampling, extraction and identification of soil-inhabiting nematodes (ISO 23611-4:2022)

This document specifies a method for sampling and handling free-living nematodes from terrestrial field soils as a prerequisite for using them as bio-indicators (e.g. to assess the quality of a soil as a habitat for organisms).
This document applies to all terrestrial biotopes in which nematodes occur. The sampling design of field studies in general is specified in ISO 18400-101.
This document is not applicable to aquatic nematodes because of differences in the sample matrix (e.g. water column). Methods for some other soil organism groups such as earthworms, collembolans enchytraeids or macro-invertebrates are covered in ISO 23611-1, ISO 23611-2, ISO 23611-3 and ISO 23611-5.
This document does not cover the pedological characterization of the site which is highly recommendable when sampling soil invertebrates. ISO 10390, ISO 10694, ISO 11272, ISO 11274, ISO 11277, ISO 11461 and ISO 11465 include suitable procedures for measuring pH, particle size distribution, C/N ratio, organic carbon content and water-holding capacity.

Bodenbeschaffenheit - Probenahme von Wirbellosen im Boden - Teil 4: Probenahme, Extraktion und Bestimmung von Boden bewohnenden Nematoden (ISO 23611-4:2022)

Dieses Dokument legt ein Verfahren zur Probenahme und Handhabung frei lebender Nematoden aus terrestrischen Freilandböden fest, das als eine Voraussetzung für die Verwendung dieser Tiere als Bioindikatoren (z. B. zur Beurteilung der Beschaffenheit eines Bodens als Lebensraum für Organismen) dient.
Dieses Dokument wird angewendet für alle terrestrischen Biotope, in denen Nematoden vorkommen. Die allgemeine Planung der Probenahme für Freilanduntersuchungen ist in ISO 18400 101 festgelegt.
Dieses Dokument ist wegen der Unterschiede in der Probenmatrix (z. B. Wassersäule) nicht auf aquatische Nematoden anwendbar. Methoden für einige andere Gruppen von Bodenorganismen wie Regenwürmer, Collembolen, Enchytraeiden oder Makroinvertebraten werden in ISO 23611 1, ISO 23611 2, ISO 23611 3 und ISO 23611 5 behandelt.
Dieses Dokument behandelt nicht die Charakterisierung des Standortes nach bodenkundlichen Gesichtspunkten, die für die Probenahme von Wirbellosen im Boden nachdrücklich empfohlen wird.ISO 10390, ISO 10694, ISO 11272, ISO 11274, ISO 11277, ISO 11461 und ISO 11465 behandeln zur Bestimmung des pH Wertes, der Korngrößenverteilung, des C/N Verhältnisses, des Gehaltes an organischem Kohlenstoff und des Wasserrückhaltevermögens geeignetere Verfahren.

Qualité du sol - Prélèvement des invertébrés du sol - Partie 4 : Prélèvement, extraction et identification des nématodes du sol (ISO 23611-4:2022)

Le présent document spécifie une méthode pour échantillonner et manipuler les nématodes du sol en tant que condition préalable pour les utiliser comme bio-indicateurs (par exemple pour évaluer la qualité d’un sol en tant qu’habitat pour des organismes).
Le présent document s’applique à tous les biotopes terrestres dans lesquels des nématodes sont présents. Le mode opératoire d’échantillonnage pour les études de terrain est spécifié de façon générale dans l’ISO 18400-101.
Le présent document n’est pas applicable aux nématodes aquatiques en raison des différences dans la matrice d’échantillonnage (par exemple, la colonne d’eau). Des méthodes destinées à certains autres groupes d’organismes tels que les vers de terre, les collemboles, les enchytréides ou les macro-invertébrés, sont décrites dans l’ISO 23611-1, l’ISO 23611-2, l’ISO 23611-3 et l’ISO 23611-5.
Le présent document ne couvre pas la caractérisation pédologique du site qui est particulièrement recommandée lors du prélèvement d’invertébrés du sol. L’ISO 10390, l’ISO 10694, l’ISO 11272, l’ISO 11274, l’ISO 11277, l’ISO 11461 et l’ISO 11465 décrivent des modes opératoires appropriés pour mesurer le pH, la répartition granulométrique, le rapport C/N, la teneur en carbone organique et la capacité de rétention en eau.

Kakovost tal - Vzorčenje nevretenčarjev v tleh - 4. del: Vzorčenje, ekstrakcija in identifikacija nematod iz tal (ISO 23611-4:2022)

Ta dokument določa metodo za vzorčenje prostoživečih nematodov v tleh ter rokovanje z njimi kot predpogoj za uporabo teh organizmov kot bioloških indikatorjev (npr. za oceno kakovosti tal kot habitata za organizme).
Ta dokument se uporablja za vse kopenske biotope, v katerih se pojavljajo nematodi. Načrtovanje vzorčenja za terenske raziskave na splošno je podano v standardu ISO 18400-101.
Ta dokument se ne uporablja za vodne nematode zaradi razlik v matrici vzorca (npr. vodni stolpec). Metode za nekatere druge skupine talnih organizmov, kot so deževniki, drobne žuželke ali makro nevretenčarji, so podane v standardih ISO 23611-1, ISO 23611-2, ISO 23611-3 in ISO 23611-5.
Ta dokument ne obravnava pedološke karakterizacije lokacije, ki je zelo priporočljiva pri vzorčenju talnih nevretenčarjev. Za merjenje vrednosti pH, porazdelitve velikosti delcev, razmerja C/N, vsebnosti organskega ogljika in zmogljivosti zadrževanja vode so primernejši postopki v standardih ISO 10390, ISO 10694, ISO 11272, ISO 11274, ISO 11277, ISO 11461 in ISO 11465.

General Information

• Status: Published
• Public Enquiry End Date: 01-Dec-2021
• Publication Date: 12-Feb-2023
• ICS: 13.080.05 - Examination of soils in general
• ICS: 13.080.30 - Biological properties of soils
• Technical Committee: KAT - Soil quality
• Current Stage: 6060 - National Implementation/Publication (Adopted Project)
• Start Date: 12-Jan-2023
• Due Date: 19-Mar-2023
• Completion Date: 13-Feb-2023
• Ref Project: EN ISO 23611-4:2022 - Soil quality - Sampling of soil invertebrates - Part 4: Sampling, extraction and identification of soil-inhabiting nematodes (ISO 23611-4:2022)

Relations

• Replaces: SIST EN ISO 23611-4:2012 - Soil quality - Sampling of soil invertebrates - Part 4: Sampling, extraction and identification of soil-inhabiting nematodes (ISO 23611-4:2007)
• Effective Date: 01-Mar-2023

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN ISO 23611-4:2023
01-marec-2023
Nadomešča:
SIST EN ISO 23611-4:2012
Kakovost tal - Vzorčenje nevretenčarjev v tleh - 4. del: Vzorčenje, ekstrakcija in
identifikacija nematod iz tal (ISO 23611-4:2022)
Soil quality - Sampling of soil invertebrates - Part 4: Sampling, extraction and
identification of soil-inhabiting nematodes (ISO 23611-4:2022)
Bodenbeschaffenheit - Probenahme von Wirbellosen im Boden - Teil 4: Probenahme,
Extraktion und Bestimmung von Boden bewohnenden Nematoden (ISO 23611-4:2022)
Qualité du sol - Prélèvement des invertébrés du sol - Partie 4 : Prélèvement, extraction
et identification des nématodes du sol (ISO 23611-4:2022)
Ta slovenski standard je istoveten z: EN ISO 23611-4:2022
ICS:
13.080.30 Biološke lastnosti tal Biological properties of soils
SIST EN ISO 23611-4:2023 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN ISO 23611-4:2023

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SIST EN ISO 23611-4:2023


EN ISO 23611-4
EUROPEAN STANDARD

NORME EUROPÉENNE

August 2022
EUROPÄISCHE NORM
ICS 13.080.30; 13.080.05 Supersedes EN ISO 23611-4:2011
English Version

Soil quality - Sampling of soil invertebrates - Part 4:
Sampling, extraction and identification of soil-inhabiting
nematodes (ISO 23611-4:2022)
Qualité du sol - Prélèvement des invertébrés du sol - Bodenbeschaffenheit - Probenahme von Wirbellosen
Partie 4 : Prélèvement, extraction et identification des im Boden - Teil 4: Probenahme, Extraktion und
nématodes du sol (ISO 23611-4:2022) Bestimmung von Boden bewohnenden Nematoden
(ISO 23611 4:2022)
This European Standard was approved by CEN on 16 July 2022.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2022 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 23611-4:2022 E
worldwide for CEN national Members.

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SIST EN ISO 23611-4:2023
EN ISO 23611-4:2022 (E)
Contents Page
European foreword . 3

2

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SIST EN ISO 23611-4:2023
EN ISO 23611-4:2022 (E)
European foreword
This document (EN ISO 23611-4:2022) has been prepared by Technical Committee ISO/TC 190 "Soil
quality" in collaboration with Technical Committee CEN/TC 444 “Environmental characterization of
solid matrices” the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by February 2023, and conflicting national standards
shall be withdrawn at the latest by February 2023.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 23611-4:2011.
Any feedback and questions on this document should be directed to the users’ national standards
body/national committee. A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
No
...

SLOVENSKI STANDARD
oSIST prEN ISO 23611-4:2021
01-november-2021
Kakovost tal - Vzorčenje nevretenčarjev v tleh - 4. del: Vzorčenje, ekstrakcija in
identifikacija nematod iz tal (ISO/DIS 23611-4:2021)
Soil quality - Sampling of soil invertebrates - Part 4: Sampling, extraction and
identification of soil-inhabiting nematodes (ISO/DIS 23611-4:2021)
Bodenbeschaffenheit - Probenahme von Wirbellosen im Boden - Teil 4: Probenahme,
Extraktion und Bestimmung von Boden bewohnenden Nematoden (ISO/DIS 23611
4:2021)
Qualité du sol - Prélèvement des invertébrés du sol - Partie 4 : Prélèvement, extraction
et identification des nématodes du sol (ISO/DIS 23611-4:2021)
Ta slovenski standard je istoveten z: prEN ISO 23611-4
ICS:
13.080.30 Biološke lastnosti tal Biological properties of soils
oSIST prEN ISO 23611-4:2021 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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oSIST prEN ISO 23611-4:2021

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oSIST prEN ISO 23611-4:2021
DRAFT INTERNATIONAL STANDARD
ISO/DIS 23611-4
ISO/TC 190/SC 4 Secretariat: AFNOR
Voting begins on: Voting terminates on:
2021-10-04 2021-12-27
Soil quality — Sampling of soil invertebrates —
Part 4:
Sampling, extraction and identification of soil-inhabiting
nematodes
Qualité du sol — Prélèvement des invertébrés du sol —
Partie 4: Prélèvement, extraction et identification des nématodes du sol
ICS: 13.080.05; 13.080.30
THIS DOCUMENT IS A DRAFT CIRCULATED
This document is circulated as received from the committee secretariat.
FOR COMMENT AND APPROVAL. IT IS
THEREFORE SUBJECT TO CHANGE AND MAY
NOT BE REFERRED TO AS AN INTERNATIONAL
STANDARD UNTIL PUBLISHED AS SUCH.
IN ADDITION TO THEIR EVALUATION AS
ISO/CEN PARALLEL PROCESSING
BEING ACCEPTABLE FOR INDUSTRIAL,
TECHNOLOGICAL, COMMERCIAL AND
USER PURPOSES, DRAFT INTERNATIONAL
STANDARDS MAY ON OCCASION HAVE TO
BE CONSIDERED IN THE LIGHT OF THEIR
POTENTIAL TO BECOME STANDARDS TO
WHICH REFERENCE MAY BE MADE IN
Reference number
NATIONAL REGULATIONS.
ISO/DIS 23611-4:2021(E)
RECIPIENTS OF THIS DRAFT ARE INVITED
TO SUBMIT, WITH THEIR COMMENTS,
NOTIFICATION OF ANY RELEVANT PATENT
RIGHTS OF WHICH THEY ARE AWARE AND TO
©
PROVIDE SUPPORTING DOCUMENTATION. ISO 2021

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oSIST prEN ISO 23611-4:2021
ISO/DIS 23611-4:2021(E)

COPYRIGHT PROTECTED DOCUMENT
© ISO 2021
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
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Published in Switzerland
ii © ISO 2021 – All rights reserved

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oSIST prEN ISO 23611-4:2021
ISO/DIS 23611-4:2021(E)

Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Reagents . 3
6 Apparatus .
...