SIST EN ISO 18187:2024
(Main)Soil quality - Contact test for solid samples using the dehydrogenase activity of Arthrobacter globiformis (ISO 18187:2024)
Soil quality - Contact test for solid samples using the dehydrogenase activity of Arthrobacter globiformis (ISO 18187:2024)
ISO 18187:2016 specifies a rapid method for assessing solid samples in an aerobic suspension, by determining the inhibition of dehydrogenase activity of Arthrobacter globiformis using the redox dye resazurin.
It is applicable for assessing the effect of water-soluble and solid matter bounded non-volatile contaminants of natural samples, such as soils and waste materials. The test yields a result within 6 h and can therefore be used for screening potentially contaminated material.
Bodenbeschaffenheit - Feststoffkontakttest unter Verwendung der Dehydrogenaseaktivität von Arthrobacter globiformis (ISO 18187:2024)
Dieses Dokument legt ein Schnellverfahren zur Beurteilung von Feststoffproben in einer aeroben Suspension durch Bestimmung der Hemmung der Dehydrogenaseaktivität von Arthrobacter globiformis unter Verwendung des Redox-Farbstoffes Resazurin fest.
Sie gilt für die Beurteilung der Auswirkung von wasserlöslichen und feststoffgebundenen nicht flüchtigen Verunreinigungen in natürlichen Proben, wie Böden und Abfällen. Obwohl dies nicht der Hauptzweck ist, kann der Kontakttest auch zur Prüfung der Wirkung von Chemikalien verwendet werden, wie in Anhang C beschrieben. Die Prüfung führt innerhalb von 6 h zu einem Ergebnis und kann deshalb zum Screening potentiell verunreinigten Test-Materials eingesetzt werden.
Qualité du sol - Essai contact pour échantillons solides utilisant l'activité déshydrogénase de Arthrobacter globiformis (ISO 18187:2024)
ISO 18187:2016 spécifie une méthode rapide d'évaluation d'échantillons solides en suspension aérobie, en déterminant l'inhibition de l'activité déshydrogénase de Arthrobacter globiformis à l'aide d'un indicateur redox coloré, la résazurine.
Cette méthode est applicable à l'évaluation de l'effet de contaminants non volatils, liés aux matières solides ou solubles dans l'eau, présents dans des échantillons naturels tels que des sols et des déchets. L'essai délivre un résultat en 6 h et peut donc être utilisé pour le criblage de matériaux potentiellement contaminés.
Kakovost tal - Kontaktni preskus za trdne vzorce z dehidrogenazno aktivnostjo Arthrobacter globiformis (ISO 18187:2024)
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Standards Content (Sample)
SLOVENSKI STANDARD
oSIST prEN ISO 18187:2023
01-september-2023
Kakovost tal - Kontaktni preskus za trdne vzorce z dehidrogenazno aktivnostjo
Arthrobacter globiformis (ISO/DIS 18187:2023)
Soil quality - Contact test for solid samples using the dehydrogenase activity of
Arthrobacter globiformis (ISO/DIS 18187:2023)
Bodenbeschaffenheit - Feststoffkontakttest unter Verwendung der
Dehydrogenaseaktivität von Arthrobacter globiformis (ISO/DIS 18187:2023)
Qualité du sol - Essai contact pour échantillons solides utilisant l'activité déshydrogénase
de Arthrobacter globiformis (ISO/DIS 18187:2023)
Ta slovenski standard je istoveten z: prEN ISO 18187
ICS:
13.080.30 Biološke lastnosti tal Biological properties of soils
oSIST prEN ISO 18187:2023 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
oSIST prEN ISO 18187:2023
oSIST prEN ISO 18187:2023
DRAFT INTERNATIONAL STANDARD
ISO/DIS 18187
ISO/TC 190/SC 4 Secretariat: AFNOR
Voting begins on: Voting terminates on:
2023-06-29 2023-09-21
Soil quality — Contact test for solid samples using the
dehydrogenase activity of Arthrobacter globiformis
Qualité du sol — Essai contact pour échantillons solides utilisant l'activité déshydrogénase de Arthrobacter
globiformis
ICS: 13.080.30
This document is circulated as received from the committee secretariat.
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FOR COMMENT AND APPROVAL. IT IS
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THEREFORE SUBJECT TO CHANGE AND MAY
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NATIONAL REGULATIONS.
ISO/DIS 18187:2023(E)
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PROVIDE SUPPORTING DOCUMENTATION. © ISO 2023
oSIST prEN ISO 18187:2023
ISO/DIS 18187:2023(E)
DRAFT INTERNATIONAL STANDARD
ISO/DIS 18187
ISO/TC 190/SC 4 Secretariat: AFNOR
Voting begins on: Voting terminates on:
Soil quality — Contact test for solid samples using the
dehydrogenase activity of Arthrobacter globiformis
Qualité du sol — Essai contact pour échantillons solides utilisant l'activité déshydrogénase de Arthrobacter
globiformis
ICS: 13.080.30
This document is circulated as received from the committee secretariat.
THIS DOCUMENT IS A DRAFT CIRCULATED
FOR COMMENT AND APPROVAL. IT IS
© ISO 2023
ISO/CEN PARALLEL PROCESSING
THEREFORE SUBJECT TO CHANGE AND MAY
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
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NATIONAL REGULATIONS.
Website: www.iso.org ISO/DIS 18187:2023(E)
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ii
PROVIDE SUPPORTING DOCUMENTATION. © ISO 2023
oSIST prEN ISO 18187:2023
ISO/DIS 18187:2023(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 4
5 Reagents and material . 4
5.1 Test organisms . 4
5.2 Control substrates . 4
5.2.1 General . 4
5.2.2 Control for soils . 5
5.2.3 Control for waste material . 5
5.3 Test substrates . . . 5
5.4 Chemicals . 6
6 Apparatus . 8
7 Procedure .9
7.1 Preparation of dilutions . 9
7.2 Reference substance and positive control preparation . 9
7.3 Contact test procedure . 10
7.3.1 General . 10
7.3.2 Aeration . 11
7.3.3 Deactivation . 11
7.3.4 Preparation of the inoculum . 11
7.3.5 Incubation and fluorescence measurement . 11
7.4 Interferences .12
8 Calculation and expression of results .12
8.1 Calculation .12
8.1.1 Relative fluorescence .12
8.1.2 Determining the percentage of inhibition .12
8.2 Expression of results . .13
9 Validity of the test .13
10 Statistical analysis .14
11 Test report .14
Annex A (informative) Results on the ring test .16
Annex B (informative) Preparation of test organisms .23
Annex C (informative) Testing chemical substances .25
Bibliography .27
iii
oSIST prEN ISO 18187:2023
ISO/DIS 18187:2023(E)
Foreword
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This document was prepared by Technical Committee ISO/TC 190, Soil quality, Subcommittee SC 4,
Biological methods.
This second/third/… edition cancels and replaces the first/second/… edition (ISO #####:####), which
has been technically revised.
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iv
oSIST prEN ISO 18187:2023
ISO/DIS 18187:2023(E)
Introduction
This International Standard describes the miniaturized solid contact assay with Arthrobacter globiformis
that allows the preliminary assessment of solid material (i.e. soil and waste materials) within 6 h. The
principle of the assay relies on dehydrogenase activity inhibition of an added test organism, caused by
bioavailable toxic substances in soil and waste samples. This is an ecologically releva
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