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SIST EN ISO 18187:2024

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Soil quality - Contact test for solid samples using the dehydrogenase activity of Arthrobacter globiformis (ISO 18187:2024)

Soil quality - Contact test for solid samples using the dehydrogenase activity of Arthrobacter globiformis (ISO 18187:2024)

ISO 18187:2016 specifies a rapid method for assessing solid samples in an aerobic suspension, by determining the inhibition of dehydrogenase activity of Arthrobacter globiformis using the redox dye resazurin.
It is applicable for assessing the effect of water-soluble and solid matter bounded non-volatile contaminants of natural samples, such as soils and waste materials. The test yields a result within 6 h and can therefore be used for screening potentially contaminated material.

Bodenbeschaffenheit - Feststoffkontakttest unter Verwendung der Dehydrogenaseaktivität von Arthrobacter globiformis (ISO 18187:2024)

Dieses Dokument legt ein Schnellverfahren zur Beurteilung von Feststoffproben in einer aeroben Suspension durch Bestimmung der Hemmung der Dehydrogenaseaktivität von Arthrobacter globiformis unter Verwendung des Redox-Farbstoffes Resazurin fest.
Sie gilt für die Beurteilung der Auswirkung von wasserlöslichen und feststoffgebundenen nicht flüchtigen Verunreinigungen in natürlichen Proben, wie Böden und Abfällen. Obwohl dies nicht der Hauptzweck ist, kann der Kontakttest auch zur Prüfung der Wirkung von Chemikalien verwendet werden, wie in Anhang C beschrieben. Die Prüfung führt innerhalb von 6 h zu einem Ergebnis und kann deshalb zum Screening potentiell verunreinigten Test-Materials eingesetzt werden.

Qualité du sol - Essai contact pour échantillons solides utilisant l'activité déshydrogénase de Arthrobacter globiformis (ISO 18187:2024)

ISO 18187:2016 spécifie une méthode rapide d'évaluation d'échantillons solides en suspension aérobie, en déterminant l'inhibition de l'activité déshydrogénase de Arthrobacter globiformis à l'aide d'un indicateur redox coloré, la résazurine.
Cette méthode est applicable à l'évaluation de l'effet de contaminants non volatils, liés aux matières solides ou solubles dans l'eau, présents dans des échantillons naturels tels que des sols et des déchets. L'essai délivre un résultat en 6 h et peut donc être utilisé pour le criblage de matériaux potentiellement contaminés.

Kakovost tal - Kontaktni preskus za trdne vzorce z dehidrogenazno aktivnostjo Arthrobacter globiformis (ISO 18187:2024)

General Information

Status
Published
Public Enquiry End Date
19-Sep-2023
Publication Date
12-Nov-2024
ICS
13.080.30 - Biological properties of soils
Technical Committee
KAT - Soil quality
Current Stage
6060 - National Implementation/Publication (Adopted Project)
Start Date
27-Sep-2024
Due Date
02-Dec-2024
Completion Date
13-Nov-2024
Ref Project
EN ISO 18187:2024 - Soil quality - Contact test for solid samples using the dehydrogenase activity of Arthrobacter globiformis (ISO 18187:2024)

Relations

Replaces
SIST EN ISO 18187:2018 - Soil quality - Contact test for solid samples using the dehydrogenase activity of Arthrobacter globiformis (ISO 18187:2016)
Effective Date
01-Dec-2024

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SLOVENSKI STANDARD
01-december-2024
Nadomešča:
SIST EN ISO 18187:2018
Kakovost tal - Kontaktni preskus za trdne vzorce z dehidrogenazno aktivnostjo
Arthrobacter globiformis (ISO 18187:2024)
Soil quality - Contact test for solid samples using the dehydrogenase activity of
Arthrobacter globiformis (ISO 18187:2024)
Bodenbeschaffenheit - Feststoffkontakttest unter Verwendung der
Dehydrogenaseaktivität von Arthrobacter globiformis (ISO 18187:2024)
Qualité du sol - Essai contact pour échantillons solides utilisant l'activité déshydrogénase
de Arthrobacter globiformis (ISO 18187:2024)
Ta slovenski standard je istoveten z: EN ISO 18187:2024
ICS:
13.080.30 Biološke lastnosti tal Biological properties of soils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 18187
EUROPEAN STANDARD
NORME EUROPÉENNE
May 2024
EUROPÄISCHE NORM
ICS 13.080.30 Supersedes EN ISO 18187:2018
English Version
Soil quality - Contact test for solid samples using the
dehydrogenase activity of Arthrobacter globiformis (ISO
18187:2024)
Qualité du sol - Essai contact pour échantillons solides Bodenbeschaffenheit - Feststoffkontakttest unter
utilisant l'activité déshydrogénase de Arthrobacter Verwendung der Dehydrogenaseaktivität von
globiformis (ISO 18187:2024) Arthrobacter globiformis (ISO 18187:2024)
This European Standard was approved by CEN on 15 May 2024.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2024 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 18187:2024 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 18187:2024) has been prepared by Technical Committee ISO/TC 190 "Soil
quality" in collaboration with Technical Committee CEN/TC 444 “Environmental characterization of
solid matrices” the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by November 2024, and conflicting national standards
shall be withdrawn at the latest by November 2024.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 18187:2018.
Any feedback and questions on this document should be directed to the users’ national standards
body/national committee. A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the
United Kingdom.
Endorsement notice
The text of ISO 18187:2024 has been approved by CEN as EN ISO 18187:2024 without any modification.

International
Standard
ISO 18187
Second edition
Soil quality — Contact test for solid
2024-05
samples using the dehydrogenase
activity of Arthrobacter globiformis
Qualité du sol — Essai contact pour échantillons solides utilisant
l'activité déshydrogénase de Arthrobacter globiformis
Reference number
ISO 18187:2024(en) © ISO 2024
ISO 18187:2024(en)
© ISO 2024
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii
ISO 18187:2024(en)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 4
5 Reagents and material . 4
5.1 Test organisms .4
5.2 Control substrates .4
5.2.1 General .4
5.2.2 Control for soils .4
5.2.3 Control for waste material .5
5.3 Test substrates . . .5
5.4 Reagents .6
6 Apparatus . 8
7 Procedure . 9
7.1 Preparation of dilutions .9
7.2 Reference substances and positive control preparation .9
7.3 Contact test procedure .10
7.3.1 General .10
7.3.2 Aeration .10
7.3.3 Deactivation .11
7.3.4 Preparation of the inoculum .11
7.3.5 Incubation and fluorescence measurement .11
7.4 Interferences .11
8 Calculation and expression of results .12
8.1 Calculation . 12
8.1.1 Relative fluorescence . 12
8.1.2 Determining the percentage of inhibition . 12
8.2 Expression of results . . 12
9 Validity of the test .13
10 Statistical analysis .13
11 Test report . 14
Annex A (informative) Results on the interlaboratory test .15
Annex B (informative) Preparation of test organisms .22
Annex C (informative) Testing chemical substances .24
Bibliography .26

iii
ISO 18187:2024(en)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out through
ISO technical committees. Each member body interested in a subject for which a technical committee
has been established has the right to be represented on that committee. International organizations,
governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely
with the International Electrotechnical C
...

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