SIST EN ISO 12872:2023

Olive oils and olive-pomace oils - Determination of the 2-glyceryl monopalmitate content (ISO 12872:2022)

This document specifies a procedure for the determination of the content, as a percentage mass fraction, of 2-glyceryl monopalmitate in olive oils and olive-pomace oils that are liquid at ambient temperature (20 °C).
NOTE       This document is based on COI/T.20/Doc. No 23/Rev.1:2017[7].

Olivenöle und Oliventresteröle - Bestimmung des Gehalts an 2‑Glycerylmonopalmitat (ISO 12872:2022)

Dieses Dokument legt ein Verfahren fest zur Bestimmung des Gehalts, als Massenanteil in Prozent, an 2 Glycerinmonopalmitat in Olivenölen und Oliventresterölen, die bei Umgebungstemperatur (20 °C) flüssig sind.
ANMERKUNG   Dieses Dokument basiert auf COI/T.20/Doc. No 23/Rev.1:2017 [7].

Huiles d'olive et huiles de grignons d'olive - Détermination de la teneur en 2-glycéryl monopalmitate (ISO 12872:2022)

Le présent document spécifie une méthode pour la détermination de la teneur, sous forme de fraction massique en pourcentage, en 2-glycéryl monopalmitate des huiles d’olive et des huiles de grignons d’olive liquides à température ambiante (20 °C).
NOTE      Le présent document est fondé sur la COI/T.20/Doc. 23/Rév. 1:2017[7].

Oljčna olja in olja iz oljčnih tropin - Določevanje vsebnosti 2-gliceril monopalmitata (ISO 12872:2022)

Standard ISO 12872:2010 navaja postopek za določevanje vsebnosti 2-gliceril monopalmitata kot odstotek masnega deleža v oljčnih oljih in oljih iz oljčnih tropin, ki so pri temperaturi okolja (20° C) v tekočem stanju.

General Information

• Status: Published
• Public Enquiry End Date: 30-May-2022
• Publication Date: 07-Dec-2022
• ICS: 67.200.10 - Animal and vegetable fats and oils
• Technical Committee: KŽP - Agricultural food products
• Current Stage: 6060 - National Implementation/Publication (Adopted Project)
• Start Date: 01-Dec-2022
• Due Date: 05-Feb-2023
• Completion Date: 08-Dec-2022
• Ref Project: EN ISO 12872:2022 - Olive oils and olive-pomace oils - Determination of the 2-glyceryl monopalmitate content (ISO 12872:2022)

Relations

• Replaces: SIST EN ISO 12872:2014 - Olive oils and olive-pomace oils - Determination of the 2-glyceryl monopalmitate content (ISO 12872:2010)
• Effective Date: 01-Jan-2023

Standards Content (Sample)

SLOVENSKI STANDARD
01-januar-2023
Nadomešča:
SIST EN ISO 12872:2014
Oljčna olja in olja iz oljčnih tropin - Določevanje vsebnosti 2-gliceril monopalmitata
(ISO 12872:2022)
Olive oils and olive-pomace oils - Determination of the 2-glyceryl monopalmitate content
(ISO 12872:2022)
Olivenöle und Oliventresteröle - Bestimmung des Gehalts an 2‑Glycerylmonopalmitat
(ISO 12872:2022)
Huiles d'olive et huiles de grignons d'olive - Détermination de la teneur en 2-glycéryl
monopalmitate (ISO 12872:2022)
Ta slovenski standard je istoveten z: EN ISO 12872:2022
ICS:
67.200.10 Rastlinske in živalske Animal and vegetable fats
maščobe in olja and oils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 12872
EUROPEAN STANDARD
NORME EUROPÉENNE
September 2022
EUROPÄISCHE NORM
ICS 67.200.10 Supersedes EN ISO 12872:2014
English Version
Olive oils and olive-pomace oils - Determination of the 2-
glyceryl monopalmitate content (ISO 12872:2022)
Huiles d'olive et huiles de grignons d'olive - Olivenöle und Oliventresteröle - Bestimmung des
Détermination de la teneur en 2-glycéryl Gehalts an 2-Glycerylmonopalmitat (ISO 12872:2022)
monopalmitate (ISO 12872:2022)
This European Standard was approved by CEN on 20 September 2022.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2022 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 12872:2022 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 12872:2022) has been prepared by Technical Committee ISO/TC 34 "Food
products" in collaboration with Technical Committee CEN/TC 307 “Oilseeds, vegetable and animal fats
and oils and their by-products - Methods of sampling and analysis” the secretariat of which is held by
AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by March 2023, and conflicting national standards shall
be withdrawn at the latest by March 2023.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 12872:2014.
Any feedback and questions on this document should be directed to the users’ national standards
body/national committee. A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the
United Kingdom.
Endorsement notice
The text of ISO 12872:2022 has been approved by CEN as EN ISO 12872:2022 without any modification.

INTERNATIONAL ISO
STANDARD 12872
Second edition
2022-08
Olive oils and olive-pomace oils —
Determination of the 2-glyceryl
monopalmitate content
Huiles d'olive et huiles de grignons d'olive — Détermination de la
teneur en 2-glycéryl monopalmitate
Reference number
ISO 12872:2022(E)
ISO 12872:2022(E)
© ISO 2022
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: [phone]
Email: [e-mail]
Website: www.iso.org
Published in Switzerland
ii
ISO 12872:2022(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagents . 1
6 Apparatus . 3
7 Sampling . 4
8 Preparation of the test sample. 4
9 Procedure .4
9.1 Preparatory steps . 4
9.2 Column chromatography . 5
9.2.1 Conventional procedure . 5
9.2.2 Procedure when ready-to-use silica SPE cartridges are employed . 5
9.3 Hydrolysis with pancreatic lipase . 5
9.4 Preparation of the silylated derivatives and gas chromatography . 5
9.5 Gas chromatography . 5
9.5.1 Operating conditions . 5
9.5.2 Identification of peaks . 6
9.5.3 Quantitative evaluation . 6
10 Expression of results . 6
11 Precision . 6
11.1 Interlaboratory test . . 6
11.2 Repeatability . 6
11.3 Reproducibility . 6
12 Test report . 7
Annex A (informative) Chromatograms . 8
Annex B (informative) Results of interlaboratory test and comparison study .11
Annex C (informative) Preparation and activity of the lipase .13
Bibliography .15
iii
ISO 12872:2022(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
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ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www.iso.org/patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO’s adherence to
the World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT), see
www.iso.org/iso/foreword.html.
This document was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 11,
Animal and vegetable fats and oils, in collaboration with the European Committee for Standardization
(CEN) Technical Committee CEN/TC 307, Oilseeds, vegetable and animal fats and oils and their by-products
— Methods of sampling and analysis, in accordance with the Agreement on technical cooperation
between ISO and CEN (Vienna Agreement).
This sec
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