SIST EN 17720:2025
(Main)Plant biostimulants - Determination of enterococci
Plant biostimulants - Determination of enterococci
This European Standard establishes the methodology for the determination of Enterococcaceae in plant biostimulant products in accordance to the future Regulation of EU fertilising products.
Pflanzen-Biostimulanzien - Bestimmung von enterococci
Dieses Verfahren wurde zur Bestimmung von Enterokokken in Biostimulanzien als einzelner Mikroorganismus-Bestandteil oder in einem Gemisch mit anderen Mikroorganismen entwickelt. Dieses Dokument ist nicht anwendbar auf mineralische Düngemittel, die als Ergänzungsfuttermittel festgelegt sind, hauptsächlich aus Mineralstoffen zusammengesetzt sind und mindestens 40 % Rohasche enthalten (Richtlinie 79/373/EWG) [3].
Biostimulants des végétaux - Détermination des enterococci
Cette méthodologie a été développée pour déterminer les entérocoques dans les biostimulants en tant que composant unique d’un microorganisme ou dans un mélange avec d’autres microorganismes. Le présent document ne s’applique pas aux engrais minéraux utilisés comme aliments complémentaires des animaux, principalement composés de minéraux et contenant au moins 40 % de cendres brutes (Directive du Conseil 79/373/CEE) [3].
Rastlinski biostimulanti - Določanje enterokokov
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-februar-2025
Nadomešča:
SIST-TS CEN/TS 17720:2023
Rastlinski biostimulanti - Določanje enterokokov
Plant biostimulants - Determination of enterococci
Pflanzen-Biostimulanzien - Bestimmung von enterococci
Biostimulants des végétaux - Détermination des enterococci
Ta slovenski standard je istoveten z: EN 17720:2024
ICS:
65.080 Gnojila Fertilizers
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN 17720
EUROPEAN STANDARD
NORME EUROPÉENNE
November 2024
EUROPÄISCHE NORM
ICS 65.080 Supersedes CEN/TS 17720:2022
English Version
Plant biostimulants - Determination of enterococci
Biostimulants des végétaux - Détermination des Pflanzen-Biostimulanzien - Bestimmung von
enterococci enterococci
This European Standard was approved by CEN on 26 August 2024.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2024 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 17720:2024 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references. 5
3 Terms and definitions . 6
4 Principle . 6
5 Diluents, selective media and test kit for phenotypic characterization . 6
5.1 General . 6
5.2 Selective media . 7
5.3 Phenotypic characterization and confirmation . 7
6 Apparatus and glassware . 7
7 Sampling . 7
8 Preparation of test sample . 8
9 Procedure . 8
9.1 Inoculation and incubation on Slanetz-Bartley agar. . 8
9.2 Evaluation of results. 8
9.3 Confirmation step on BEA . 8
9.4 Catalase test (optional) . 9
10 Expression of results . 9
11 Precision . 9
11.1 General . 9
11.2 Interlaboratory study . 9
11.3 Repeatability . 9
11.4 Reproducibility . 9
12 Test report . 10
Annex A (normative) Composition and preparation of culture media and reagents . 11
Annex B (informative) Repeatability and reproducibility of the method . 14
Annex ZA (informative) Relationship of this European Standard and the essential requirements
of Regulation (EU) 2019/1009 making available on the market of EU fertilising products aimed to
be covered . 17
Bibliography . 18
European foreword
This document (EN 17720:2024) has been prepared by Technical Committee CEN/TC 455 “Plant
Biostimulants”, the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by May 2025, and conflicting national standards shall be
withdrawn at the latest by May 2025.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes CEN/TS 17720:2022.
— the title;
— the European foreword and Introduction have been updated for consistency with other standards;
— the Scope has been reworded for clarification;
— the normative references have been updated;
— in Clause 3 the definitions of presumptive enterococci and enterococci have been revised;
— in Clause 7 and Clause 8 the sampling and sample preparation instructions have been replaced by
the relevant normative references;
— in Clause 9, the procedure has been clarified and references to the media and apparatus required
have been added.
— Annex A and Annex B have been revised for consistency with other standards.
— Annex ZA has been added in line with the clarified Scope of the method.
This document has been prepared under a standardization request addressed to CEN by the European
Commission. The Standing Committee of the EFTA States subsequently approves these requests for its
Member States.
For relationship with EU Directive(s) / Regulation(s), see informative Annex ZA, which is an integral part
of this document.
Any feedback and questions on this document should be directed to the users’ national standards body.
A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organisations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia,
Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland,
Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of North
Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the United
Kingdom.
Introduction
The European Committee for Standardization (CEN) was requested by the European Commission (EC) to
draft European Standards or European Standardization deliverables to support the implementation of
Regulation (EU) 2019/1009 of 5 June 2019 [1] laying down rules on the making available on the market
of EU fertilising products (“FPR” or “Fertilising Products Regulation”).
This standardization request, presented as SR M/564 and relevant amendments, also contributes to the
Communication on “Innovating for Sustainable Growth: A Bio economy for Europe”. The interest in plant
biostimulants has increased significantly in Europe as a valuable tool to use in agriculture.
Standardization was identified as having an important role in order to promote the use of biostimulants.
The work of CEN/TC 455 seeks to improve the reliability of the supply chain, thereby improving the
confidence of farmers, industry, and consumers in biostimulants, and will promote and support
commercialisation of the European biostimulant industry.
WARNING — Persons using this document should be familiar with normal laboratory practice. This
document does not purport to address all of the safety problems, if any, associated with its use. It is the
responsibility of the user to establish appropriate safety and health practices and to ensure compliance
with any national regulatory conditions.
IMPORTANT — It is absolutely essential that tests conducted in accordance with this document be
carried out by suitably trained staff
1 Scope
This document specifies a method for the enumeration of enterococci in microbial and non-microbial
plant biostimulants. This document specifies a colony-count technique on a selective medium (Slanetz-
Bartley agar) with confirmation on Bile Esculin Azide agar.
This document is applicable to all formulations of microbial and non-microbial plant biostimulants in
liquid or solid form. This document is not applicable to other fertilizing products.
This document is applicable to the blends of fertilizing products where a blend is a mix of at least two of
the following component EU fertilising products categories: Fertilizers, Liming Materials, Soil Improvers,
Growing Media, Plant Biostimulants, and where the following category Plant Biostimulants is the highest
% in the blend by mass or volume, or in the case of liquid form by dry mass. If Plant Biostimulants is not
the highest % in the blend, the European Standard for the highest % of the blend applies. In case a blend
of fertilizing products is composed of components in equal quantity or in case the component EU
fertilising products used for the blend have identical formulations , the user decides which standard to
apply.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
EN ISO 7218:2024, Microbiology of food and animal feeding stuffs — General requirements and guidance
for microbiological examinations
EN 17702-1:2024, Plant biostimulants — Sampling and sample preparation — Part 1: Sampling
EN 17708:2024, Plant biostimulants — Preparation of sample for microbial analysis
EN 17714:2024, Plant biostimulants — Determination of microorganisms concentration
EN 17724:2024, Plant biostimulants — Terminology
An example of such a blend is a product with 2 claimed functions consisting of a non-microbial plant biostimulant and an
organic fertilizer composed of 1kg/kg of plant biostimulant from seaweed.
3 Terms and definitions
For the purposes of this document, the terms and definitions given in EN 17724:2024 and the following
apply.
ISO and IEC maintain terminology databases for use in standardization at the following addresses:
• ISO Online browsing platform: available at https://www.iso.org/obp
• IEC Electropedia: available at https://www.electropedia.org/
3.1
presumptive enterococci
Gram-positive, catalase-negative cocci, able to reduce 2,3,5-triphenyl tetrazolium chloride to formazan
on Slanetz-Bartley agar under the conditions specified in this document
3.2
enterococci
bacteria which are able to reduce 2,3,5-triphenyltetrazolium chloride to formazan on the surface of a
selective culture medium containing sodium azide (Slanetz-Bartley agar) and to hydrolyse esculin at
44 °C on a medium containing bile salts (Bile Esculin Azide agar)
...
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