SIST EN ISO 8292-1:2010

Animal and vegetable fats and oils - Determination of solid fat content by pulsed NMR - Part 1: Direct method (ISO 8292-1:2008)

This part of ISO 8292 specifies a direct method for the determination of solid fat content in animal and vegetable fats and oils (hereafter designated “fats”) using low-resolution pulsed nuclear magnetic resonance (NMR) spectrometry. Two alternative thermal pre-treatments are specified: one for general purpose fats not exhibiting pronounced polymorphism and which stabilize mainly in the  polymorph; and one for fats similar to cocoa butter which exhibit pronounced polymorphism and stabilize in the  polymorph. Additional thermal pre-treatments, which may be more suitable for specific purposes, are given in an informative annex. The direct method is easy to carry out and is reproducible, but is not as accurate as the indirect method due to the approximate method of calculation. NOTE An indirect method is specified in ISO 8292-2.

Tierische und pflanzliche Fette und Öle - Bestimmung des Festanteils von Fett durch das Verfahren mit gepulster magnetischer Kernresonanz - Teil 1: Direktes Verfahren (ISO 8292-1:2008)

Dieser Teil von ISO 8292 legt ein indirektes Verfahren zur Bestimmung des Festanteils von tierischen und pflanzlichen Fetten und Ölen (nachfolgend als „Fette“ bezeichnet) mittels gepulster magnetischer Kern-resonanz (en: nuclear magnetic resonance, NMR) mit niedriger Auflösung fest.
Zwei alternative thermische Vorbehandlungen sind festgelegt: eine für Fette für die allgemeine Verwendung, die keine ausgeprägte Polymorphie aufweisen und die sich in der  Polymorphie verfestigen; und eine für kakaobutterähnliche Fette, die eine ausgeprägte Polymorphie aufweisen und die sich in der  Polymorphie verfestigen. Zusätzliche thermische Vorbehandlungen, die für bestimmte Zwecke geeigneter sein können, sind in einem informativen Anhang angegeben.
Das indirekte Verfahren ist nicht so einfach durchzuführen und weniger reproduzierbar als das direkte Verfahren, aber es ist genauer und universeller anwendbar auf alle Fette.
ANMERKUNG   Ein direktes Verfahren ist in ISO 8292 1 festgelegt.

Corps gras d'origines animale et végétale - Détermination de la teneur en corps gras solides par RMN pulsée - Partie 1: Méthode directe (ISO 8292-1:2008)

L'ISO 8292-1:2008 spécifie une méthode directe pour la détermination de la teneur en corps gras solides dans des corps gras d'origine animale et végétale (appelés ci-après «corps gras») en utilisant un spectromètre de résonance magnétique nucléaire (RMN) pulsée à basse résolution.
Deux prétraitements thermiques possibles sont spécifiés: l'un pour les corps gras à usage général ne présentant pas de polymorphisme prononcé et qui se stabilisent principalement sous la forme polymorphe β', et l'autre pour des corps gras similaires au beurre de cacao qui présentent un polymorphisme prononcé et qui se stabilisent sous la forme polymorphe β. Des prétraitements thermiques supplémentaires, qui peuvent être plus adaptés pour des applications spécifiques, sont indiqués dans une annexe informative.
La méthode directe est facile à conduire et est reproductible, mais n'est pas aussi précise que la méthode indirecte en raison de la méthode de calcul approximative.

Živalske in rastlinske maščobe in olja - Določevanje trdnih maščob s pulzno jedrsko magnetno resonanco - 1. del: Neposredna metoda (ISO 8292-1:2008)

Ta del ISO 8292 določa direktno metodo za določevanje trdnih maščob v živalskih in rastlinskih maščobah in oljih (v nadaljevanju navedeno kot »maščobe«) z nizko resolucijsko pulzno jedrsko magnetsko resonančno (NMR) spektrometrijo. Določeni sta dve alternativni toplotni predobdelavi:  Ena za maščobe za splošno uporabo, ki ne izkazujejo poudarjenega polimorfizma in se stabilizirajo predvsem v  polimorfih; in ena za maščobe, ki so podobne kokosovemu maslu in ki izkazujejo poudarjen polimorfizem ter se stabilizirajo v  polimorfih. Dodatne toplotne predobdelave, ki so lahko bolj primerne za določene namene, so podane v informativnem dodatku.  Neposredna metoda se zlahka izvede in je ponovljiva, vendar ni tako natančna kot posredna metoda zaradi približnih metod izračuna. OPOMBA: Posredna metoda je določena v ISO 8292-2.

General Information

• Status: Published
• Publication Date: 18-Jul-2010
• ICS: 67.200.10 - Animal and vegetable fats and oils
• Technical Committee: KŽP - Agricultural food products
• Current Stage: 6060 - National Implementation/Publication (Adopted Project)
• Start Date: 08-Jun-2010
• Due Date: 13-Aug-2010
• Completion Date: 19-Jul-2010
• Ref Project: EN ISO 8292-1:2010 - Animal and vegetable fats and oils - Determination of solid fat content by pulsed NMR - Part 1: Direct method (ISO 8292-1:2008)

Relations

• Replaces: SIST EN ISO 8292:1998 - Animal and vegetable fats and oils - Determination of solid fat content - Pulsed nuclear magnetic resonance method (ISO 8292:1991)
• Effective Date: 01-Sep-2010
• Replaces: SIST EN ISO 8292:1998 - Animal and vegetable fats and oils - Determination of solid fat content - Pulsed nuclear magnetic resonance method (ISO 8292:1991)
• Effective Date: 08-Jun-2022

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN ISO 8292-1:2010
01-september-2010
1DGRPHãþD
SIST EN ISO 8292:1998
äLYDOVNHLQUDVWOLQVNHPDãþREHLQROMD'RORþHYDQMHWUGQLKPDãþREVSXO]QR
MHGUVNRPDJQHWQRUHVRQDQFRGHO1HSRVUHGQDPHWRGD,62
Animal and vegetable fats and oils - Determination of solid fat content by pulsed NMR -
Part 1: Direct method (ISO 8292-1:2008)
Tierische und pflanzliche Fette und Öle - Bestimmung des Festanteils von Fett durch das
Verfahren mit gepulster magnetischer Kernresonanz - Teil 1: Direktes Verfahren (ISO
8292-1:2008)
Corps gras d'origines animale et végétale - Détermination de la teneur en corps gras
solides par RMN pulsée - Partie 1: Méthode directe (ISO 8292-1:2008)
Ta slovenski standard je istoveten z: EN ISO 8292-1:2010
ICS:
67.200.10 5DVWOLQVNHLQåLYDOVNH Animal and vegetable fats
PDãþREHLQROMD and oils
SIST EN ISO 8292-1:2010 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN ISO 8292-1:2010

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SIST EN ISO 8292-1:2010


EUROPEAN STANDARD
EN ISO 8292-1

NORME EUROPÉENNE

EUROPÄISCHE NORM
April 2010
ICS 67.200.10 Supersedes EN ISO 8292:1995
English Version
Animal and vegetable fats and oils - Determination of solid fat
content by pulsed NMR - Part 1: Direct method (ISO 8292-
1:2008)
Corps gras d'origines animale et végétale - Détermination Tierische und pflanzliche Fette und Öle - Bestimmung des
de la teneur en corps gras solides par RMN pulsée - Partie Festanteils von Fett durch das Verfahren mit gepulster
1: Méthode directe (ISO 8292-1:2008) magnetischer Kernresonanz - Teil 1: Direktes Verfahren
(ISO 8292-1:2008)
This European Standard was approved by CEN on 18 March 2010.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN Management Centre or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as the
official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland,
Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.






EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2010 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 8292-1:2010: E
worldwide for CEN national Members.

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SIST EN ISO 8292-1:2010
EN ISO 8292-1:2010 (E)
Contents Page
Foreword .3

2

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SIST EN ISO 8292-1:2010
EN ISO 8292-1:2010 (E)
Foreword
The text of ISO 8292-1:2008 has been prepared by Technical Committee ISO/TC 34 “Food products” of the
International Organization for Standardization (ISO) and has been taken over as EN ISO 8292-1:2010 by
Technical Committee CEN/TC 307 “Oilseeds, vegetable and animal fats and oils and their by-products -
Methods of sampling and analysis” the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by October 2010, and conflicting national standards shall be withdrawn at
the latest by October 2010.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 8292:1995.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech
Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia,
Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain,
Sweden, Switzerland and the United Kingdom.
Endorsement notice
The text of ISO 8292-1:2008 has been approved by CEN as a EN ISO 8292-1:2010 without any modification.

3

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SIST EN ISO 8292-1:2010

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SIST EN ISO 8292-1:2010

INTERNATIONAL ISO
STANDARD 8292-1
First edition
2008-04-01

Animal and vegetable fats and oils —
Determination of solid fat content by
pulsed NMR —
Part 1:
Direct method
Corps gras d'origines animale et végétale — Détermination de la teneur
en corps gras solides par RMN pulsée —
Partie 1: Méthode directe




Reference number
ISO 8292-1:2008(E)
©
ISO 2008

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SIST EN ISO 8292-1:2010
ISO 8292-1:2008(E)
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©  ISO 2008
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means,
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ii © ISO 2008 – All rights reserved

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SIST EN ISO 8292-1:2010
ISO 8292-1:2008(E)
Contents Page
Foreword. iv
1 Scope.1
2 Normative references.1
3 Terms and definitions .1
4 Symbols and abbreviated terms .2
5 Principle.3
6 Apparatus.3
7 Sampling.5
8 Procedure.5
8.1 Measurement protocol and test sample.5
8.2 Oven, water baths and temperature-controlled blocks .7
8.3 Determination of the conversion factor (where necessary).7
8.4 NMR spectrometer.8
8.5 Filling the measurement tubes.8
8.6 Removing the thermal history.8
8.7 Equilibrating at the initial temperature.8
8.8 Crystallization and tempering .9
8.9 Measuring the SFC .9
8.10 Number of determinations .10
8.11 Cleaning the measurement tubes .10
9 Expression of results.10
10 Precision.11
10.1 Interlaboratory test .11
10.2 Repeatability.11
10.3 Reproducibility.11
11 Test report.12
Annex A (informative) Results of interlaboratory tests.13
Annex B (informative) Theory of the direct method .23
Annex C (informative) Additional measurement protocols.25
Bibliography .27

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SIST EN ISO 8292-1:2010
ISO 8292-1:2008(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 8292-1 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 11, Animal
and vegetable fats and oils.
This part of ISO 8292, together with ISO 8292-2, cancel and replace ISO 8292:1991.
ISO 8292 consists of the following parts, under the general title Animal and vegetable fats and oils —
Determination of solid fat content by pulsed NMR:
⎯ Part 1: Direct method
⎯ Part 2: Indirect method

iv © ISO 2008 – All rights reserved

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SIST EN ISO 8292-1:2010
INTERNATIONAL STANDARD ISO 8292-1:2008(E)

Animal and vegetable fats and oils — Determination of solid fat
content by pulsed NMR —
Part 1:
Direct method
1 Scope
This part of ISO 8292 specifies a direct method for the determination of solid fat content in animal and
vegetable fats and oils (hereafter designated “fats”) using low-resolution pulsed nuclear magnetic resonance
(NMR) spectrometry.
Two alternative thermal pre-treatments are specified: one for general purpose fats not exhibiting pronounced
polymorphism and which stabilize mainly in the β′-polymorph; and one for fats similar to cocoa butter which
exhibit pronounced polymorphism and stabilize in the β-polymorph. Additional thermal pre-treatments, which
may be more suitable for specific purposes, are given in an informative annex.
The direct method is easy to carry out and is reproducible, but is not as accurate as the indirect method due to
the approximate method of calculation.
NOTE An indirect method is specified in ISO 8292-2.
2 Normative references
The following referenced documents are indispensable for the application of this document. For dated
references, only the edition cited applies. For undated references, the latest edition of the referenced
document (including any amendments) applies.
ISO 661, Animal and vegetable fats and oils — Preparation of test sample
ISO 8292-2, Animal and vegetable fats and oils — Determination of solid fat content by pulsed NMR — Part 2:
Indirect method
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
solid fat content
SFC
ratio as a percentage of the number of protons in the solid phase to the number of protons in the solid and
liquid phase at a specified temperature
NOTE SFC expressed on this basis is taken to be numerically equivalent to the percentage mass fraction of fat in the
solid state. No correction is made for the different densities of protons in the solid and liquid phases, because this would
require exact knowledge of the composition of the solid and liquid phases of the fat blends at each temperature.
Regardless of any other systematic errors, this means that SFC values obtained by this method are about 0,5 % to 1,0 %
higher than the true solid fat percentage mass fraction.
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SIST EN ISO 8292-1:2010
ISO 8292-1:2008(E)
3.2
liquid fat content
percentage mass fraction of fat in the liquid state at a specified temperature
NOTE The liquid fat content is equal to 100 − w , where w is the solid fat content.
SFC SFC
3.3
tempering
thermal treatment of the fat, after crystallization and prior to equilibration at the measurement temperature,
which consists of holding the fat at a specified temperature for a specified time to transform the fat to a
desired polymorph, and/or to ensure that a desired phase equilibrium has been achieved and/or to ensure that
crystallization is complete
3.4
measurement temperature
temperature at which the solid fat content is determined
3.5
repetition time
interval between successive pulses
3.6
dead time
time during which the instrument receiver is unable to record the decay signal
NOTE Dead time is usually less than 10 µs after the pulse.
3.7
measurement protocol
complete description of the solid fat content determination specifying application, instrumental conditions,
method, tempering, and whether measurements are in series or in parallel
NOTE Measurement protocols are listed in Table 1 and Annex C.
4 Symbols and abbreviated terms
f conversion (extrapolation) factor to correct the NMR signal observed at 11 µs to that at time zero
n number of pulses
p
S magnetization decay signal measured at about 11 µs
1
S magnetization decay signal measured at about 70 µs
2
SFC solid fat content
S magnetization decay signal corresponding to the liquid phase
L
S magnetization decay signal corresponding to the solid phase
S
S magnetization decay signals corresponding to both solid plus liquid phases
S+L
t repetition time
rep
w “true” SFC (measured in accordance with ISO 8292-2)
SFC,i
w SFC at measurement temperature, T
SFC,T
2 © ISO 2008 – All rights reserved

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SIST EN ISO 8292-1:2010
ISO 8292-1:2008(E)
5 Principle
The sample is tempered to a stable state at a specific temperature and then heated to, and stabilized at, the
measurement temperature. Unless otherwise specified, measurement temperatures can be any or all of: 0 °C;
5 °C; 10 °C; 15 °C; 20 °C; 25 °C; 27,5 °C; 30 °C; 32,5 °C; 35 °C; 37,5 °C; 40 °C; 45 °C; 50 °C; 55 °C; 60 °C.
After electromagnetic equilibration in the static magnetic field of the NMR spectrometer and application of a
90º radio frequency pulse, the magnetization decay signals from the protons in the solid and liquid phases are
recorded at about 11 µs and about 70 µs (or at times recommended by the spectrometer manufacturer,
see 6.1). SFC is then calculated.
Measurements may be made in series or in parallel.
One tube is filled from each test sample when making measurements in series. After tempering as required
and holding at 0 °C, the measurement tube is moved to the first measurement temperature, held for the
specified time, the SFC measured, and then moved to the second measurement temperature, and so on.
Thus, only one tube is required for all test samples, regardless of how many measurement temperatures are
used. However, the SFC recorded at a given measurement temperature depends on the preceding
measurement temperatures and times.
As many measurement tubes are filled from each test sample as there are measurement temperatures when
making measurements in parallel. After tempering as required and holding at 0 °C, each measurement tube is
moved more or less simultaneously to each required measurement temperature and held for the specified
time before measuring the SFC.
Although more tubes are required for measurement in parallel than with that in series, each w
SFC,T
determination is independent of other determinations. Additionally, the total time for the measurements is
significantly shortened.
EXAMPLE For a holding time of 90 min at 0 °C and holding times of 60 min at measurement temperatures of 10 °C,
20 °C, 30 °C, and 40 °C, the series measurement would take 5,5 h, whereas the parallel measurement would take 2,5 h.
6 Apparatus
6.1 Pulsed nuclear magnetic resonance spectrometer, low resolution
The NMR spectrometer shall have:
a) a magnet with a sufficiently uniform field to ensure that the half-life of the magnetization of a reference
sample of liquid fat is longer than 1 000 µs;
b) a measurement dead time plus pulse width of less than 10 µs;
c) an automatic measuring device which operates as soon as the measurement tubes (6.2.1) are inserted;
d) an adjustable measurement repetition time;
e) a 10 mm measurement cell/probe for measurement tubes which is temperature controlled at 40 °C.
For exact magnetization decay signal times, refer to spectrometer manufacturer's instructions; these are
normally at about 11 µs and about 70 µs and should not need to be altered by the user.
For preference, the instrument should be equipped with a computer which automatically takes the required
measurements, performs the required calculations and presents the results directly on the computer screen or
other display.
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SIST EN ISO 8292-1:2010
ISO 8292-1:2008(E)
6.2 Tubes
6.2.1 Measurement tubes, of glass with plastic caps, with outer diameter (10 ± 0,25) mm, wall thickness
(0,9 ± 0,25) mm, and length at least 150 mm, or as specified by the NMR spectrometer manufacturer.
6.2.2 Calibration tubes, of known instrument response to calibrate the spectrometer and to check the
direct method.
NOTE Plastic-in-oil calibration materials with known responses, giving an f factor in the range 1,4 to 1,45 appropriate
for the instrument and for use with the non-stabilizing direct and other protocols (see Table 1 and Annex C) are supplied
by the instrument manufacturer in standard measurement tubes. Materials giving SFC mass fractions of 0 %, about 30 %
and about 70 % are suitable. These values are independent of temperature. The calibration tubes need re-calibration at
1)
intervals as specified by the supplier.
6.3 Temperature-maintenance equipment
6.3.1 General
In principle, temperature-controlled blocks (6.3.3) have advantages over water baths (6.3.2) because the
tubes can never come into contact with water. In practice, as with aluminium blocks in water baths, the tubes
can take a significant time to come to the set temperature. Heat transfer can be improved if the tube wells are
purged with a dry gas. Blocks are also more difficult to control precisely than water baths, although modern
electronic controls can provide the required precision.
6.3.2 Water baths
Baths are required at temperatures of (0 ± 0,1) °C, (60 ± 0,1) °C, and, to within ± 0,1 ºC, the measuring and
tempering temperatures required according to the measurement protocol chosen. For the 60 °C,
measurement temperature, and tempering temperature baths, temperature-controlled blocks (6.3.3) may be
substituted.
Each water bath shall be equipped with either one aluminium block (6.3.2.1) or one metal rack (6.3.2.2) to
accommodate measurement tubes (6.2.1) immersible in the water to a depth of 60 mm.
Metal racks are preferred to aluminium blocks, especially when a large number of test samples with high SFC
are being measured or when the rapid or ultra-rapid measurement protocols are being used. When using
aluminium blocks, there may be a significant time lag after the tube is inserted before the fat in the tube
reaches the set temperature of the water bath. The perceived advantage of blocks is that the tubes can
remain dry and do not need to be wiped dry with a paper tissue before insertion into the spectrometer. In
practice, however, it is usually found that due to splashing or condensation, the tubes do become wet so that
drying is always recommended, see 8.9.
6.3.2.1 Aluminium blocks, with holes of diameter (10,35 ± 0,1) mm, and depth 70 mm. The thickness of
the metal under the holes and the distance between the edge of a peripheral hole and the nearest side face
shall be 10 mm. The distance between the axes of two adjacent holes shall be at least 17 mm (centre to
centre).
6.3.2.2 Metal racks, open-sided, with holes of diameter 11 mm to 15 mm; the distance between the axes
of two adjacent holes shall be at least 20 mm (centre to centre).
6.3.3 Temperature-controlled blocks, with holes
The blocks, with electronic control, shall be capable of being maintained to within ± 0,1 ºC of a set
temperature. These blocks may be used instead of water baths [except the 0 °C bath (6.3.2), because of the
large amount of cooling required]. The diameter of the holes shall be (10,35 ± 0,1) mm.


1) It is expected that in the future “open and independent” standards will be available from the EU’s Institute for
Reference Materials and Measurements in Geel, Belgium. This information is given for the convenience of users of this
International Standard, and does not constitute an endorsement of these products by ISO.
4 © ISO 2008 – All rights reserved

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SIST EN ISO 8292-1:2010
ISO 8292-1:2008(E)
Blocks are particularly useful at temperatures of 35 °C or more when no cooling is required (assuming the
ambient room temperature is below 22 °C) and where temperature control is less critical because of the
usually lower absolute solid fat levels.
6.4 Oven, with fan
The oven shall be capable of being maintained at (80 ± 2) °C.
Since the purpose of the 80 °C temperature is to melt the test portion and destroy its previous thermal history,
it shall be at least 20 °C above the melting temperature of the fat. If this is not the case, then the oven
temperature shall be raised accordingly and the fact recorded in the test report (Clause 11). This is rarely
necessary, as the fats concerned contain large amounts of long-chain saturated fatty acids, e.g. fully
hydrogenated liquid vegetable oils.
Although a water bath (6.3.2) or temperature-controlled block (6.3.3) may be used for the 80 °C temperature,
it is preferable to use an oven. In a block or bath it is almost inevitable that fat will contact the sides, at a
temperature above that of immersion, when filling the tubes. An oven ensures that all the fat in the tube is
completely melted and there are no seed crystals remaining with an unknown thermal history which could
seed the molten fat when it is eventually moved to the 0 °C crystallization temperature. Thus, an oven is likely
to give more reliable and reproducible results.
6.5 Stop-clock
An analogue clock with a large sweep second hand is preferred, although a digital clock may be used.
7 Sampling
A representative sample shall have been sent to the laboratory. It shall not have been damaged or changed
during transport or storage.
Sampling is not part of the method specified in this part of ISO 8292. A recommended sampling method is
given in ISO 5555.
8 Procedure
8.1 Measurement protocol and test sample
Choose the required protocol from Table 1 according to the sample type and other requirements. For some
types or applications of fats, the protocols given in Table 1 are not appropriate. The measurement protocols
given in Annex C may be more suitable.
Prepare the test sample in accordance with ISO 661.
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SIST EN ISO 8292-1:2010
ISO 8292-1:2008(E)
6 © ISO 2008 – All rights reserved
Table 1 — Measurement protocols
Measurement Measurement
Second
Tempering
First time
protocol conditions
time at
Instrumental
at 0 °C
Applicable to
0 °C
conditions
Time Temp. Time
No. Name Type
min h ºC min min
Fats and blends (comprising mainly vegetable fats,
Non- f = 1,4 to 1,45; repetition
hydrogenated and/or interesterified) crystallizing in the β'-
a
time , t = 2 s;
1D stabilizing — — (60 ± 2) Parallel (30 ± 1)
rep
polymorph and as used for margarines, spreads, shortenings
b
direct No. pulses , n = 3
p
and other general food applications
Cocoa butter, cocoa butter equivalents and similar fats f = 1,6 to 1,65; repetition
β-Stabilizing
2D containing large amounts of 2-oleo-di-saturated time, t = 6 s; 26 Parallel
(90 ± 2) (40 ± 0,5) (90 ± 2) (60 ± 2)
rep
direct
c
triacylglycerols and crystallizing in the β-polymorph No. pulses , n = 1
p
a
Needs to be 6 s for fats in the β-polymorph.
b 2 2
Pulse data are averaged by the instrument. Ideally, three pulses are used, but some older instruments can be set to only either one or four (1 or 2 ) pulses, in which case use four pulses.
c
Use of three pulses would result in sufficient time in the measurement cell to cause the test portion to partially melt and the SFC to reduce during the measurement.

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SIST EN ISO 8292-1:2010
ISO 8292-1:2008(E)
8.2 Oven, water baths and temperature-controlled blocks
Set this equipment up for the required temperatures as specified in the protocol.
8.3 Determination of the conversion factor (where necessary)
Due to the dead time of the instrument, the first measurement can be made only after the signal from the solid
phase has reduced significantly. A conversion factor corrects approximately for this effect.
Although the calibration tubes containing plastic-in-oil standards give a reproducible, but only approximately
correct, conversion factor for the common β′-polymorphic general-purpose fats averaged over the temperature
range of interest, they do not give the correct factor for the β-polymorphic fats such as cocoa butter. For these
fats, and for any other fats or blends of fats for which the polymorphis
...