SIST EN 46-2:2016

Wood preservatives - Determination of the preventive action against recently hatched larvae of Hylotrupes bajulus (Linnaeus) - Part 2: Ovicidal effect (laboratory method)

This European Standard specifies a method for the determination of the preventive action of a wood preservative against eggs of Hylotrupes bajulus (Linnaeus) when the preservative is applied as a surface treatment to wood. This method is applicable to: - water-insoluble chemicals which are being studied as active insecticides; - organic formulations, as supplied or as prepared in the laboratory by dilution of concentrates; - organic water-dispersible formulations as supplied or as prepared in the laboratory by dilution of concentrates; or - water-soluble materials, for example salts. The method is applicable whether or not the test specimens have been subjected to appropriate ageing procedures.

Holzschutzmittel - Bestimmung der vorbeugenden Wirkung gegenüber frisch geschlüpften Larven von Hylotrupes bajulus (Linnaeus) - Teil 2: Ovizide Wirkung (Laboratoriumsverfahren)

Diese Europäische Norm legt ein Verfahren zur Bestimmung der vorbeugenden Wirkung eines Holzschutz-mittels gegenüber Eiern von Hylotrupes bajulus (Linnaeus) fest, wobei das Holzschutzmittel zur Oberflächen¬behandlung des Holzes verwendet wird.
Das Verfahren gilt für:
- wasserunlösliche Chemikalien, die als wirksame Insektizide untersucht werden;
- organische Formulierungen im Anlieferungszustand oder in Form von im Laboratorium hergestellten Verdünnungen von Konzentraten;
- organische, in Wasser dispergierbare Formulierungen im Anlieferungszustand oder in Form von im Laboratorium hergestellten Verdünnungen von Konzentraten; oder
- wasserlösliche Stoffe, zum Beispiel Salze.
Das Verfahren gilt unabhängig davon, ob die Prüfkörper einer angemessenen Alterungsbeanspruchung ausgesetzt wurden oder nicht.

Produits de préservation du bois - Détermination de l'action préventive contre les larves récemment écloses d'Hylotrupes bajulus (Linnaeus) - Partie 2 : Effet ovicide (Méthode de laboratoire)

La présente Norme européenne prescrit une méthode de détermination de l’action préventive d'un produit de préservation du bois contre les oeufs d’Hylotrupes bajulus (Linnaeus) lorsque le produit de préservation est appliqué au bois par un traitement de surface.
La présente méthode est applicable :
-   aux produits chimiques non hydrosolubles étudiés en tant que matières actives insecticides ;
-   aux formules organiques telles qu'elles sont fournies ou préparées en laboratoire par dilution de concentrés ;
-   aux formules organiques hydrodispersables telles qu'elles sont fournies ou préparées en laboratoire par dilution de concentrés ; ou
-   aux produits solubles dans l’eau, par exemple aux sels.
Cette méthode s’applique à des éprouvettes ayant subi ou non des épreuves de vieillissement appropriées.

Biocidni proizvodi za zaščito lesa - Ugotavljanje preventivnega delovanja proti hišnemu kozličku Hylotrupes bajulus (Linnaeus) - 2. del: Ugotavljanje učinkovitosti proti jajčecem (laboratorijska metoda)

Ta evropski standard določa metodo za ugotavljanje preventivnega delovanja zaščitnega sredstva za les proti jajčecem hišnega kozlička Hylotrupes bajulus (Linnaeus), ko je zaščitno sredstvo uporabljeno v sklopu površinske obdelave lesa. Ta metoda se uporablja za: – v vodi netopne kemikalije, ki se preučujejo kot aktivni insekticidi; – organske formulacije, ki so dobavljene ali pripravljene v laboratoriju z redčenjem koncentratov; – organske formulacije, ki se razpršijo v vodi in so dobavljene ali pripravljene v laboratoriju z redčenjem koncentratov; ali – v vodi topne materiale, na primer sol. Metoda se uporablja ne glede na to, ali so preskušanci izpostavljeni ustreznim postopkom staranja ali ne.

General Information

• Status: Published
• Public Enquiry End Date: 09-Nov-2015
• Publication Date: 16-Aug-2016
• ICS: 71.100.50 - Wood-protecting chemicals
• Technical Committee: LLZ - Les, lesni izdelki in zaščita lesa
• Current Stage: 6060 - National Implementation/Publication (Adopted Project)
• Start Date: 02-Aug-2016
• Due Date: 07-Oct-2016
• Completion Date: 17-Aug-2016
• Ref Project: EN 46-2:2016 - Wood preservatives - Determination of the preventive action against recently hatched larvae of Hylotrupes bajulus (Linnaeus) - Part 2: Ovicidal effect (laboratory method)

Relations

• Replaces: SIST EN 46-2:2010 - Wood preservatives - Determination of the preventive action against recently hatched larvae of Hylotrupes bajulus (Linnaeus) . Part 2: Ovicidal effect (laboratory method)
• Effective Date: 01-Sep-2016
• Revised: oSIST prEN 46-2:2025 - Wood preservatives - Determination of the preventive action against recently hatched larvae of Hylotrupes bajulus (Linnaeus) - Part 2: Ovicidal effect (Laboratory method)
• Effective Date: 12-Apr-2023

Overview

EN 46-2:2016 is a CEN laboratory standard that specifies a method to determine the ovicidal effect of surface-applied wood preservatives against eggs of the house longhorn beetle, Hylotrupes bajulus (Linnaeus). The method evaluates whether eggs laid on treated timber produce viable larvae and is intended to be used alone or in combination with EN 46-1 (larvicidal/tunnelling assessment). EN 46-2 applies to a wide range of preservatives including water‑insoluble actives, organic formulations, water‑dispersible formulations and water‑soluble materials (e.g., salts). The procedure is valid whether or not test specimens have been aged.

Key topics and technical requirements

• Test principle: Treated wood panels are offered to freshly mated H. bajulus females; subsequent egg hatching and larval viability on treated surfaces are recorded. If ovicidal action is insufficient, EN 46-1 is used to assess larval mortality/tunnelling.
• Applicable products: Water‑insoluble actives, organic concentrates (as supplied or diluted), water‑dispersible formulations, and water‑soluble preservatives.
• Specimen and wood quality: Reference species is Scots pine (Pinus sylvestris); timber must be sound sapwood with limited resin and no prior chemical/thermal damage.
• Test environment and apparatus: Controlled environments are required, e.g. culturing chamber at (28 ± 2) °C / RH 70 ± 5 %, conditioning chamber at (20 ± 2) °C / RH 65 ± 5 %, testing chamber at (22 ± 2) °C / RH ≈ 70 %. Typical consumables include paraffin wax (setting point ~52–54 °C) or gelatine for sealing, grade 3 laboratory water (EN ISO 3696), Petri dishes, glass plates (≈48 × 25 mm) and standard lab balances.
• Safety and handling: Strict isolation and extraction measures for highly active or volatile products; Annex D covers environmental, health and safety precautions. Avoid hazardous solvents like benzene.
• Documentation: Test report format, culturing techniques for H. bajulus, and optional procedures (e.g., wood heartwood/sapwood differentiation) are provided in informative annexes.

Applications and who uses this standard

• Wood preservative manufacturers - to substantiate ovicidal performance claims for surface treatments.
• Independent testing laboratories - for regulatory testing, product approval and quality control.
• Certification bodies and regulators - to assess compliance with European testing protocols.
• Researchers and consultancies - studying insect resistance, formulation performance, or treatment longevity.
• Timber treatment businesses - to validate treatment efficacy against egg hatch and early larval attack.

Related standards

• EN 46-1 - Wood preservatives: larvicidal/tunnelling effect (complementary test)
• EN 73, EN 84 - Accelerated ageing procedures for treated wood
• EN ISO 3696 - Water for analytical laboratory use

Keywords: EN 46-2, wood preservatives, ovicidal effect, Hylotrupes bajulus, laboratory method, surface treatment, Pinus sylvestris, CEN, wood protection, testing standard.

Standards Content (Sample)

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Holzschutzmittel - Bestimmung der vorbeugenden Wirkung gegenüber frisch geschlüpften Larven von Hylotrupes bajulus (Linnaeus) - Teil 2: Ovizide Wirkung (Laboratoriumsverfahren)Produits de préservation du bois - Détermination de l'action préventive contre les larves récemment écloses d'Hylotrupes bajulus (Linnaeus) - Partie 2 : Effet ovicide (Méthode de laboratoire)Wood preservatives - Determination of the preventive action against recently hatched larvae of Hylotrupes bajulus (Linnaeus) - Part 2: Ovicidal effect (laboratory method)71.100.50Wood-protecting chemicalsICS:Ta slovenski standard je istoveten z:EN 46-2:2016SIST EN 46-2:2016en,fr,de01-september-2016SIST EN 46-2:2016SLOVENSKI
STANDARDSIST EN 46-2:20101DGRPHãþD

EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 46-2
June
t r s x ICS
y sä s r rä w r Supersedes EN
v xæ tã t r r {English Version
Wood preservatives æ Determination of the preventive action against recently hatched larvae of Hylotrupes Produits de préservation du bois æ Détermination de l 5action préventive contre les larves récemment écloses
Holzschutzmittel æ Bestimmung der vorbeugenden Wirkung gegenüber frisch geschlüpften Larven von This European Standard was approved by CEN on
w January
t r s yä
egulations which stipulate the conditions for giving this European Standard the status of a national standard without any alterationä Upætoædate lists and bibliographical references concerning such national standards may be obtained on application to the CENæCENELEC Management Centre or to any CEN memberä
translation under the responsibility of a CEN member into its own language and notified to the CENæCENELEC Management Centre has the same status as the official versionsä
CEN members are the national standards bodies of Austriaá Belgiumá Bulgariaá Croatiaá Cyprusá Czech Republicá Denmarká Estoniaá Finlandá Former Yugoslav Republic of Macedoniaá Franceá Germanyá Greeceá Hungaryá Icelandá Irelandá Italyá Latviaá Lithuaniaá Luxembourgá Maltaá Netherlandsá Norwayá Polandá Portugalá Romaniaá Slovakiaá Sloveniaá Spainá Swedená Switzerlandá Turkey andUnited Kingdomä
EUROPEAN COMMITTEE FOR STANDARDIZATION COMITÉ EUROPÉEN DE NORMALISATION EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre:
Avenue Marnix 17,
B-1000 Brussels
t r s x CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Membersä Refä Noä EN
v xæ tã t r s x ESIST EN 46-2:2016

Example of a test report . 16 Annex B (informative)
Technique for culturing Hylotrupes bajulus (Linnaeus) . 18 B.1 General . 18 B.2 Obtaining parent beetles . 18 B.3 Mating . 18 SIST EN 46-2:2016

Differentiation of heartwood and sapwood in Pinus species . 21 C.1 Principle . 21 C.2 Reagents . 21 C.3 Apparatus . 21 C.4 Procedure . 21 Annex D (informative)
Environmental, health and safety precautions within chemical/biological laboratory . 22 Bibliography . 23
introduction of new harmonised specifications for wood quality; b)
option to omit control test specimens treated with the solvent or diluents only when the solvent or diluents is water of drinking quality. The standard EN 46 is composed of two parts: — EN 46-1, Wood preservatives
wã Application by surface treatment
— EN 46-2, Wood preservatives
EN 46 consists of two parts to enable preventive action of wood preservatives, against recently hatched larvae of Hylotrupes bajulus, which are intended to be applied by surface treatment; Part 1 is required to determine the larvicidal effect of preservatives and Part 2 is required to determine the ovicidal action of the preservatives after egg-laying of young females. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. SIST EN 46-2:2016

EN 73, Wood preservatives — Accelerated ageing of treated wood prior to biological testing — Evaporative ageing procedure EN 84, Wood preservatives — Accelerated ageing of treated wood prior to biological testing — Leaching procedure EN ISO 3696, Water for analytical laboratory use —
3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 representative sample sample having its physical and/or chemical characteristics identical to the volumetric average characteristics of the total volume being sampled [SOURCE: EN 1001-2:2005, 4.71] SIST EN 46-2:2016

1) In southern European countries the pine species most frequently infested by Hylotrupes bajulus may be used as an alternative, provided that the suitability of the species for use in the tests specified in this standard has been demonstrated in all aspects (development of larvae, resistance of impregnation, etc.). SIST EN 46-2:2016

2) For special tests, test specimens may be obtained according to a given series. As a result, it may be preferable to take test specimens from pretreated strips. Where pretreated strips are used details should be included in the test report. SIST EN 46-2:2016
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