SIST EN ISO 12966-2:2011

Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 2: Preparation of methyl esters of fatty acids (ISO 12966-2:2011)

This part of ISO 12966 specifies methods of preparing the methyl esters of fatty acids. It includes methods for preparing fatty acid methyl esters from animal and vegetable fats and oils, fatty acids and soaps. To cover different requirements four methylation methods are specified, namely:
a) a “rapid” transmethylation procedure under alkaline conditions;
b) a “general” transmethylation/methylation procedure under sequential alkaline and acid conditions;
c) a BF3 transmethylation procedure;
d) an alternative procedure using acid-catalysed transmethylation of glycerides.
Methyl esters so produced are used in various analytical procedures requiring such derivatives, e.g. gas-liquid chromatography (GLC), thin-layer chromatography (TLC), and infrared spectrometry (IR).

Tierische und pflanzliche Fette und Öle - Gaschromatographie von Fettsäuremethylestern - Teil 2: Herstellung von Fettsäuremethylestern (ISO 12966-2:2011)

Diese Internationale Norm legt Verfahren zur Herstellung der Methylester von Fettsäuren fest.
Sie bezieht Verfahren für die Herstellung von Fettsäuremethylestern aus tierischen und pflanzlichen Fetten
und Ölen, Fettsäuren und Seifen mit ein. Um den verschiedenen Anforderungen gerecht zu werden, sind vier
Methylierungsverfahren festgelegt, insbesondere:
- ein „Schnellverfahren“ der Transmethylierung unter alkalischen Bedingungen (siehe 4.2);
- ein „allgemeines“ Transmethylierungs-/Methylierungsverfahren unter aufeinanderfolgenden alkalischen
und sauren Bedingungen (siehe 4.3);
- ein BF3-Transmethylierungsverfahren (siehe 4.4);
- ein alternatives Verfahren mit säurekatalysierter Transmethylierung der Glyceride (siehe 4.5).
Die so gewonnenen Methylester werden für verschiedene analytische Verfahren verwendet, bei denen diese
Derivate erforderlich sind, z. B. Gas-flüssig-Chromatographie (GC), Dünnschichtchromatographie (DC) und
Infrarotspektrometrie (IR).

Corps gras d'origines animale et végétale - Chromatographie en phase gazeuse des esters méthyliques d'acides gras - Partie 2: Préparation des esters méthyliques d'acides gras (ISO 12966-2:2011)

L'ISO 12966-2:2011 spécifie des méthodes de préparation des esters méthyliques d'acides gras.
Elle comprend des méthodes de préparation des esters méthyliques d'acides gras à partir de corps gras d'origines animale et végétale, d'acides gras et de savons. Afin de répondre à différentes exigences, quatre méthodes de méthylation sont spécifiées, à savoir: a) une méthode de transméthylation «rapide» en conditions alcalines; b) une méthode de transméthylation/méthylation «générale» en conditions alcalines et acides séquentielles; c) une méthode de transméthylation au BF3; d) une méthode alternative reposant sur la transméthylation par catalyse acide de glycérides.
Les esters méthyliques ainsi obtenus sont utilisés dans diverses méthodes d'analyse exigeant des produits dérivés de ce type, par exemple la chromatographie en phase gazeuse, la chromatographie sur couche mince et la spectrométrie infrarouge.

Rastlinske in živalske maščobe in olja - Plinska kromatografija metilnih estrov maščobnih kislin - 2. del: Priprava metilnih estrov maščobnih kislin (ISO 12966-2:2011)

Ta del ISO 12966 določa metode priprave metilnih estrov maščobnih kislin. Zajema metode za pripravo metilnih estrov maščobnih kislin iz živalskih in rastlinskih maščob in olj, maščobnih kislin in mil. Da bi zajeli različne zahteve, so določene štiri metode metilacije, in sicer:
a) »hiter« postopek transmetilacije v alkalnih okoliščinah;
a) »splošni« postopek transmetilacije/metilacije v zaporednih alkalnih in kislih okoliščinah;
c) postopek transmetilacije BF3;
d) alternativni postopek z uporabo transmetilacije gliceridov, kataliziranih s kislino.
Metlini estri, proizvedeni tako, se uporabljajo v različnih analitičnih postopkih, kjer so potrebni taki derivati, npr. plinska-tekočinska kromatografija (GLC), tankoplastna kromatografija (TLC) in infrardeča spektrometrija (IR).

General Information

• Status: Withdrawn
• Publication Date: 16-Jun-2011
• Withdrawal Date: 05-Jun-2017
• ICS: 67.200.10 - Animal and vegetable fats and oils
• ICS: 71.040.50 - Physicochemical methods of analysis
• Technical Committee: KŽP - Agricultural food products
• Current Stage: 9900 - Withdrawal (Adopted Project)
• Start Date: 06-Jun-2017
• Due Date: 29-Jun-2017
• Completion Date: 06-Jun-2017
• Ref Project: EN ISO 12966-2:2011 - Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 2: Preparation of methyl esters of fatty acids (ISO 12966-2:2011)

Relations

• Replaces: SIST EN ISO 5509:2001 - Animal and vegetable fats and oils - Preparation of methyl esters of fatty acids (ISO 5509:2000)
• Effective Date: 01-Jul-2011
• Replaced By: SIST EN ISO 12966-2:2017 - Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 2: Preparation of methyl esters of fatty acids (ISO 12966-2:2017)
• Effective Date: 08-Jun-2022
• Replaced By: SIST EN ISO 12966-2:2017 - Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 2: Preparation of methyl esters of fatty acids (ISO 12966-2:2017)
• Effective Date: 01-Jul-2017

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN ISO 12966-2:2011
01-julij-2011
1DGRPHãþD
SIST EN ISO 5509:2001
5DVWOLQVNHLQåLYDOVNHPDãþREHLQROMD3OLQVNDNURPDWRJUDILMDPHWLOQLKHVWURY
PDãþREQLKNLVOLQGHO3ULSUDYDPHWLOQLKHVWURYPDãþREQLKNLVOLQ,62

Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters -
Part 2: Preparation of methyl esters of fatty acids (ISO 12966-2:2011)
Tierische und pflanzliche Fette und Öle - Gaschromatographie von
Fettsäuremethylestern - Teil 2: Herstellung von Fettsäuremethylestern (ISO 12966-
2:2011)
Corps gras d'origines animale et végétale - Chromatographie en phase gazeuse des
esters méthyliques d'acides gras - Partie 2: Préparation des esters méthyliques d'acides
gras (ISO 12966-2:2011)
Ta slovenski standard je istoveten z: EN ISO 12966-2:2011
ICS:
67.200.10 5DVWOLQVNHLQåLYDOVNH Animal and vegetable fats
PDãþREHLQROMD and oils
71.040.50 Fizikalnokemijske analitske Physicochemical methods of
metode analysis
SIST EN ISO 12966-2:2011 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN ISO 12966-2:2011

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SIST EN ISO 12966-2:2011


EUROPEAN STANDARD
EN ISO 12966-2

NORME EUROPÉENNE

EUROPÄISCHE NORM
February 2011
ICS 67.200.10 Supersedes EN ISO 5509:2000
English Version
Animal and vegetable fats and oils - Gas chromatography of
fatty acid methyl esters - Part 2: Preparation of methyl esters of
fatty acids (ISO 12966-2:2011)
Corps gras d'origines animale et végétale - Tierische und pflanzliche Fette und Öle -
Chromatographie en phase gazeuse des esters Gaschromatographie von Fettsäuremethylestern - Teil 2:
méthyliques d'acides gras - Partie 2: Préparation des Herstellung von Fettsäuremethylestern (ISO 12966-2:2011)
esters méthyliques d'acides gras (ISO 12966-2:2011)
This European Standard was approved by CEN on 14 February 2011.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same
status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland,
Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2011 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 12966-2:2011: E
worldwide for CEN national Members.

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SIST EN ISO 12966-2:2011
EN ISO 12966-2:2011 (E)
Contents Page
Foreword .3

2

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SIST EN ISO 12966-2:2011
EN ISO 12966-2:2011 (E)
Foreword
This document (EN ISO 12966-2:2011) has been prepared by Technical Committee ISO/TC 34 "Food
products" in collaboration with Technical Committee CEN/TC 307 “Oilseeds, vegetable and animal fats and
oils and their by-products - Methods of sampling and analysis” the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by August 2011, and conflicting national standards shall be withdrawn at
the latest by August 2011.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 5509:2000.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech
Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia,
Lithuania, Luxembourg, Malta, Netherlan
...