This European Standard specifies a method for the determination of sucralose in foodstuffs by high performance liquid chromatography (HPLC) by means of elution from a reversed-phased (RP) column using aqueous methanol, followed by RI detection [1]. This method has been validated in an inter-laboratory study via the analysis of sucralose (from 83 mg/kg to 737 mg/kg) in spiked samples of ketchup, mayonnaise, biscuits, yoghurt, instant beverage powder and sweets.
For further information on the validation results, see Annex A.

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This European Standard specifies a method for the simultaneous determination of nine sweeteners in beverages and canned or bottled fruits by high performance liquid chromatography (HPLC) with evaporative light scattering detection (HPLC-ELSD). This method has been validated in an interlaboratory study via the analysis of spiked samples on the following matrices:
-   acesulfame-K (ACS-K) (from 38,3 mg/l to 383,5 mg/l) in beverages and (from 38,4 mg/kg to 391,3 mg/kg) in canned fruits;
-   alitame (ALI) (from 31,1 mg/l to 114,5 mg/l) in beverages and (from 36 mg/kg to 175,2 mg/kg) in canned fruits;
-   aspartame (ASP) (from 38,1 mg/l to 702 mg/l) in beverages and (from 37,2 mg/kg to 1 120,2 mg/kg) in canned fruits;
-   cyclamic acid (CYC) (from 28,3 mg/l to 307,2 mg/l) in beverages and (from 27,5 mg/kg to 1 100,6 mg/kg) in canned fruits;
-   dulcin (DUL) (from 55,0 mg/l to 115,1 mg/l) in beverages and (from 49,8 mg/kg to 172,6 mg/kg) in canned fruits;
-   neotame (NEO) (from 37,6 mg/l to 115,3 mg/l) in beverages and (from 37,3 mg/kg to 173,7 mg/kg) in canned fruits;
-   neohesperidine dihydrochalcone (NHDC) (from 31,4 mg/l to 59,3 mg/l) in beverages and (from 35,3 mg/kg to 59,3 mg/kg) in canned fruits;
-   saccharin (SAC) (from 36,2 mg/l to 87,6 mg/l) in beverages and (from 44,3 mg/kg to 235,3 mg/kg) in canned fruits;
-   sucralose (SCL) (from 36,8 mg/l to 346,8 mg/l) in beverages and (from 35,3 mg/kg to 462,4 mg/kg) in canned fruits.
For further information on the validation results see Annex C.
NOTE   The method has been fully validated [1] through collaborative trial, according to the IUPAC Harmonised Protocol [2], on analyte-matrix combinations of all nine sweeteners in beverages and canned or bottled fruits.

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This Technical Specification (prCEN/TS 15606:2009) specifies a high performance liquid chromatographic (HPLC) method for the determination of acesulfame-K, aspartame, neohesperidine-dihydrochalcone and saccharin in foodstuffs. The method has been fully validated [1] for the dialysis procedure through collaborative trial, according to the IUPAC Harmonised Protocol [2], on the following analyte matrix combinations:
   acesulfame-K and aspartame in water-based drink, fruit-based drink, cheesecake with biscuit base, canned soup and instant chocolate drink;
   saccharin in water-based drink, fruit-based drink, cheesecake with biscuit base and canned soup;
   neohesperidine-dihydrochalcone in water-based drink, fruit-based drink and canned soup.

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This European Standard specifies an HPLC-method for the determination of isomalt and other polyols such as lactitol, maltitol, mannitol, sorbitol and xylitol in foodstuffs. Chemically isomalt is described as a mixture of 6-O-a-D-glucopyranosyl-D-sorbitol (1,6-GPS) and 1-O-a-D-glucopyranosyl-D-mannitol (1,1-GPM).
The method has been validated in a collaborative study for isomalt (sum of GPS and GPM) on cookies, chewing gum, chocolate and on hard candies. Validation data for GPS and GPM are given in Clause 8, Annex A, Table A.1 and A.2
The determination of the other sugar alcohols has been (also) validated in a further collaborative study using the same method. The samples were pudding (lactitol, mannitol, xylitol), cookies (lactitol, maltitol, mannitol, sorbitol and xylitol), hard candies (lactitol, mannitol, xylitol, sorbitol) and chewing gum (maltitol, mannitol, sorbitol). Validation data are given in Clause 8 and Annex A, Tables A.3 to A.7.

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This Technical Specification specifies an HPLC-method for the determination of neohesperidin-dihydrochalcon (NHDC) in foodstuffs.
It has been validated in a collaborative test with cherry yoghurt containing 42,7 mg/kg and on a multi vitamin drink containing 35,6 mg/l NHDC [1].
The method has been successfully applied to a range of other foods including marzipan, bakery products, cream, custard powder, chocolate, ice cream.

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This European Standard specifies a high performance liquid chromatographic (HPLC) method for the determination of acesulfame-K, aspartame, and saccharin, see (1), (2) and (3). It also allows the determination of caffeine, sorbic acid and benzoic acid in foodstuffs. Interlaboratory tests have been carried out with acesulfame-K in marzipan, yogurt, fruit yogurt, orange juice beverage, cola, cream and jam, with aspartame in marzipan, fruit yogurt, orange juice beverage, orange flavoured beverage, cola, jam, and preparation for flan, and with sodium saccharin in marzipan, yogurt, fruit yogurt, orange juice, ...

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This draft European Standard specifies a high performance liquid chromatographic (HPLC) method for the determination of sodium cyclamate in foodstuffs (1), (2), (3). The method has been validated in an inter-laboratory test according to ISO 5725:1986 (4) which has been carried out with lemonade, orange juice beverage, fruit yogurt and spray cream. In the addition to the matrices investigated in the inter-laboratory test, experiences have shown that the method is also applicable to various foodstuffs, such as gherkins, canned morello cherries, pineapple, orange nectar, apricot jam, ...

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This European Standard specifies a spectrometric method for the determination of sodium saccharin and saccharin content in solid table top sweetener preparations prepared from cyclamate/saccharin or saccharin. An inter-laboratory test has been carried out on sweetener tablets.

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This European Standard specifies a high performance liquid chromatography (HPLC) method for the determination of aspartame in table top sweetener preparations. An inter-laboratory test has been carried out on sweetener tablets.

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This European Standard specifies a spectrometric method for the determination of acesulfame K in solid table top sweetener preparations containing it. an inter-laboratory test has been carried out on sweetener tablets.

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This European Standard specifies a high performance liquid chromatography (HPLC) method for the determination of sodium cyclamate and saccharin in liquid table top sweetener preparations. It also allows the determination of sorbic acid in liquid table top sweetener preparations. An interlaboratory test has been carried out with liquid table top sweetener preparation.

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