This International Standard specifies the production and sanitary requirements for royal jelly
and establishes a series of organoleptic and chemical test methods to control royal jelly quality. It
also specifies the requirements of transport, storage, packaging and marking for royal jelly. This
International Standard applies to the royal jelly production (collecting, preliminary processing and
packaging) and trade links. This International Standard is not applicable to royal jelly products in
which other foods are mixed.

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ISO 12824:2016 specifies the production and sanitary requirements for royal jelly and establishes a series of organoleptic and chemical test methods to control royal jelly quality. It also specifies the requirements of transport, storage, packaging and marking for royal jelly. This International Standard applies to the royal jelly production (collecting, preliminary processing and packaging) and trade links. This International Standard is not applicable to royal jelly products in which other foods are mixed.

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This European Standard specifies a method for the simultaneous determination of nine sweeteners in beverages and canned or bottled fruits by high performance liquid chromatography (HPLC) with evaporative light scattering detection (HPLC-ELSD). This method has been validated in an interlaboratory study via the analysis of spiked samples on the following matrices:
- acesulfame-K (ACS-K) (from 38,3 mg/l to 383,5 mg/l) in beverages and (from 38,4 mg/kg to 391,3 mg/kg) in canned fruits;
- alitame (ALI) (from 31,1 mg/l to 114,5 mg/l) in beverages and (from 36 mg/kg to 175,2 mg/kg) in canned fruits;
- aspartame (ASP) (from 38,1 mg/l to 702 mg/l) in beverages and (from 37,2 mg/kg to 1 120,2 mg/kg) in canned fruits;
- cyclamic acid (CYC) (from 28,3 mg/l to 307,2 mg/l) in beverages and (from 27,5 mg/kg to 1 100,6 mg/kg) in canned fruits;
- dulcin (DUL) (from 55,0 mg/l to 115,1 mg/l) in beverages and (from 49,8 mg/kg to 172,6 mg/kg) in canned fruits;
- neotame (NEO) (from 37,6 mg/l to 115,3 mg/l) in beverages and (from 37,3 mg/kg to 173,7 mg/kg) in canned fruits;
- neohesperidine dihydrochalcone (NHDC) (from 31,4 mg/l to 59,3 mg/l) in beverages and (from
35,3 mg/kg to 59,3 mg/kg) in canned fruits;
- saccharin (SAC) (from 36,2 mg/l to 87,6 mg/l) in beverages and (from 44,3 mg/kg to 235,3 mg/kg) in canned fruits;
- sucralose (SCL) (from 36,8 mg/l to 346,8 mg/l) in beverages and (from 35,3 mg/kg to 462,4 mg/kg) in canned fruits. For further information on the validation see Clause 8 and Annex C.

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This European Standard specifies a method for the simultaneous determination of nine sweeteners in beverages and canned or bottled fruits by high performance liquid chromatography (HPLC) with evaporative light scattering detection (HPLC-ELSD). This method has been validated in an interlaboratory study via the analysis of spiked samples on the following matrices:
-   acesulfame-K (ACS-K) (from 38,3 mg/l to 383,5 mg/l) in beverages and (from 38,4 mg/kg to 391,3 mg/kg) in canned fruits;
-   alitame (ALI) (from 31,1 mg/l to 114,5 mg/l) in beverages and (from 36 mg/kg to 175,2 mg/kg) in canned fruits;
-   aspartame (ASP) (from 38,1 mg/l to 702 mg/l) in beverages and (from 37,2 mg/kg to 1 120,2 mg/kg) in canned fruits;
-   cyclamic acid (CYC) (from 28,3 mg/l to 307,2 mg/l) in beverages and (from 27,5 mg/kg to 1 100,6 mg/kg) in canned fruits;
-   dulcin (DUL) (from 55,0 mg/l to 115,1 mg/l) in beverages and (from 49,8 mg/kg to 172,6 mg/kg) in canned fruits;
-   neotame (NEO) (from 37,6 mg/l to 115,3 mg/l) in beverages and (from 37,3 mg/kg to 173,7 mg/kg) in canned fruits;
-   neohesperidine dihydrochalcone (NHDC) (from 31,4 mg/l to 59,3 mg/l) in beverages and (from 35,3 mg/kg to 59,3 mg/kg) in canned fruits;
-   saccharin (SAC) (from 36,2 mg/l to 87,6 mg/l) in beverages and (from 44,3 mg/kg to 235,3 mg/kg) in canned fruits;
-   sucralose (SCL) (from 36,8 mg/l to 346,8 mg/l) in beverages and (from 35,3 mg/kg to 462,4 mg/kg) in canned fruits.
For further information on the validation results see Annex C.
NOTE   The method has been fully validated [1] through collaborative trial, according to the IUPAC Harmonised Protocol [2], on analyte-matrix combinations of all nine sweeteners in beverages and canned or bottled fruits.

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This European Standard specifies a high performance liquid chromatography (HPLC) method for the determination of sodium cyclamate and saccharin in liquid table top sweetener preparations. It also allows the determination of sorbic acid in liquid table top sweetener preparations. An interlaboratory test has been carried out with liquid table top sweetener preparation.

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This European Standard specifies a spectrometric method for the determination of sodium saccharin and saccharin content in solid table top sweetener preparations prepared from cyclamate/saccharin or saccharin. An inter-laboratory test has been carried out on sweetener tablets.

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This European Standard specifies a spectrometric method for the determination of acesulfame K in solid table top sweetener preparations containing it. an inter-laboratory test has been carried out on sweetener tablets.

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This European Standard specifies a high performance liquid chromatography (HPLC) method for the determination of aspartame in table top sweetener preparations. An inter-laboratory test has been carried out on sweetener tablets.

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This European Standard specifies a high performance liquid chromatography (HPLC) method for the determination of sodium cyclamate and saccharin in liquid table top sweetener preparations. It also allows the determination of sorbic acid in liquid table top sweetener preparations. An interlaboratory test has been carried out with liquid table top sweetener preparation.

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This European Standard specifies a spectrometric method for the determination of acesulfame K in solid table top sweetener preparations containing it. an inter-laboratory test has been carried out on sweetener tablets.

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This European Standard specifies a spectrometric method for the determination of sodium saccharin and saccharin content in solid table top sweetener preparations prepared from cyclamate/saccharin or saccharin. An inter-laboratory test has been carried out on sweetener tablets.

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This European Standard specifies a high performance liquid chromatography (HPLC) method for the determination of aspartame in table top sweetener preparations. An inter-laboratory test has been carried out on sweetener tablets.

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This document specifies a method for the determination of the ratio of stable isotopes of carbon (13C/12C) of sugars contained in honey by using liquid chromatography coupled to an isotope ratio mass spectrometer (LC-IRMS) for compound separation and subsequent determination of the 13C/12C ratio of mono-, di-, and trisaccharides. These ratios can be used to assess honey authenticity by comparing them to published guidance values of genuine honey as the 13C/12C ratios of sugars of genuine honey and sugars contained in adulterants (syrups made from starch-rich plants or from sugar cane or sugar beet) differ to a certain extent. The compliance assessment process is not part of this document.

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This document specifies a method for the determination of the ratio of stable isotopes of carbon (13C/12C) of sugars contained in honey by using liquid chromatography coupled to an isotope ratio mass spectrometer (LC-IRMS) for compound separation and subsequent determination of the 13C/12C ratio of mono-, di-, and trisaccharides. These ratios can be used to assess honey authenticity by comparing them to published guidance values of genuine honey as the 13C/12C ratios of sugars of genuine honey and sugars contained in adulterants (syrups made from starch-rich plants or from sugar cane or sugar beet) differ to a certain extent. The compliance assessment process is not part of this document.

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