ASTM E211-82(1999)e1

Standard Specification for Cover Glasses and Glass Slides for Use in Microscopy

SCOPE
1.1 This specification describes glass covers and slides for use in routine microscopy.

General Information

• Status: Historical
• Publication Date: 09-Dec-1999
• ICS: 37.020 - Optical equipment
• Technical Committee: E41 - Laboratory Apparatus
• Drafting Committee: E41.01 - Laboratory Ware and Supplies

Relations

• Replaced By: ASTM E211-82(2005) - Standard Specification for Cover Glasses and Glass Slides for Use in Microscopy
• Effective Date: 01-May-2005

Standards Content (Sample)

NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
e1
Designation: E 211 – 82 (Reapproved 1999)
Standard Specification for
Cover Glasses and Glass Slides for Use in Microscopy
This standard is issued under the fixed designation E 211; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (e) indicates an editorial change since the last revision or reapproval.
e NOTE—Keywords were added editorially in March 2000.
1. Scope 3.2.2 Rectangular—24 by 55 mm, 35 by 50 mm, 45 by 50
mm, 20 by 26 mm and 20 by 40 mm.
1.1 This specification describes glass covers and slides for
3.2.3 Circular—18, 22 or 25 mm in diameter.
use in routine microscopy.
3.2.4 Thickness—Thickness Number 0, 0.08 to 0.13 mm.
2. Glass
3.3 Tolerance—Tolerance on dimensions other than thick-
ness shall be 60.5 mm.
2.1 Cover Glass—Glass shall be colorless, transparent
3.4 Blood Counting Chambers—The dimensions of cover
glass, sufficiently free of pits, bubbles, or cloudiness so as not
glasses for blood counting chambers shall be as follows:
to interfere with end-use performance. Such defects shall be
A A B
Length, mm width, mm Thickness, mm
regarded as major defects and limited accordingly. Inspection
20 21 0.4 or 0.6
shall be by the unaided eye in a good light viewing a single
20 26 0.4 or 0.6
cover glass at a time. Refractive index at sodium D line (589.3
25 28 0.4 or 0.6
nm) shall be 1.523 6 0.005.
A
The tolerance on length and width shall be 61.0 mm.
2.2 Glass Slides—Glass slides covered by this specification
B
The tolerance on thickness shall be 6 0.06 mm.
shall consist of colorless, transparent glass which may have a
slight greenish tint when viewed edgewise. It shall be suffi- 4. Planeness
ciently free of pits, nicks, bubbles, striae, scratches, and
4.1 General Use—Cover glass may be expected to pass the
cloudiness when observed by the unaided eye so as not to
test as described in 14.1.
impair end-use performance. Index of refraction at the sodium
4.2 Blood Counting Chambers—Cover glass shall be plane
D line (589.3 nm) shall be 1.50 to 1.54.
within0.002mmonbothsidesandshallpasstheplanenesstest
as described in 16.2.
COVER GLASSES
5. Corrosion Resistance
3. Dimensions
5.1 While no corrosion tests for cover glasses are hereby
3.1 General Use—The dimensions of cover glass for gen-
required, when desired for critical use, use the test in 14.2.
eral use shall be as follows:
3.1.1 Square—22 mm on a side.
GLASS SLIDES
3.1.2 Rectangular—22 by 30, 22 by 40, 22 by 50, or 22 by
60 mm and 24 by 30, 24 by 40, 24 by 50, and 24 by 60 mm. 6. Dimensions
3.1.3 Thickness—The thickness shall be as follows:
6.1 General Use:
Number Thickness, mm
6.1.1 Size—75.5 by 25.5 6 0.8 mm.
1 0.13 to 0.17
6.1.2 Thickness A—1.0 6 0.15 mm.
A
1 ⁄2 0.16 to 0.19
Thickness B—1.2 6 0.15 mm.
2 0.17 to 0.25
6.2 Darkfield and Interference Microscopy:
A
Specified as optimum by most microscope manufacturers.
6.2.1 Size—75.5 by 25.5 6 0.8 mm.
3.2 Limited Use—The following sizes while recognized,
6.2.2 Thickness C—1.20 6 0.005 mm.
find limited use and are generally not as readily available.
Thickness D—0.90 6 0.075 mm.
3.2.1 Square—18, 24 and 25 mm on a side.
Thickness E—1.506 0.05 mm.
6.3 Embryology—37.5 6 0.8 or 50.56 0.8 by 75.5 6 0.8
mm.
This specification is under the jurisdiction of ASTM Committee E-41 on
6.3.1 Thickness—Same as either thickness A or thickness B
Laboratory Apparatus and is the direct responsibility of Subcommittee E41.01 on
in 6.1 on general-use glass slides.
Glass Apparatus.
6.4 Petrography:
Current edition approved Jan. 29, 1982. Published March 1982. Originally
published as E 211 – 63 T. Last previous edition E 211 – 70. 6.4.1 Size—25.5 by 45.5, 27 by 46, or 28 by 48 6 0.8 mm.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.
E211
6.4.2 Thickness—Same as thickness A or B under general- normal vision without further magnification. Examine cover
use slides. glasses in good light in front of a dark surface. Examine
general-use slides with daylight or diffuse uniform artificial
7. Planeness and Parallelism
light against a uniform dark background. Examine darkfield
and interference microscopy slides with a 103 magnifier in a
7.1 General Use—No requirements.
7.2 Darkfield, Interference, Embryology, Petrography—No light beam of at least 25 fc against a black background.
requirements.
14. Planeness
8. Corrosion Resistance
14.1 The number of cover glasses specified in Table 1 shall
be stacked on a flat surface. The height of the stack shall be
8.1 General Use and Darkfield and Interference
measured within 6 0.05 mm.Athin stiff aluminum plate cut to
Microscopy—The slides shall not be adversely affected by
the size of the cover glass is to be placed on top of the stack.
boilingnor shall they exhibit any pink color in the solubility
Aweight is placed on top of the plate so that the total mass of
test.
the aluminum plate plus the weight is 100 g.Again, the height
8.2 Embryology, Petrography—No requirements.
of the stack is to be measured to within 6 0.05 mm. The
9. Workmanship
difference between the unloaded and loaded readings is the
compression. Compression shall not exceed the amount shown
9.1 All edges of all glass slides shall be polished or ground.
in the Table 1.
They shall pass the wettability tests in Section 17. General-use
14.2 Test planeness against an optical flat, using the inter-
microscope slides shall be smooth and free from nicks or
ferometric method described in National Bureau of Standard
blemishes on the short edge which might impair their service-
Circular 507.
ability for blood smear work.
9.2 Frosted End—One end of one or both surfaces of
15. Thickness Variation
microscope slides may be frosted to allow marking with a No.
15.1 Permissible thickness variation in any one specimen of
2 pencil or ballpoint pen for a distance of 19 6 3 mm from the
cover glass is as follows:
end. Alternatively, both ends of the microscope slide may be
15.1.1 For sizes up to and including 25 mm in any one
frosted for a distance of 9 6 2 mm.
dimension, not more than 1 % of the specimens measured shall
GENERAL
show a greater variation than 0.015 mm in any one specimen.
15.1.2 For sizes above 25 mm in any one dimension, not
10. Sampling
more than 1 % of the specimens measured shall show a greater
10.1 The cover g
...