E30 - Forensic Sciences

SIGNIFICANCE AND USE
4.1 This practice is intended for use as a sampling technique within a general scheme for the analysis of ignitable liquids and ignitable liquid residues from fire debris samples in accordance with Guide E3245.  
4.2 Headspace samples obtained using this practice are screened using a gas chromatograph with a flame ionization detector (GC-FID) or analyzed using a gas chromatograph with a mass spectrometer (GC-MS, refer to Test Method E1618).  
4.3 This practice is most applicable for sampling light to medium range ignitable liquids.3, 4, 5, 6 It is not capable of sufficient recovery of heavy range ignitable liquids to support accurate analysis using Test Method E1618.
Note 1: When present in high concentrations, highly volatile compounds can saturate the headspace, inhibiting the recovery of less volatile compounds by this practice. This skewed recovery can lead to the detection or identification of only the more volatile compounds in the sample.  
4.4 This practice is useful for sampling fire debris to screen for the presence of ignitable liquid residues prior to extraction with other techniques, such as those described in Practices E1386, E1412, E1413, and E2154, and E3189.  
4.4.1 This practice is less capable of recovering limited quantities of ignitable liquids than Practices E1386, E1412, E1413, E2154, and E3189, particularly for heavy range compounds.  
4.5 This practice only removes a small aliquot of the headspace vapor from a closed container; therefore, the fire debris sample remains in approximately the same condition in which it was submitted, and reanalysis using a new headspace sample, or a different sampling technique, is possible. However, removing multiple headspace samples continually reduces the concentration of ignitable liquid vapors, if originally present, and can eventually result in non-recovery by static headspace sampling.
Note 2: The headspace sample collected using this practice is consumed in the subsequent screening by GC-FID o...
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1.1 This practice describes the procedure for removing a vapor sample from the headspace of a fire debris container for the purpose of detecting or identifying ignitable liquid residues.  
1.2 Separation and concentration procedures are listed in the referenced documents. (See Practices E1386, E1412, E1413, E2154, and E3189.)  
1.3 This practice is intended for use by competent forensic science practitioners with the requisite formal education, discipline-specific training (see Practice E2917), and demonstrated proficiency to perform forensic casework.  
1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
3.1 These terms have application to forensic practice.  
3.1.1 For ASTM International standards, the standard designation is followed by a dash and a two-digit year designation in bold type, for example, E2161 - 19. The year citation references the year of publication of the standard from which the entry is taken, not necessarily the current year of publication of the standard.  
3.1.2 Citations from other than ASTM International standards may include an abbreviation and the standard number followed by a four-digit year designation in bold type, for example, ISO 9000:2015. The year citation references the year of publication of the standard from which the entry is taken. Such standards may also be referenced by a name followed by a year designation, for example, IUPAC Gold Book 2020 (1).5 Abbreviations are detailed under, “All terms sourced from other standards than Committee E30 standards are listed in Section 2.”  
3.1.3 For entries followed simply by a reference, for example, ISO 9000:2015 or E456 - 17, the reader can assume that the entry is accurately copied from the reference with no modifications except for ASTM International format conventions. For entries that are slightly modified versions of something from a known source, reference citations read, “Based upon definition by…” Following the “by” is the source name and year that the entry was taken or modified. The boldface numbers in parentheses refer to a list of references at the end of the terminology.  
3.1.4 For entries from textbooks, a reference following the entry has the name or title of the text, author(s), edition (if applicable), and the year of publication or copyright.  
3.1.5 An entry could have a definition of a term with one reference, and the discussion following the definition has a different reference.  
3.1.6 Citations from Merriam-Webster’s Online Dictionary (2) include the date retrieved from the online dictionary and the URL of the cited term and definition.  
3.1.7 Each defin...
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1.1 This terminology is the compilation of all terms used by Committee E30 on Forensic Sciences.  
1.1.1 This terminology consists mostly of definitions that are specific to forensic science and forensic practice. Meanings of the same terms used outside of forensic practice can be found in other compilations or dictionaries of general usage.  
1.1.2 The specific E30 subcommittee (SC) that has responsibility for each term is the first attribution noted, in square brackets, after the definition. The designation of the standard(s) in which the terms appear is given after the subcommittee. The wording of an entry cannot be changed without approval per the Committee E30 Bylaws. Users of this compilation should also review any SC terminology standard for more details or interpretations of these terms and their use by the SC.  
1.2 This terminology is a tool for managing the committee’s terminology. This includes finding, eliminating, and preventing redundancies in which two or more terms relating the same concept are defined in different words.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
4.1 This practice separately outlines criteria and implementation approaches for the training, continuing education, and professional development of forensic science practitioners. The use of this practice can help establish training programs designed to achieve competency in targeted disciplines. The standard also describes measures to maintain competency through continuing education/professional development.  
4.2 This practice provides a framework for extending learning opportunities to promote and achieve higher standards of professional practice in forensic science.  
4.3 This practice is not intended to be inclusive of all possible options nor to address the challenges of a particular discipline.  
4.3.1 This practice does not address proficiency testing programs or specific requirements of professional certification and licensure bodies, although the foundational requirements addressed may be essential elements for such programs.  
4.3.2 This practice is not intended to supersede requirements from professional certification and licensure bodies.
4.3.2.1 Licensing and certifying bodies in a number of fields typically impose continuing education and professional development requirements on their license or certificate holders. Such requirements are intended to encourage professionals to expand their knowledge base and keep abreast of new developments. Depending on the field, these requirements might be satisfied through internal training; completion of college, university, or extension coursework; or through attendance at conferences and seminars. Individuals in such positions should obtain and document their on-going training and development as required by their licensing or certifying body.
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1.1 This practice provides foundational requirements for the training, continuing education, and professional development of forensic science practitioners to include training criteria toward competency, documentation, implementation of training, and continuous professional development. This information is intended for forensic science service providers to help establish a training framework with program structure and content; for forensic science practitioners as they acquire and maintain their knowledge, skills, and abilities (KSAs); for subject matter experts when developing discipline specific training practices; and for training programs to manage and support the continuous development of their employees.  
1.2 This practice outlines minimum training criteria and provides general information, approaches, and resources for all disciplines. The standard would complement additional specific requirements for each forensic science discipline (for example, relevant degree programs, higher education) if developed by subject matter experts in their respective fields. Discipline specific training programs should address the content and means for developing and testing competency for each applicable topic identified in Practice E2917.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
3.1 These terms have particular application to gunshot residue analysis. In addition, several sources of definitions were used in the development of this terminology (1-7).3
SCOPE
1.1 This terminology standard is a compilation of terms and corresponding definitions that are used in gunshot residue analysis. Some legal or scientific terms that are generally understood or defined adequately in other readily available sources are included.  
1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.3 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
5.1 This guide can assist the examiner in selecting and organizing a general analytical scheme for the evaluation and documentation of physical comparisons of materials for a potential physical fit. The type and size of material influences the exact steps and equipment needed to assess the physical fit. Evaluation, documentation, and interpretation are all important parts of a physical fit examination.  
5.2 This guide addresses special considerations for physical fit analysis for glass, skeletal material, polymers, tapes and textiles.  
5.3 Foundations of physical fit examination in forensic science are described in the literature, including studies on the fractography of different materials and the use of physical fit examinations in forensic casework (1-12).  
5.4 It is not the intention of this guide to present comprehensive theories regarding the mechanism of fractures, tearing, cutting, or other methods of separation. This information is available from training courses and reference materials such as Practice C1256 and others (3-7, 13).
SCOPE
1.1 This guide covers the forensic physical fit examinations for the macroscopical and microscopical examinations of broken, torn, or separated materials for the purpose of determining whether or not they were once joined together to form a single object. This guide is intended as an overview of the process for the physical fit examination of these materials and to assist individuals in the evaluation and documentation of their physical comparisons.  
1.2 This standard is intended for use by competent forensic science practitioners with the requisite formal education, discipline-specific training (see Practice E2917), and demonstrated proficiency to perform forensic casework.  
1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
5.1 This test method is useful for the determination of elemental concentrations in the range of approximately 0.1 µgg-1 to 10 percent (%) (See Table X1.1) in soda-lime glass samples (7 and 8). A standard test method can aid in the interchange of data between laboratories and in the creation and use of glass databases.  
5.2 The determination of elemental concentrations in glass provides high discriminating value in the forensic comparison of glass fragments.  
5.3 This test method produces minimal destruction of the sample. Microscopic craters of 50 µm to 100 µm in diameter by 80 µm to 150 µm deep are left in the glass fragment after analysis. The mass removed per replicate is approximately 0.4 µg to 3 µg (6).  
5.4 Appropriate sampling techniques shall be used to account for natural heterogeneity of the materials at a microscopic scale.  
5.5 The precision, bias, and limits of detection of the method (for each element measured) shall be established during validation of the method. The measurement uncertainty of any concentration value used for a comparison shall be recorded with the concentration.  
5.6 Acid digestion of glass followed by either Inductively Coupled Plasma-Optical Emission Spectrometry (ICP-OES) or Inductively Coupled Plasma-Mass Spectrometry (ICP-MS) can also be used for trace elemental analysis of glass, and offer similar detection levels and the ability for quantitative analysis. However, these methods are destructive, and require larger sample sizes and more sample preparation (Test Method E2330).  
5.7 Micro X-Ray Fluorescence (µ-XRF) uses comparable sample sizes to those used for LA-ICP-MS with the advantage of being non-destructive of the sample. Some of the drawbacks of µ-XRF include lower sensitivity and precision, and longer analysis time (Test Method E2926).  
5.8 Scanning Electron Microscopy with Energy Dispersive Spectrometry (SEM-EDS) is also available for elemental analysis, but it is of limited use for forensic glass source d...
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1.1 This test method covers a procedure for the quantitative elemental analysis of the following seventeen elements: lithium (Li), magnesium (Mg), aluminum (Al), potassium (K), calcium (Ca), iron (Fe), titanium (Ti), manganese (Mn), rubidium (Rb), strontium (Sr), zirconium (Zr), barium (Ba), lanthanum (La), cerium (Ce), neodymium (Nd), hafnium (Hf) and lead (Pb) through the use of laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) for the forensic comparison of glass fragments. The potential of these elements to provide the best discrimination among different sources of soda-lime glasses has been published elsewhere (1-5).2 Silicon (Si) is also monitored for use as a normalization standard. Additional elements may be added as needed, for example, tin (Sn) can be used to monitor the orientation of float glass fragments.  
1.2 The method only consumes approximately 0.4 µg to 3 µg of glass per replicate and is suitable for the analysis of full thickness samples as well as irregularly shaped fragments as small as 0.1 mm by 0.1 mm by 0.2 mm (6) in dimension. The concentrations of the elements listed above range from the low parts per million (µgg-1) to percent (%) levels in soda-lime glass, the most common type encountered in forensic cases. This standard method can be applied for the quantitative analysis of other glass types; however, some modifications in the reference standard glasses and the element menu may be required.  
1.3 This standard is intended for use by competent forensic science practitioners with the requisite formal education, discipline-specific training (see Practice E2917), and demonstrated proficiency to perform forensic casework.  
1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the respo...

SIGNIFICANCE AND USE
5.1 The overarching goals of the forensic analysis of geological materials include (A) identification of an unknown material (see 11.3), (B) analysis of soils, sediments, or rocks to restrict their possible geographic origins as part of a provenance analysis (see 11.4), and (C) comparison of two or more samples to assess if they could have originated from the same source or to exclude a common source based on observation of exclusionary differences (see 11.5). XRD is only one analytical method that can be applied to the evidentiary samples in service of these distinct goals. Guidance for the analysis of forensic geological materials can be found in Refs (2-4).  
5.2 Within the analytical scheme of geological materials, XRD analysis is used to: identify the crystalline components within a sample; identify the crystalline components separated from a mixture, typically clay-sized material (see 8.8), or a selected particle class for which additional analysis is needed (see 8.11); or compare two or more samples based on the identified crystalline phases or diffraction patterns (see 11.5).  
5.2.1 Non-destructive XRD analysis can be performed in situ on geological material adhering to a substrate (see 8.12.3).  
5.2.2 The most common forensic applications of XRD to geological materials are (A) identification or confirmation of a selected phase or fraction of a sample (see 8.12), (B) identification of minerals in the clay-sized fractions of soils (see 8.8), and (C) identification of the phases of the hydrated cement component of concrete or mortar.  
5.3 This guide is intended to be used with other methods of analysis (for example, polarized light microscopy, scanning electron microscopy, palynology) within a more comprehensive analytical scheme for the forensic analysis or comparison of geological materials.  
5.3.1 Comprehensive criteria for forensic comparisons of geological material integrating multiple analytical methods and provenance estimations (see 11.4) are ...
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1.1 This guide covers techniques and procedures for the use of powder X-ray diffraction (XRD) in the forensic analysis of geological materials (to include soils, rocks, sediments, and materials derived from them such as concrete), to enable non-consumptive identification of solid crystalline materials present as single components or multi-component mixtures.  
1.2 This guide makes recommendations for the preparation of geological materials for powder XRD analysis with adaptations for samples of limited quantity, instrumental configuration to generate high-quality XRD data, identification of crystalline materials by comparison to published diffraction data, and forensic comparison of XRD patterns from two or more samples of geological materials to support criminal investigations.  
1.3 Units—The values stated in SI units are to be regarded as standard. Other units are avoided, in general, but there is a long-standing tradition of expressing X-ray wavelengths and lattice spacing in units of Ångströms (Å). One Ångström = 10–10 meter (m) = 0.1 nanometer (nm).  
1.4 This standard is intended for use by competent forensic science practitioners with the requisite formal education, discipline-specific training (see Practice E2917), and demonstrated proficiency to perform forensic casework.  
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
4.1 The design and configuration of an audio laboratory, as well as the maintenance of equipment, are factors that must be considered to ensure an optimal environment to produce the best results. This guide is intended to provide general guidance for laboratory setup and maintenance.  
4.2 This document is not meant to be an all-inclusive guide on how to set up a laboratory; nor does it contain information pertaining to specific commercial products as it relates to computer hardware, forensic, and non-forensic software applications.  
4.3 When dealing with equipment and technology outside your area of expertise, consult with an appropriate specialist.
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1.1 This guide sets forth recommendations for the creation of a forensic audio laboratory space as well as the configuration, verification, and maintenance of the equipment contained within the lab.  
1.2 In designing and configuring an audio laboratory, it is important to consider the acoustical environment/room of the laboratory, as well as climate control. Other than having a viable location for the laboratory, computer hardware and software applications are the most important components of a laboratory.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
4.1 A training program provides the theoretical foundation and practical skills necessary for a trainee to become a qualified forensic hair examiner.  
4.2 A trainee is directly supervised by a qualified examiner throughout their training. At the end of the training program, a successful trainee is capable of forming opinions, presenting and explaining evidence, and understanding the limitations of analytical results and interpretations based upon sound scientific knowledge, validated procedures, and practical experience.  
4.3 A trainee is required to meet the minimum training criteria in Practice E2917 and this practice. It is the laboratory management’s responsibility to ensure the selected trainee has the appropriate educational background. It is recommended that, at a minimum, the trainee possess a bachelor’s degree in a natural science.  
4.4 This document outlines lessons, practical exercises, and criteria to monitor and evaluate trainee progress and is designed to be incorporated into an overall laboratory training program.  
4.5 Additional training beyond that which is listed here should be made available to the trainee. Such training might include off-site courses, internships, and specialized training by experienced examiners. Continuing education and training will provide a forensic examiner the opportunity to remain current in the field.  
4.6 Additional analyses can be performed on hairs that have been chemically altered (for example, dyed hair) or have trace materials on the surface (for example, glitter). Such techniques are beyond the scope of this document.  
4.7 This practice addresses the benefit of following microscopical examinations with DNA analysis.
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1.1 This practice covers training guidelines for use by forensic laboratory personnel responsible for training forensic examiners that will perform hair examinations, including microscopical comparisons of human hair.  
1.2 Forensic hair examiners are trained in accordance with Practice E2917 and the discipline-specific guidelines (Guide E3316) and criteria within this practice.  
1.3 This practice contains guidelines that include the tasks, goals, and objectives that allow the trainee to acquire the requisite knowledge, skills, and abilities to independently perform casework in the microscopical examination of hair.  
1.4 This practice addresses the correlation between the analytical results of microscopical examinations and the potential for DNA analysis.  
1.5 This practice addresses training for the microscopical examination of hairs and not the examination of chemical alterations (for example, hair dye) or trace materials on the surface of hairs (for example, hair care products).  
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
5.1 This practice is intended to be used by subject matter experts in the field of forensic pGSR analysis who have met their laboratory’s technical requirements to be assigned to the role of trainer in the category of testing that deals with the detection, analysis, and classification of pGSR particles.  
5.2 This practice is intended to be used in conjunction with Practice E2917, as well as the laboratory’s existing generalized training protocols, standard operating procedures, and quality practices, to develop a complete training-to-competency program in pGSR analysis by SEM/EDS. This practice provides the required additional, discipline-specific elements for pGSR analysis by SEM/EDS, in accordance with 5.3.2 of Practice E2917; it does not include the core specific elements covered in 5.3.1 of Practice E2917.  
5.3 The topics and procedures outlined in this practice are grounded in the body of scientific literature that exists in the field of pGSR examination.  
5.3.1 Additional sources of information on pGSR examination, not specifically mentioned in this document, should be considered, added, or substituted. A review of new sources of information on general forensic methods and pGSR examinations should be carried out on a regular basis (e.g. annually or biannually) to incorporate well-established current findings and methods into the training program and to replace any outdated methods.  
5.3.2 When possible, make additional training available to the trainee. Such training might include off-site short courses, short internships, and specialized training by experienced examiners.
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1.1 This practice describes the minimum requirements of a training program in primer gunshot residue (pGSR) analysis by scanning electron microscopy/energy dispersive X-ray spectroscopy (SEM/EDS). It describes lessons, practical exercises, and progress monitoring and evaluation that should be part of a laboratory’s training program.  
1.2 The primary purpose of this practice is to facilitate the development and implementation of training programs in crime laboratories or other such analytical entities that participate in the detection, analysis, and classification of pGSR particles.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

ABSTRACT
These specifications describe the basic elements of a request for the scientific examination of physical evidence collected in the investigation of a sexual assault. These specifications outline considerations that will facilitate the analysis of sexual assault evidence by a potentially large group of forensic experts. The request for scientific examination of sexual assault evidence should include a completed sexual assault medical/nurse sexual assault examiner/medical examiner evaluation form in accordance with the requirements.
SIGNIFICANCE AND USE
5.1 These specifications are designed to ensure that evidence associated with a sexual violence investigation is handled and analyzed in the most useful manner and without wasting evidentiary materials and analytical time or resources, or both.
SCOPE
1.1 These specifications describe the basic elements of a request for the scientific examination of physical evidence collected in the investigation of a sexual violence incident. These specifications are designed to be used in conjunction with other specifications, guides, and practices associated with sexual violence examinations that are listed in Section 2.  
1.2 These specifications outline considerations that facilitate the analysis of sexual violence evidence by forensic experts. These experts can include, but are not limited to, serologists, toxicologists, pathologists, odontologists, latent print examiners, firearm and toolmark examiners, and trace materials analysts. The success of their combined work requires a clear understanding of the issues and relationships involved in the case.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
2.1 This practice is intended for use by any technical investigator when investigating an incident that can be reasonably expected to be the subject of litigation. The intent is to obtain sufficient information and physical items to identify evidence associated with the incident and to preserve it for analysis.  
2.2 The quality of evidence may change with time, therefore, special effort should be taken to capture and preserve evidence in an expeditious manner. This practice sets forth guidelines for the collection and preservation of evidence for further analysis.  
2.3 Evidence that has been collected and preserved is identified with, and traceable to, the incident. This practice sets forth guidelines for such procedures.
SCOPE
1.1 This practice covers guidelines for the collection and preservation of information and physical items by any technical investigator pertaining to an incident that can be reasonably expected to be the subject of litigation.  
1.2 This practice describes generally accepted professional principles and operations, although the facts and issues of each situation require consideration, and frequently involve matters not expressly dealt with herein. Deviations from this practice should be based on specific articulable circumstances.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
4.1 This guide describes good practices for the collection (5), packaging, and preservation (8.3) of soils in criminal forensic investigations. Some of the information in this guide is demonstrated in its companion video, which is available on-line and is intended as a complementary resource to this guide (6).  
4.2 Individual agencies can use this guide to develop agency-specific procedures regarding the collection of soils for forensic applications.
SCOPE
1.1 This standard provides guidance to instruct crime scene professionals in good practices for the documentation, collection, and preservation of soil and other geological evidence for use in criminal investigations. Sampling for environmental geology is outside of its scope. It is designed as a resource for professionals whose job responsibilities include the collection and preservation of soil evidence and for forensic scientists to enable them to advise crime scene investigators.  
1.2 The values stated in SI units are to be regarded as standard. The values given in parentheses after SI units are provided for information only and are not considered standard.  
1.3 This standard is intended for use by competent forensic science practitioners with the requisite formal education, discipline-specific training (see Practice E2917), and demonstrated proficiency to perform forensic casework.  
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
4.1 µ-XRF is a nondestructive qualitative elemental analysis technique used for polymers. It involves excitation of a sample by an X-ray source resulting in the emission of characteristic X-rays detected using an energy dispersive X-ray detector. Results are displayed simultaneously as a spectrum of intensity as a function of energy for elements of atomic number 11 or greater.  
4.2 µ-XRF enables the determination of the elemental composition of a specimen and can be utilized for comparisons of components of polymeric materials (for example, tape backings, tape adhesives, paint layers).  
4.3 Comparisons of X-ray spectra acquired from polymer samples are conducted for source discrimination or potential association.  
4.4 Quantitative processes for µ-XRF analysis are available but are not used for polymer analyses because of the lack of prepared polymer standard reference samples.  
4.5 In general, information available from a heterogeneous specimen diminishes as its size is reduced or its condition degrades, which lessens its likelihood of being representative of the source material.  
4.6 µ-XRF data collected from polymers is limited to specific information (for example, elements detected, relative elemental abundance); additional analytical procedures are required to further characterize and identify the chemical composition of the polymer sample.  
4.7 Limitations of µ-XRF include the inability to detect some elements in trace concentrations, the inability to analyze individual particles, the potential interference related to the penetration depth of the beam relative to the sample thickness, the inability to resolve the peaks of some elements (for example, Ba Lα / Ti Kα), and the potential for discoloration of some materials due to exposure to radiation.
SCOPE
1.1 This guide covers recommended techniques and procedures intended for use by forensic laboratory personnel that perform µ-XRF analysis of polymer samples.  
1.2 This guide describes various techniques and procedures used in the µ-XRF analysis of polymers that include sample handling and preparation, instrument operating conditions, and spectral data collection, evaluation and interpretation.  
1.3 This guide describes the application of µ-XRF systems equipped with either mono- or poly- capillary optics and an energy dispersive X-ray detector (EDS).  
1.4 This guide is intended to be applied within the scope of a broader analytical scheme (for example, Guide E1610, Guide E3260) for the forensic analysis of a polymer sample (1-6).2 A µ-XRF analysis can provide additional information regarding the potential relationships between the sources of polymeric materials.  
1.5 The fundamental aspects of the composition and manufacture of polymeric materials or theory of X-ray fluorescence can be found in various texts (7-18).  
1.6 This standard is intended for use by competent forensic science practitioners with the requisite formal education, discipline-specific training (see Practices E2917, E3233, E3234), and demonstrated proficiency to perform forensic casework.  
1.7 Units—The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.8 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.9 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
4.1 This practice is useful for preparing extracts from fire debris for subsequent analysis by gas chromatography-mass spectrometry (see Test Method E1618).  
4.2 This practice is useful to reduce potential fractionation during separation, such as when attempting to distinguish between various grades of fuel oil.  
4.3 This practice is particularly useful for extraction from nonporous surfaces such as glass, or the interior of burned containers. It is also well suited to the extraction of ignitable liquid residues from samples that are not amenable to extraction using Practice E1412.  
4.4 This practice lacks specificity to separate and isolate ignitable liquids from interfering compounds present in the fire debris.  
4.5 This practice is not suitable for the extraction of extremely volatile compounds and ignitable liquids (for example, acetone, butane, ethanol, propane, some cigarette lighter fluids), which could evaporate during the concentration step.  
4.6 This is a destructive technique. Whenever possible, this technique should only be used when a representative portion of the sample can be preserved for reanalysis. Those portions of the sample subjected to this procedure could be unsuitable for resampling. If sample spoliation is an issue, a nondestructive extraction technique (for example, Practices E1412, E2154) should be used prior to this technique.
SCOPE
1.1 This practice covers the procedure for removing small quantities of ignitable liquid residue from samples of fire debris using solvent to extract the residue.  
1.2 This practice is suitable for extracting ignitable liquid residues over a wide range of concentrations.  
1.3 Alternate separation and concentration procedures are listed in the referenced documents (Practices E1388, E1412, E1413, E2154, and E3189).  
1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
3.1 These terms have particular application to fire debris analysis. In addition, several sources of definitions were used in the development of this terminology: Hawley’s Condensed Chemical Dictionary, Fifteenth Edition (1);4  Kirk’s Fire Investigation, Fifth Edition (2); The Chemistry and Technology of Petroleum, Third Edition (3); Merriam-Webster’s Collegiate Dictionary, Tenth Edition (4); and Fire Debris Analysis  (5). A suitable definition was developed after all of the sources were found wanting.
SCOPE
1.1 This terminology standard is a compilation of terms and corresponding definitions that are used in fire debris analysis. Some legal or scientific terms that are generally understood or defined adequately in other readily available sources are included.  
1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.3 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
5.1 TLC is an inexpensive and simple technique that could be used to complement other analytical techniques within a general analytical scheme related to forensic fiber examination.  
5.2 Consider the forensic analysis of fiber colorants using TLC for single fiber comparisons only when the sample size is adequate (that is, enough colorant can be extracted for analysis) and it is not possible to discriminate between the fibers of interest using other techniques, such as comparison microscopy and MSP. Larger fibrous units (for example, thread or tuft) can be treated as an individual sample if determined to be homogeneous. Do not treat fibers that cannot be directly related to each other as a collective sample for the purposes of TLC.  
5.3 The extraction procedures carried out prior to TLC analysis can provide useful information about dye classification. TLC can provide qualitative information about dye components. Similar colors made up of different dye components can be differentiated using this technique. The application of TLC may serve to discriminate between fibers or it may support the possibility of fibers sharing a common source.  
5.4 TLC can be prohibitively difficult or undesirable in some circumstances. Short lengths of fibers or pale-colored fibers can lack adequate amounts of colorant necessary to be examined by TLC. Dye extraction from some fibers can be impossible (2, 3). Some fiber types do not truly extract, but change or lose color. Reactive dyes are covalently bonded to the fiber and typically cannot be removed by conventional extraction methods, but can be released from cotton and wool by disrupting the fiber by enzymatic or chemical digestion, respectively (1). The desire to preserve evidence from deleterious change or for possible analysis by another examiner can preclude removing the color or employing a destructive method for analysis.
SCOPE
1.1 This guide is intended as an overview of the Thin-Layer Chromatography (TLC) of fiber colorants (or individual dye components) present in dyed fibers. It is intended to be applied within the scope of a broader analytical scheme for the forensic analysis of fiber samples. TLC could provide information that cannot be obtained through other color analyses (such as microspectrophotometry (MSP)) (1).2  
1.2 This standard is intended for use by competent forensic science practitioners with the requisite formal education, discipline-specific training (see Practice E2917), and demonstrated proficiency to perform forensic casework (see Practice E3255).  
1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
4.1 Microscopical examination is generally a non-destructive, rapid, and reproducible means of determining the microscopic characteristics, optical properties, and generic polymer type of textile fibers.  
4.2 Side-by-side microscopical comparisons provide a highly discriminating and efficient method of determining if two or more fibers can be differentiated.  
4.3 This guideline requires specific pieces of instrumentation outlined herein.
SCOPE
1.1 This standard covers guidelines for microscopical examinations employed in forensic fiber classification, identification, and comparison. The microscopical examination of fibers includes the use of a variety of light microscopes, such as stereomicroscopes, compound microscopes, and comparison microscopes, as well as a variety of illumination types, such as bright field, polarized light, fluorescence, and interference. In certain instances, the scanning electron microscope can yield additional information. The particular test(s) or techniques employed by each examiner or laboratory will depend upon available equipment, examiner training, and the nature and extent of the fiber evidence.  
1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.3 This standard is intended for use by competent forensic science practitioners with the requisite formal education, discipline-specific training (see Practice E2917), and demonstrated proficiency to perform forensic casework.  
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
4.1 Color is an easily observable characteristic of soils and is integral to the taxonomic classifications of soils (6-8); factors including parent material, hydrology, vegetation, and extent of soil weathering, can affect soil color, making color a valuable diagnostic tool for forensic examination purposes.  
4.2 Soil color is sufficiently variable among soils to be used for differentiation of many soils in forensic examinations (9, 10) (see Section 6 for the test method for color determination and comparison criteria) as determined by visual characterization in the Munsell color system.  
4.3 Instrumental techniques are suitable for color determination of soil evidence but are not covered within this practice.  
4.4 Color determinations of soil samples are also used within soil provenance assessments to provide investigative leads or aid in searches. Interpretation of soil color for soil provenance is case-specific and beyond the scope of this practice, but the methods of color determination (6.5.1 to 6.5.2) and documentation (6.7) described here should be applied to soil color within soil provenance cases.
SCOPE
1.1 This practice covers visual color determination of soil/geologic material within the context of a forensic examination and is intended for use by laboratory personnel.  
1.1.1 This practice recommends use of soil color for: the initial screening of soil samples in forensic casework, prioritization of known soil exemplars for detailed analysis, and includes a test method for color determination in the Munsell color system and comparison among samples.  
1.2 Units—Units in the Munsell color system are used throughout this document.  
1.3 This practice is intended for use by competent forensic science practitioners with the requisite formal education, discipline-specific training (see Practice E2917), and demonstrated proficiency to perform forensic casework.  
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
5.1 This guide is designed to assist an examiner in the selection of appropriate sample preparation methods for the analysis, comparison, and identification of fibers using IR spectroscopy. IR spectroscopy can provide additional compositional information than is obtained using polarized light microscopy alone. The extent to which IR spectral comparison is conducted will vary with specific sample and case evaluations.  
5.2 IR analysis should follow visible and fluorescence comparison microscopy, polarized light microscopy, and ultraviolet (UV)/visible spectroscopy. If no exclusionary differences are noted between the known and unknown samples in optical properties, then proceed to IR spectroscopy as the next step in the analytical scheme, as applicable.  
Note 1: IR analysis generally follows the aforementioned techniques since sample preparation (for example, flattening) irreversibly changes fiber morphology.  
5.3 IR spectroscopy should be conducted before dye extraction for chromatography due to the semi-destructive nature of the extraction technique. Because of the large number of sub-generic classes, forensic examination of acrylic and modacrylic fibers is likely to benefit significantly from IR spectral analysis (5). Useful distinctions between subtypes of nylon and polyester fibers can also be made by IR spectroscopy.  
5.4 IR spectroscopy can provide molecular information regarding major organic and inorganic components. Components in lesser amounts are typically more difficult to identify. Reasons for this include interference of the absorption bands of the major components with the less-intense bands of minor components, and sensitivity issues whereby the minor components are present at concentrations below the detection limits of the instrument.  
5.5 Fiber samples are prepared and mounted for microscopical IR analysis by a variety of techniques. IR spectra of fibers are obtained using an IR spectrometer coupled with an IR microscope, ATR, or diamond...
SCOPE
1.1 Infrared (IR) spectroscopy is a valuable method of fiber polymer identification and comparison in forensic examinations. The use of IR microscopes, coupled with Fourier transform infrared (FTIR) spectrometers, has greatly simplified the IR analysis of single fibers, thus making the technique feasible for routine use in the forensic laboratory. This guide provides basic recommendations and information about IR spectrometers and accessories, with an emphasis on sampling techniques specific to fiber examinations. The particular method(s) employed by each examiner or laboratory will depend upon available equipment, examiner training, sample suitability, and sample size.  
1.2 This guide is intended for examiners with a basic knowledge of the theory and practice of IR spectroscopy, as well as experience in the handling and forensic examination of fibers. In addition, this guide is to be used in conjunction with a broader analytical scheme.  
1.3 If polymer identification is not readily apparent from optical data alone, an additional method of analysis, such as microchemical tests, melting point, IR spectroscopy, Raman spectroscopy, or pyrolysis gas chromatography, should be used. An advantage of IR spectroscopy is that the instrumentation is readily available in most forensic laboratories and the technique is minimally destructive.  
1.4 Units—The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.5 This standard is intended for use by competent forensic science practitioners with the requisite formal education, discipline-specific training (see Practice E2917), and demonstrated proficiency to perform forensic casework.  
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practice...

SIGNIFICANCE AND USE
5.1 The construction, composition, and color of textiles contain useful comparative characteristics for forensic examinations. Textiles are observed in a variety of constructions: woven, knit, nonwoven, or in combination. The range of colors in which textiles are offered in the marketplace is vast and constantly changing due to styles and seasons.  
5.2 A complete characterization of the fabrics, including their construction, and other materials used in the assemblage of a textile (for example, sewing thread) is a critical component of a comprehensive forensic fabric or cordage examination.
SCOPE
1.1 This guide is intended to assist individuals and laboratories that conduct examinations of fabrics and cordage for the purposes of analyzing and comparing types of fabric, cordage and damage. A complete characterization of the fabrics, including their construction and other materials used in the assemblage of a textile (for example, sewing thread), is a critical component of a comprehensive forensic fabric or cordage examination.  
1.2 The values stated in inch-pound units are to be regarded as standard. No other units of measurement are included in this standard.  
1.3 This standard is intended for use by competent forensic science practitioners with the requisite formal education, discipline-specific training (see Practice E2917), and demonstrated proficiency to perform forensic case work  
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
5.1 The protocols that exist for photographing a decedent’s face at autopsy for identification purposes (for example, NAME Forensic Autopsy Performance Standards) do not always result in the capture of facial images that can be used for automated FR searches or manual facial comparisons. It is not always feasible to collect fingerprints from decedents (for example, in disaster situations or when a decedent is in a state of advanced decomposition), and radiograph (medical or dental) comparison requires at least a presumptive identification of remains so appropriate comparative antemortem radiographs can be obtained to confirm the identification. If the decedent’s DNA or appropriate family reference DNA profiles are not already stored within a DNA repository (for example, the FBI’s CODIS), a DNA association will also require the presumptive identification of a decedent to ensure that appropriate samples are collected for comparison/association.  
5.2 It is advisable to follow the guidelines presented in this guide even when not all facial components are present as even incomplete facial images can assist automated FR and manual facial comparison processes, especially through more accurate recording of minute facial details.  
5.3 For the purpose of facial image capture, there are various perimortem or postmortem conditions or both that can degrade the usability of any facial images captured:  
5.3.1 Presence of trauma (for example, entry/exit wounds, lacerations, bruising, missing components, etc.),  
5.3.2 Obscuring matter (for example, blood, fluids, dirt, debris, hair, clothing accessories, and so forth), and  
5.3.3 Decomposition and other postmortem changes (for example, bloating, mummification, skeletonization, evidence of insect or scavenger activity, etc.).  
5.4 Before any attempt is made to clean or alter the decedent for facial image capture, nationally accepted standards or agency protocols or both should be followed so the alterations do not affect ...
SCOPE
1.1 The purpose of this document is to provide guidelines for capturing postmortem facial images of human remains in controlled (for example, morgue) and semi-controlled (for example, field) settings to facilitate automated facial recognition (FR) searches or manual facial comparisons that could contribute to forensic investigations.  
1.2 The values stated in SI units are to be regarded as standard. The values given in parentheses are mathematical conversions to inch-pound units that are provided for information only and are not considered standard.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
4.1 The key factors that determine image quality for highly controlled facial images (for example: passports, police mugshots, drive motor vehicle, etc.) are well understood with respect to their use in automated FR systems and are a critical factor which directly affects the searching accuracy of the FR system.  
4.2 Image quality also plays a major role in determining the extent to which a trained facial examiner is able to reach a conclusion as to whether two images containing faces are likely to be of the same person or not. This applies to either reviewing a FR system candidate result set or 1:1 image comparisons.  
4.3 This guide provides guidance for the capture of facial images under controlled, semi-controlled, and uncontrolled scenarios for constraints which can be categorized into the following types of requirements:  
4.3.1 Scene, refers to the content, subject and background in the image,  
4.3.2 Photographic, refers to lighting, focus and other constraints required for image capture, and  
4.3.3 Digital, refers to the conversion of the captured image into a digital record.
SCOPE
1.1 This guide is intended for use by practitioners who are choosing, setting up, and operating photographic equipment designed to capture facial images for use with an automated Facial Recognition System or used for manual comparisons by a trained facial examiner. This guide provides an overview of how to achieve the specifications defined in Annex E of ANSI/NIST-ITL-1-2011, Update 2015, for capturing facial images.  
1.2 Annex E of ANSI/NIST-ITL-1-2011 defines a well-controlled capture environment and subject whereas this document will give guidance where tight controls in the capture environment and subject control cannot be achieved.  
1.3 This guide addresses equipment considerations for two-dimensional (2D) conventional images. It does not address video, scanners, or three-dimensional (3D) capture.  
1.4 The values stated in SI units are to be regarded as standard. The values given in parentheses are mathematical conversions to non-SI units that are provided for information only and are not considered standard.  
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
4.1 This practice establishes procedures to be followed by the forensic science practitioner to document the nature, state, or condition of items of evidence. It also describes specific actions that are required for destructive testing if planned testing, examination, disassembly, or other actions are likely to alter the nature, state, or condition of the evidence so as to preclude or adversely limit additional examination or testing.  
4.2 Deviations from this practice are not necessarily wrong or inferior, but such deviations should be justified and documented.
SCOPE
1.1 This practice covers procedures for the examination and testing of evidence items or systems that may have been involved in a specific incident which are, or may be reasonably expected to be, the subject of civil or criminal litigation.  
1.2 This practice is applicable when it is determined that examination or testing of evidence is required, and such examination is likely to change the nature, state, or condition of the evidence.  
1.3 This practice is intended for use by competent forensic science practitioners with the requisite formal education, discipline-specific training (see Practice E2917), and demonstrated proficiency to perform forensic casework.  
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
4.1 A microscopical hair examination is conducted to determine if the item is a hair; from a human; from a particular somatic region; characteristic of a broad geographically-assigned ancestral group; characteristic of a particular growth phase; damaged; symptomatic of disease, condition, or disorder; forcibly removed; chemically altered (for example, dyed or bleached); suitable for microscopical comparison; suitable for DNA analysis; and similar to or different from a known sample (4-9).  
4.2 Most often, hairs from the head and pubic regions of the body are used for microscopical comparisons. There is usually more interpersonal variability in the characteristics of head and pubic hairs than in the hairs from other somatic regions. Head hairs usually show more interpersonal variation than pubic hairs. Hairs from other somatic regions may also be compared, but these comparisons are usually limited and less frequently conducted. Accordingly, this guide primarily considers human head and pubic hair comparisons.  
4.3 Microscopical hair comparisons are not a means of individualization (10). This limitation is to be stated in any communication (for example, reports, testimony) when an association is reported.  
4.4 Additional analyses can be performed on hairs that have been chemically altered (for example, dyed hair) or have trace materials on the surface (for example, glitter). Such techniques are beyond the scope of this document.
SCOPE
1.1 This guide covers procedures used by forensic laboratory personnel in the forensic examination of hair by microscopy, including microscopical comparisons and classification of hair samples.  
1.2 This guide addresses instrument setup, hair collection, sample handling techniques, and the use of various microscopes in the examination and comparison of hair.  
1.3 This guide addresses the benefit of following microscopical examinations with DNA analysis.  
1.4 This standard is intended for use by competent forensic science practitioners with the requisite formal education, discipline-specific training (see Practice E2917), and demonstrated proficiency to perform forensic casework.  
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
4.1 This guide provides guidance in the selection of appropriate sample preparation methods and instrumental parameters for the analysis, comparison, or identification of various polymeric materials by PGC and PGC/MS.  
4.1.1 PGC/MS can differentiate between classes of fibers (for example, acrylic, polyester, nylon) and within classes of fibers (for example, acrylics) (1-3).5  
4.1.2 Paint binders are differentiated based upon the variety of monomers used in paint formulations which could be difficult to identify by other analytical techniques. In addition, some additives can be detected or identified.  
4.1.3 Differentiation can be achieved by the separation and identification of organic components in the adhesive portion of tapes (4, 5) and in the backings of electrical tapes (6).  
4.1.4 PGC/MS can provide additional discrimination for other types of polymers such as automotive lenses, automotive body fillers, cosmetics, plastics, and rubbers (7-9).  
4.2 Pyrolysis breaks a large molecule into many smaller molecules in a reproducible fashion through the breaking of bonds by means of the application of thermal energy. Analytical pyrolysis is used to provide chemical information on organic-containing solids that cannot be dissolved or otherwise introduced into a chromatographic system. It is also used to analyze and compare solvents bound in a solid material (such as tape adhesives) (10). When analyzed using a separation technique such as gas chromatography, the smaller molecules produced through the action of pyrolysis form a pattern of separated fragments. Mass and structural information indicative of the original molecule are also available when a mass spectral detector is used.  
4.3 Although a destructive method, and therefore often placed at the end of an analytical scheme, the pyrograms produced from different polymer compositions form characteristic patterns that are useful for both identification of polymer type and comparisons between samples (4, 6...
SCOPE
1.1 This guide covers information and recommendations for the selection and application of various PGC and PGC/MS procedures and methods in the forensic examination of polymeric materials (for example, fibers, paint, tape). PGC and PGC/MS methods are used for the identification and comparison of the organic components of these materials. Refer to Practice D3452 for further information on the preparation of the pyrolysis system for polymeric analyses.  
1.2 This guide is to be used in conjunction with a broader analytical scheme such as Guides E1610 or E3260, or the SWGMAT Forensic Fiber Examination Guidelines.  
1.3 This standard is intended for use by competent forensic science practitioners with the requisite formal education, discipline-specific training (see Practices E2917, E3233, E3234), and demonstrated proficiency to perform forensic casework.  
1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
4.1 This guide is intended to advise and assist the analyst in the preparation of polymer samples (for example, paint and tape) for SEM/EDS, the collection of data by SEM/EDS, and the interpretation of images and data resulting from these analyses.  
4.2 When polymers are constructed as layered materials, SEM/EDS analysis is conducted on each polymeric layer individually. This analysis can be hindered by a non-discernable layer structure (for example, smear, irregular segregation within the layer system).  
4.3 SEM-EDS data can be useful in:  
4.3.1 Layer Elucidation—SEM images provide insight into the layer structure of a sample.  
4.3.2 Texture Elucidation—SEM images and elemental maps provide insight into the texture (for example, surface topography, distribution of inclusions).  
4.3.3 Element Identification—Determination of the elements detected in a sample layer.  
4.3.4 Relative Elemental Abundance Determination—An EDS spectrum permits the relative abundance of elements in samples to be compared.  
4.4 In the context of a forensic polymer comparison, the evaluation of SEM/EDS results are intended to provide insight into the following forensic tasks:  
4.4.1 Comparison of structure, texture, and elemental data.  
4.4.2 Support for results from other instruments (for example, the presence of calcium, oxygen, and carbon in the EDS spectrum obtained from discrete particles indicates the presence of calcium carbonate as observed in an infrared spectrum). Refer to Guides E2937 and E3085 for further details.  
4.4.3 Significance of results given the presence of certain elements, layer structures, or textures.
SCOPE
1.1 This guide covers recommended techniques and procedures intended for use by forensic laboratory personnel that perform SEM/EDS analyses on polymer samples.  
1.2 This guide describes various techniques and procedures used in the SEM/EDS analysis of polymers that include sample handling and preparation, instrument operating conditions, and spectral data collection, evaluation and interpretation.  
1.3 The theoretical aspects of many of the topics presented can be found in texts such as Scanning Electron Microscopy and X-ray Microanalysis (1).2  
1.4 This guide is intended to be applied within the scope of a broader analytical scheme (for example, Guides E1610, E3260) for the forensic analysis of a polymer sample. An SEM/EDS analysis can provide additional information regarding the potential relationships between the sources of polymeric materials.  
1.5 This guide is intended for use by competent forensic science practitioners with the requisite formal education, discipline-specific training (see Practices E2917, E3233, and E3234), and demonstrated proficiency to perform forensic casework.  
1.6 The values stated in SI units are to be regarded as standard. Other units of measurement are included in this standard where applicable as a result of common usage (for example, keV).  
1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.8 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
5.1 This practice is designed to assist the forensic explosives examiner in selecting and organizing an analytical scheme for identifying intact explosives. The amount and condition of the sample, as well as the availability of applicable instrumental techniques, will determine the selected analytical scheme.  
5.2 The forensic explosives examiner considers relevant issues about the case investigation and submitted items, such as sample size, complexity and condition, environmental effects, and collection methods used. Considerations include test methods, sample preparation schemes, test sequences, and acceptable degrees of sample alteration and consumption that will be different for each case submission.  
5.3 This practice provides the minimum criteria for identifying explosive material. The examiner determines an analytical scheme that uses techniques to correctly identify the material, which could include oxidizers, fuels, binders, and detection agents.  
5.4 This practice is used in conjunction with the referenced documents.  
5.5 This practice does not attempt to address all the issues regarding sample analyses. There could be additional tests or analyses performed to provide further discrimination and characterization of samples.
SCOPE
1.1 This practice covers the evaluation, selection, and application of techniques to establish examination schemes for use by forensic explosives examiners to identify intact (unexploded) low and high explosives. A foundation for a consistent approach to the analysis of intact explosives is provided. Methods for the development of identifying information that follows an efficient order of testing are described.  
1.2 This practice establishes requirements for the use of visual, physical, analytical, and instrumental techniques that provide structural and chemical information for an identification of a high or low explosive material.  
1.3 Techniques used in the examination of explosives include visual and microscopical inspection, physical characterization, ignition susceptibility testing, chemical and spot testing, and instrumental methods.  
1.4 The minimum requirements for identification of commonly encountered explosives and explosive materials are listed.  
1.5 This standard is intended for use by competent forensic science practitioners with the requisite formal education, discipline-specific training (see Practice E2917), and demonstrated proficiency to perform forensic casework (refer to the T/SWGFEX Suggested Guide for Explosives Analysis Training).  
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
6.1 This practice is designed to assist the forensic explosives examiner in selecting and organizing an analytical scheme for identifying explosive residues. The amount and condition of the sample, as well as the availability of instrumental techniques, will determine the selected analytical scheme.  
6.2 The forensic explosives examiner considers relevant issues about the case investigation and submitted items, such as sample size, complexity and condition, environmental effects, and collection methods used. Considerations include test methods, sample preparation schemes, test sequences, and acceptable degrees of sample alteration and consumption that will be different for each case submission.  
6.3 This practice is used when insufficient material is present to follow Practice E3253.  
6.4 This practice is used in conjunction with the referenced documents.  
6.5 This practice does not attempt to address all the issues regarding sample analyses. There could be additional tests or analyses performed to provide further discrimination and characterization of samples.
SCOPE
1.1 This practice covers the evaluation, selection, and application of techniques to establish an examination scheme for use by forensic explosives examiners to identify residues from low and high explosives. A foundation for the consistent approach to the analysis of visible and non-visible explosive residues from post-blast or other explosive-related scenes is provided. Methods for the development of identifying information that follows an efficient order of testing are described.  
1.2 This practice establishes requirements for the use of visual, physical, analytical, and instrumental techniques that provide structural and chemical information for an identification of an explosive residue.  
1.3 Techniques used in the examination of explosive residues include visual and microscopical inspection, physical characterization, ignition susceptibility testing, chemical and spot testing, and instrumental methods.  
1.4 This standard is intended for use by competent forensic science practitioners with the requisite formal education, discipline-specific training (see Practice E2917), and demonstrated proficiency to perform forensic casework (refer to the T/SWGFEX Suggested Guide for Explosives Analysis Training).  
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
5.1 Processed images are used for many purposes by the forensic science community. They can yield information not readily apparent in the original image, which can assist an expert in drawing a conclusion that might not otherwise be reached.  
5.2 This guide addresses image processing and related legal considerations in the following three categories:  
5.2.1 Image enhancement,  
5.2.2 Image restoration, and  
5.2.3 Image compression.
SCOPE
1.1 This guide provides digital image processing guidelines to ensure the production of quality forensic imagery for use as evidence in a court of law.  
1.2 This guide briefly describes advantages, disadvantages, and potential limitations of each major process.  
1.3 This standard cannot replace knowledge, skills, or abilities acquired through education, training, and experience, and is to be used in conjunction with professional judgment by individuals with such discipline-specific knowledge, skills, and abilities.  
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
3.1 These terms have particular application to explosives analysis. In addition, several sources of definitions were used in the development of this terminology: Hawley’s Condensed Chemical Dictionary, Sixteenth Edition  (1);3  Practical Bomb Scene Investigation, Third Edition  (2); Forensic Investigation of Explosions  (3); Chemistry of Pyrotechnics, Third Edition  (4); Explosives, Seventh Completely Revised and Updated Edition  (5); Dictionary of Explosions & Explosives  (6); PICATINNY Encyclopedia of Explosives and Related Items  (7, 8); Merriam-Webster.com Dictionary  (9); and DOD Dictionary of Military and Associated Terms  (10). A suitable definition was developed after all of the sources were found wanting.
SCOPE
1.1 This is a compilation of terms and corresponding definitions related to the analysis of explosives. Legal or scientific terms that are generally understood or defined adequately in other readily available sources may not be included.  
1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.3 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
4.1 This guide is designed to be used by forensic service providers when issuing final reports on pGSR analyses by SEM/EDS.  
4.2 This guide is intended to be used in conjunction with Practice E1588, Practice E620, and the SWGGSR Guide.
SCOPE
1.1 This guide describes the contents of a formal, written technical report expressing the results and interpretation of pGSR particle analysis by SEM/EDS by forensic service providers.  
1.2 This guide is intended for use by competent forensic science practitioners with the requisite formal education, discipline-specific training (see Practices E2917), and demonstrated proficiency to perform forensic casework.  
1.3 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
5.1 The preservation and storage of extracts recovered from fire debris or liquids submitted in a fire investigation provides a mechanism for reanalysis in the event that the original evidence is altered due to factors such as the extraction process (Practices E1386 and E1413), sample degradation, or failure of the original evidence container during post-analysis storage.  
5.2 Reanalysis of a stored sample extract could result in data that do not duplicate the data obtained during the initial analysis. Loss of the more volatile components of a stored sample can occur, and this possibility should be considered when interpreting data from a stored sample. Studies of gasoline and diesel show that results (that is, determination of presence and classification of an ignitable liquid, or determination of absence of an ignitable liquid) obtained from reanalysis data are in agreement with the initial analytical results. (1-4)  
5.3 Preserved extracts are either returned to the submitter for storage or catalogued and stored by the laboratory or other designee.
SCOPE
1.1 This standard covers procedures for the preservation of ignitable liquids and ignitable liquid residue extracts obtained from fire debris samples and questioned ignitable liquid samples. Extraction procedures are described in Section 2, Referenced Documents.  
1.2 Specific evaluation of this practice is limited to the preservation of gasoline and diesel fuel (1-4),2 the components of which together span the range of chemical classes and volatility of ignitable liquid residues commonly encountered in fire debris samples.  
1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
5.1 This guide is designed to assist an analyst in the selection of appropriate sample preparation methods and instrumental parameters for the analysis and comparison of paint pigments and colors. When used for comparison purposes, the goal is to determine whether any exclusionary differences exist between the samples.  
5.2 Paint sample spectra can be measured by reflectance or transmittance spectroscopy for comparison purposes. Transmittance measurements are generally preferred and are required for the analysis of UV absorbers in clear coats and the detailed analysis of effect pigments that are not opaque. Emission comparison by means of fluorescence is also measurable.  
5.3 It is not the intention of this guide to present comprehensive theories and methods of MSP. It is necessary that the analyst have an understanding of UV-Vis-NIR MSP and general concepts of specimen preparation before using this guide. This information is available from manufacturers’ reference materials, training courses, and references such as Eyring (1),3 Stoecklein (2), and Purcell (3).
SCOPE
1.1 This guide is intended to assist forensic analysts who conduct UV, visible, NIR, or fluorescence emission spectral analyses on small fragments of paint or use Guide E1610, as this guide is to be used in conjunction with a broader analytical scheme.  
1.2 This guide deals primarily with color measurements within the visible spectral range but will also include some details concerning measurements in the UV and NIR spectral ranges. The particular method(s) employed by each analyst depends upon available equipment, examiner training (Practices E2917, E3234), sample suitability, and sample size.  
1.3 This guide provides basic recommendations and information about microspectrophotometers.  
1.4 This guide does not address other areas of color evaluation such as colorimetric values, paint surface texture or pigment particle size, shape, or dispersion within a paint film that are evaluated by other forms of microscopy.  
1.5 This guide is directed at the color analysis of commercially prepared paints and coatings. It does not address the analysis or determination of provenance of artistic, historical, or restorative paints, but it could be useful in those fields.  
1.6 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.7 This standard is intended for use by competent forensic science practitioners with the requisite formal education, discipline-specific training (see Practices E2917, E3234), and demonstrated proficiency to perform forensic casework.  
1.8 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.9 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
5.1 The procedure described in this document is in accordance with current SWGFAST guidelines (6), as well as National Institute of Standards and Technology (NIST) standard (7), which specify 1000 pixels per inch (ppi) at 1:1 as the minimum scanning resolution for latent print evidence. This standard appears primarily to be historical and directed towards scanners, rather than cameras, though recent studies suggest that it is suitable for capturing Level 3 detail (8).  
5.2 While the 1000 ppi resolution standard permits the capture of level three detail in latent prints, it does not mean that any image recorded at a lower resolution would necessarily be of no value for comparison purposes. Such an image could have captured level two details sufficiently for comparison. However, there are some latent print impressions that are so degraded or contain such limited quantity of information that at least 1000 ppi resolution is required to conduct an accurate examination. Some automated fingerprint identification systems require 1000 ppi for submission purposes. The relationship between machine (optical) resolution and achievable resolution (sometimes called resolving power) can vary greatly by manufacturer (8).
SCOPE
1.1 This practice provides recommendations on the resolving power that enables recording of Level 3 details of latent print evidence that are suitable for comparison purposes using a digital camera, a flatbed scanner, or other image capture device. These recommendations take into consideration the minimum resolution requirements for utilizing the photographs for comparison.  
1.2 This practice describes procedures that can be used to verify the resolving power of such imaging systems and recommends equipment to be used.  
1.3 Certain commercial equipment, instruments, or materials are used in this document as representative examples to more clearly explain the procedures. Such use does not imply a recommendation or endorsement.  
1.4 This standard is intended for use by competent forensic science practitioners with the requisite formal education, discipline-specific training (see Practice E2917), and demonstrated proficiency to perform forensic casework.  
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
4.1 These are minimum standards of quality assurance applicable to forensic science service providers performing forensic chemical analysis on evidence.  
4.2 This practice is to be used by forensic science practitioners performing chemical analysis on evidence and reinforced by forensic science service provider management.
SCOPE
1.1 This practice discusses procedures for quality assurance of forensic science service providers performing forensic chemical analysis. This practice provides a framework of quality in the processing of evidence, including: maintaining a quality management system; personnel duties, qualifications, training, and education; facility considerations; evidence handling; analytical procedures; instrument and equipment performance; chemicals and reagents; casework documentation and reporting; proficiency and competency testing; method validation and verification; audits; deficiency of analysis; and documentation requirements. Annex A1 – Annex A3 provide additional procedures that are discipline-specific.  
1.2 This practice cannot replace knowledge, skills, or abilities acquired through appropriate education, training, and experience (see Practice E2917), and is to be used in conjunction with professional judgment by individuals with such discipline-specific knowledge, skills, and abilities.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
6.1 This guide is designed to assist the forensic tape examiner in selecting and organizing an analytical scheme for the analysis, comparison, and identification of tapes. The size and condition of the sample(s) influences the choice of analytical scheme. The evaluation and interpretation of the data for each technique is an important part of an analytical scheme but it is outside the scope of this guide. These will be addressed in other ASTM standards.
SCOPE
1.1 This guide is intended as an introduction to other standard guides for the forensic examination of pressure sensitive adhesive tape. It is intended to assist individuals who conduct forensic tape analyses in their evaluation, selection, and application of tests that can be of value to their examinations. This guide describes the construction and classification of various tapes and the methods to develop discriminatory information using an efficient order of testing. This standard provides an overview and guidance on the strengths and limitations of various techniques used in the analysis and comparison of pressure sensitive adhesive tapes. The goal is to provide a consistent approach to forensic tape analysis.  
1.2 The forensic tape examiner addresses concerns such as sample size, complexity and condition of the sample, environmental effects, collection and packaging methods, and case/investigation specific issues. These factors require that the forensic tape examiner choose test methods, sample preparation schemes, testing sequences, and degree of sample alteration and consumption that are suitable to each specific case.  
1.3 This standard is intended for use by competent forensic science practitioners with the requisite formal education, discipline-specific training (see Practice E2917, Practice E3233), and demonstrated proficiency to perform forensic casework.  
1.4 The values stated in SI units are to be regarded as standard. Other units of measurement are included in this standard as applicable to industrial usage.  
1.5 Some of the methods discussed in this guide involve the use of chemicals, temperatures, and radiation sources. This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
5.1 This document will be of use to forensic laboratory personnel who are involved in the analysis of GSR samples by SEM/EDS (5).  
5.2 SEM/EDS analysis of GSR is a non-destructive method that provides (6, 7) both morphological information and the constituent elements detected in individual particles.  
5.3 Particle analysis contrasts with bulk sample methods, such as atomic absorption spectrophotometry (AAS) (8), neutron activation analysis (NAA) (9), inductively coupled plasma atomic emission spectrometry (ICP-AES), and inductively coupled plasma mass spectrometry (ICP-MS), where the sampled material is dissolved or extracted prior to the determination of total element concentrations, thereby sacrificing size, shape, and individual particle identification.
SCOPE
1.1 This practice covers the analysis of gunshot residue (GSR) by scanning electron microscopy/energy-dispersive X-ray spectrometry (SEM/EDS). The analysis is performed using automated software control of both the SEM and EDS systems, to screen the sample for candidate particles that could be associated with GSR. Manual control of the instrument is then used to perform confirmatory analysis and classification of the candidate particles. This practice refers solely to the analysis of electron microscopy stubs (1).2  
1.2 Since software and hardware formats vary among commercial systems, guidelines will be offered in the most general terms possible. For proper terminology and operation, consult the SEM/EDS system manuals for each instrument.  
1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.4 This standard cannot replace knowledge, skills, or abilities acquired through education, training, and experience (Practice E2917), and is to be used in conjunction with professional judgment by individuals with such discipline-specific knowledge, skills, and abilities.  
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

ABSTRACT
This practice describes the basic instruments, equipments, and supplies used for the medical-legal examination of the victims and/or suspects in sexual assault investigations. Outlined here are the considerations for choosing a variation or optional equipment to be used, in which case all of the types of tools listed herein should be included in any examination facility.
SCOPE
1.1 This specification describes the basic instruments used for the medical-legal examination of victims or suspects, or both, in sexual violence investigations. These specifications are designed to be used in conjunction with other specifications, guides, and practices associated with sexual violence examinations that are listed in Section 2.  
1.2 Additional information can be found in Terminology E1732, Guide E1843, Specifications E2057, and Practice E2123.  
1.3 This standard cannot replace knowledge, skills, or abilities acquired through education, training, and experience (see Practice E2917, Education and Training) and is to be used in conjunction with professional judgement by individuals with such discipline-specific knowledge, skills, and abilities.  
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

ABSTRACT
This guide covers the basic components for the development of a sexual assault investigation protocol, with specific attention to the examination of assault scenes, victims and suspects of sexual assault, the recovery of testimonial, physical, and behavioral evidence, and the preservation and custody of physical evidence. This guide also outlines procedures requiring the experience of experts in a diversity of fields. A multidisciplinary team approach to sexual assault investigation is necessary and is the current standard of care. This team should include members skilled in the following disciplines: law enforcement, criminalistics, victim advocacy, and clinical, forensic practice.
SCOPE
1.1 This guide outlines the basic components for the development of a sexual violence investigation protocols, with specific attention to the examination of sexual violence scenes, victims and suspects of sexual violence, the recovery of testimonial, physical, and behavioral evidence, and the preservation and custody of physical evidence.  
1.2 This guide outlines protocols requiring the experience of experts in a diversity of fields. A multidisciplinary team approach to sexual violence investigation is necessary and is the current standard of care. This team should include members skilled in the following disciplines: law enforcement, criminalistics, victim advocacy, and clinical, forensic practice.  
1.3 This standard cannot replace knowledge, skills, or abilities acquired through education, training, and experience (see Practice E2917, Education and Training) and is to be used in conjunction with professional judgement by individuals with such discipline-specific knowledge, skills, and abilities.  
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
5.1 The procedures put forth in this standard are designed to preserve sexual violence evidence items during collection, storage, and transmittal for analysis at an appropriate laboratory.
SCOPE
1.1 This practice describes the basic considerations that will help preserve different items or types of sexual violence related evidence for subsequent analysis. This practice is designed to be used in conjunction with other specifications, guides, and practices associated with sexual violence examinations that are listed in Section 2.  
1.2 This standard cannot replace knowledge, skills, or abilities acquired through education, training, and experience (see Practice E2917, Education and Training) and is to be used in conjunction with professional judgement by individuals with such discipline-specific knowledge, skills, and abilities.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
5.1 This guide is applicable for all ignitable liquids as defined by Test Method E1618.  
5.2 This guide is applicable to solid and liquid samples (burned and unburned), which are packaged in a closed vapor-tight container (typically a can, fire debris bag, jar, or vial).  
5.3 This guide is to be used in conjunction with the referenced documents by an analyst familiar with the limitations and applicability of each technique used.  
5.4 This guide does not attempt to address all the issues regarding sample extraction and analysis. There could be additional laboratory validated or verified tests, extractions or analyses that can be performed to provide further discrimination and classification of ignitable liquids and ignitable liquid residues present in samples.  
5.5 This guide is intended to be used in conjunction with generally accepted forensic accreditation requirements including documentation and records.
SCOPE
1.1 This guide describes a systematic approach to the extraction, analysis, and classification of ignitable liquids and their residues in solid (for example, fire debris) and liquid samples.  
1.2 This guide addresses evidence handling, extraction methodologies, instrumental analysis techniques, and analytical data interpretation.  
1.3 This guide cannot replace knowledge, skill, or ability acquired through appropriate education, training, and experience and should be used in conjunction with sound professional judgment.  
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
3.1 The procedures outlined herein are grounded in the generally accepted body of knowledge and experience in the field of forensic tape examination and comparison.  
3.2 With successful completion of this tape analysis training program, the trainee gains the theoretical knowledge and practical skills necessary to perform, document, and evaluate forensic tape examinations and comparisons.  
3.3 This training practice covers a variety of instrumental methods which can be used in the analysis of tape. Not all laboratories will have access to all of the instrumentation. It is expected that a tape analysis training program will include all the techniques that are found within a laboratory’s procedures for the forensic examination of tape.  
3.3.1 Instrumental methods that provide organic and inorganic analysis capabilities are utilized in the laboratory training program. Examples include Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy, pyrolysis gas chromatography (PGC), scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM/EDS), X-ray fluorescence (XRF), or X-ray diffraction (XRD).
SCOPE
1.1 This standard is intended as a practice for use by laboratory personnel responsible for training examiners to perform forensic examinations and comparisons on pressure sensitive tapes and adhesives. It contains a list of training objectives with recommended methods of instruction, reading assignments and structured exercises to provide practical experience for the trainee.  
1.1.1 The trainees and training program shall meet or exceed the minimum training requirements set forth in Practice E2917.  
1.1.2 Additional training could be required for a particular method or instrument referred to herein. The application of analytical techniques to tape analysis assumes the trainee is already competent in the use of each particular analytical technique or instrumental method.  
1.1.3 Other sources of information on forensic tape examination not specifically mentioned in this document can be considered and added.  
1.1.4 Additional tape analysis training beyond that which is listed here should be made available to the trainee. Such training could include off-site courses, internships, and specialized training by experienced examiners.  
1.1.5 Continuing education and training is recommended. Additional training provides a forensic tape examiner with the opportunity to remain current in the field.  
1.1.6 Tape samples are evaluated for physical matches of the cut or torn end pieces. This practice does not provide training requirements for physical match comparisons. Additional training is required to conduct this type of analysis.  
1.2 This practice is in a modular format for easy adaptation to an individual laboratory’s training program. Recommendations as to lessons, practical exercises, progress monitoring, and trainee evaluations are included. Reading assignments are listed in each subsequent section of this practice; full citations are available in the References section.  
1.3 A tape analysis training program provides a theoretical foundation and basic practical skills necessary to prepare a trainee to become a qualified forensic tape examiner. At the end of the tape analysis training program, the trainee is capable of forming opinions based upon sound scientific knowledge, appropriate examinations, and practical experience. The trainee also is able to independently work cases, write reports, testify in court, and peer review cases. Upon completion of the program by a trainee or at some regular interval (for example, once per accreditation cycle), the training program should be evaluated for its efficacy and relevance according to the guidance set forth in Practice E2917.  
1.4 This practice does not address human factors (for example, cognitive bias). It is the responsibility of the user of this practice to address human factors during the initial or general t...

SIGNIFICANCE AND USE
3.1 The procedures outlined herein are grounded in the generally accepted body of knowledge and experience in the field of forensic paint examination and comparison.  
3.2 With successful completion of this paint analysis training program, the trainee gains the theoretical knowledge and practical skills necessary to perform, document, and evaluate forensic paint examinations and comparisons.  
3.3 This training practice covers a variety of instrumental methods which can be used in the analysis of paint. Not all laboratories will have access to all of the instrumentation. It is expected that a paint analysis training program will include all the techniques that are found within a laboratory's procedures for the forensic examination of paint.  
3.3.1 Instrumental methods that provide organic and inorganic analysis capabilities are utilized in the laboratory training program. Examples include Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy, pyrolysis gas chromatography (PGC), scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM/EDS), X-ray fluorescence (XRF), and X-ray diffraction (XRD).
SCOPE
1.1 This document is intended as a practice for use by laboratory personnel responsible for training examiners to perform forensic examinations and comparisons of paint. It contains a list of training objectives with recommended methods of instruction, reading assignments and structured exercises to provide practical experience for the trainee.  
1.1.1 The trainees and training program shall meet or exceed the minimum training requirements set forth in Practice E2917.  
1.1.2 Additional training could be required for a particular method or instrument referred to herein. The application of analytical techniques to paint analysis assumes the trainee is already competent in the use of each particular analytical technique or instrumental method.  
1.1.3 Other sources of information on forensic paint examination not specifically mentioned in this document can be considered and added.  
1.1.4 Additional paint analysis training beyond that which is listed here should be made available to the trainee. Such training could include off-site courses, internships, and specialized training by experienced examiners.  
1.1.5 Continuing education and training is recommended. Additional training provides a forensic paint examiner with the opportunity to remain current in the field.  
1.1.6 Paint samples occasionally are evaluated for physical matches of broken edges. This document does not provide training requirements for physical match comparisons. Additional training is required to conduct this type of analysis.  
1.2 This practice is in a modular format for easy adaptation to an individual laboratory’s training program. Recommendations as to lessons, practical exercises, progress monitoring, and trainee evaluations are included. Reading assignments are listed in each subsequent section of this practice; full citations are available in the References section.  
1.3 A paint analysis training program provides a theoretical foundation and basic practical skills necessary to prepare a trainee to become a qualified forensic paint examiner. At the end of the paint analysis training program, the trainee is capable of forming opinions based upon sound scientific knowledge, appropriate examinations, and practical experience. The trainee also is able to independently work cases, write reports, testify in court, and peer review cases. Upon completion of the program by a trainee or at some regular interval (for example, once per accreditation cycle), the training program should be evaluated for its efficacy and relevance according to the guidance set forth in Practice E2917.  
1.4 This standard practice does not address human factors (for example, cognitive bias). It is the responsibility of the user of this standard to address human factors during the initial or general training of...

SIGNIFICANCE AND USE
4.1 This technique is destructive, in that the glass fragments may need to be crushed, and digested in acid.  
4.2 Although the concentration ranges of the calibration curves shown in Appendix X1 are applicable to soda lime and borosilicate glass, this method is useful for the accurate measurement of element concentrations from a wide variety of glass samples.  
4.3 The determination of the element concentrations in glass yields data that can be used to compare fragments.  
4.4 It should be recognized that the method measures the bulk concentration of the target elements. Any extraneous material present on the glass that is not removed before digestion can result in inaccurate concentrations of the measured elements.  
4.5 The precision and accuracy of the method should be established in each laboratory that employs the method.
SCOPE
1.1 One objective of a forensic glass examination is to compare glass samples to determine if they can be discriminated using their physical, optical or chemical properties (for example, color, refractive index (RI), density, elemental composition). If the samples are distinguishable in any of these observed and measured properties, it may be concluded that they did not originate from the same source of broken glass. If the samples are indistinguishable in all of these observed and measured properties, the possibility that they originated from the same source of glass cannot be eliminated. The use of an elemental analysis method such as inductively coupled plasma mass spectrometry yields high discrimination among sources of glass. (1-16)2  
1.2 This test method covers a procedure for quantitative determination of the concentrations of magnesium (Mg), aluminum (Al), iron (Fe), titanium (Ti), manganese (Mn), rubidium (Rb), strontium (Sr), zirconium (Zr), barium (Ba), lanthanum (La), cerium (Ce), neodymium (Nd), samarium (Sm), and lead (Pb) in glass samples.  
1.3 This procedure is applicable to irregularly shaped samples as small as 200 micrograms, for the comparison of fragments of a known source to the recovered fragments from a questioned source. These elements are present in soda lime and borosilicate glass in μg/L to % levels.  
1.4 This procedure is applicable to other elements, other types of glass, and other concentration ranges with appropriate modifications of the digestion procedure (if needed for full recovery of the additional elements), calibration standards and the mass spectrometer conditions. Calcium and potassium, for example, could be added to the list of analytes in a modified analysis scheme. Alternative methods for the determination of concentrations of elements in glass are listed in the references.  
1.5 For any given glass, approximately 40 elements are likely to be present at detectable concentrations using this procedure with minor modifications. The element set stated here is an example of some of these elements that can be detected in glass and used for forensic comparisons.  
1.6 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.7 This standard cannot replace knowledge, skills, or abilities acquired through education, training, and experience and is to be used in conjunction with professional judgment by individuals with such discipline-specific knowledge, skills, and abilities.  
1.8 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.9 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organiz...

SIGNIFICANCE AND USE
4.1 The identification of an ignitable liquid residue in samples from a fire scene can support the field investigator’s opinion regarding the origin, fuel load, and incendiary nature of the fire.  
4.1.1 The identification of an ignitable liquid residue in a fire scene does not necessarily lead to the conclusion that a fire was incendiary in nature. Further investigation can reveal a legitimate reason for the presence of ignitable liquid residues.  
4.1.2 Because of the volatility of ignitable liquids and variations in sampling techniques, the absence of detectable quantities of ignitable liquid residues does not necessarily lead to the conclusion that ignitable liquids were not present at the fire scene.  
4.2 Materials normally found in a building, upon exposure to the heat of a fire, will form pyrolysis and combustion products. Extracted ion profiling and identification of specific compounds or classes of compounds described herein can facilitate the identification of an ignitable liquid in the extract by reducing interference by components generated as products of pyrolysis.
SCOPE
1.1 This test method covers the identification of residues of ignitable liquids in extracts from fire debris samples. Extraction procedures are described in the referenced documents.  
1.2 Although this test method is suitable for all samples, it is especially appropriate for extracts that contain high background levels of substrate materials or pyrolysis and combustion products. This test method is also suitable for the identification of single compounds, simple mixtures, or non-petroleum based ignitable liquids.  
1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.4 This practice cannot replace knowledge, skill, or ability acquired through appropriate education, training, and experience and should be used in conjunction with sound professional judgment.  
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
4.1 This practice is useful for preparing extracts from fire debris for later analysis by gas chromatography mass spectrometry.  
4.2 This is a very sensitive separation procedure, capable of isolating quantities smaller than 1/10 μL of ignitable liquid residue from a sample.
SCOPE
1.1 This practice describes the procedure for separation of small quantities of ignitable liquid residues from samples of fire debris using an adsorbent material to extract the residue from the static headspace above the sample, then eluting the adsorbent with a solvent.  
1.2 While this practice is suitable for successfully extracting ignitable liquid residues over the entire range of concentration, the headspace concentration methods are best used when a high level of sensitivity is required due to a very low concentration of ignitable liquid residues in the sample.  
1.2.1 Unlike other methods of separation and concentration, this practice is essentially nondestructive.  
1.3 Alternate separation and concentration procedures are listed in the referenced documents (see Practices E1386, E1388, E1413, and E2154).  
1.4 This practice does not replace knowledge, skill, ability, experience, education, or training and should be used in conjunction with professional judgment.  
1.5 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
5.1 This technique involves a chemical-precipitation reaction between cocaine and the precipitating reagent. The habit and the aggregation of the crystals formed could be used to distinguish cocaine from other drugs (6).  
5.2 This technique can be utilized on cocaine present in either the salt or free base form.  
5.3 This technique does not distinguish between the salt and free base forms.
SCOPE
1.1 This practice describes procedures applicable to the analysis of cocaine using multiple microcrystal tests (1-6).2  
1.2 These procedures are applicable to cocaine, which is present in solid form or an injectable liquid form. They are not typically applicable to the analysis of cocaine in biological samples.  
1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.4 These procedures could generate observations indicating a positive test for cocaine or its enantiomers which could be incorporated into the analytical scheme as defined by the laboratory.  
1.5 This standard cannot replace knowledge, skills, or abilities acquired through appropriate education, training, and experience (see Practice E2326) and is to be used in conjunction with professional judgment by individuals with such discipline-specific knowledge, skills, and abilities.  
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
5.1 This technique involves a chemical-precipitation reaction between methamphetamine or amphetamine and the precipitating reagent. The habit and the aggregation of the crystals formed could be used to distinguish methamphetamine and amphetamine from other drugs, as well as from each other.
SCOPE
1.1 This practice describes procedures applicable to the analysis of methamphetamine and amphetamine using microcrystal tests (1-6).2  
1.2 These procedures are applicable to methamphetamine and amphetamine, which are present in solid dosage form or an injectable liquid form. These procedures are not typically applicable to the analysis of methamphetamine and amphetamine in biological samples.  
1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.4 These procedures could generate observations indicating a positive test for methamphetamine or amphetamine which could be incorporated into the analytical scheme as defined by the laboratory.  
1.5 This standard cannot replace knowledge, skills, or abilities acquired through appropriate education, training, and experience (see Practice E2326) and is to be used in conjunction with sound professional judgment by individuals with such discipline-specific knowledge, skills, and abilities.  
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
5.1 This technique involves a chemical-precipitation reaction between the phencyclidine or its analogues and the precipitating reagent. The habit and the aggregation of the crystals formed could be used to distinguish phencyclidine or its analogues from other drugs.  
5.2 This technique can be utilized on phencyclidine or its analogues present in either the salt or free base form.  
5.3 This technique does not distinguish between salt and free base forms.
SCOPE
1.1 This practice describes procedures applicable to the analysis of phencyclidine and its analogues using microcrystal tests (1-8).2  
1.2 These procedures are applicable to phencyclidine and its analogues which are present in solid form or in a liquid form. They are not typically applicable to the analysis of phencyclidine and its analogues in biological samples.  
1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.4 These procedures could generate observations indicating a positive test for phencyclidine and its analogues which could be incorporated into the analytical scheme as defined by the laboratory.  
1.5 This standard cannot replace knowledge, skills, or abilities acquired through appropriate education, training, and experience (see Practice E2326) and is to be used in conjunction with sound professional judgment by individuals with such discipline-specific knowledge, skills, and abilities.  
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.