ASTM E2406-09
(Test Method)Standard Test Method for Evaluation of Laundry Sanitizers and Disinfectants for Use in High Efficiency Washing Operations
Standard Test Method for Evaluation of Laundry Sanitizers and Disinfectants for Use in High Efficiency Washing Operations
SIGNIFICANCE AND USE
The procedure in this test method is used to evaluate the effectiveness of a test reagent (antimicrobial agent/active ingredient) or formulation to reduce or completely kill bacterial populations on contaminated fabrics and in wash water following a single wash under simulated low wash volume conditions. (See Table 1.)
SCOPE
1.1 This test method is designed to evaluate sanitizing/disinfectant laundry detergents/additives for use in high efficiency (HE) automatic clothes washing operations that typically utilize very low wash water volumes. This test method is designed to provide testing with representative vegetative bacteria but can also be designed to accommodate the testing of fungi and viruses.
Note 1—Test Method E2274 is the recommended method to evaluate sanitizing/disinfectant laundry detergent/additives for use in traditional high wash water volume automatic clothes washing operations.
1.2 Knowledge of microbiological techniques is required for these procedures.
1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
Note 2—In this method, metric units are used for all applications, except for distance in which case inches are used.
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
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Designation: E2406 − 09
StandardTest Method for
Evaluation of Laundry Sanitizers and Disinfectants for Use
1
in High Efficiency Washing Operations
This standard is issued under the fixed designation E2406; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope E2274Test Method for Evaluation of Laundry Sanitizers
and Disinfectants
1.1 This test method is designed to evaluate sanitizing/
2.2 AATCC Standard:
disinfectant laundry detergents/additives for use in high effi-
AATCC 70-1997Water Repellency; Tumble Jar Dynamic
ciency (HE) automatic clothes washing operations that typi-
3
Absorption Test
cally utilize very low wash water volumes.This test method is
2.3 EPA Standard:
designed to provide testing with representative vegetative
DIS/TSS 13 Laundry Additives—Disinfection and
bacteriabutcanalsobedesignedtoaccommodatethetestingof
Sanitization, U.S. Environmental Protection Agency, Of-
fungi and viruses.
4
fice of Pesticide Programs, April 1980
NOTE 1—Test Method E2274 is the recommended method to evaluate
sanitizing/disinfectant laundry detergent/additives for use in traditional
3. Terminology
high wash water volume automatic clothes washing operations.
3.1 Definitions:
1.2 Knowledge of microbiological techniques is required
3.1.1 active antimicrobial ingredient—a substance added to
for these procedures.
a formulation intended specifically for the inhibition or inac-
1.3 The values stated in SI units are to be regarded as
tivation of microorganisms.
standard. No other units of measurement are included in this
3.1.2 antimicrobial agent(s)—an active ingredient designed
standard.
to suppress the growth or action of microorganisms.
NOTE 2—In this method, metric units are used for all applications,
except for distance in which case inches are used.
3.1.3 carrier count control—procedure used to determine
1.4 This standard does not purport to address all of the
the initial number of microorganisms on a fabric carrier
safety concerns, if any, associated with its use. It is the
following the inoculation and drying procedure.
responsibility of the user of this standard to establish appro-
3.1.4 diluent—sterile deionized water, sterile distilled water
priate safety and health practices and determine the applica-
or sterile synthetic AOAC hard water that may be used to
bility of regulatory limitations prior to use.
prepare the active test formulation, vehicle control or product
control for use in the test procedure.
2. Referenced Documents
2 3.1.5 diluted product solution—test formulation, vehicle
2.1 ASTM Standards:
control, or product control diluted to use concentration.
D1193Specification for Reagent Water
3.1.6 neutralization—aprocessthatresultsinquenchingthe
E177Practice for Use of the Terms Precision and Bias in
antimicrobial activity of a test formulation. This may be
ASTM Test Methods
achieved by dilution of the test formulation(s) to reduce the
E691Practice for Conducting an Interlaboratory Study to
concentration of the antimicrobials, or through the use of
Determine the Precision of a Test Method
chemical agents, called neutralizers, to suppress antibacterial
E1054Test Methods for Evaluation of Inactivators of Anti-
activity.
microbial Agents
3.1.7 numbers control—in assessing sanitizer level
performance, procedure used to determine the number of
1
This test method is under the jurisdiction of ASTM Committee E35 on
Pesticides, Antimicrobials, and Alternative Control Agents and is the direct microorganisms remaining on the fabric carriers and in the
responsibility of Subcommittee E35.15 on Antimicrobial Agents.
Current edition approved Nov. 1, 2009. Published December 2009. Originally
3
approved in 2004. Last previous edition approved in 2004 as E2406–04. DOI: Available from American Association of Textile Chemists and Colorists
10.1520/E2406-09. (AATCC), P.O. Box 12215, Research Triangle Park, NC 27709, http://
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or www.aatcc.org.
4
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM Available from United States Environmental Protection Agency (EPA), Ariel
Standards volume information, refer to the standard’s Document Summary page on Rios Bldg., 1200 Pennsylvania Ave., NW, Washington, DC 20460, http://
the ASTM website. www.epa.gov.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1
---------------------- Page: 1 ----------------------
E2406 − 09
wash water following the test procedure in the presence of the
diluent.Thismayalsobeperformedusingdi
...
This document is not anASTM standard and is intended only to provide the user of anASTM standard an indication of what changes have been made to the previous version. Because
it may not be technically possible to adequately depict all changes accurately, ASTM recommends that users consult prior editions as appropriate. In all cases only the current version
of the standard as published by ASTM is to be considered the official document.
Designation:E2406–04 Designation:E2406–09
Standard Test Method for
Evaluation of Laundry Sanitizers and Disinfectants for Use
1
in High Efficiency Washing Operations
This standard is issued under the fixed designation E2406; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope
1.1 This test method is designed to evaluate sanitizing/disinfectant laundry detergents/additives for use in high efficiency (HE)
automatic clothes washing operations that typically utilize very low wash water volumes.This test method is designed to provide
testing with representative vegetative bacteria but can also be designed to accommodate the testing of fungi and viruses.
NOTE1—Standard test method Test Method 1—Test Method E2274 is the recommended method to evaluate sanitizing/disinfectant laundry
detergent/additives for use in traditional high wash water volume automatic clothes washing operations.
1.2 Knowledge of microbiological techniques is required for these procedures.
1.3In this method metric units are used for all applications, except for distance in which case inches are used.
1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
NOTE 2—In this method, metric units are used for all applications, except for distance in which case inches are used.
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility
of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory
limitations prior to use.
2. Referenced Documents
2
2.1 ASTM Standards:
D1193 Specification for Reagent Water
E177 Practice for Use of the Terms Precision and Bias in ASTM Test Methods
E691 Practice for Conducting an Interlaboratory Study to Determine the Precision of a Test Method
E1054 Test Methods for Evaluation of Inactivators of Antimicrobial Agents
E2274 Test Method for Evaluation of Laundry Sanitizers and Disinfectants
2.2 AATCC Standard:
3
Test Method 70-1997AATCC 70-1997 Water Repellency; Tumble Jar Dynamic Absorption Test
2.3 AOAC Standard:
Official Methods of Analysis of AOAC InternationalChapter 6: Disinfectants, 17th ed., 2000
2.4 EPA Standard:
DIS/TSS 13 Laundry Additives—Disinfection and Sanitization, U.S. Environmental Protection Agency, Office of Pesticide
Programs, April 1980
2.5 Federal Standard:
40 CFR, Part 160Good Laboratory Practice Standards
2.6 Canadian Standard:
4
T-1-215Canadian Pest Management Regulatory Standards Trade Memorandum Oct, 1980.
1
ThistestmethodisunderthejurisdictionofASTMCommitteeE35onPesticidesandAlternativeControlAgentsandisthedirectresponsibilityofSubcommitteeE35.15
on Antibacterial Agents.
Current edition approved Oct. 1, 2004. Published November 2004. DOI: 10.1520/E2406-04.on Antimicrobial Agents.
Current edition approved Nov. 1, 2009. Published December 2009. Originally approved in 2004. Last previous edition approved in 2004 as E2406–04. DOI:
10.1520/E2406-09.
2
ForreferencedASTMstandards,visittheASTMwebsite,www.astm.org,orcontactASTMCustomerServiceatservice@astm.org.For Annual Book of ASTM Standards
volume information, refer to the standard’s Document Summary page on the ASTM website.
3
Available from American Association of Textile Chemists and Colorists (AATCC), One Davis Dr., P.O. Box 12215, Research Triangle Park, NC 27709-2215.27709,
http://www.aatcc.org.
4
Available from AOAC International, Washington, DC.
4
Available from United States Environmental Protection Agency (EPA), Ariel Rios Bldg., 1200 Pennsylvania Ave., NW, Washington, DC 20460, http://www.epa.gov.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.
1
---------------------- Page: 1 ----------------------
E2406–09
3. Terminology
3.1 Definitions:
3.1.1 active antimicrobial ingredient—asubstanceaddedtoaformulationintendedspecificallyfortheinhibitionorinactivation
of microorganisms.
3.1.2 antimicrobial agent(s)—an active ingredient designed to suppress the growth or action of microorganisms.
3.1.3 carrier count control—procedureusedtodeterminetheinitialnumberofmicroorganismsonafabriccarrierfollowingthe
inoculation and drying procedure.
3.1.4 diluent—sterile deionized water, sterile distilled
...
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