ASTM E1839-13
(Test Method)Standard Test Method for Efficacy of Slimicides for the Paper Industry—Bacterial and Fungal Slime
Standard Test Method for Efficacy of Slimicides for the Paper Industry—Bacterial and Fungal Slime
SIGNIFICANCE AND USE
5.1 This test method is to be used to determine if a slime control agent has application in the paper industry for control of bacterial or fungal slime/biofilm.
5.2 This test method is run in acid, alkaline, or acid and alkaline conditions to determine the efficacy of the slime control agent.
5.3 The test conditions may be modified to reflect intended use patterns in typical paper mill systems, including use of actual paper mill furnish.
SCOPE
1.1 This test method presents a procedure to evaluate the efficacy of slimicides for the control of bacterial and fungal slimes in paper mill systems and their counterparts.
1.2 It is the responsibility of the investigator to determine whether Good Laboratory Practices (GLP) are required and to follow them where appropriate (40 CFR 160).
1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
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Designation: E1839 − 13
Standard Test Method for
Efficacy of Slimicides for the Paper Industry—Bacterial and
1
Fungal Slime
This standard is issued under the fixed designation E1839; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 3. Terminology
1.1 This test method presents a procedure to evaluate the 3.1 For definitions of terms related to this practice, see
efficacy of slimicides for the control of bacterial and fungal Terminology E2756.
slimes in paper mill systems and their counterparts.
3.2 Definitions:
1.2 It is the responsibility of the investigator to determine 3.2.1 furnish, n—pulp slurry fed to a paper machine. The
whether Good Laboratory Practices (GLP) are required and to type of pulp (sulfite, Kraft, mechanical), the source of fiber
follow them where appropriate (40 CFR 160). (virgin,recycledincludingpre-orpost-consumerwastepaper),
and the pH are used to designate a specific type of furnish.
1.3 The values stated in SI units are to be regarded as
3.2.2 microbicide, n—aphysicalorchemicalagentthatkills
standard. No other units of measurement are included in this
microorganisms.
standard.
3.2.3 pulp, n—wood separated by chemical or mechanical
1.4 This standard does not purport to address all of the
means into their fibrous components.The pulp is used to make
safety concerns, if any, associated with its use. It is the
paper, paper board, or pulp sheets after specific treatments.
responsibility of the user of this standard to establish appro-
Hardwoodpulpismadefromtrees,suchasmaplesoroaks,and
priate safety and health practices and determine the applica-
softwood pulp is produced from trees, such as pines.
bility of regulatory limitations prior to use.
3.2.4 pulp slurry, n—an aqueous combination of cellulosic
2. Referenced Documents
fibers, fillers, and other additives used for specific grades of
2
2.1 ASTM Standards:
paper.
D1193Specification for Reagent Water
3.2.5 slime, n—biofilm.
E1054Test Methods for Evaluation of Inactivators of Anti-
3.2.6 slimicides, n—chemical agent added during pulp and
microbial Agents
paper processing to reduce the growth of slime-forming
E2756Terminology Relating toAntimicrobial andAntiviral
microorganisms.
Agents
2.2 TAPPI Standard:
4. Summary of Test Method
3
T 205Forming Handsheets for Physical Tests of Pulp
2.3 CFR Standard: 4.1 Bacterial cells or fungal spores are added to acid or
4
alkaline pulp slurries, or both, treated with slimicides to
40 CFR Part 160Good Laboratory Practice Standards
6 7
achieve final concentrations of 2×10 to1×10 bacteria/mL
5 6
and/or 10 to 10 fungal spores/mL, and incubated at appro-
priatetemperaturefordeterminedtimeperiods.Aliquotsofthe
1
This test method is under the jurisdiction of ASTM Committee E35 on
test suspension are then neutralized, plated onto bacterial or
Pesticides,Antimicrobials, andAlternative ControlAgentsand is the direct respon-
fungal medium, and observed for growth. Results with biocide
sibility of Subcommittee E35.15 on Antimicrobial Agents.
Current edition approved April 1, 2013. Published May 2013. Originally are compared to results without biocide (control).
approved in 1996. Last previous edition approved in 2007 as E1839–07. DOI:
4.2 As a performance standard, an effective slimicide is one
10.1520/E1839-13.
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or thatshowsacontinuedreductioninbacterialandfungalcounts
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
relative to the control over the duration of the test.
Standards volume information, refer to the standard’s Document Summary page on
the ASTM website.
3 5. Significance and Use
Available from TechnicalAssociation of the Pulp and Paper Industry (TAPPI),
15 Technology Parkway South, Norcross, GA 30092, http://www.tappi.org.
5.1 This test method is to be used to determine if a slime
4
AvailablefromU.S.GovernmentPrintingOfficeSuperintendentofDocuments,
control agent has application in the paper industry for control
732 N. Capitol St., NW, Mail Stop: SDE, Washington, DC 20401, http://
www.access.gpo.gov. of bacterial or fungal slime/biofilm.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1
---------------------- Page: 1 ----------------------
E1839 − 13
5.2 This test method is run in acid, alkaline, or acid and 6.17 Constant Temperature Shaker—A reliable constant-
alkaline conditions to determine the efficacy of the slime temperature shaker (water bath or incubator type), shall be
control agent. used to provide mixing and aeration and to m
...
This document is not an ASTM standard and is intended only to provide the user of an ASTM standard an indication of what changes have been made to the previous version. Because
it may not be technically possible to adequately depict all changes accurately, ASTM recommends that users consult prior editions as appropriate. In all cases only the current version
of the standard as published by ASTM is to be considered the official document.
Designation: E1839 − 07 E1839 − 13
Standard Test Method for
Efficacy of Slimicides for the Paper Industry—Bacterial and
1
Fungal Slime
This standard is issued under the fixed designation E1839; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope
1.1 This test method presents a procedure to evaluate the efficacy of slimicides for the control of bacterial and fungal slimes
in paper mill systems and their counterparts.
1.2 It is the responsibility of the investigator to determine whether Good Laboratory Practices (GLP) are required and to follow
them where appropriate (40 CFR 160).
1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility
of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory
limitations prior to use.
2. Referenced Documents
2
2.1 ASTM Standards:
D1193 Specification for Reagent Water
E1054 Test Methods for Evaluation of Inactivators of Antimicrobial Agents
E2756 Terminology Relating to Antimicrobial and Antiviral Agents
2.2 TAPPI Standard:
3
T 205 Forming Handsheets for Physical Tests of Pulp
2.3 CFR Standard:
4
Title 40,40 CFR Part 160 Code of Federal Regulations (CFR), Part 160, Good Laboratory Practice Standards
1
This test method is under the jurisdiction of ASTM Committee E35 on Pesticides, Antimicrobials, and Alternative Control Agentsand is the direct responsibility of
Subcommittee E35.15 on Antimicrobial Agents.
Current edition approved April 1, 2007April 1, 2013. Published April 2007May 2013. Originally approved in 1996. Last previous edition approved in 20022007 as
E1839 – 96 (2002).E1839 – 07. DOI: 10.1520/E1839-07.10.1520/E1839-13.
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM Standards
volume information, refer to the standard’s Document Summary page on the ASTM website.
3
Available from Technical Association of the Pulp and Paper Industry (TAPPI), 15 Technology Parkway South, Norcross, GA 30092, http://www.tappi.org.
4
Available from U.S. Government Printing Office,Office Superintendent of Documents, 732 N. Capitol St., NW, Mail Stop: SSOL,SDE, Washington, DC
20402-9328.20401, http://www.access.gpo.gov.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1
---------------------- Page: 1 ----------------------
E1839 − 13
3. Terminology
3.1 For definitions of terms related to this practice, see Terminology E2756.
3.2 Definitions:
3.2.1 furnish, n—pulp slurry fed to a paper machine. The type of pulp (sulfite, Kraft, mechanical), the source of fiber (virgin,
recycled including pre- or post-consumer waste paper), and the pH are used to designate a specific type of furnish.
3.2.2 microbicide, n—a physical or chemical agent that kills microorganisms.
3.2.3 pulp, n—wood separated by chemical or mechanical means into their fibrous components. The pulp is used to make paper,
paper board, or pulp sheets after specific treatments. Hardwood pulp is made from trees, such as maples or oaks, and softwood
pulp is produced from trees, such as pines.
3.2.4 pulp slurry, n—an aqueous combination of cellulosic fibers, fillers, and other additives used for specific grades of paper.
3.2.5 slime, n—biofilm.
3.2.6 slimicides, n—chemical agent added during pulp and paper processing to reduce the growth of slime-forming
microorganisms.
4. Summary of Test Method
4.1 Bacterial cells or fungal spores are added to acid or alkaline pulp slurries, or both, treated with slimicides to achieve final
6 7 5 6
concentrations of 2 × 10 to 1 × 10 bacteria/mL orand/or 10 to 10 fungal spores/mL, and incubated at appropriate temperature
for determined time periods. Aliquots of the test suspension are then neutralized, plated onto bacterial or fungal medium, and
observed for growth. Results with biocide are compared to results without biocide (control).
4.2 As a performance standard, an effective slimicide is one that shows a continued reduction in bacterial and fungal counts
relative to the control over the durat
...
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