ASTM E2149-01
(Test Method)Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents Under Dynamic Contact Conditions (Withdrawn 2010)
Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents Under Dynamic Contact Conditions (Withdrawn 2010)
SIGNIFICANCE AND USE
The antimicrobial activity of a substrate-bound antimicrobial is dependent upon direct contact of microbes with the active chemical agent. This test determines the antimicrobial activity of treated specimen by shaking samples of surface bound materials in a concentrated bacterial suspension for a one hour contact time or other contact times as specified by the investigator. The suspension is serially diluted both . before and after contact and cultured. The number of viable organisms in the suspension is determined and the percent reduction is calculated based on initial counts or on retrievals from appropriate untreated controls.
Note 2—This method is intended for those surfaces having a percent reduction activity of 50 % to 100 % for the specified contact time.
SCOPE
1.1 This test method is designed to evaluate the resistance of non-leaching antimicrobial treated specimens to the growth of microbes under dynamic contact conditions. This dynamic shake flask test was developed for routine quality control and screening tests in order to overcome difficulties in using classical antimicrobial test methods to evaluate substrate-bound antimicrobials. These difficulties include ensuring contact of inoculum to treated surface (as in AATCC 100), flexibility of retrieval at different contact times, use of inappropriately applied static conditions (as in AATCC 147), sensitivity, and reproducibility. This test also allows for the versatility of testing contamination due to such things as hard water, proteins, blood, serum, various chemicals, and other contaminates or physical/chemical stresses or manipulations of the specimens of interest.
1.2 Surface antimicrobial activity is determined by comparing results from the test sample to simultaneously run controls.
1.3 The presence of a leaching antimicrobial is both pre- and post-determined by the presence of a zone of inhibition.
1.4 This test method should be performed only by those trained in microbiological techniques.
1.5 This standard may involve hazardous materials, operations, and equipment. This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
WITHDRAWN RATIONALE
This test method is designed to evaluate the resistance of non-leaching antimicrobial treated specimens to the growth of microbes under dynamic contact conditions. This dynamic shake flask test was developed for routine quality control and screening tests in order to overcome difficulties in using classical antimicrobial test methods to evaluate substrate-bound antimicrobials. These difficulties include ensuring contact of inoculum to treated surface (as in AATCC 100), flexibility of retrieval at different contact times, use of inappropriately applied static conditions (as in AATCC 147), sensitivity, and reproducibility. This test also allows for the versatility of testing contamination due to such things as hard water, proteins, blood, serum, various chemicals, and other contaminates or physical/chemical stresses or manipulations of the specimens of interest.
Formerly under the jurisdiction of Committee E35 on Pesticides and Alternative Control Agents, this test method was withdrawn in January 2010 in accordance with section 10.5.3.1 of the Regulations Governing ASTM Technical Committees, which requires that standards shall be updated by the end of the eighth year since the last approval date.
General Information
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Standards Content (Sample)
NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information.
Designation:E2149–01
Standard Test Method for
Determining the Antimicrobial Activity of Immobilized
1
Antimicrobial Agents Under Dynamic Contact Conditions
This standard is issued under the fixed designation E2149; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope E1054 Test Methods for Evaluation of Inactivators of An-
timicrobial Agents
1.1 Thistestmethodisdesignedtoevaluatetheresistanceof
2.2 Other Documents:
non-leaching antimicrobial treated specimens to the growth of
AATCC Test Method 147-1998 Antibacterial Activity As-
microbes under dynamic contact conditions. This dynamic
sessment of Textile Materials: Parallel Streak Method.
shake flask test was developed for routine quality control and
American Association of Textile Chemists and Colorists,
screening tests in order to overcome difficulties in using
RTP, NC
classical antimicrobial test methods to evaluate substrate-
AATCC Test Method 100-1999 Antibacterial Finishes on
bound antimicrobials. These difficulties include ensuring con-
Fabrics, Evaluation of American Association of Textile
tact of inoculum to treated surface (as in AATCC 100),
Chemists and Colorists, RTP, NC
flexibility of retrieval at different contact times, use of inap-
propriately applied static conditions (as in AATCC 147),
3. Summary of Test Method
sensitivity, and reproducibility. This test also allows for the
3.1 Immobilized antimicrobial agents, such as surface
versatility of testing contamination due to such things as hard
bonded materials, are not free to diffuse into their environment
water, proteins, blood, serum, various chemicals, and other
under normal conditions of use. Test methods such asAATCC
contaminates or physical/chemical stresses or manipulations of
147 that are directly dependent on the ready leachability of the
the specimens of interest.
antimicrobialagentfromthetreatedfabricareinappropriatefor
1.2 Surface antimicrobial activity is determined by compar-
evaluating immobilized antimicrobial agents. The following
ing results from the test sample to simultaneously run controls.
test method ensures good contact between the bacteria and the
1.3 The presence of a leaching antimicrobial is both pre-
treated fiber, fabric, or other substrate by constant agitation of
and post-determined by the presence of a zone of inhibition.
the test specimen in a bacterial suspension during the test
1.4 This test method should be performed only by those
period. The test is suitable for evaluating stressed or modified
trained in microbiological techniques.
specimens when accompanied by adequate controls.
1.5 This standard may involve hazardous materials, opera-
tions, and equipment. This standard does not purport to
NOTE 1—Stresses may include laundry, wear and abrasion, radiation
address all of the safety concerns, if any, associated with its and steam sterilization, UV exposure, solvent manipulation, temperature
susceptibility, or similar physical or chemical manipulation.
use. It is the responsibility of the user of this standard to
establish appropriate safety and health practices and deter-
4. Significance and Use
mine the applicability of regulatory limitations prior to use.
4.1 The antimicrobial activity of a substrate-bound antimi-
2. Referenced Documents
crobial is dependent upon direct contact of microbes with the
2
active chemical agent. This test determines the antimicrobial
2.1 ASTM Standards:
activity of treated specimen by shaking samples of surface
bound materials in a concentrated bacterial suspension for a
1
This test method is under the jurisdiction of ASTM Committee E35 on
one hour contact time or other contact times as specified by the
Pesticides and is the direct responsibility of Subcommittee E35.15 onAntimicrobial
investigator. The suspension is serially diluted both . before
Agents.
andaftercontactandcultured.Thenumberofviableorganisms
Current edition approved June 10, 2001. PublishedAugust 2001. DOI: 10.1520/
E2149-01.
in the suspension is determined and the percent reduction is
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
calculated based on initial counts or on retrievals from appro-
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
priate untreated controls.
Standards volume information, refer to the standard’s Document Summary page on
the ASTM website.
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E2149–01
NOTE 2—This method is intended for those surfaces having
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