ASTM E723-97(2002)

Standard Test Method for Efficacy of Antimicrobials as Preservatives for Aqueous-Based Products Used in the Paper Industry (Bacterial Spoilage)

SCOPE
1.1 This laboratory test method is used to determine the efficacy of an antimicrobial for preventing bacterial spoilage of in-process aqueous-based products used in the paper industry. For information on fungal spoilage, see Test Method E 875. This test method should be performed by persons who have had basic microbiological training.  
1.2  This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

General Information

• Status: Historical
• Publication Date: 09-Apr-1997
• ICS: 85.020 - Paper production processes
• Technical Committee: E35 - Pesticides, Antimicrobials, and Alternative Control Agents
• Drafting Committee: E35.15 - Antimicrobial Agents

Relations

• Replaces: ASTM E723-97 - Standard Test Method for Efficacy of Antimicrobials as Preservatives for Aqueous-Based Products Used in the Paper Industry (Bacterial Spoilage)
• Effective Date: 10-Apr-1997
• Replaced By: ASTM E723-07 - Standard Test Method for Efficacy of Antimicrobials as Preservatives for Aqueous-Based Products Used in the Paper Industry (Bacterial Spoilage)
• Effective Date: 01-Apr-2007
• Referred By: ASTM E875-20 - Standard Practice for Evaluation of Fungal Control Agents as Preservatives for Aqueous-Based Products Used in the Paper Industry
• Effective Date: 10-Apr-1997

Standards Content (Sample)

NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation:E723–97 (Reapproved 2002)
Standard Test Method for
Efficacy of Antimicrobials as Preservatives for Aqueous-
Based Products Used in the Paper Industry (Bacterial
1
Spoilage)
This standard is issued under the fixed designation E 723; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (e) indicates an editorial change since the last revision or reapproval.
1. Scope 3.1.2 bactericide, n—an agent that kills bacteria. This term
is applied to chemical agents that kill bacteria but not neces-
1.1 This laboratory test method is used to determine the
sarily bacterial spores.
efficacy of an antimicrobial for preventing bacterial spoilage of
3.1.3 preservatives, n—a chemical or physical agent used to
in-process aqueous-based products used in the paper industry.
prevent microbial spoilage of products.
For information on fungal spoilage, see Test Method E 875.
This test method should be performed by persons who have
4. Summary of Test Method
had basic microbiological training.
4.1 Aqueous material to be preserved is inoculated with an
1.2 This standard does not purport to address all of the
appropriate microbial inoculum followed by addition of a
safety concerns, if any, associated with its use. It is the
concentration of bactericide that will kill the microbes and
responsibility of the user of this standard to establish appro-
prevent their growth for a desired period of time. Microbial
priate safety and health practices and determine the applica-
numbers in the sample are determined at various time periods
bility of regulatory limitations prior to use.
andcomparedtoacontolwithoutanybiocide.Theproperlevel
2. Referenced Documents of antimicrobial is one that reduces and keeps the organisms to
2
an acceptable level in the test material.
2.1 ASTM Standards:
D 1193 Specification for Reagent Water
5. Significance and Use
E 875 TestMethodforEfficacyofFungalControlAgentsas
5.1 This test method should be used to determine if an
Preservatives for Aqueous Based Products Used in the
antimicrobial prevents spoilage by bacteria and preserves
Paper Industry
pigment suspensions, dye solutions, pulp slurries, starch solu-
E 1054 Practices for Evaluating Inactivators of Antimicro-
tions, polymers, sizing agents, latex emulsions, and other
bial Agents Used in Disinfectant, Sanitizer, Antiseptic, or
aqueous-based materials used in the paper industry.
Preserved Products
E 1326 Guide for Evaluating Nonconventional Microbio-
6. Apparatus
logical Tests Used for Enumerating Bacteria
6.1 Balance—Two balances: one should be sensitive to 0.1
g at a load of 200 g and have a platform to accomodate bottles
3. Terminology
being used in the test. The second balance (analytical) should
3.1 Definitions of Terms Specific to This Standard:
be sensitive to 0.1 mg and should be employed to weigh the
3.1.1 antimicrobial, n—chemicalorphysicalagentthatkills
candidatepreservativetobeusedinthepreparationofthestock
microorganisms.
solutions.
6.2 Bottles—Borosilicateglassmilkdilutionbottlesorother
suitable containers fitted either with screw caps or Escher
1
This test method is under the jurisdiction of ASTM Committee E35 on
rubber stoppers. These bottles are used for water blanks and
Pesticides and Alternative Control Agents and is the direct responsibility of
aqueous-based samples.
Subcommittee E35.15 on Antibacterial and Antiviral Agents.
6.3 Colony Counter—Anyoneofseveraltypesmaybeused
Current edition approved April 10, 1997. Published June 1997. Originally
published as E 723 – 80. Last previous edition E 723 – 91.
as the Quebec, Buck, and Wolfhuegel. A hand tally for the
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
recording of the bacterial count is recommended if manual
contact ASTM Customer Service at [e-mail]. For Annual Book of ASTM
counting is done. Alternatively, an automated video colony
Standards volume information, refer to the standard’s Document Summary page on
the ASTM website. counter may also be used.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.
1

---------------------- Page: 1 ----------------------
E723–97 (2002)
6.4 Culture Tube Closures—Appropriate nontoxic closures 8.3 The material under test should be inoculated with
should be selected. sufficient microorganisms either from pure cultures or con-
6.5 Culture Tubes—Recommended size is 15 by 125 mm or tained in the spoiled material used as the inoculum to give a
18 by 150 mm without lip, and preferably of borosilicate glass. bacterial count of 1 000 000/mL or higher.
6.6 Blender—Any blender that will assure proper agitation
9. Procedure
an
...