ASTM D4300-98a

Standard Test Methods for Ability of Adhesive Films to Support or Resist the Growth of Fungi

SCOPE
1.1 These test methods test the ability of adhesive films to inhibit or support the growth of selected fungal species growing on agar plates by providing means of testing the films on two agar substrates, one which promotes microbial growth, and one which does not.
1.2 These test methods are not appropriate for all adhesives. The activity of certain biocides may not be demonstrated by these test methods as a result of irreversible reaction with some of the medium constituents.
Note 1—As an example, quaternary ammonium compounds are inactivated by agar.
1.3 A test method is included for use with low-viscosity adhesives along with an alternative method for use with mastic-type adhesives. Also, a method approved by the government is given.
1.4 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. These test methods are designed to be used by persons trained in correct microbiological techniques. Specific precautionary statements are given in Section 7 and in 14.3.2.

General Information

• Status: Historical
• Publication Date: 09-Oct-2001
• ICS: 83.180 - Adhesives
• Technical Committee: D14 - Adhesives
• Drafting Committee: D14.30 - Wood Adhesives

Relations

• Replaced By: ASTM D4300-01 - Standard Test Methods for Ability of Adhesive Films to Support or Resist the Growth of Fungi
• Effective Date: 10-Oct-2001

Standards Content (Sample)

NOTICE: This standard has either been superseded and replaced by a new version or
withdrawn. Contact ASTM International (www.astm.org) for the latest information.
Designation: D 4300 – 98a
AMERICAN SOCIETY FOR TESTING AND MATERIALS
100 Barr Harbor Dr., West Conshohocken, PA 19428
Reprinted from the Annual Book of ASTM Standards. Copyright ASTM
Standard Test Methods for
Ability of Adhesive Films to Support or Resist the Growth of
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Fungi
This standard is issued under the fixed designation D 4300; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (e) indicates an editorial change since the last revision or reapproval.
This standard has been approved for use by agencies of the Department of Defense.
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1. Scope Polymeric Materials to Fungi
2.2 TAPPI Method:
1.1 These test methods test the ability of adhesive films to
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T487 Fungus Resistance for Paper and Paperboard
inhibit or support the growth of selected fungal species
growing on agar plates by providing means of testing the films
3. Terminology
on two agar substrates, one which promotes microbial growth,
3.1 Definitions:
and one which does not.
3.1.1 resist, vt, as related to fungi—to fend off or withstand
1.2 These test methods are not appropriate for all adhesives.
the growth.
The activity of certain biocides may not be demonstrated by
3.1.2 support, vt, as related to fungi—to submit to the
these test methods as a result of irreversible reaction with some
growth.
of the medium constituents.
3.2 Definitions of Terms Specific to This Standard:
NOTE 1—As an example, quaternary ammonium compounds are inac-
3.2.1 adhesive preparation, n—the adhesive as packaged
tivated by agar.
for distribution, storage, and use.
1.3 A test method is included for use with low-viscosity
3.2.2 adhesive film, n—the small portion of the adhesive
adhesives along with an alternative method for use with
preparation, as prepared for use by the consumer, either with
mastic-type adhesives. Also, a method approved by the gov-
additives or as received, which is cast on a substrate, cured 24
ernment is given.
h, and represents the glue line.
1.4 The values stated in SI units are to be regarded as the
3.2.2.1 Discussion—For purposes of these test methods the
standard. The values in parentheses are for information only.
adhesive film is the thin layer of adhesive spread on either the
1.5 This standard does not purport to address all of the
21-mm fiberglass disk as described in 14.2, or the adhesive
safety concerns, if any, associated with its use. It is the
layer 3 mm thick which is cast on the tile squares as described
responsibility of the user of this standard to establish appro-
in 15.1.
priate safety and health practices and determine the applica-
3.2.3 zone of inhibition, n—the area on an inoculated agar
bility of regulatory limitations prior to use. These test methods
plate surrounding the adhesive-coated disk or tile, showing a
are designed to be used by persons trained in correct micro-
reduced fungal growth or an absence thereof.
biological techniques. Specific precautionary statements are
3.3 Abbreviations:
given in Section 7 and in Note 9.
3.3.1 PDA—potato dextrose agar.
3.3.2 MSA—mineral salts agar.
2. Referenced Documents
3.3.3 ZI—zone of inhibition.
2.1 ASTM Standards:
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4. Significance and Use
D 907 Terminology of Adhesives
D 1286 Test Method for Effect of Mold Contamination on
4.1 These test methods are designed to be used to determine
Permanence of Adhesive Preparations and Adhesive
the susceptibility of the adhesive film to biodegradation and
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Bonds
whether the adhesive will carry into the glue line sufficient
G 21 Practice for Determining Resistance of Synthetic
anti-fungal properties to prevent growth of fungi frequently
present on the gluing equipment, on adherends, or in the
adhesive as applied.
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These test methods are under the jurisdiction of ASTM Committee D-14 on
4.2 Potato dextrose agar (PDA) provides a complete me-
Adhesives and are the direct responsibility of Subcommittee D14.30 on Wood
Adhesives.
dium for the growth of fungi, while mineral salts agar (MSA)
Current edition approved Sept. 10, 1998. Published March 1999. Originally
lacks a carbohydrate source and provides a less favorable
published as D 4300 – 83. Last previous edition D 4300 – 98. Replaces D 1286 – 57
(1979).
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Annual Book of ASTM Standards, Vol 15.06.
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3 Annual Book of ASTM Standards, Vol 14.02.
Discontinued; see 1983 Annual Book of Standards, Vol 15.06.
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Available from TAPPI, P. O. Box 105113, Atlanta, GA 30348.
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D 4300
medium. Use of PDA tests the adhesive film for its ability to 5.1.7 Glove Bag, 68 cm in length and width, 38 cm in
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resist the growth of fungi on its surface as well as its ability to height.
repel a copious growth of fungi on the adjacent agar surf
...