ASTM D4300-23

Standard Test Methods for Ability of Adhesive Films to Support or Resist the Growth of Fungi

SIGNIFICANCE AND USE
4.1 These test methods are designed to be used to determine the susceptibility of the adhesive film to biodegradation and whether the adhesive will carry into the bond line sufficient anti-fungal properties to prevent growth of fungi frequently present on the gluing equipment, on adherends, or in the adhesive as applied.  
4.2 Potato dextrose agar (PDA) provides a complete medium for the growth of fungi, while mineral salts agar (MSA) lacks a carbohydrate source and provides a less favorable medium. Use of PDA tests the adhesive film for its ability to resist the growth of fungi on its surface as well as its ability to repel a copious growth of fungi on the adjacent agar surface. Use of MSA tests the adhesive film primarily for its ability to resist the growth of fungi on its surface. When it is used, there is a reduced possibility that the growth from the agar will be mis-read as coming from the adhesive film, since fungal growth on the adjacent agar will be scant.
Note 2: The method given here using the MSA is based on Practice G21, adapted to be used with adhesives. Requirements for the use of the MSA are described in 10.2, and a mixed species of fungi is prescribed in 8.2 for the inoculum.  
4.3 The results obtained when using the procedures given in this method apply only to the species used for the testing. The test species listed in Section 8 are frequently used by laboratories to test for antifungal properties, but they are not the only ones which could be used. Selection of the fungal species to test against requires informed judgment by the testing laboratory or by the party requesting the tests. These methods are especially useful when species that have been isolated from contaminated adhesives are used as the test species (see Section 8) to aid in the selection of more effective fungicides.  
4.4 The efficacy of some biocides may change in storage due to the chemical and thermal environment to which they are subjected as components of certain adhesi...
SCOPE
1.1 These test methods test the ability of adhesive films to inhibit or support the growth of selected fungal species growing on agar plates by providing means of testing the films on two agar substrates, one which promotes microbial growth, and one which does not.  
1.2 These test methods are not appropriate for all adhesives. The activity of certain biocides may not be demonstrated by these test methods as a result of irreversible reaction with some of the medium constituents.  
Note 1: As an example, quaternary ammonium compounds are inactivated by agar.  
1.3 A test method is included for use with low-viscosity adhesives along with an alternative method for use with mastic-type adhesives.  
1.4 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.  
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. These test methods are designed to be used by persons trained in correct microbiological techniques. Specific precautionary statements are given in Section 7 and in 14.3.2.  
1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

General Information

• Status: Published
• Publication Date: 30-Apr-2023
• ICS: 83.180 - Adhesives
• Technical Committee: D14 - Adhesives
• Drafting Committee: D14.30 - Wood Adhesives

Standards Content (Sample)

This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the
Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
Designation: D4300 − 23
Standard Test Methods for
Ability of Adhesive Films to Support or Resist the Growth of
1
Fungi
This standard is issued under the fixed designation D4300; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
This standard has been approved for use by agencies of the U.S. Department of Defense.
1. Scope* 2. Referenced Documents
2
1.1 These test methods test the ability of adhesive films to
2.1 ASTM Standards:
inhibit or support the growth of selected fungal species D907 Terminology of Adhesives
growing on agar plates by providing means of testing the films
G21 Practice for Determining Resistance of Synthetic Poly-
on two agar substrates, one which promotes microbial growth, meric Materials to Fungi
and one which does not.
3
2.2 TAPPI Method:
1.2 These test methods are not appropriate for all adhesives. T487 Fungus Resistance for Paper and Paperboard
The activity of certain biocides may not be demonstrated by
these test methods as a result of irreversible reaction with some
3. Terminology
of the medium constituents.
3.1 Definitions—Many terms in this test method are defined
in Terminology D907.
NOTE 1—As an example, quaternary ammonium compounds are
inactivated by agar. 3.2 Definitions of Terms Specific to This Standard:
3.2.1 adhesive preparation, n—the adhesive as packaged for
1.3 A test method is included for use with low-viscosity
distribution, storage, and use.
adhesives along with an alternative method for use with
mastic-type adhesives.
3.2.2 adhesive film, n—the small portion of the adhesive
preparation, as prepared for use by the consumer, either with
1.4 The values stated in SI units are to be regarded as the
additives or as received, which is cast on a substrate, cured
standard. The values given in parentheses are for information
24 h, and represents the glue line.
only.
3.2.2.1 Discussion—For purposes of these test methods the
1.5 This standard does not purport to address all of the
adhesive film is the thin layer of adhesive spread on either the
safety concerns, if any, associated with its use. It is the
21 mm fiberglass disk as described in 14.2, or the adhesive
responsibility of the user of this standard to establish appro-
layer 3 mm thick which is cast on the tile squares as described
priate safety, health, and environmental practices and deter-
in 15.1.
mine the applicability of regulatory limitations prior to use.
These test methods are designed to be used by persons trained 3.2.3 zone of inhibition, n—the area on an inoculated agar
in correct microbiological techniques. Specific precautionary plate surrounding the adhesive-coated disk or tile, showing a
statements are given in Section 7 and in 14.3.2. reduced fungal growth or an absence thereof.
1.6 This international standard was developed in accor-
3.3 Abbreviations:
dance with internationally recognized principles on standard-
3.3.1 PDA—potato dextrose agar.
ization established in the Decision on Principles for the
3.3.2 MSA—mineral salts agar.
Development of International Standards, Guides and Recom-
mendations issued by the World Trade Organization Technical
3.3.3 ZI—zone of inhibition.
Barriers to Trade (TBT) Committee.
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
1
These test methods are under the jurisdiction of ASTM Committee D14 on contact ASTM Customer Service at [e-mail]. For Annual Book of ASTM
Adhesives and are the direct responsibility of Subcommittee D14.30 on Wood Standards volume information, refer to the standard’s Document Summary page on
Adhesives. the ASTM website.
3
Current edition approved May 1, 2023. Published May 2023. Originally Available from Technological Association of the Pulp and Paper Industry
ɛ1
approved in 1983. Last previous edition approved in 2021 as D4300 – 01 (2021) . (TAPPI), 15 Technology Parkway South, Suite 115, Peachtree Corners, GA 30092,
DOI: 10.1520/D4300-23. http://www.tappi.org.
*A Summary of Changes section appears at the end of this standard
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

---------------------- Page: 1 ----------------------
D4300 − 23
4. Significance and Use 5.1.3 Filter Disk, Glass Microfibre, 934-AM, diameter-
4
21 mm.
4.1 These test methods are designed to be used to determine
4
5.1.4 Filter Disk, Sterile Whatman No. 1.
the susceptibility of the adhesive film to biodegradation and
5.1.5 Fil
...

This document is not an ASTM standard and is intended only to provide the user of an ASTM standard an indication of what changes have been made to the previous version. Because
it may not be technically possible to adequately depict all changes accurately, ASTM recommends that users consult prior editions as appropriate. In all cases only the current version
of the standard as published by ASTM is to be considered the official document.
´1
Designation: D4300 − 01 (Reapproved 2021) D4300 − 23
Standard Test Methods for
Ability of Adhesive Films to Support or Resist the Growth of
1
Fungi
This standard is issued under the fixed designation D4300; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
This standard has been approved for use by agencies of the U.S. Department of Defense.
1
ε NOTE—Editorial changes were made to 8.1 and 8.2 in April 2021.
1. Scope Scope*
1.1 These test methods test the ability of adhesive films to inhibit or support the growth of selected fungal species growing on agar
plates by providing means of testing the films on two agar substrates, one which promotes microbial growth, and one which does
not.
1.2 These test methods are not appropriate for all adhesives. The activity of certain biocides may not be demonstrated by these
test methods as a result of irreversible reaction with some of the medium constituents.
NOTE 1—As an example, quaternary ammonium compounds are inactivated by agar.
1.3 A test method is included for use with low-viscosity adhesives along with an alternative method for use with mastic-type
adhesives. Also, a method approved by the government is given.
1.4 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility
of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of
regulatory limitations prior to use. These test methods are designed to be used by persons trained in correct microbiological
techniques. Specific precautionary statements are given in Section 7 and in 14.3.2.
1.6 This international standard was developed in accordance with internationally recognized principles on standardization
established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued
by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
2. Referenced Documents
2
2.1 ASTM Standards:
D907 Terminology of Adhesives
D1286 Method of Test for Effect of Mold Contamination on Permanence of Adhesives Preparations and Adhesives Bonds
3
(Withdrawn 1983)
1
These test methods are under the jurisdiction of ASTM Committee D14 on Adhesives and are the direct responsibility of Subcommittee D14.30 on Wood Adhesives.
Current edition approved April 1, 2021May 1, 2023. Published April 2021May 2023. Originally approved in 1983. Last previous edition approved in 20132021 as
ɛ1
D4300 – 01 (2013).(2021) . DOI: 10.1520/D4300-01R21E01.10.1520/D4300-23.
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or contact ASTM Customer Service at [e-mail]. For Annual Book of ASTM Standards
volume information, refer to the standard’s Document Summary page on the ASTM website.
*A Summary of Changes section appears at the end of this standard
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

---------------------- Page: 1 ----------------------
D4300 − 23
G21 Practice for Determining Resistance of Synthetic Polymeric Materials to Fungi
3
2.2 TAPPI Method:
T487 Fungus Resistance for Paper and Paperboard
3. Terminology
3.1 Definitions—Many terms in this test method are defined in Terminology D907.
3.2 Definitions of Terms Specific to This Standard:
3.2.1 adhesive preparation, n—the adhesive as packaged for distribution, storage, and use.
3.2.2 adhesive film, n—the small portion of the adhesive preparation, as prepared for use by the consumer, either with additives
or as received, which is cast on a substrate, cured 24 h, 24 h, and represents the glue line.
3.2.2.1 Discussion—
For purposes of these test methods the adhesive film is the thin layer of adhesive spread on either the 21-mm21 mm fiberglass disk
as described in 14.2, or the adhesive layer 3 mm thick which is cast on the tile squares as described in 15.1.
3.2.3 zone of inhibition, n—the area on an inoculated agar plate surrounding the adhesive-coated disk or tile, showing a reduced
fungal growth or an absence thereof.
3.3 Abbreviations:
3.3.1 PDA—potato dextrose agar.
3.3.2 MSA—mineral salts
...