Standard Test Method for Determining the Resistance of Paint Films and Related Coatings to Algal Defacement

SIGNIFICANCE AND USE
Defacement of paint and coating films by algal growth is a common phenomenon under certain conditions. It is generally known that differences in the environment, lighting, temperature, substrate, and other factors in addition to the coating composition affect the susceptibility of a given painted surface. This test method attempts to provide a means to comparatively evaluate different coating formulations for their relative performance under a given set of conditions. It does not imply that a coating that resists growth under these conditions will necessarily resist growth in the actual application (see Note 1).
Familiarity with microbiological techniques is required. This test method should not be used by persons without at least basic microbiological training.
SCOPE
1.1 This test method covers an accelerated method for determining the relative resistance of a paint or coating film to algal growth.
Note 1—It is hoped that a ranking of relative performance would be similar to that ranked from outdoor exposures. However, this test method should not be used as a replacement for exterior exposure since many other factors, only a few of which are listed will affect those results.  
1.2 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

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NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation: D5589 − 09
StandardTest Method for
Determining the Resistance of Paint Films and Related
1
Coatings to Algal Defacement
This standard is issued under the fixed designation D5589; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope a mixture of the proper algal species, (3) expose the inoculated
samples under the appropriate conditions for growth, and (4)
1.1 This test method covers an accelerated method for
provide a schedule and guidelines for visual growth ratings.
determining the relative resistance of a paint or coating film to
This test method is not designed to include all the necessary
algal growth.
procedures to maintain the proper microbiological techniques
NOTE 1—It is hoped that a ranking of relative performance would be
required to provide the most accurate results.
similar to that ranked from outdoor exposures. However, this test method
should not be used as a replacement for exterior exposure since many
4. Significance and Use
other factors, only a few of which are listed will affect those results.
4.1 Defacementofpaintandcoatingfilmsbyalgalgrowthis
1.2 The values stated in SI units are to be regarded as the
standard. The values given in parentheses are for information a common phenomenon under certain conditions. It is gener-
ally known that differences in the environment, lighting,
only.
temperature, substrate, and other factors in addition to the
1.3 This standard does not purport to address all of the
coating composition affect the susceptibility of a given painted
safety concerns, if any, associated with its use. It is the
surface. This test method attempts to provide a means to
responsibility of the user of this standard to establish appro-
comparatively evaluate different coating formulations for their
priate safety and health practices and determine the applica-
relative performance under a given set of conditions. It does
bility of regulatory limitations prior to use.
not imply that a coating that resists growth under these
2. Referenced Documents
conditions will necessarily resist growth in the actual applica-
2
tion (see Note 1).
2.1 ASTM Standards:
D822 Practice for Filtered Open-Flame Carbon-Arc Expo-
4.2 Familiarity with microbiological techniques is required.
sures of Paint and Related Coatings
This test method should not be used by persons without at least
D4141 Practice for Conducting Black Box and Solar Con-
basic microbiological training.
centrating Exposures of Coatings
D4587 Practice for Fluorescent UV-Condensation Expo-
5. Apparatus and Materials
sures of Paint and Related Coatings
5.1 Balance, capable of weighing to 0.10 g.
D5031 Practice for Enclosed Carbon-Arc Exposure Tests of
Paint and Related Coatings
5.2 Incubator, or other device capable of maintaining a
D6695 Practice for Xenon-Arc Exposures of Paint and
constant temperature between 25 6 2°C, relative humidity of
Related Coatings
≥85 %, and having a constant fluorescent light source.
5.3 Refrigerator.
3. Summary of Test Method
3.1 This test method outlines a procedure to (1) prepare a 5.4 Petri Dishes, 100 by 15 mm (3.9 by 0.6 in.).
suitable specimen for testing, (2) inoculate the specimen with
5.5 Autoclave.
1 3
5.6 Paint Brush, coarse bristle, 12 to 19 mm ( ⁄2 to ⁄4 in.).
1
This test method is under the jurisdiction of ASTM Committee D01 on Paint
and Related Coatings, Materials, andApplications and is the direct responsibility of
5.7 Test Substrate, filter paper, either regular paper or glass
Subcommittee D01.28Biodeterioration.
fiber, 4.2 cm (1.65 in.) in diameter, or drawdown paper
Current edition approved July 1, 2009. Published August 2009. Originally
approved in 1994. Last previous edition approved in 2002 as D5589 – 97 (2002).
(unlaquered chart paper) 21.6 by 28.0 cm (8.5 by 11 in.), cut
DOI: 10.1520/D5589-09.
into ten 21.6 by 2.8-cm (8.5 by 1.1-in.) strips may be used.
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
5.8 Tissue Grinder.
Standards volume information, refer to the standard’s Document Summary page on
the ASTM website. 5.9 Atomizer or Chromatography Sprayer.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

---------------------- Page: 1 ----------------------
D5589 − 09
5.10 Sterile Glass Rods, Forceps, 250-mL Glass Erlenmeyer 6.3.2 Allen’s Agar—Prepare by dissolving 15 g of agar in
Flask, and other routine microbiological equipment. 1000 mL Allen’s Medium before autoclaving. Cool to 45 to
3 4 50°C be
...

This document is not anASTM standard and is intended only to provide the user of anASTM standard an indication of what changes have been made to the previous version. Because
it may not be technically possible to adequately depict all changes accurately, ASTM recommends that users consult prior editions as appropriate. In all cases only the current version
of the standard as published by ASTM is to be considered the official document.
Designation:D5589–97(Reapproved2002) Designation:D5589–09
Standard Test Method for
Determining the Resistance of Paint Films and Related
1
Coatings to Algal Defacement
This standard is issued under the fixed designation D 5589; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope
1.1 This test method covers an accelerated method for determining the relative resistance of a paint or coating film to algal
growth.
NOTE 1—It is hoped that a ranking of relative performance would be similar to that ranked from outdoor exposures. However, this test method should
not be used as a replacement for exterior exposure since many other factors, only a few of which are listed will affect those results.
1.2 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility
of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory
limitations prior to use.
2. Referenced Documents
2
2.1 ASTM Standards:
D 822 Practice for Filtered Open-Flame Carbon-Arc Exposures of Paint and Related Coatings
D 4141 Practice for Conducting Black Box and Solar Concentrating Exposures of Coatings
D 4587 Practice for Fluorescent UV-Condensation Exposures of Paint and Related Coatings
2
D 5031 Practice for Enclosed Carbon-Arc Exposure Tests of Paint and Related Coatings
G53Practice for Operating Light- and Water-Exposure Apparatus (Fluorescent UV-Condensation Type) for Exposure of
Nonmetallic Materials Practice for Enclosed Carbon-Arc Exposure Tests of Paint and Related Coatings
D 6695 Practice for Xenon-Arc Exposures of Paint and Related Coatings
3. Summary of Test Method
3.1 This test method outlines a procedure to (1) prepare a suitable specimen for testing, (2) inoculate the specimen with a
mixture of the proper algal species, (3) expose the inoculated samples under the appropriate conditions for growth, and (4) provide
a schedule and guidelines for visual growth ratings. This test method is not designed to include all the necessary procedures to
maintain the proper microbiological techniques required to provide the most accurate results.
4. Significance and Use
4.1 Defacement of paint and coating films by algal growth is a common phenomenon under certain conditions. It is generally
knownthatdifferencesintheenvironment,lighting,temperature,substrate,andotherfactorsinadditiontothecoatingcomposition
affectthesusceptibilityofagivenpaintedsurface.Thistestmethodattemptstoprovideameanstocomparativelyevaluatedifferent
coating formulations for their relative performance under a given set of conditions. It does not imply that a coating that resists
growth under these conditions will necessarily resist growth in the actual application (see Note 1).
4.2 Familiarity with microbiological techniques is required. This test method should not be used by persons without at least
basic microbiological training.
5. Apparatus and Materials
5.1 Balance, capable of weighing to 0.10 g.
5.2 Incubator, or other device capable of maintaining a constant temperature between 25 6 2°C, relative humidity of$85 %,
1
This test method is under the jurisdiction of ASTM Committee D01 on Paint and Related Coatings, Materials, and Applications and is the direct responsibility of
Subcommittee D01.28 Biodeterioration.
Current edition approved July 10, 1997. Published September 1997. Originally published as D5589–94. Last previous edition D5589–94.
Current edition approved July 1, 2009. Published August 2009. Originally approved in 1994. Last previous edition approved in 2002 as D 5589 – 97 (2002).
2
For referencedASTM standards, visit theASTM website, www.astm.org, or contactASTM Customer Service at service@astm.org. For Annual Book of ASTM Standards
, Vol 06.01.volume information, refer to the standard’s Document Summary page on the ASTM website.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.
1

---------------------- Page: 1 ----------------------
D5589–09
and having a constant fluorescent light source.
5.3 Refrigerator.
5.4 Petri Dishes, 100 by 15 mm (3.9 by 0.6 in.).
5.5 Au
...

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