D01.28 - Biodeterioration

SIGNIFICANCE AND USE
5.1 An accelerated test for determining the resistance of interior coated building products to mold growth is useful in estimating the relative performance for use in interior environments under conditions favorable to fungal growth.  
5.2 Static or environmental chambers provide controlled laboratory micro-environment conditions. These chambers are not intended to duplicate room conditions, and care must be taken when interpreting the results. Static chambers are not a substitute for dynamic chambers or field studies.
SCOPE
1.1 This test method covers an environmental chamber and the conditions of operation to evaluate in a 4-week period the relative resistance to mold growth and microbial surface defacement on coated building products designed for interior application using an indirect inoculation method. The apparatus is designed so it can be easily built or obtained by any interested party.  
1.2 This test method can be used to evaluate the comparative resistance of coated building products to accelerated mold growth. Ratings do not imply a specific time period that the coated building product will be free of fungal growth during installation in an interior environment.  
1.3 This test method is not intended for use in the evaluation of public health claims.  
1.4 The test method is intended for the accelerated evaluation of mold growth on a coated building product designed for interior use. This method is not intended for evaluation of surfaces designed for exterior applications or uncoated surfaces. Use of this test method for evaluating exterior performance has not been validated, nor have the limitations for such use been determined.  
1.5 The values stated in either SI units or inch-pound units are to be regarded separately as standard. The values stated in each system may not be exact equivalents; therefore, each system shall be used independently of the other. Combining values from the two systems may result in non-conformance with the standard.  
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
4.1 The growth of fungi in and on the surface of paint films represents a major cause of discoloration or disfigurement of painted surfaces. Because of their dark pigmentation, it is frequently difficult to distinguish fungi from dirt or soil particles.  
4.2 Use of Pictorial Standards:  
4.2.1 The pictorial references that are part of this test method are for illustration purposes and may be used for visual comparisons.  
4.2.2 The diagrams represent an idealized schematic of various growth levels on paint films. They are intended as a representation only, but will serve as a useful guideline to establish amount and type of growth.  
4.2.3 The diagrams represented in Fig. 1 are not derived from a linear scale. The scale is intended to provide for more discrimination at the earlier stages of fungal or algal growth. It is at these levels that greater discernment is necessary.    
4.2.4 Comparisons made on dark colored substrates will be much more difficult, and will therefore require much more care and attention. It must be noted that because it is difficult to distinguish mild fungal or algal growth on the very dark substrates, there may be a tendency to under-rate those specimens.
SCOPE
1.1 Fungal growth, frequently referred to as mildew in the paint industry, causes defacement of paint film exposed outdoors. The visual rating of paint surface disfigurement due to fungal or algal attack is required in order to compare the performance of different coatings.  
1.2 This method of rating mildew evaluation is intended to be used on exterior exposed paint films. This method may be used to rate interior fungal or algal growth, but it should be noted that the growth patterns on interior surfaces are different than exterior due to the lack of weathering influences. It is primarily intended for test specimens, but can also be used for rating mildew growth on larger structures such as entire houses. If this is used for large areas, the project should be broken down into smaller sections.  
1.3 This method is intended for field use for the macro rating of surface disfigurement only. The visual scales are meant to be used by the unaided eye to rate algal, fungal, or dirt disfigurement on larger surface areas such as test panels, siding boards, or entire buildings. Techniques are included for the differentiation of soil and dirt.  
1.4 Fungi will grow on most paint films exposed outdoors that are located in conditions favorable to growth. Test procedures such as Practices D1006, D3456, and G7 are available describing natural exposure tests that can be used to expose paint films, in order to create fungal or algal growth.  
1.5 The pictorial references available for use with this test method provide a numerical basis for rating the degree of fungal or algal growth on paint films.  
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
3.1 Microbial growth is a major cause of discoloration and deterioration of paint films. This guide describes techniques used to distinguish fungi and algae from other surface contaminants.  
3.2 Repainting a surface contaminated with fungi or algae generally causes more rapid infestation of the new paint than repainting a surface from which fungal or algal growth has been removed. In addition, poor adhesion, staining/bleeding, and many other problems may result. This guide describes methods for removing fungal or algal growth prior to repainting.
Note 1: It is extremely important that all steps and notes be read and followed. In particular the washing steps outlined in 5.2.2 and 5.2.4 must be fully carried out, since residual sodium hypochlorite (bleach) can cause many of the same problems, or even loss of coloration after repainting, and residual phosphate detergents can encourage further fungal and algal growth.
Note 2: This procedure is intended for use when recoating the surface. Reports of using this procedure to remove fungi and algae without recoating necessitate inclusion of a warning about the adverse effects of bleach on a coating film. The oxidative effects of bleach can degrade film properties, including particularly the “bleaching” (loss of color) in films containing organic coloring pigments (such as quinacridones, etc.), loss of gloss, chalking, etc. A small patch must be tested prior to use of this procedure for that purpose.
SCOPE
1.1 This guide describes techniques used for determining the presence of fungal or algal growth on paint and related coatings and methods for removal of such growth prior to recoating.  
1.2 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
3.1 Spoilage of paint in the container is often related to the use of contaminated raw materials, water (particularly recycled washwater), vessels, piping, and equipment in the manufacturing plant. There is a need for a simple method to determine the presence or absence of microorganisms in plants that manufacture paints and coatings. Such a determination enables the manufacturer to establish the point of contamination (that is, raw materials or problem housekeeping areas in the plant) to help in solving the spoilage problem.
Note 1: Some contamination in plant areas is to be expected, since microorganisms are ubiquitous and cannot generally be eliminated practically (it is what an in-can preservative is supposed to control). Excessive levels of contamination or contaminated raw materials can exceed the capability of the preservative. If you have excessive contamination in the plant, there are methods for decontamination including steam, preservatives, bleach, etc. These should be discussed with your biocide supplier and used with care. Recovery of spoiled or contaminated products is often not feasible, so an adequate level of the appropriate biocide in conjunction with good plant housekeeping practices are essential. Your biocide supplier can also help here.  
3.2 This test method may be used by persons without basic microbiological training, but some training on aseptic techniques would be recommended.
Note 2: The reliability of the results obtained from this test method is extremely dependent on the techniques employed. Improper techniques can result in a sterile sample appearing to be contaminated, and even worse, a contaminated sample appearing to be sterile (see also 5.1). It is recommended that you consult with your biocide supplier, raw material supplier, or an independent testing laboratory to confirm questionable results.
SCOPE
1.1 This test method covers a procedure for the determination of the microbial condition (contamination or sterility) of raw materials used in the manufacture of paint, and the microbial condition of paint and paint manufacturing areas.  
1.2 The values in SI units are to be regarded as the standard. The values given in parentheses are for information only.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
3.1 An accelerated test for determining the resistance of interior coatings to mold growth is useful in estimating the performance of coatings designed for use in interior environments that promote mold growth and in evaluating compounds that may inhibit such growth and the aggregate levels for their use (see also Note 1).  
3.2 This test method should preferably be used by persons who have had basic microbiological training.
SCOPE
1.1 This test method describes the use of an environmental chamber and operating conditions to evaluate the relative resistance of interior coatings to surface fungal growth in a severe interior environment during a 4-week period.  
1.2 This test method can be used to evaluate the comparative resistance of interior coatings to accelerated mold growth. Performance at a certain rating does not imply any specific period of time for a fungal free coating. However, a better rated coating nearly always performs better in actual end use.
Note 1: This test method is intended for the accelerated evaluation of an interior coatings’ resistance to fungal defacement. Use of this test method for evaluating exterior coatings’ performance has not been validated, nor have the limitations for such use been determined. If this test method is to be used for the testing of an exterior coating system, a precautionary statement regarding interpretation of results as being outside of the scope of this test method must be included in the test report. Any accelerated weathering (leaching, weathering machine exposure, etc.) should be reported and should also bear reference to the fact that it is beyond the current scope of this test method.  
1.3 Temperature and humidity must be effectively controlled within the relatively narrow limits specified in order for the chamber to function reproducibly during the short test period. Severity and rate of mold growth on a film is a function of the moisture content of both the film and the substrate. A relative humidity of >93 % at a temperature of 32.5 ± 1 °C (90 ± 2 °F ) is necessary to initiate and maintain mold growth and for test panels to develop rapidly and maintain an adequate moisture level to support mold growth.  
1.4 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.  
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
4.1 Defacement of paint and coating films by fungal growth (mold, mildew) is a common phenomenon, and defacement by algal growth can also occur under certain conditions. It is generally known that differences in the environment, lighting, temperature, humidity, substrate pH, and other factors in addition to the coating composition affect the susceptibility of a given painted surface. This test method attempts to provide a means to comparatively evaluate different coating formulations for their relative performance under a given set of conditions. It does not imply that a coating that resists growth under these conditions will necessarily resist growth in the actual application. The method is not intended to simulate or replace indoor or outdoor exposure of paint films or related coatings.
Note 1: It is hoped that a ranking of relative performance would be similar to that ranked from outdoor exposures. Paint designated for service in exterior conditions should be pre-conditioned by laboratory accelerated weathering prior to exposure to fungi. All pre-conditioning must be detailed in the final report. This test method however, should not be used as a replacement for exterior exposure (that is, Practice D3456) since many other factors, only a few of which are listed will affect those results.  
4.2 Familiarity with microbiological techniques is required. This test method should not be used by persons without at least basic microbiological training.
SCOPE
1.1 This test method covers an accelerated method for determining the relative resistance of two or more paints or coating films to fungal growth.  
1.2 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
4.1 Spoilage of paint in the container can result in putrefaction, lowered pH, gas formation, and decrease in viscosity. This test method provides a standard procedure for the evaluation of the resistance of emulsion paints to microbial deterioration. The results should enable: (1) the paint manufacturer to select an effective preservative and (2) the supplier of preservatives to evaluate the performance in emulsion paints of competitive and developmental preservatives.  
4.2 This test method should be used preferably by persons who have had basic microbiological training.
Note 1: The reliability of the results obtained from this test method is extremely dependent on the techniques employed. Improper techniques can result in a sterile sample appearing to be contaminated, and even worse, a contaminated sample appearing to be sterile (see also Note 2). It is recommended that you consult with your biocide supplier, raw material supplier, or an independent testing laboratory to confirm questionable results. Formulation and raw materials’ quality may also vary and thereby affect the test results.
SCOPE
1.1 This test method covers the determination of the relative resistance of emulsion paints to attack in the container by microorganisms.  
1.2 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
4.1 Defacement of paint and coating films by algal growth is a common phenomenon under certain conditions. It is generally known that differences in the environment, lighting, temperature, substrate, and other factors in addition to the coating composition affect the susceptibility of a given painted surface. This test method attempts to provide a means to comparatively evaluate different coating formulations for their relative performance under a given set of conditions. It does not imply that a coating that resists growth under these conditions will necessarily resist growth in the actual application.  
4.2 Familiarity with microbiological techniques is required. This test method should not be used by persons without at least basic microbiological training.
SCOPE
1.1 This test method covers an accelerated method for determining the relative resistance of a paint or coating film to algal growth.  
Note 1: It is hoped that a ranking of relative performance would be similar to that ranked from outdoor exposures. However, this test method should not be used as a replacement for exterior exposure since many other factors, only a few of which are listed will affect those results.
Note 2: ASTM weathering standards are no longer referenced in this document, but Practices D822, D4141, D4587, D5031, and D6695 are commonly used.  
1.2 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
4.1 The growth of fungi and algae in and on the surface of paint films represents a major cause of discoloration or disfigurement of painted surfaces. This practice covers the preparation of coatings for testing, their application on substrates, and the arrangement of the coated panels on exterior test fences to determine the degree of microbiological attack that may occur on the surface of the coatings over a period of time. This practice is intended to provide guidelines for, and a discussion of, the various factors critical in selection of exterior coatings resistant to discoloration or disfigurement by algae and fungi.
SCOPE
1.1 This practice provides guidelines for determining the susceptibility of paint films to microbiological attack on exterior exposure. While it is recognized that various organisms may occur on an exposed coating, the specific types of organisms are mainly of academic interest. The degree to which microbiological discoloration occurs is the primary concern.  
1.2 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
4.1 Defacement of paint and coating films by fungal growth (mold, mildew) is a common phenomenon, and defacement by algal growth can also occur under certain conditions. It is generally known that differences in the environment, lighting, temperature, humidity, substrate pH, and other factors in addition to the coating composition affect the susceptibility of a given painted surface. This test method attempts to provide a means to comparatively evaluate different coating formulations for their relative performance under a given set of conditions. It does not imply that a coating that resists growth under these conditions will necessarily resist growth in the actual application. The method is not intended to simulate or replace indoor or outdoor exposure of paint films or related coatings.
Note 1: It is hoped that a ranking of relative performance would be similar to that ranked from outdoor exposures. Paint designated for service in exterior conditions should be pre-conditioned by laboratory accelerated weathering prior to exposure to fungi. All pre-conditioning must be detailed in the final report. This test method however, should not be used as a replacement for exterior exposure (that is, Practice D3456) since many other factors, only a few of which are listed will affect those results.  
4.2 Familiarity with microbiological techniques is required. This test method should not be used by persons without at least basic microbiological training.
SCOPE
1.1 This test method covers an accelerated method for determining the relative resistance of two or more paints or coating films to fungal growth.  
1.2 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
4.1 The growth of fungi in and on the surface of paint films represents a major cause of discoloration or disfigurement of painted surfaces. Because of their dark pigmentation, it is frequently difficult to distinguish fungi from dirt or soil particles.  
4.2 Use of Pictorial Standards:  
4.2.1 The pictorial references that are part of this test method are for illustration purposes and may be used for visual comparisons.  
4.2.2 The diagrams represent an idealized schematic of various growth levels on paint films. they are intended as a representation only, but will serve as a useful guideline to establish amount and type of growth.  
4.2.3 The diagrams represented in Fig. 1 are not derived from a linear scale. The scale is intended to provide for more discrimination at the earlier stages of fungal or algal growth. It is at these levels that greater discernment is necessary.    
4.2.4 Comparisons made on dark colored substrates will be much more difficult, and will therefore require much more care and attention. It must be noted that because it is difficult to distinguish mild fungal or algal growth on the very dark substrates, there may be a tendency to under-rate those specimens.
SCOPE
1.1 Fungal growth, frequently referred to as mildew in the paint industry, causes defacement of paint film exposed outdoors. The visual rating of paint surface disfigurement due to fungal or algal attack is required in order to compare the performance of different coatings.  
1.2 This method of rating mildew evaluation is intended to be used on exterior exposed paint films. This method may be used to rate interior fungal or algal growth, but it should be noted that the growth patterns on interior surfaces are different than exterior due to the lack of weathering influences. It is primarily intended for test specimens, but can also be sued for rating mildew growth on larger structures such as entire houses. If this is used for large areas, the project should be broken down into smaller sections.  
1.3 This method is intended for field use for the macro rating of surface disfigurement only. The visual scales are meant to be used by the unaided eye to rate algal, fungal, or dirt disfigurement on larger surface areas such as test panels, siding boards, or entire buildings. Techniques are included for the differentiation of soil and dirt.  
1.4 Fungi will grow on most paint films exposed outdoors that are located in conditions favorable to growth. Test procedures such as Practices D1006, D3456, and G7 are available describing natural exposure tests that can be used to expose paint films, in order to create fungal or algal growth.  
1.5 The pictorial references available for use with this test method provide a numerical basis for rating the degree of fungal or algal growth on paint films.  
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
5.1 An accelerated test for determining the resistance of interior coated building products to mold growth is useful in estimating the relative performance for use in interior environments under conditions favorable to fungal growth.  
5.2 Static or environmental chambers provide controlled laboratory micro-environment conditions. These chambers are not intended to duplicate room conditions, and care must be taken when interpreting the results. Static chambers are not a substitute for dynamic chambers or field studies.
SCOPE
1.1 This test method covers an environmental chamber and the conditions of operation to evaluate in a 4-week period the relative resistance to mold growth and microbial surface defacement on coated building products designed for interior application using an indirect inoculation method. The apparatus is designed so it can be easily built or obtained by any interested party.  
1.2 This test method can be used to evaluate the comparative resistance of coated building products to accelerated mold growth. Ratings do not imply a specific time period that the coated building product will be free of fungal growth during installation in an interior environment.  
1.3 This test method is not intended for use in the evaluation of public health claims.  
1.4 The test method is intended for the accelerated evaluation of mold growth on a coated building product designed for interior use. This method is not intended for evaluation of surfaces designed for exterior applications or uncoated surfaces. Use of this test method for evaluating exterior performance has not been validated, nor have the limitations for such use been determined.  
1.5 The values stated in either SI units or inch-pound units are to be regarded separately as standard. The values stated in each system may not be exact equivalents; therefore, each system shall be used independently of the other. Combining values from the two systems may result in non-conformance with the standard.  
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
3.1 Microbial growth is a major cause of discoloration and deterioration of paint films. This guide describes techniques used to distinguish fungi and algae from other surface contaminants.  
3.2 Repainting a surface contaminated with fungi or algae generally causes more rapid infestation of the new paint than repainting a surface from which fungal or algal growth has been removed. In addition, poor adhesion, staining/bleeding, and many other problems may result. This guide describes methods for removing fungal or algal growth prior to repainting.
Note 1: It is extremely important that all steps and notes be read and followed. In particular the washing steps outlined in 5.2.2 and 5.2.4 must be fully carried out, since residual sodium hypochlorite (bleach) can cause many of the same problems, or even loss of coloration after repainting, and residual phosphate detergents can encourage further fungal and algal growth.
Note 2: This procedure is intended for use when recoating the surface. Reports of using this procedure to remove fungi and algae without recoating necessitate inclusion of a warning about the adverse effects of bleach on a coating film. The oxidative effects of bleach can degrade film properties, including particularly the “bleaching” (loss of color) in films containing organic coloring pigments (such as quinacridones, etc.), loss of gloss, chalking, etc. A small patch must be tested prior to use of this procedure for that purpose.
SCOPE
1.1 This guide describes techniques used for determining the presence of fungal or algal growth on paint and related coatings and methods for removal of such growth prior to recoating.  
1.2 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
3.1 Spoilage of paint in the container is often related to the use of contaminated raw materials, water (particularly recycled washwater), vessels, piping, and equipment in the manufacturing plant. There is a need for a simple method to determine the presence or absence of microorganisms in plants that manufacture paints and coatings. Such a determination enables the manufacturer to establish the point of contamination (that is, raw materials or problem housekeeping areas in the plant) to help in solving the spoilage problem.
Note 1: Some contamination in plant areas is to be expected, since microorganisms are ubiquitous and cannot generally be eliminated practically (it is what an in-can preservative is supposed to control). Excessive levels of contamination or contaminated raw materials can exceed the capability of the preservative. If you have excessive contamination in the plant, there are methods for decontamination including steam, preservatives, bleach, etc. These should be discussed with your biocide supplier and used with care. Recovery of spoiled or contaminated products is often not feasible, so an adequate level of the appropriate biocide in conjunction with good plant housekeeping practices are essential. Your biocide supplier can also help here.  
3.2 This test method may be used by persons without basic microbiological training, but some training on aseptic techniques would be recommended.
Note 2: The reliability of the results obtained from this test method is extremely dependent on the techniques employed. Improper techniques can result in a sterile sample appearing to be contaminated, and even worse, a contaminated sample appearing to be sterile (see also 5.1). It is recommended that you consult with your biocide supplier, raw material supplier, or an independent testing laboratory to confirm questionable results.
SCOPE
1.1 This test method covers a procedure for the determination of the microbial condition (contamination or sterility) of raw materials used in the manufacture of paint, and the microbial condition of paint and paint manufacturing areas.  
1.2 The values in SI units are to be regarded as the standard. The values given in parentheses are for information only.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.  
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
3.1 An accelerated test for determining the resistance of interior coatings to mold growth is useful in estimating the performance of coatings designed for use in interior environments that promote mold growth and in evaluating compounds that may inhibit such growth and the aggregate levels for their use (see also Note 1).  
3.2 This test method should preferably be used by persons who have had basic microbiological training.
SCOPE
1.1 This test method describes a small environmental chamber and the conditions of operation to evaluate reproducibly in a 4-week period the relative resistance of paint films to surface mold fungi, mildew growth in a severe interior environment. The apparatus is designed so it can be easily built or obtained2 by any interested party and will duplicate results obtained in a large tropical chamber.  
1.2 This test method can be used to evaluate the comparative resistance of interior coating to accelerated mildew growth. Performance at a certain rating does not imply any specific period of time for a fungal free coating. However, a better rated coating nearly always performs better in actual end use.
Note 1: This test method is intended for the accelerated evaluation of an interior coatings’ resistance to fungal defacement. Use of this test method for evaluating exterior coatings’ performance has not been validated, nor have the limitations for such use been determined. If this test method is to be used for the testing of an exterior coating system, a precautionary statement regarding interpretation of results as being outside of the scope of this test method must be included. Any accelerated weathering (leaching, weathering machine exposure, etc.) should be reported and should also bear reference to the fact that it is beyond the current scope of this test method.  
1.3 Temperature and humidity must be effectively controlled within the relatively narrow limits specified in order for the chamber to function reproducibly during the short test period. Severity and rate of mold growth on a film is a function of the moisture content of both the film and the substrate. A relative humidity of 95 ± 3 % at a temperature of 32.5 ± 1°C (90 ± 2°F ) is necessary for test panels to develop rapidly and maintain an adequate moisture level to support mold growth.  
1.4 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.  
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SIGNIFICANCE AND USE
4.1 Spoilage of paint in the container can result in putrefaction, lowered pH, gas formation, and decrease in viscosity. This test method provides a standard procedure for the evaluation of the resistance of emulsion paints to microbial deterioration. The results should enable: (1) the paint manufacturer to select an effective preservative and (2) the supplier of preservatives to evaluate the performance in emulsion paints of competitive and developmental preservatives.  
4.2 This test method should be used preferably by persons who have had basic microbiological training.
Note 1: The reliability of the results obtained from this test method is extremely dependent on the techniques employed. Improper techniques can result in a sterile sample appearing to be contaminated, and even worse, a contaminated sample appearing to be sterile (see also Note 2). It is recommended that you consult with your biocide supplier, raw material supplier, or an independent testing laboratory to confirm questionable results. Formulation and raw materials’ quality may also vary and thereby affect the test results.
SCOPE
1.1 This test method covers the determination of the relative resistance of emulsion paints to attack in the container by microorganisms.  
1.2 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SIGNIFICANCE AND USE
4.1 The growth of fungi in and on the surface of paint films represents a major cause of discoloration or disfigurement of painted surfaces. Because of their dark pigmentation, it is frequently difficult to distinguish fungi from dirt or soil particles.  
4.2 Use of Pictorial Standards:  
4.2.1 The pictorial references that are part of this test method are for illustration purposes and may be used for visual comparisons.  
4.2.2 The diagrams represent an idealized schematic of various growth levels on paint films. they are intended as a representation only, but will serve as a useful guideline to establish amount and type of growth.  
4.2.3 The diagrams represented in Fig. 1 are not derived from a linear scale. The scale is intended to provide for more discrimination at the earlier stages of fungal or algal growth. It is at these levels that greater discernment is necessary.
4.2.4 Comparisons made on dark colored substrates will be much more difficult, and will therefore require much more care and attention. It must be noted that because it is difficult to distinguish mild fungal or algal growth on the very dark substrates, there may be a tendency to under-rate those specimens.
SCOPE
1.1 Fungal growth, frequently referred to as mildew in the paint industry, causes defacement of paint film exposed outdoors. The visual rating of paint surface disfigurement due to fungal or algal attack is required in order to compare the performance of different coatings.  
1.2 This method of rating mildew evaluation is intended to be used on exterior exposed paint films. This method may be used to rate interior fungal or algal growth, but it should be noted that the growth patterns on interior surfaces are different than exterior due to the lack of weathering influences. It is primarily intended for test specimens, but can also be sued for rating mildew growth on larger structures such as entire houses. If this is used for large areas, the project should be broken down into smaller sections.  
1.3 This method is intended for field use for the macro rating of surface disfigurement only. The visual scales are meant to be used by the unaided eye to rate algal, fungal, or dirt disfigurement on larger surface areas such as test panels, siding boards, or entire buildings. Techniques are included for the differentiation of soil and dirt.  
1.4 Fungi will grow on most paint films exposed outdoors that are located in conditions favorable to growth. Test procedures such as Practices D1006, D3456, and G7 are available describing natural exposure tests that can be used to expose paint films, in order to create fungal or algal growth.  
1.5 The pictorial references available for use with this test method provide a numerical basis for rating the degree of fungal or algal growth on paint films.  
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SIGNIFICANCE AND USE
4.1 Defacement of paint and coating films by algal growth is a common phenomenon under certain conditions. It is generally known that differences in the environment, lighting, temperature, substrate, and other factors in addition to the coating composition affect the susceptibility of a given painted surface. This test method attempts to provide a means to comparatively evaluate different coating formulations for their relative performance under a given set of conditions. It does not imply that a coating that resists growth under these conditions will necessarily resist growth in the actual application (see Note 1).  
4.2 Familiarity with microbiological techniques is required. This test method should not be used by persons without at least basic microbiological training.
SCOPE
1.1 This test method covers an accelerated method for determining the relative resistance of a paint or coating film to algal growth. Note 1—It is hoped that a ranking of relative performance would be similar to that ranked from outdoor exposures. However, this test method should not be used as a replacement for exterior exposure since many other factors, only a few of which are listed will affect those results.  
1.2 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SIGNIFICANCE AND USE
5.1 An accelerated test for determining the resistance of interior coated building products to mold growth is useful in estimating the relative performance for use in interior environments under conditions favorable to fungal growth.  
5.2 Static or environmental chambers provide controlled laboratory micro-environment conditions. These chambers are not intended to duplicate room conditions, and care must be taken when interpreting the results. Static chambers are not a substitute for dynamic chambers or field studies.
SCOPE
1.1 This test method covers an environmental chamber and the conditions of operation to evaluate in a 4-week period the relative resistance to mold growth and microbial surface defacement on coated building products designed for interior application using an indirect inoculation method. The apparatus is designed so it can be easily built or obtained by any interested party.  
1.2 This test method can be used to evaluate the comparative resistance of coated building products to accelerated mold growth. Ratings do not imply a specific time period that the coated building product will be free of fungal growth during installation in an interior environment.  
1.3 This test method is not intended for use in the evaluation of public health claims.  
1.4 The test method is intended for the accelerated evaluation of mold growth on a coated building product designed for interior use. This method is not intended for evaluation of surfaces designed for exterior applications or uncoated surfaces. Use of this test method for evaluating exterior performance has not been validated, nor have the limitations for such use been determined.  
1.5 The values stated in either SI units or inch-pound units are to be regarded separately as standard. The values stated in each system may not be exact equivalents; therefore, each system shall be used independently of the other. Combining values from the two systems may result in non-conformance with the standard.  
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SIGNIFICANCE AND USE
3.1 Microbial growth is a major cause of discoloration and deterioration of paint films. This guide describes techniques used to distinguish fungi and algae from other surface contaminants.  
3.2 Repainting a surface contaminated with fungi or algae generally causes more rapid infestation of the new paint than repainting a surface from which fungal or algal growth has been removed. In addition, poor adhesion, staining/bleeding, and many other problems may result. This guide describes methods for removing fungal or algal growth prior to repainting.Note 1—It is extremely important that all steps and notes be read and followed. In particular the washing steps outlined in 5.2.2 and 5.2.4 must be fully carried out, since residual sodium hypochlorite (bleach) can cause many of the same problems, or even loss of coloration after repainting, and residual phosphate detergents can encourage further fungal and algal growth.Note 2—This procedure is intended for use when recoating the surface. Reports of using this procedure to remove fungi and algae without recoating necessitate inclusion of a warning about the adverse effects of bleach on a coating film. The oxidative effects of bleach can degrade film properties, including particularly the “bleaching” (loss of color) in films containing organic coloring pigments (such as quinacridones, etc.), loss of gloss, chalking, etc. A small patch must be tested prior to use of this procedure for that purpose.
SCOPE
1.1 This guide describes techniques used for determining the presence of fungal or algal growth on paint and related coatings and methods for removal of such growth prior to recoating.  
1.2 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SIGNIFICANCE AND USE
4.1 The growth of fungi and algae in and on the surface of paint films represents a major cause of discoloration or disfigurement of painted surfaces. This practice covers the preparation of coatings for testing, their application on substrates, and the arrangement of the coated panels on exterior test fences to determine the degree of microbiological attack that may occur on the surface of the coatings over a period of time. This practice is intended to provide guidelines for, and a discussion of, the various factors critical in selection of exterior coatings resistant to discoloration or disfigurement by algae and fungi.
SCOPE
1.1 This practice provides guidelines for determining the susceptibility of paint films to microbiological attack on exterior exposure. While it is recognized that various organisms may occur on an exposed coating, the specific types of organisms are mainly of academic interest. The degree to which microbiological discoloration occurs is the primary concern.  
1.2 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SIGNIFICANCE AND USE
3.1 Spoilage of paint in the container is often related to the use of contaminated raw materials, water (particularly recycled washwater), vessels, piping, and equipment in the manufacturing plant. There is a need for a simple method to determine the presence or absence of microorganisms in plants that manufacture paints and coatings. Such a determination enables the manufacturer to establish the point of contamination (that is, raw materials or problem housekeeping areas in the plant) to help in solving the spoilage problem.Note 1—Some contamination in plant areas is to be expected, since microorganisms are ubiquitous and cannot generally be eliminated practically (it is what an in-can preservative is supposed to control). Excessive levels of contamination or contaminated raw materials can exceed the capability of the preservative. If you have excessive contamination in the plant, there are methods for decontamination including steam, preservatives, bleach, etc. These should be discussed with your biocide supplier and used with care. Recovery of spoiled or contaminated products is often not feasible, so an adequate level of the appropriate biocide in conjunction with good plant housekeeping practices are essential. Your biocide supplier can also help here.  
3.2 This test method may be used by persons without basic microbiological training, but some training on aseptic techniques would be recommended.Note 2—The reliability of the results obtained from this test method is extremely dependent on the techniques employed. Improper techniques can result in a sterile sample appearing to be contaminated, and even worse, a contaminated sample appearing to be sterile (see also 5.1). It is recommended that you consult with your biocide supplier, raw material supplier, or an independent testing laboratory to confirm questionable results.
SCOPE
1.1 This test method covers a procedure for the determination of the microbial condition (contamination or sterility) of raw materials used in the manufacture of paint, and the microbial condition of paint and paint manufacturing areas.  
1.2 The values in SI units are to be regarded as the standard. The values given in parentheses are for information only.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SIGNIFICANCE AND USE
4.1 Spoilage of paint in the container can result in putrefaction, lowered pH, gas formation, and decrease in viscosity. This test method provides a standard procedure for the evaluation of the resistance of emulsion paints to microbial deterioration. The results should enable: (1) the paint manufacturer to select an effective preservative and (2) the supplier of preservatives to evaluate the performance in emulsion paints of competitive and developmental preservatives.  
4.2 This test method should be used preferably by persons who have had basic microbiological training.Note 1—The reliability of the results obtained from this test method is extremely dependent on the techniques employed. Improper techniques can result in a sterile sample appearing to be contaminated, and even worse, a contaminated sample appearing to be sterile (see also Note 2). It is recommended that you consult with your biocide supplier, raw material supplier, or an independent testing laboratory to confirm questionable results. Formulation and raw materials' quality may also vary and thereby affect the test results.
SCOPE
1.1 This test method covers the determination of the relative resistance of emulsion paints to attack in the container by microorganisms.  
1.2 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SIGNIFICANCE AND USE
An accelerated test for determining the resistance of interior coatings to mold growth is useful in estimating the performance of coatings designed for use in interior environments that promote mold growth and in evaluating compounds that may inhibit such growth and the aggregate levels for their use (see also Note 1).
This test method should preferably be used by persons who have had basic microbiological training.
FIG. 1 Environmental Cabinet Assembly
SCOPE
1.1 This test method describes a small environmental chamber and the conditions of operation to evaluate reproducibly in a 4-week period the relative resistance of paint films to surface mold fungi, mildew growth in a severe interior environment. The apparatus is designed so it can be easily built or obtained by any interested party and will duplicate results obtained in a large tropical chamber.
1.2 This test method can be used to evaluate the comparative resistance of interior coating to accelerated mildew growth. Performance at a certain rating does not imply any specific period of time for a fungal free coating. However, a better rated coating nearly always performs better in actual end use.
Note 1—This test method is intended for the accelerated evaluation of an interior coatings' resistance to fungal defacement. Use of this test method for evaluating exterior coatings' performance has not been validated, nor have the limitations for such use been determined. If this test method is to be used for the testing of an exterior coating system, a precautionary statement regarding interpretation of results as being outside of the scope of this test method must be included. Any accelerated weathering (leaching, weathering machine exposure, etc.) should be reported and should also bear reference to the fact that it is beyond the current scope of this test method.
1.3 Temperature and humidity must be effectively controlled within the relatively narrow limits specified in order for the chamber to function reproducibly during the short test period. Severity and rate of mold growth on a film is a function of the moisture content of both the film and the substrate. A relative humidity of 95 ± 3 % at a temperature of 32.5 ± 1°C (90 ± 2°F ) is necessary for test panels to develop rapidly and maintain an adequate moisture level to support mold growth.
1.4 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SIGNIFICANCE AND USE
3.1 An accelerated test for determining the resistance of interior coatings to mold growth is useful in estimating the performance of coatings designed for use in interior environments that promote mold growth and in evaluating compounds that may inhibit such growth and the aggregate levels for their use (see also Note 1).  
3.2 This test method should preferably be used by persons who have had basic microbiological training.
SCOPE
1.1 This test method describes a small environmental chamber and the conditions of operation to evaluate reproducibly in a 4-week period the relative resistance of paint films to surface mold fungi, mildew growth in a severe interior environment. The apparatus is designed so it can be easily built or obtained2 by any interested party and will duplicate results obtained in a large tropical chamber.  
1.2 This test method can be used to evaluate the comparative resistance of interior coating to accelerated mildew growth. Performance at a certain rating does not imply any specific period of time for a fungal free coating. However, a better rated coating nearly always performs better in actual end use.
Note 1: This test method is intended for the accelerated evaluation of an interior coatings’ resistance to fungal defacement. Use of this test method for evaluating exterior coatings’ performance has not been validated, nor have the limitations for such use been determined. If this test method is to be used for the testing of an exterior coating system, a precautionary statement regarding interpretation of results as being outside of the scope of this test method must be included. Any accelerated weathering (leaching, weathering machine exposure, etc.) should be reported and should also bear reference to the fact that it is beyond the current scope of this test method.  
1.3 Temperature and humidity must be effectively controlled within the relatively narrow limits specified in order for the chamber to function reproducibly during the short test period. Severity and rate of mold growth on a film is a function of the moisture content of both the film and the substrate. A relative humidity of 95 ± 3 % at a temperature of 32.5 ± 1°C (90 ± 2°F ) is necessary for test panels to develop rapidly and maintain an adequate moisture level to support mold growth.  
1.4 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.  
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SIGNIFICANCE AND USE
4.1 Defacement of paint and coating films by fungal growth (mold, mildew) is a common phenomenon, and defacement by algal growth can also occur under certain conditions. It is generally known that differences in the environment, lighting, temperature, humidity, substrate pH, and other factors in addition to the coating composition affect the susceptibility of a given painted surface. This test method attempts to provide a means to comparatively evaluate different coating formulations for their relative performance under a given set of conditions. It does not imply that a coating that resists growth under these conditions will necessarily resist growth in the actual application.Note 1—It is hoped that a ranking of relative performance would be similar to that ranked from outdoor exposures. However, this test method should not be used as a replacement for exterior exposure (that is, Practice D3456) since many other factors, only a few of which are listed will affect those results.Note 2—Several companies have reported reasonable correlation of results from this test with actual use when testing film-forming, pigmented coatings. Round-robin testing of this test method versus exterior exposure is planned.  
4.2 Familiarity with microbiological techniques is required. This test method should not be used by persons without at least basic microbiological training.
SCOPE
1.1 This test method covers an accelerated method for determining the relative resistance of two or more paints or coating films to fungal growth.  
1.2 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SIGNIFICANCE AND USE
Defacement of paint and coating films by fungal growth (mold, mildew) is a common phenomenon, and defacement by algal growth can also occur under certain conditions. It is generally known that differences in the environment, lighting, temperature, humidity, substrate pH, and other factors in addition to the coating composition affect the susceptibility of a given painted surface. This test method attempts to provide a means to comparatively evaluate different coating formulations for their relative performance under a given set of conditions. It does not imply that a coating that resists growth under these conditions will necessarily resist growth in the actual application.
Note 1—It is hoped that a ranking of relative performance would be similar to that ranked from outdoor exposures. However, this test method should not be used as a replacement for exterior exposure (that is, Practice D3456) since many other factors, only a few of which are listed will affect those results.
Note 2—Several companies have reported reasonable correlation of results from this test with actual use when testing film-forming, pigmented coatings. Round-robin testing of this test method versus exterior exposure is planned.
Familiarity with microbiological techniques is required. This test method should not be used by persons without at least basic microbiological training.
SCOPE
1.1 This test method covers an accelerated method for determining the relative resistance of two or more paints or coating films to fungal growth.
1.2 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SIGNIFICANCE AND USE
Microbial growth is a major cause of discoloration and deterioration of paint films. This guide describes techniques used to distinguish fungi and algae from other surface contaminants.
Repainting a surface contaminated with fungi or algae generally causes more rapid infestation of the new paint than repainting a surface from which fungal or algal growth has been removed. In addition, poor adhesion, staining/bleeding, and many other problems may result. This guide describes methods for removing fungal or algal growth prior to repainting.
Note 1—It is extremely important that all steps and notes be read and followed. In particular the washing steps outlined in 5.2.2 and 5.2.4 must be fully carried out, since residual sodium hypochlorite (bleach) can cause many of the same problems, or even loss of coloration after repainting, and residual phosphate detergents can encourage further fungal and algal growth.
Note 2—This procedure is intended for use when recoating the surface. Reports of using this procedure to remove fungi and algae without recoating necessitate inclusion of a warning about the adverse effects of bleach on a coating film. The oxidative effects of bleach can degrade film properties, including particularly the “bleaching” (loss of color) in films containing organic coloring pigments (such as quinacridones, etc.), loss of gloss, chalking, etc. A small patch must be tested prior to use of this procedure for that purpose.
SCOPE
1.1 This guide describes techniques used for determining the presence of fungal or algal growth on paint and related coatings and methods for removal of such growth prior to recoating.
1.2 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SIGNIFICANCE AND USE
Defacement of paint and coating films by algal growth is a common phenomenon under certain conditions. It is generally known that differences in the environment, lighting, temperature, substrate, and other factors in addition to the coating composition affect the susceptibility of a given painted surface. This test method attempts to provide a means to comparatively evaluate different coating formulations for their relative performance under a given set of conditions. It does not imply that a coating that resists growth under these conditions will necessarily resist growth in the actual application (see Note 1).
Familiarity with microbiological techniques is required. This test method should not be used by persons without at least basic microbiological training.
SCOPE
1.1 This test method covers an accelerated method for determining the relative resistance of a paint or coating film to algal growth.
Note 1—It is hoped that a ranking of relative performance would be similar to that ranked from outdoor exposures. However, this test method should not be used as a replacement for exterior exposure since many other factors, only a few of which are listed will affect those results.  
1.2 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SIGNIFICANCE AND USE
The growth of fungi in and on the surface of paint films represents a major cause of discoloration or disfigurement of painted surfaces. Because of their dark pigmentation, it is frequently difficult to distinguish fungi from dirt or soil particles.
Use of Pictorial Standards:
The pictorial references that are part of this test method are for illustration purposes and may be used for visual comparisons.
The diagrams represent an idealized schematic of various growth levels on paint films. they are intended as a representation only, but will serve as a useful guideline to establish amount and type of growth.
The diagrams represented in Fig. 1 are not derived from a linear scale. The scale is intended to provide for more discrimination at the earlier stages of fungal or algal growth. It is at these levels that greater discernment is necessary.
Comparisons made on dark colored substrates will be much more difficult, and will therefore require much more care and attention. It must be noted that because it is difficult to distinguish mild fungal or algal growth on the very dark substrates, there may be a tendency to under-rate those specimens.
SCOPE
1.1 Fungal growth, frequently referred to as mildew in the paint industry, causes defacement of paint film exposed outdoors. The visual rating of paint surface disfigurement due to fungal or algal attack is required in order to compare the performance of different coatings.
1.2 This method of rating mildew evaluation is intended to be used on exterior exposed paint films. This method may be used to rate interior fungal or algal growth, but it should be noted that the growth patterns on interior surfaces are different than exterior due to the lack of weathering influences. It is primarily intended for test specimens, but can also be sued for rating mildew growth on larger structures such as entire houses. If this is used for large areas, the project should be broken down into smaller sections.
1.3 This method is intended for field use for the macro rating of surface disfigurement only. The visual scales are meant to be used by the unaided eye to rate algal, fungal, or dirt disfigurement on larger surface areas such as test panels, siding boards, or entire buildings. Techniques are included for the differentiation of soil and dirt.
1.4 Fungi will grow on most paint films exposed outdoors that are located in conditions favorable to growth. Test procedures such as Practices D1006, D3456, and G7 are available describing natural exposure tests that can be used to expose paint films, in order to create fungal or algal growth.
1.5 The pictorial references available for use with this test method provide a numerical basis for rating the degree of fungal or algal growth on paint films.
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SIGNIFICANCE AND USE
The growth of fungi in and on the surface of paint films represents a major cause of discoloration or disfigurement of painted surfaces. Because of their dark pigmentation, it is frequently difficult to distinguish fungi from dirt or soil particles.
Use of Pictorial Standards:
The pictorial references that are part of this test method are for illustration purposes and may be used for visual comparisons.
The diagrams represent an idealized schematic of various growth levels on paint films. they are intended as a representation only, but will serve as a useful guideline to establish amount and type of growth.
The diagrams represented in Fig. 1 are not derived from a linear scale. The scale is intended to provide for more discrimination at the earlier stages of fungal or algal growth. It is at these levels that greater discernment is necessary.
Comparisons made on dark colored substrates will be much more difficult, and will therefore require much more care and attention. It must be noted that because it is difficult to distinguish mild fungal or algal growth on the very dark substrates, there may be a tendency to under-rate those specimens.
       (a) FIG. 1 Early Stages of Fungal Growth
   (b) FIG. 1Early Stages of Fungal Growth (continued)
   (c) FIG. 1Early Stages of Fungal Growth (continued)
SCOPE
1.1 Fungal growth, frequently referred to as mildew in the paint industry, causes defacement of paint film exposed outdoors. The visual rating of paint surface disfigurement due to fungal or algal attack is required in order to compare the performance of different coatings.
1.2 This method of rating mildew evaluation is intended to be used on exterior exposed paint films. This method may be used to rate interior fungal or algal growth, but it should be noted that the growth patterns on interior surfaces are different than exterior due to the lack of weathering influences. It is primarily intended for test specimens, but can also be sued for rating mildew growth on larger structures such as entire houses. If this is used for large areas, the project should be broken down into smaller sections.
1.3 This method is intended for field use for the macro rating of surface disfigurement only. The visual scales are meant to be used by the unaided eye to rate algal, fungal, or dirt disfigurement on larger surface areas such as test panels, siding boards, or entire buildings. Techniques are included for the differentiation of soil and dirt.
1.4 Fungi will grow on most paint films exposed outdoors that are located in conditions favorable to growth. Test procedures such as Practices D 1006, D 3456, and G 7 are available describing natural exposure tests that can be used to expose paint films, in order to create fungal or algal growth.
1.5 The pictorial references available for use with this test method provide a numerical basis for rating the degree of fungal or algal growth on paint films.
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SIGNIFICANCE AND USE
The growth of fungi and algae in and on the surface of paint films represents a major cause of discoloration or disfigurement of painted surfaces. This practice covers the preparation of coatings for testing, their application on substrates, and the arrangement of the coated panels on exterior test fences to determine the degree of microbiological attack that may occur on the surface of the coatings over a period of time. This practice is intended to provide guidelines for, and a discussion of, the various factors critical in selection of exterior coatings resistant to discoloration or disfigurement by algae and fungi.
SCOPE
1.1 This practice provides guidelines for determining the susceptibility of paint films to microbiological attack on exterior exposure. While it is recognized that various organisms may occur on an exposed coating, the specific types of organisms are mainly of academic interest. The degree to which microbiological discoloration occurs is the primary concern.
1.2 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SIGNIFICANCE AND USE
Spoilage of paint in the container is often related to the use of contaminated raw materials, water (particularly recycled washwater), vessels, piping, and equipment in the manufacturing plant. There is a need for a simple method to determine the presence or absence of microorganisms in plants that manufacture paints and coatings. Such a determination enables the manufacturer to establish the point of contamination (that is, raw materials or problem housekeeping areas in the plant) to help in solving the spoilage problem.
Note 1—Some contamination in plant areas is to be expected, since microorganisms are ubiquitous and cannot generally be eliminated practically (it is what an in-can preservative is supposed to control). Excessive levels of contamination or contaminated raw materials can exceed the capability of the preservative. If you have excessive contamination in the plant, there are methods for decontamination including steam, preservatives, bleach, etc. These should be discussed with your biocide supplier and used with care. Recovery of spoiled or contaminated products is often not feasible, so an adequate level of the appropriate biocide in conjunction with good plant housekeeping practices are essential. Your biocide supplier can also help here.
This test method may be used by persons without basic microbiological training, but some training on aseptic techniques would be recommended.
Note 2—The reliability of the results obtained from this test method is extremely dependent on the techniques employed. Improper techniques can result in a sterile sample appearing to be contaminated, and even worse, a contaminated sample appearing to be sterile (see also 5.1). It is recommended that you consult with your biocide supplier, raw material supplier, or an independent testing laboratory to confirm questionable results.
SCOPE
1.1 This test method covers a procedure for the determination of the microbial condition (contamination or sterility) of raw materials used in the manufacture of paint, and the microbial condition of paint and paint manufacturing areas.
1.2 The values in SI units are to be regarded as the standard. The values given in parentheses are for information only.
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SCOPE
1.1 This test method covers the determination of the relative resistance of emulsion paints to attack in the container by microorganisms.
1.2 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.
This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SIGNIFICANCE AND USE
An accelerated test for determining the resistance of interior coatings to mold growth is useful in estimating the performance of coatings designed for use in interior environments that promote mold growth and in evaluating compounds that may inhibit such growth and the aggregate levels for their use (see also Note 1).
This test method should preferably be used by persons who have had basic microbiological training.
FIG. 1 Environmental Cabinet Assembly
SCOPE
1.1 This test method describes a small environmental chamber and the conditions of operation to evaluate reproducibly in a 4-week period the relative resistance of paint films to surface mold fungi, mildew growth in a severe interior environment. The apparatus is designed so it can be easily built or obtained by any interested party and will duplicate results obtained in a large tropical chamber.
1.2 This test method can be used to evaluate the comparative resistance of interior coating to accelerated mildew growth. Performance at a certain rating (in accordance with Test Method D3274) does not imply any specific period of time for a fungal free coating. However, a better rated coating nearly always performs better in actual end use.
Note 1--This test method is intended for the accelerated evaluation of an interior coatings' resistance to fungal defacement. Use of this test method for evaluating exterior coatings' performance has not been validated, nor have the limitations for such use been determined. Should this test method be used for the testing of an exterior coating system, a precautionary statement regarding interpretation of results as being outside of the scope of this test method must be included. Any accelerated weathering (leaching, weathering machine exposure, etc.,) should be reported and should also bear reference to the fact that it is beyond the current scope of this test method.
1.3 Temperature and humidity must be effectively controlled within the relatively narrow limits specified in order for the chamber to function reproducibly during the short test period. Severity and rate of mold growth on a film is a function of the moisture content of both the film and the substrate. A relative humidity of 95 to 98 % at a temperature of 32.5+ 1oC (90+ 2oF ) is necessary for test panels to develop rapidly and maintain an adequate moisture level to support mold growth.
1.4 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.
This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SIGNIFICANCE AND USE
Defacement of paint and coating films by fungal growth (mold, mildew) is a common phenomenon, and defacement by algal growth can also occur under certain conditions. It is generally known that differences in the environment, lighting, temperature, humidity, substrate pH, and other factors in addition to the coating composition affect the susceptibility of a given painted surface. This test method attempts to provide a means to comparatively evaluate different coating formulations for their relative performance under a given set of conditions. It does not imply that a coating that resists growth under these conditions will necessarily resist growth in the actual application.  
Note 1—It is hoped that a ranking of relative performance would be similar to that ranked from outdoor exposures. However, this test method should not be used as a replacement for exterior exposure (that is, Practice D 3456) since many other factors, only a few of which are listed will affect those results.
Note 2—Several companies have reported reasonable correlation of results from this test with actual use when testing film-forming, pigmented coatings. Round-robin testing of this test method versus exterior exposure is planned.
Familiarity with microbiological techniques is required. This test method should not be used by persons without at least basic microbiological training.
SCOPE
1.1 This test method covers an accelerated method for determining the relative resistance of two or more paints or coating films to fungal growth.
1.2 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.
This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SIGNIFICANCE AND USE
Microbial growth is a major cause of discoloration and deterioration of paint films. This guide describes techniques used to distinguish fungi and algae from other surface contaminants.
Repainting a surface contaminated with fungi or algae generally causes more rapid infestation of the new paint than repainting a surface from which fungal or algal growth has been removed. In addition, poor adhesion, staining/bleeding, and many other problems may result. This guide describes methods for removing fungal or algal growth prior to repainting.
Note 1—It is extremely important that all steps and notes be read and followed. In particular the washing steps outlined in 5.2.2 and 5.2.4 must be fully carried out, since residual sodium hypochlorite (bleach) can cause many of the same problems, or even loss of coloration after repainting, and residual phosphate detergents can encourage further fungal and algal growth.
Note 2—This procedure is intended for use when recoating the surface. Reports of using this procedure to remove fungi and algae without recoating necessitate inclusion of a warning about the adverse effects of bleach on a coating film. The oxidative effects of bleach can degrade film properties, including particularly the “bleaching” (loss of color) in films containing organic coloring pigments (such as quinacridones, etc.), loss of gloss, chalking, etc. A small patch must be tested prior to use of this procedure for that purpose.
SCOPE
1.1 This guide describes techniques used for determining the presence of fungal or algal growth on paint and related coatings and methods for removal of such growth prior to recoating.
1.2 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SCOPE
1.1 This test method covers an accelerated method for determining the relative resistance of two or more paints or coating films to fungal growth.
1.2 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.
1.3  This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SCOPE
1.1 This test method covers an accelerated method for determining the relative resistance of two or more paints or coating films to fungal growth.
1.2 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.
1.3  This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SCOPE
1.1 This test method describes a small environmental chamber and the conditions of operation to evaluate reproducibly in a 4-week period the relative resistance of paint films to surface mold fungi, mildew growth in a severe interior environment. The apparatus is designed so it can be easily built or obtained by any interested party and will duplicate results obtained in a large tropical chamber.
1.2 This test method can be used to evaluate the comparative resistance of interior coating to accelerated mildew growth. Performance at a certain rating (in accordance with Test Method D3274) does not imply any specific period of time for a fungal free coating. However, a better rated coating nearly always performs better in actual end use.
Note 1--This test method is intended for the accelerated evaluation of an interior coatings' resistance to fungal defacement. Use of this test method for evaluating exterior coatings' performance has not been validated, nor have the limitations for such use been determined. Should this test method be used for the testing of an exterior coating system, a precautionary statement regarding interpretation of results as being outside of the scope of this test method must be included. Any accelerated weathering (leaching, weathering machine exposure, etc.,) should be reported and should also bear reference to the fact that it is beyond the current scope of this test method.
1.3 Temperature and humidity must be effectively controlled within the relatively narrow limits specified in order for the chamber to function reproducibly during the short test period. Severity and rate of mold growth on a film is a function of the moisture content of both the film and the substrate. A relative humidity of 95 to 98 % at a temperature of 32.5+ 1oC (90+ 2oF ) is necessary for test panels to develop rapidly and maintain an adequate moisture level to support mold growth.
1.4 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.
This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SCOPE
1.1 This test method covers the determination of the relative resistance of emulsion paints to attack in the container by microorganisms.
1.2 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SCOPE
1.1 This guide describes techniques used for determining the presence of fungal or algal growth on paint and related coatings and methods for removal of such growth prior to recoating.  
1.2 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SCOPE
1.1 This test method covers an accelerated method for determining the relative resistance of a paint or coating film to algal growth.
1.2 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SIGNIFICANCE AND USE
Spoilage of paint in the container is often related to the use of contaminated raw materials, water (particularly recycled washwater), vessels, piping, and equipment in the manufacturing plant. There is a need for a simple method to determine the presence or absence of microorganisms in plants that manufacture paints and coatings. Such a determination enables the manufacturer to establish the point of contamination (that is, raw materials or problem housekeeping areas in the plant) to help in solving the spoilage problem.
Note 1—Some contamination in plant areas is to be expected, since microorganisms are ubiquitous and cannot generally be eliminated practically (it is what an in-can preservative is supposed to control). Excessive levels of contamination or contaminated raw materials can exceed the capability of the preservative. If you have excessive contamination in the plant, there are methods for decontamination including steam, preservatives, bleach, etc. These should be discussed with your biocide supplier and used with care. Recovery of spoiled or contaminated products is often not feasible, so an adequate level of the appropriate biocide in conjunction with good plant housekeeping practices are essential. Your biocide supplier can also help here.
This test method may be used by persons without basic microbiological training, but some training on aseptic techniques would be recommended.
Note 2—The reliability of the results obtained from this test method is extremely dependent on the techniques employed. Improper techniques can result in a sterile sample appearing to be contaminated, and even worse, a contaminated sample appearing to be sterile (see also 5.1). It is recommended that you consult with your biocide supplier, raw material supplier, or an independent testing laboratory to confirm questionable results.
SCOPE
1.1 This test method covers a procedure for the determination of the microbial condition (contamination or sterility) of raw materials used in the manufacture of paint, and the microbial condition of paint and paint manufacturing areas.  
1.2 The values in SI units are to be regarded as the standard. The values given in parentheses are for information only.  
1.3  This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SIGNIFICANCE AND USE
Defacement of paint and coating films by algal growth is a common phenomenon under certain conditions. It is generally known that differences in the environment, lighting, temperature, substrate, and other factors in addition to the coating composition affect the susceptibility of a given painted surface. This test method attempts to provide a means to comparatively evaluate different coating formulations for their relative performance under a given set of conditions. It does not imply that a coating that resists growth under these conditions will necessarily resist growth in the actual application (see Note 1).
Familiarity with microbiological techniques is required. This test method should not be used by persons without at least basic microbiological training.
SCOPE
1.1 This test method covers an accelerated method for determining the relative resistance of a paint or coating film to algal growth.
Note 1—It is hoped that a ranking of relative performance would be similar to that ranked from outdoor exposures. However, this test method should not be used as a replacement for exterior exposure since many other factors, only a few of which are listed will affect those results.
1.2 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SCOPE
1.1 This test method covers a procedure for the determination of the microbial condition (contamination or sterility) of raw materials used in the manufacture of paint, and the microbial condition of paint and paint manufacturing areas.  
1.2 The values in SI units are to be regarded as the standard. The values given in parentheses are for information only.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SCOPE
1.1 This practice provides guidelines for determining the susceptibility of paint films to microbiological attack on exterior exposure. While it is recognized that various organisms may occur on an exposed coating, the specific types of organisms are mainly of academic interest. The degree to which microbiological discoloration occurs is the primary concern.
1.2 This standard does not purport to address all of the safety problems, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SIGNIFICANCE AND USE
The growth of fungi and algae in and on the surface of paint films represents a major cause of discoloration or disfigurement of painted surfaces. Because of their dark pigmentation, it is frequently difficult to distinguish fungi and algae from soil or dirt particles. The use of magnification of as much as 100× affords easy distinguishability among fungal spores, fungal mycelia, algae, and dirt and is recommended in cases where such difficulty is apparent.
Dirt accumulation observed on specimens can occur more readily with coatings formulated at lower pigment volume concentration or those containing softer resins.
Use of Photographic Standards:
3  
4.3.1 The photographic reference standards that are part of this test method are for illustration purposes and should be used for visual comparisons.
4.3.2 The photographic standards illustrated in Fig. 4 rate the disfigurement of paint films from 0 to 8. A rating of 10 would indicate a film totally absent of disfigurement by particulate matter.
4.3.3 The ratings used in this standard more closely match a nonlinear scale.
FIG. 4 Disfigurement Ratings
SCOPE
1.1 The photographic reference standards available for use with this test method provide a numerical basis for rating the degree of fungal and algal growth or soil and dirt accumulation on paint films.
1.2 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SCOPE
1.1 The photographic reference standards available for use with this test method provide a numerical basis for rating the degree of fungal and algal growth or soil and dirt accumulation on paint films.
1.2 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SIGNIFICANCE AND USE
The growth of fungi and algae in and on the surface of paint films represents a major cause of discoloration or disfigurement of painted surfaces. This practice covers the preparation of coatings for testing, their application on substrates, and the arrangement of the coated panels on exterior test fences to determine the degree of microbiological attack that may occur on the surface of the coatings over a period of time. This practice is intended to provide guidelines for, and a discussion of, the various factors critical in selection of exterior coatings resistant to discoloration or disfigurement by algae and fungi.
SCOPE
1.1 This practice provides guidelines for determining the susceptibility of paint films to microbiological attack on exterior exposure. While it is recognized that various organisms may occur on an exposed coating, the specific types of organisms are mainly of academic interest. The degree to which microbiological discoloration occurs is the primary concern.
1.2 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.