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ASTM E2360-04

(Specification)

Specification for Cryptolaemus montrouzieri Mulsant (Coleoptera: Coccinellidae)

Specification for <i>Cryptolaemus montrouzieri</i> Mulsant (<i>Coleoptera: Coccinellidae</i>)

ABSTRACT
This specification covers information on and the test method for determining the purity, sex ratio, and number of adults in shipments of the predatory beetle Cryptolaemus montrouzieri (Order Coleoptera; Family Coccinellidae). Because the efficacy of the biological control of mealybug by C. montrouzieri depends on accurate release numbers, presence of male and female species, and absence of live product contaminants, accurate packaging and maintenance of purity and viability of C. montrouzieri shipments is, therefore, essential for the effective management of the target pests. This specification is intended for use by producers and end-users of the specified biological control agent.
SCOPE
1.1 This specification covers information on and the test method for determining purity, sex ratio, and number of adults in shipments of the predatory beetle Cryptolaemus montrouzieri.

General Information

Status
Historical
Publication Date
31-Mar-2004
ICS
07.080 - Biology. Botany. Zoology
Technical Committee
E35 - Pesticides, Antimicrobials, and Alternative Control Agents
Current Stage
Ref Project

Relations

Replaced By
ASTM E2360-04(2010) - Specification for <i>Cryptolaemus montrouzieri</i> Mulsant (<i>Coleoptera: Coccinellidae</i>) (Withdrawn 2019)
Effective Date
01-Oct-2010

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ASTM E2360-04 - Specification for <i>Cryptolaemus montrouzieri</i> Mulsant (<i>Coleoptera: Coccinellidae</i>)ASTM E2360-04 - Specification for <i>Cryptolaemus montrouzieri</i> Mulsant (<i>Coleoptera: Coccinellidae</i>)
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ASTM E2360-04 - Specification for <i>Cryptolaemus montrouzieri</i> Mulsant (<i>Coleoptera: Coccinellidae</i>)
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NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation: E2360 – 04
Standard Specification for
Cryptolaemus montrouzieri Mulsant (Coleoptera:
Coccinellidae)
This standard is issued under the fixed designation E2360; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 5.2 The number of C. montrouzieri will be determined by
examining a minimum of three units and determining the mean
1.1 This specification covers information on and the test
value. In each container, live contaminants will be identified
method for determining purity, sex ratio, and number of adults
and recorded.
in shipments of the predatory beetle Cryptolaemus montrouz-
ieri.
6. Significance and Use
2. Referenced Documents 6.1 The efficacy of mealybug control by C. montrouzieri
depends on accurate release numbers of beetles, presence of
2.1 ASTM Standards:
male and female beetles, and absence of live product contami-
E2200 Specification for Information Included with Packag-
nants. Accurate packaging and maintenance of purity and
ing of Multi-Cellular Biological Control Organisms
viability of C. montrouzieri shipments is, therefore, essential
3. Terminology
for the effective management of the target pest. This test
method is intended for use by producers and end-users of the
3.1 Definitions of Terms Specific to This Standard:
specified biological control agent. It is complementary to the
3.1.1 carrier—shredded paper.
quality guidelines for Cryptolaemus montrouzieri .
3.1.2 life stage when shipped—adult.
3.1.3 name of product—Cryptolaemus montrouzieri Mul-
7. Materials
sant.
7.1 Dissecting microscope or headband magnifier (7 to
3.1.4 preferred host prey—mealybugs.
103).
4. Classification 7.2 30 DR snap-top plastic vial.
7.3 Pharmaceutical pill counting tray.
4.1 Phylum—Arthropoda.
7.4 Fine-pointed No. 5/0 paint brush.
4.2 Class—Insecta.
4.3 Order—Coleoptera.
8. Test Unit
4.4 Family—Coccinellidae.
8.1 A single container with C. montrouzieri is considered a
4.5 Genus—Cryptolaemus.
test unit. A minimum of three containers per shipment will be
4.6 Species—montrouzieri.
randomly selected from the shipping box.
TEST METHOD—DETERMINING THE PURITY, SEX
9. Pre-Test Conditions
RATIO, AND NUMBER OFADULTS IN SHIPMENTS
OF C. montrouzieri 9.1 If samples must be held before testing, keep at 15°C,
relative humidity 60 to 90 %, for a maximum of 24 h.
5. Summary of Test Method
9.2 Chill beetles at 10°C for one hour prior to counting.
5.1 This test method describes a method of counting the
10. Sampling
number of C. montrouzieri beetles packaged in a carrier.
10.1 Specify the number of adults that are expected in each
test unit as indicated on the package before commencing test.
This specification is under the jurisdiction of ASTM Committee E35 on
Pesticides and Alternative Control Agents and is the direct responsibility of
11. Counting Procedure and Sex Ratio Determination
Subcommittee E35.30 on Natural Multi-Cellular (Metazoan) Biological Control
11.1 Carefullyremoveanypackingmaterialbyhand,allow-
Organisms.
Current edition approved April 1, 2004. Published April 2004. DOI: 10.1520/
ing the beetles to drop back into container. If beetles have been
E2360-04.
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
Standa
...

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