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ASTM D7391-17e1

(Test Method)

Standard Test Method for Categorization and Quantification of Airborne Fungal Structures in an Inertial Impaction Sample by Optical Microscopy

Standard Test Method for Categorization and Quantification of Airborne Fungal Structures in an Inertial Impaction Sample by Optical Microscopy

SIGNIFICANCE AND USE
5.1 This test method is used to estimate and categorize the number and type of fungal structures present on an inertial impactor sample.  
5.2 Fungal structures are identified and quantified regardless of whether they would or would not grow in culture.  
5.3 It must be emphasized that the detector in this test method is the analyst, and therefore results are subjective, depending on the experience, training, qualification, and mental and optical fatigue of the analyst.
SCOPE
1.1 This test method is a procedure that uses direct microscopy to analyze the deposit on an inertial impaction sample.  
1.2 This test method describes procedures for categorizing and enumerating fungal structures by morphological type. Typically, categories may be as small as genus (for example, Cladosporium) or as large as phylum (for example, basidiospores).  
1.3 This test method contains two procedures for enumerating fungal structures: one for slit impaction samples and one for circular impaction samples. This test method is applicable for impaction air samples, for which a known volume of air (at a rate as recommended by the manufacturer) has been drawn, and is also applicable for blank impaction samples.  
1.4 Enumeration results are presented in fungal structures/sample (fs/sample) and fungal structures/m3 (fs/m3).  
1.5 The range of enumeration results that can be determined with this test method depends on the size of the spores on the sample trace, the amount of particulate matter on the sample trace, the percentage of the sample trace counted, and the volume of air sampled.  
1.6 This test method addresses only the analysis of samples. The sampling process and interpretation of results is outside the scope of this test method.  
1.7 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.8 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.  
1.9 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

General Information

Status
Historical
Publication Date
14-Mar-2017
ICS
07.100.99 - Other standards related to microbiology
Technical Committee
D22 - Air Quality
Drafting Committee
D22.08 - Assessment, Sampling, and Analysis of Microorganisms
Current Stage
Ref Project

Relations

Replaces
ASTM D7391-17 - Standard Test Method for Categorization and Quantification of Airborne Fungal Structures in an Inertial Impaction Sample by Optical Microscopy
Effective Date
15-Mar-2017
Replaced By
ASTM D7391-20 - Standard Test Method for Categorization and Quantification of Airborne Fungal Structures in an Inertial Impaction Sample by Optical Microscopy
Effective Date
15-Mar-2020
Referred By
ASTM D8219-19 - Standard Guide for Cleaning and Disinfection at a Cannabis Cultivation Center
Effective Date
15-Mar-2017
Referred By
ASTM D1356-20a - Standard Terminology Relating to Sampling and Analysis of Atmospheres
Effective Date
15-Mar-2017
Referred By
ASTM D7788-14(2023) - Standard Practice for Collection of Total Airborne Fungal Structures via Inertial Impaction Methodology
Effective Date
15-Mar-2017

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ASTM D7391-17e1 - Standard Test Method for Categorization and Quantification of Airborne Fungal Structures in an Inertial Impaction Sample by Optical MicroscopyASTM D7391-17e1 - Standard Test Method for Categorization and Quantification of Airborne Fungal Structures in an Inertial Impaction Sample by Optical Microscopy
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ASTM D7391-17e1 - Standard Test Method for Categorization and Quantification of Airborne Fungal Structures in an Inertial Impaction Sample by Optical Microscopy
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REDLINE ASTM D7391-17e1 - Standard Test Method for Categorization and Quantification of Airborne Fungal Structures in an Inertial Impaction Sample by Optical MicroscopyREDLINE ASTM D7391-17e1 - Standard Test Method for Categorization and Quantification of Airborne Fungal Structures in an Inertial Impaction Sample by Optical Microscopy
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REDLINE ASTM D7391-17e1 - Standard Test Method for Categorization and Quantification of Airborne Fungal Structures in an Inertial Impaction Sample by Optical Microscopy
English language
13 pages
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Standards Content (Sample)

NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
´1
Designation:D7391 −17
Standard Test Method for
Categorization and Quantification of Airborne Fungal
Structures in an Inertial Impaction Sample by Optical
1
Microscopy
This standard is issued under the fixed designation D7391; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1
ε NOTE—Research report information added editorially in May 2017.
1. Scope priate safety and health practices and determine the applica-
bility of regulatory limitations prior to use.
1.1 This test method is a procedure that uses direct micros-
1.9 This international standard was developed in accor-
copy to analyze the deposit on an inertial impaction sample.
dance with internationally recognized principles on standard-
1.2 This test method describes procedures for categorizing
ization established in the Decision on Principles for the
and enumerating fungal structures by morphological type.
Development of International Standards, Guides and Recom-
Typically, categories may be as small as genus (for example,
mendations issued by the World Trade Organization Technical
Cladosporium) or as large as phylum (for example, basidi-
Barriers to Trade (TBT) Committee.
ospores).
2. Referenced Documents
1.3 This test method contains two procedures for enumer-
2
ating fungal structures: one for slit impaction samples and one 2.1 ASTM Standards:
for circular impaction samples. This test method is applicable D1193Specification for Reagent Water
forimpactionairsamples,forwhichaknownvolumeofair(at E691Practice for Conducting an Interlaboratory Study to
a rate as recommended by the manufacturer) has been drawn, Determine the Precision of a Test Method
and is also applicable for blank impaction samples.
3. Terminology
1.4 Enumeration results are presented in fungal structures/
3 3
3.1 ASTM Definitions (see the ASTM Online Dictionary of
sample (fs/sample) and fungal structures/m (fs/m ).
3
Engineering Science and Technology ):
1.5 Therangeofenumerationresultsthatcanbedetermined
3.1.1 numerical aperture.
with this test method depends on the size of the spores on the
3.2 Definitions of Terms Specific to This Standard:
sample trace, the amount of particulate matter on the sample
3.2.1 circular impaction sample, n—a sample of airborne
trace, the percentage of the sample trace counted, and the
particulatemattercollectedbymeansofadevicethatdrawsair
volume of air sampled.
through a round aperture at a specified rate, impacting the
1.6 Thistestmethodaddressesonlytheanalysisofsamples.
particles suspended in the air onto an adhesive medium,
The sampling process and interpretation of results is outside
resulting in a circular area of deposition. A circular impaction
the scope of this test method.
sample may be collected by means of a cassette manufactured
1.7 The values stated in SI units are to be regarded as forthatpurpose,orbymeansofasamplingdevicethatrequires
standard. No other units of measurement are included in this slides to be pre-coated with impaction medium.
standard.
3.2.2 debris rating, n—a distinct value assigned to an
1.8 This standard does not purport to address all of the impactor sample based on the percentage of the sample area
safety concerns, if any, associated with its use. It is the potentially obscured by particulate matter, and ranging from 0
responsibility of the user of this standard to establish appro- to 5.
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
1
This test method is under the jurisdiction of ASTM Committee D22 on Air contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
Quality and is the direct responsibility of Subcommittee D22.08 on Sampling and Standards volume information, refer to the standard’s Document Summary page on
Analysis of Mold. the ASTM website.
3
Current edition approved March 15, 2017. Published April 2017. Originally ASTM Online Dictionary of Engineering Science and Technology (Stock #:
approved in 2009. Last previous edition approved in 2009 as D7391 – 09. DOI: DEFONLINE) is available on theASTM website, www.astm.org, or contactASTM
10.1520/D7391-17E01. Customer Service at service@astm.org.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

---------------------- Page: 1 ----------------------
´1
D7391−17
3.2.3 field blank, n—asampleslideorcassettecarriedtothe forthatpurpose,orbymeansofasamplingdevicethatrequires
sampling site, exposed to sampling conditions (for example, slides to be pre-coated with impaction medium.
seals opened), returned to the laboratory, treated as a sample,
3.2.19 spor
...

This document is not an ASTM standard and is intended only to provide the user of an ASTM standard an indication of what changes have been made to the previous version. Because
it may not be technically possible to adequately depict all changes accurately, ASTM recommends that users consult prior editions as appropriate. In all cases only the current version
of the standard as published by ASTM is to be considered the official document.
´1
Designation: D7391 − 17 D7391 − 17
Standard Test Method for
Categorization and Quantification of Airborne Fungal
Structures in an Inertial Impaction Sample by Optical
1
Microscopy
This standard is issued under the fixed designation D7391; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1
ε NOTE—Research report information added editorially in May 2017.
1. Scope
1.1 This test method is a procedure that uses direct microscopy to analyze the deposit on an inertial impaction sample.
1.2 This test method describes procedures for categorizing and enumerating fungal structures by morphological type. Typically,
categories may be as small as genus (for example, Cladosporium) or as large as phylum (for example, basidiospores).
1.3 This test method contains two procedures for enumerating fungal structures: one for slit impaction samples and one for
circular impaction samples. This test method is applicable for impaction air samples, for which a known volume of air (at a rate
as recommended by the manufacturer) has been drawn, and is also applicable for blank impaction samples.
3 3
1.4 Enumeration results are presented in fungal structures/sample (fs/sample) and fungal structures/m (fs/m ).
1.5 The range of enumeration results that can be determined with this test method depends on the size of the spores on the
sample trace, the amount of particulate matter on the sample trace, the percentage of the sample trace counted, and the volume
of air sampled.
1.6 This test method addresses only the analysis of samples. The sampling process and interpretation of results is outside the
scope of this test method.
1.7 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.8 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility
of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory
limitations prior to use.
1.9 This international standard was developed in accordance with internationally recognized principles on standardization
established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued
by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
2. Referenced Documents
2
2.1 ASTM Standards:
D1193 Specification for Reagent Water
E691 Practice for Conducting an Interlaboratory Study to Determine the Precision of a Test Method
3. Terminology
3
3.1 ASTM Definitions (see the ASTM Online Dictionary of Engineering Science and Technology ):
3.1.1 numerical aperture.
3.2 Definitions of Terms Specific to This Standard:
1
This test method is under the jurisdiction of ASTM Committee D22 on Air Quality and is the direct responsibility of Subcommittee D22.08 on Sampling and Analysis
of Mold.
Current edition approved March 15, 2017. Published April 2017. Originally approved in 2009. Last previous edition approved in 2009 as D7391 – 09. DOI:
10.1520/D7391-17.10.1520/D7391-17E01.
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM Standards
volume information, refer to the standard’s Document Summary page on the ASTM website.
3
ASTM Online Dictionary of Engineering Science and Technology (Stock #: DEFONLINE) is available on the ASTM website, www.astm.org, or contact ASTM Customer
Service at service@astm.org.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

---------------------- Page: 1 ----------------------
´1
D7391 − 17
3.2.1 circular impaction sample, n—a sample of airborne particulate matter collected by means of a device that draws air
through a round aperture at a specified rate, impacting the particles suspended in the air onto an adhesive medium, resulting in a
circular area of deposition. A circular impaction sample may be collected by means of a cassette manufactured for that purpose,
or by means of a sampling device that requires slides to be pre-coated with impaction medium.
3.2.2 debris rating, n—a distinct value assigned to an impactor sample based on the percentage of the sample area potentially
...

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Standard Guide for Developing Methodology for Evaluating the Ability of Indoor Materials to Support Microbial Growth Using Static Environmental Chambers

SIGNIFICANCE AND USE
4.1 The static chambers have several different applications:  
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SCOPE
1.1 Many different types of microorganisms (for example, bacteria, fungi, viruses, algae) can occupy indoor spaces. Materials that support microbial growth are potential indoor sources of biocontaminants (for example, spores and toxins) that can become airborne indoor biopollutants. This guide describes a simple, relatively cost effective approach to evaluating the ability of a variety of materials to support microbial growth using a small chamber method.  
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SCOPE
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SCOPE
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SCOPE
1.1 This test method covers the enumeration of bacteria that can hydrolyze protein/collagen in fresh (uncured) hides and skins. This test method is applicable to uncured hides and skins.  
1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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  • Standard
    7 pages
    English language
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ASTM
ASTM D7816-23(Test Method)
Standard Test Method for Enumeration of Halophilic and Proteolytic Bacteria in Raceway Brine, Brine-Cured Hides and Skins

SIGNIFICANCE AND USE
4.1 This test method enumerates salt tolerant (halophilic) bacteria, and proteolytic bacteria that are also salt tolerant. Under the conditions of this test method those bacteria are equated as halophilic organisms. Salt tolerant proteolytic bacteria have been known to cause damage to hides and skins in raceway brine.
SCOPE
1.1 This test method covers the enumeration of bacteria that can tolerate high salt concentrations or can hydrolyze protein/collagen, or both. This test method is applicable to raceway brine, brine-cured hides and skins, and pre-charge raceway liquor.  
1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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  • Standard
    7 pages
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ASTM
ASTM E2471-23(Test Method)
Standard Test Method for Using Seeded-Agar for the Screening Assessment of Antimicrobial Activity In Carpets

SIGNIFICANCE AND USE
5.1 This test method provides for rapid screening of antimicrobial treatments located in or on the carpet face fiber or incorporated into the backing structure of the carpet (or both).  
5.2 This test method simulates actual use conditions that may occur on carpets (for example, food and beverage spills, soiling from foot traffic, prolonged moisture exposure).  
5.3 This test method provides a means to screen for activity and durability of an antimicrobial treatment under conditions of organic loading.  
5.4 This test method provides for the simultaneous assessment of multiple carpet components for antimicrobial activity.  
5.5 Carpets may be cleaned prior to testing with this test method in order to assess the durability of the antimicrobial effect.
SCOPE
1.1 This test method is designed to evaluate (qualitatively) the presence of antimicrobial activity in or on carpets. Use this test method to qualitatively evaluate both antibacterial and antifungal activity.  
1.2 Use half strength (nutrient and agar) tryptic soy agar as the inoculum vehicle for bacteria and half strength potato dextrose agar as the inoculum vehicle for mold conidia. Use of half strength agars may reduce undue neutralization of an antimicrobial due to excessive organic load.  
1.3 This test method simultaneously evaluates (both visual and stereo-microscopic) antimicrobial activity both at the fiber layer and at the primary backing layer of carpet.  
1.4 Use this test method to assess the durability of the antimicrobial treatments on new carpets, and on those repeatedly shampooed or exposed to in-use conditions.  
1.5 Knowledge of microbiological techniques is required for the practice of this test method.  
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

  • Standard
    5 pages
    English language
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  • Standard
    5 pages
    English language
    sale 15% off