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ASTM E1045-00(2005)

(Specification)

Standard Specification for Pipet, Sahli Hemoglobin

Standard Specification for Pipet, Sahli Hemoglobin

ABSTRACT
This specification covers reusable Sahli hemoglobin pipets. The pipets shall be made of common spirit bore white back tubing. Pipet design shall be straight and of one-piece construction. Pipet delivery tips shall be made with a gradual or concave taper. Pipet dimensions shall be within tolerance limit indicated in this specification. The pipet shall have markings which include graduation line, volumetric designation, identification, and capacity deviation. Testing methods include capacity test, capacity deviation, pigmentation test, and strain free test.
SCOPE
1.1 This specification covers reusable pipets calibrated" to contain" 20 cmm of whole blood and used for hemoglobin determinations.

General Information

Status
Historical
Publication Date
09-Nov-2000
Technical Committee
E41 - Laboratory Apparatus
Drafting Committee
E41.01 - Laboratory Ware and Supplies
Current Stage
Ref Project

Relations

Replaces
ASTM E1045-00 - Standard Specification for Pipet, Sahli Hemoglobin
Effective Date
10-Nov-2000
Replaced By
ASTM E1045-00(2010) - Standard Specification for Pipet, Sahli Hemoglobin
Effective Date
01-Jul-2010
Referred By
ASTM E694-18 - Standard Specification for Laboratory Glass Volumetric Apparatus
Effective Date
10-Nov-2000

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ASTM E1045-00(2005) - Standard Specification for Pipet, Sahli HemoglobinASTM E1045-00(2005) - Standard Specification for Pipet, Sahli Hemoglobin
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ASTM E1045-00(2005) - Standard Specification for Pipet, Sahli Hemoglobin
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Standards Content (Sample)

NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation: E1045 – 00 (Reapproved 2005)
Standard Specification for
1
Pipet, Sahli Hemoglobin
This standard is issued under the fixed designation E1045; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 5. Markings
1.1 This specification covers reusable pipets calibrated“ to 5.1 Graduation Line—The pipet shall have one graduation
contain” 20 cmm of whole blood and used for hemoglobin line located 20 cmm from the pipet tip. The graduation line
determinations. shall be located on the clear portion of the tubing and shall
extend at least two thirds around the pipet and not exceed 0.4
2. Referenced Documents
mm in width.
2
2.1 ASTM Standards:
5.2 Volumetric Designation—The pipet shall be marked
E920 Specification for Commercially Packaged Laboratory 20CMM on the clear portion of the tubing with the markings
Apparatus
located approximately 5 mm above the graduation line.
E921 Specification for Export Packaged Laboratory Appa- 5.3 Identification—Each pipet shall be marked with the
ratus
manufacturer’s name or trademark on the white stripe portion
E1133 Practice for Performance Testing of Packaged Labo- of the pipet. Catalog number markings are optional. All
ratory Apparatus for United States Government Procure-
markings shall be permanently fused onto the pipet. The
ments markings shall be amber or black in color. When tested in
E1157 Specification for Sampling and Testing of Reusable
accordance to 6.3, the pigmentation shall not discolor. The
Laboratory Glassware appearance of the markings, when viewed by the eye under
normal room lighting, shall be the same before and after
3. Materials
testing.
3.1 The pipets shall be made of common spirit bore white
5.4 Capacity Deviation—SahliHemoglobinpipetsaremade
back tubing or of clear glass with a white stripe applied to the
with maximum capacity deviation of 6 1.0% or6 2.0%. The
outer surface of the tubing.
selected capacity deviation shall be marked on the clear or
whitestripeportionofthepipet.Thecapacityofthepipetshall
4. Design
be within the selected capacity deviation marked on the pipet
4.1 Shape—Pipets shall be straight and of one-piece con-
when tested as specified in 6.2.
struction. Any cross section of a pipet taken in a plane
6. Testing
perpendicular to the longitudinal axis should be circular.
4.2 Delivery Tips—Delivery tips shall be made with a
6.1 Capacity Test—The capacity of the pipet shall be
gradual or concave taper to a length of 10 to 25 mm. The tip
determined by means of using distilled water and a weighing
end shall be ground and tapered with fine abrasive, or fire-
device with weight sensitivity not less than 0.001 mg.
polished.Dimensionsofthedeliverytipshallbeasspecifiedin
6.1.1 The pipet shall be thoroughly cleaned, dried, and
Fig. 1.
allowed to adjust to room temperature.
4.3 Thetopofthepipetshallbegroundtoataperorformed
6.1.2 The pipet shall be weighed and the weight recorded.
to a funnel shape according to the dimensions specified in Fig.
6.1.3 The pipet shall be filled to the calibration line with
1.
distilled water and weighed, and the weight recorded.
6.1.4 Therecordedweightofthecleananddrypipetshallbe
subtractedfromtherecordedweightofthedistilledwater-filled
1
This specification is under the jurisdiction of ASTM Committee E41 on
pipet providing the observed volumetric capacity (V)ofthe
Labor
...

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