ASTM E1292-94(2016)
(Specification)Standard Specification for Gravity Convection and Forced Ventilation Incubators
Standard Specification for Gravity Convection and Forced Ventilation Incubators
ABSTRACT
This specification covers the performance requirements for general-purpose air incubators ordinarily used for incubating procedures. It is applicable to gravity and forced ventilation incubators designed to operate over a specified range of temperature. This specification does not include any requirements for the safe handling of harmful or disease bearing organisms. Temperature uniformity within a specified period of time shall be tested using several thermocouples.
SCOPE
1.1 This specification covers the performance requirements for general purpose air incubators ordinarily used for incubating procedures, which have an incubating chamber up to 0.6 m3 (25 ft3) in volume. It is applicable to gravity and forced ventilation incubators designed to operate over all or part of the temperature range from 5°C above ambient to 75°C.
1.2 This specification does not include any requirements for the safe handling of harmful or disease bearing organisms.
1.3 The following precautionary caveat pertains only to the test method portions, Sections 4, 5, and 6, of this specification. This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
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Designation:E1292 −94 (Reapproved 2016)
Standard Specification for
1
Gravity Convection and Forced Ventilation Incubators
This standard is issued under the fixed designation E1292; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 3.2 The rate of ventilation of the incubating chamber shall
conform to the requirements specified in Table 1 for the
1.1 This specification covers the performance requirements
particular type of incubator when measured in accordance with
for general purpose air incubators ordinarily used for incubat-
the procedure given in Section 5.
ing procedures, which have an incubating chamber up to 0.6
3 3
m (25 ft ) in volume. It is applicable to gravity and forced
3.3 The recovery time of the incubating chamber shall
ventilationincubatorsdesignedtooperateoverallorpartofthe conform to the requirements specified in Table 1 for the
temperature range from 5°C above ambient to 75°C.
particulartypeofincubator,whentestedinaccordancewiththe
procedure specified in Section 6.
1.2 This specification does not include any requirements for
the safe handling of harmful or disease bearing organisms.
4. Test Methods
1.3 The following precautionary caveat pertains only to the
4.1 Temperature Uniformity:
test method portions, Sections 4, 5, and 6, of this specification.
4.1.1 Place nine calibrated thermocouples (Note 1) made
This standard does not purport to address all of the safety
from iron-constantan or copper-constantan wires approxi-
concerns, if any, associated with its use. It is the responsibility
mately 0.5 mm in diameter (No. 24 gauge) and having a
of the user of this standard to establish appropriate safety and
junction size of not more than 2 mm (0.08 in.) in the empty
health practices and determine the applicability of regulatory
incubating chamber with shelves in place, and vents open.
limitations prior to use.
Locate one thermocouple in each of the eight corners of the
incubator approximately 5 cm (2 in.) from each wall, and place
2. Types
the ninth thermocouple within 2.5 cm (1 in.) of the geometric
2.1 This specification covers the following four types of air
center of the chamber. A minimum length of 30 cm (12 in.) of
incubators:
wire for each thermocouple shall be inside the incubator to
2.1.1 Type 1A—An incubator ventilated by gravity convec-
minimize the conduction of heat from the thermocouple.
tion having a uniformity of temperature within 3 % of the
differential between incubator and ambient temperature, NOTE 1—If calibrated thermocouples are not available, nine thermo-
couples made from the same spool of wire may be used, provided they
2.1.2 Type 1B—An incubator ventilated by gravity convec-
give the same value for temperature when placed adjacent to one another
tion having a uniformity of temperature within 6 % of the
in the incubating chamber at the temperature of the test.
differential between incubator and ambient temperature,
4.1.2 Bring the incubator to the specified temperature and
2.1.3 Type 2A—An incubator ventilated by forced ventila-
allow it to reach a steady state (Note 2). Record the tempera-
tion having a uniformity of temperature within 2 % of the
ture of the nine thermocouples for a period of at least 12 h and
differential between incubator and ambient temperature,
determine from the record the maximum deviation of each
2.1.4 Type 2B—An incubator ventilated by forced ventila-
point from the desired temperature. The ambient room tem-
tion having a uniformity of temperature within 5 % of the
perature shall vary by not more than a total of 5°C and the line
differential between incubator and ambient temperature.
voltage shall not vary by more than a total of 5 % during the
3. Performance Requirements
test.
3.1 The temperature within the incubating chamber shall be
NOTE 2—Some incubators may require as much as 12 h to reach a
controlled by an automatic device, and shall be uniform within
steady state. If a steady state does not exist, there is a drift in the
the tolerances given in Table 1, when tested in accordance with
temperature toward the steady state condition.
Section 4.
5. Rate of Ventilation
1
5.1 Seal the ventilation ports, doors, and all apertures of the
This specification is under the jurisdiction of ASTM Committee E41 on
Laboratory Apparatus and is the direct responsibility of Subcommittee E41.06 on
incubator with adhesive tape, or by other means to prevent any
Laboratory Instruments and Equipment.
air from passing through the incubator (Note 3). Connect a Wh
Current edition approved July 1, 2016. Published August 2016. Originally
meter with the smalle
...
This document is not an ASTM standard and is intended only to provide the user of an ASTM standard an indication of what changes have been made to the previous version. Because
it may not be technically possible to adequately depict all changes accurately, ASTM recommends that users consult prior editions as appropriate. In all cases only the current version
of the standard as published by ASTM is to be considered the official document.
Designation: E1292 − 94 (Reapproved 2011) E1292 − 94 (Reapproved 2016)
Standard Specification for
1
Gravity Convection and Forced Ventilation Incubators
This standard is issued under the fixed designation E1292; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope
1.1 This specification covers the performance requirements for general purpose air incubators ordinarily used for incubating
3 3
procedures, which have an incubating chamber up to 0.6 m (25 ft ) in volume. It is applicable to gravity and forced ventilation
incubators designed to operate over all or part of the temperature range from 5°C above ambient to 75°C.
1.2 This specification does not include any requirements for the safe handling of harmful or disease bearing organisms.
1.3 The following precautionary caveat pertains only to the test method portions, Sections 4, 5, and 6, of this specification.This
standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user
of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior
to use.
2. Types
2.1 This specification covers the following four types of air incubators:
2.1.1 Type 1A—An incubator ventilated by gravity convection having a uniformity of temperature within 3 % of the differential
between incubator and ambient temperature,
2.1.2 Type 1B—An incubator ventilated by gravity convection having a uniformity of temperature within 6 % of the differential
between incubator and ambient temperature,
2.1.3 Type 2A—An incubator ventilated by forced ventilation having a uniformity of temperature within 2 % of the differential
between incubator and ambient temperature,
2.1.4 Type 2B—An incubator ventilated by forced ventilation having a uniformity of temperature within 5 % of the differential
between incubator and ambient temperature.
3. Performance Requirements
3.1 The temperature within the incubating chamber shall be controlled by an automatic device, and shall be uniform within the
tolerances given in Table 1, when tested in accordance with Section 4.
3.2 The rate of ventilation of the incubating chamber shall conform to the requirements specified in Table 1 for the particular
type of incubator when measured in accordance with the procedure given in Section 5.
3.3 The recovery time of the incubating chamber shall conform to the requirements specified in Table 1 for the particular type
of incubator, when tested in accordance with the procedure specified in Section 6.
4. Test Methods
4.1 Temperature Uniformity:
4.1.1 Place nine calibrated thermocouples (Note 1) made from iron-constantan or copper-constantan wires approximately 0.5
mm in diameter (No. 24 gauge) and having a junction size of not more than 2 mm (0.08 in.) in the empty incubating chamber with
shelves in place, and vents open. Locate one thermocouple in each of the eight corners of the incubator approximately 5 cm (2
in.) from each wall, and place the ninth thermocouple within 2.5 cm (1 in.) of the geometric center of the chamber. A minimum
length of 30 cm (12 in.) of wire for each thermocouple shall be inside the incubator to minimize the conduction of heat from the
thermocouple.
NOTE 1—If calibrated thermocouples are not available, nine thermocouples made from the same spool of wire may be used, provided they give the
1
This specification is under the jurisdiction of ASTM Committee E41 on Laboratory Apparatus and is the direct responsibility of Subcommittee E41.06 on Weighing
DevicesLaboratory Instruments and Equipment.
Current edition approved Dec. 1, 2011July 1, 2016. Published December 2011August 2016. Originally approved in 1989. Last previous edition approved in 20062011 as
E1292 – 94 (2006).(2011). DOI: 10.1520/E1292-94R11.10.1520/E1292-94R16.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
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E1292 − 94 (2016)
TABLE 1 Performance Requirements for Incubators
Characteristic Type 1A Type 1B Type 2A Type 2B
Deviation from specified temperature of test throughout incubating chamber 3 (0.4°C) 6 (0.7°C) 2 (0.25°C) 5 (0.6°C)
during the 12 h period. Maximum percent of the differential between ambie
...
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