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ASTM E3029-15

(Practice)

Standard Practice for Determining Relative Spectral Correction Factors for Emission Signal of Fluorescence Spectrometers

Standard Practice for Determining Relative Spectral Correction Factors for Emission Signal of Fluorescence Spectrometers

SIGNIFICANCE AND USE
3.1 Calibration of the responsivity of the detection system for emission (EM) as a function of EM wavelength (λEM), also referred to as spectral correction of emission, is necessary for successful quantification when intensity ratios at different EM wavelengths are being compared or when the true shape or peak maximum position of an EM spectrum needs to be known. Such calibration methods are given here and summarized in Table 1. This type of calibration is necessary because the spectral responsivity of a detection system can change significantly over its useful wavelength range (see Fig. 1). It is highly recommended that the wavelength accuracy (see Test Method E388) and the linear range of the detection system (see Guide E2719 and Test Method E578) be determined before spectral calibration is performed and that appropriate steps are taken to insure that all measured intensities during this calibration are within the linear range. For example, when using wide slit widths in the monochromators, attenuators may be needed to attenuate the excitation beam or emission, thereby, decreasing the fluorescence intensity at the detector. Also note that when using an EM polarizer, the spectral correction for emission is dependent on the polarizer setting. (2) It is important to use the same instrument settings for all of the calibration procedures mentioned here, as well as for subsequent sample measurements.  
3.2 When using CCD or diode array detectors with a spectrometer for λEM selection, the spectral correction factors are dependent on the grating position of the spectrometer. Therefore, the spectral correction profile versus λEM must be determined separately for each grating position used. (3)  
3.3 Instrument manufacturers often provide an automated procedure and calculation for a spectral correction function for emission, or they may supply a correction that was determined at the factory. This correction can often be applied during spectral collection or as a post-co...
SCOPE
1.1 This practice (1)2 describes three methods for determining the relative spectral correction factors for grating-based fluorescence spectrometers in the ultraviolet-visible spectral range. These methods are intended for instruments with a 0°/90° transmitting sample geometry. Each method uses different types of transfer standards, including 1) a calibrated light source (CS), 2) a calibrated detector (CD) and a calibrated diffuse reflector (CR), and 3) certified reference materials (CRMs). The wavelength region covered by the different methods ranges from 250 to 830 nm with some methods having a broader range than others. Extending these methods to the near infrared (NIR) beyond 830 nm will be discussed briefly, where appropriate. These methods were designed for scanning fluorescence spectrometers with a single channel detector, but can also be used with a multichannel detector, such as a diode array or a CCD.  
1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

General Information

Status
Historical
Publication Date
31-Aug-2015
ICS
17.180.01 - Optics and optical measurements in general
Technical Committee
E13 - Molecular Spectroscopy and Separation Science
Drafting Committee
E13.01 - Ultra-Violet, Visible, and Luminescence Spectroscopy
Current Stage
Ref Project

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ASTM E3029-15 - Standard Practice for Determining Relative Spectral Correction Factors for Emission Signal of Fluorescence SpectrometersASTM E3029-15 - Standard Practice for Determining Relative Spectral Correction Factors for Emission Signal of Fluorescence Spectrometers
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ASTM E3029-15 - Standard Practice for Determining Relative Spectral Correction Factors for Emission Signal of Fluorescence Spectrometers
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NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation:E3029 −15
Standard Practice for
Determining Relative Spectral Correction Factors for
1
Emission Signal of Fluorescence Spectrometers
This standard is issued under the fixed designation E3029; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope E578 Test Method for Linearity of Fluorescence Measuring
2
Systems
1.1 This practice (1) describes three methods for determin-
E2719 Guide for Fluorescence—Instrument Calibration and
ing the relative spectral correction factors for grating-based
Qualification
fluorescence spectrometers in the ultraviolet-visible spectral
range. These methods are intended for instruments with a
3. Significance and Use (Intro)
0°/90° transmitting sample geometry. Each method uses dif-
ferenttypesoftransferstandards,including 1)acalibratedlight
3.1 Calibration of the responsivity of the detection system
source (CS), 2) a calibrated detector (CD) and a calibrated
for emission (EM) as a function of EM wavelength (λ ), also
EM
diffuse reflector (CR), and 3) certified reference materials
referred to as spectral correction of emission, is necessary for
(CRMs). The wavelength region covered by the different
successful quantification when intensity ratios at different EM
methodsrangesfrom250to830nmwithsomemethodshaving
wavelengths are being compared or when the true shape or
a broader range than others. Extending these methods to the
peak maximum position of an EM spectrum needs to be
near infrared (NIR) beyond 830 nm will be discussed briefly,
known. Such calibration methods are given here and summa-
where appropriate. These methods were designed for scanning
Table 1. This type of calibration is necessary because
rized in
fluorescence spectrometers with a single channel detector, but
the spectral responsivity of a detection system can change
can also be used with a multichannel detector, such as a diode
significantly over its useful wavelength range (see Fig. 1). It is
array or a CCD.
highly recommended that the wavelength accuracy (see Test
1.2 The values stated in SI units are to be regarded as
MethodE388)andthelinearrangeofthedetectionsystem(see
standard. No other units of measurement are included in this
Guide E2719 and Test Method E578) be determined before
standard.
spectral calibration is performed and that appropriate steps are
1.3 This standard does not purport to address all of the
taken to insure that all measured intensities during this cali-
safety concerns, if any, associated with its use. It is the
bration are within the linear range. For example, when using
responsibility of the user of this standard to establish appro-
wide slit widths in the monochromators, attenuators may be
priate safety and health practices and determine the applica-
needed to attenuate the excitation beam or emission, thereby,
bility of regulatory limitations prior to use.
decreasing the fluorescence intensity at the detector.Also note
that when using an EM polarizer, the spectral correction for
2. Referenced Documents
emission is dependent on the polarizer setting. (2)Itis
3
2.1 ASTM Standards:
important to use the same instrument settings for all of the
E131 Terminology Relating to Molecular Spectroscopy
calibration procedures mentioned here, as well as for subse-
E388 Test Method for Wavelength Accuracy and Spectral
quent sample measurements.
Bandwidth of Fluorescence Spectrometers
3.2 When using CCD or diode array detectors with a
spectrometer for λ selection, the spectral correction factors
EM
1
This practice is under the jurisdiction of ASTM Committee E13 on Molecular
are dependent on the grating position of the spectrometer.
Spectroscopy and Separation Science and is the direct responsibility of Subcom-
Therefore, the spectral correction profile versus λ must be
EM
mittee E13.01 on Ultra-Violet, Visible, and Luminescence Spectroscopy.
determined separately for each grating position used. (3)
Current edition approved Sept. 1, 2015. Published October 2015. DOI: 10.1520/
E3029-15
2 3.3 Instrument manufacturers often provide an automated
The boldface numbers in parentheses refer to a list of references at the end of
this standard. procedure and calculation for a spectral correction function for
3
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
emission, or they may supply a correction that was determined
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
at the factory. This correction can often be applied during
Standards volume information, refer to the standard’s Document Summary page on
the ASTM website. spectral collection or as a post
...

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Standard Practice for Determining Relative Spectral Correction Factors for Emission Signal of Fluorescence Spectrometers

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3.1 Calibration of the responsivity of the detection system for emission (EM) as a function of EM wavelength (λEM), also referred to as spectral correction of emission, is necessary for successful quantification when intensity ratios at different EM wavelengths are being compared or when the true shape or peak maximum position of an EM spectrum needs to be known. Such calibration methods are given here and summarized in Table 1. This type of calibration is necessary because the spectral responsivity of a detection system can change significantly over its useful wavelength range (see Fig. 1). It is highly recommended that the wavelength accuracy (see Test Method E388) and the linear range of the detection system (see Guide E2719 and Test Method E578) be determined before spectral calibration is performed and that appropriate steps are taken to insure that all measured intensities during this calibration are within the linear range. For example, when using wide slit widths in the monochromators, attenuators may be needed to attenuate the excitation beam or emission, thereby, decreasing the fluorescence intensity at the detector. Also note that when using an EM polarizer, the spectral correction for emission is dependent on the polarizer setting. (2) It is important to use the same instrument settings for all of the calibration procedures mentioned here, as well as for subsequent sample measurements.  
FIG. 1 Example of Relative Spectral Responsivity of Emission Detection System (Grating Monochromator-PMT Based), (see Test Method E578) for which a Correction Needs to be Applied to a Measured Instrument-Specific Emission Spectrum to Obtain its True Spectral Shape (Relative Intensities).  
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1.1 This practice (1)2 describes three methods for determining the relative spectral correction factors for grating-based fluorescence spectrometers in the ultraviolet-visible spectral range. These methods are intended for instruments with a 0°/90° transmitting sample geometry. Each method uses different types of transfer standards, including 1) a calibrated light source (CS), 2) a calibrated detector (CD) and a calibrated diffuse reflector (CR), and 3) certified reference materials (CRMs). The wavelength region covered by the different methods ranges from 250 nm to 830 nm with some methods having a broader range than others. Extending these methods to the near infrared (NIR) beyond 830 nm will be discussed briefly, where appropriate. These methods were designed for scanning fluorescence spectrometers with a single channel detector, but can also be used with a multichannel detector, such as a diode array or a CCD.  
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3.1 Calibration of the responsivity of the detection system for emission (EM) as a function of EM wavelength (λEM), also referred to as spectral correction of emission, is necessary for successful quantification when intensity ratios at different EM wavelengths are being compared or when the true shape or peak maximum position of an EM spectrum needs to be known. Such calibration methods are given here and summarized in Table 1. This type of calibration is necessary because the spectral responsivity of a detection system can change significantly over its useful wavelength range (see Fig. 1). It is highly recommended that the wavelength accuracy (see Test Method E388) and the linear range of the detection system (see Guide E2719 and Test Method E578) be determined before spectral calibration is performed and that appropriate steps are taken to insure that all measured intensities during this calibration are within the linear range. For example, when using wide slit widths in the monochromators, attenuators may be needed to attenuate the excitation beam or emission, thereby, decreasing the fluorescence intensity at the detector. Also note that when using an EM polarizer, the spectral correction for emission is dependent on the polarizer setting. (2) It is important to use the same instrument settings for all of the calibration procedures mentioned here, as well as for subsequent sample measurements.  
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5.1 With the advent of thick, highly angled aircraft transparencies, multiple imaging has been more frequently cited as an optical problem by pilots. Secondary images (of outside lights), often varying in intensity and displacement across the windscreen, can give the pilot deceptive optical cues of his altitude, velocity, and approach angle, increasing his visual workload. Current specifications for multiple imaging in transparencies are vague and not quantitative. Typical specifications state “multiple imaging shall not be objectionable.”  
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SCOPE
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SCOPE
1.1 This guide covers the use of oil spill dispersants to assist in the control of oil spills. The guide is written with the goal of minimizing the environmental impacts of oil spills; this goal is the basis on which the recommendations are made. Aesthetic and socioeconomic factors are not considered, although these and other factors are often important in spill response.  
1.2 Spill responders have available several means to control or clean up spilled oil. Chemical dispersants should be given equal consideration with other spill countermeasures.  
1.3 This is a general guide only. Oil, as used in this guide, includes crude oils and refined petroleum products. Differences between individual dispersants or between different oil products are not considered. The dispersibility of the oil with the chosen dispersant should be evaluated.  
1.4 The guide is organized by habitat type, for example, small ponds and lakes, rivers and streams, and land. It considers the use of dispersants primarily to protect habitats from impact (or to minimize impacts).  
1.5 This guide applies only to freshwater and other inland environments. It does not consider the direct application of dispersants to subsurface waters.  
1.6 In making dispersant use decisions, appropriate government authorities should be consulted as required by law.  
1.7 This guide does not address getting regulatory approval.  
1.8 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.9 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.10 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

  • Guide
    3 pages
    English language
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  • Guide
    3 pages
    English language
    sale 15% off