Standard Practice for Computing the Colors of Fluorescent Objects from Bispectral Photometric Data

SIGNIFICANCE AND USE
The bispectral or two-monochromator method is the definitive method for the determination of the general radiation-transfer properties of fluorescent specimens (4). In this method, the measuring instrument is equipped with two separate monochromators. The first, the irradiation monochromator, irradiates the specimen with monochromatic light. The second, the viewing monochromator, analyzes the radiation leaving the specimen. A two-dimensional array of bispectral photometric values is obtained by setting the irradiation monochromator at a series of fixed wavelengths (μ) in the ultraviolet and visible range, and for each μ, using the viewing monochromator to record readings for each wavelength (λ) in the visible range. The resulting array, once properly corrected, is known as the Donaldson matrix, and the value of each element (μ,λ) of this array is here described as the Donaldson radiance factor (D(μ,λ)). The Donaldson radiance factor is an instrument- and illuminant-independent photometric property of the specimen, and can be used to calculate its color for any desired illuminant and observer. The advantage of this method is that it provides a comprehensive characterization of the specimen's radiation-transfer properties, without the inaccuracies associated with source simulation and various methods of approximation.
SCOPE
1.1 This practice provides the values and practical computation procedures needed to obtain tristimulus values, designated X, Y, Z and X10, Y10, Z10 for the CIE 1931 and 1964 observers, respectively, from bispectral photometric data for the specimen. Procedures for obtaining such bispectral photometric data are contained in Practice E2153.
1.2 Procedures for conversion of results to color spaces that are part of the CIE system, such as CIELAB and CIELUV are contained in Practice E308.
1.3 This standard may involve hazardous materials, operations, and equipment. This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

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Publication Date
30-Jun-2012
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Drafting Committee
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NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation: E2152 − 12
Standard Practice for
Computing the Colors of Fluorescent Objects from
1
Bispectral Photometric Data
This standard is issued under the fixed designation E2152; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
INTRODUCTION
The fundamental procedure for evaluating the color of a fluorescent specimen is to obtain bispectral
photometric data for specified irradiating and viewing geometries, and from these data to compute
tristimulus values based on a CIE (International Commission on Illumination) standard observer and
a CIE standard illuminant. Procedures for such computation are contained in this practice. This
practice also contains procedures for computing illuminant-specific spectral radiance factor values
from illuminant-independent bispectral photometric data.
1. Scope for Evaluation of Fluorescent Color
2.2 CIE Standards:
1.1 This practice provides the values and practical compu-
3
tation procedures needed to obtain tristimulus values, desig- CIE Publication 15.2, Colorimetry
nated X, Y, Z and X ,Y ,Z for the CIE 1931 and 1964
10 10 10 2.3 ISO Standards:
observers, respectively, from bispectral photometric data for
ISO 11476 Paper and Board—Determination of CIE-
4
the specimen. Procedures for obtaining such bispectral photo-
Whiteness, C/2 Degrees
metric data are contained in Practice E2153.
3. Terminology
1.2 Procedures for conversion of results to color spaces that
are part of the CIE system, such as CIELAB and CIELUV are
3.1 Definitions—The definitions contained in Terminology
contained in Practice E308.
E284 are applicable to this practice.
1.3 This standard may involve hazardous materials,
3.2 Definitions of Terms Specific to This Standard:
operations, and equipment. This standard does not purport to
3.2.1 bispectrometer, n—an optical instrument equipped
address all of the safety concerns, if any, associated with its
with a source of irradiation, two monochromators, and a
use. It is the responsibility of the user of this standard to
detection system, such that a specimen can be measured at
establish appropriate safety and health practices and deter-
independently-controlled irradiation and viewing wavelengths.
mine the applicability of regulatory limitations prior to use.
The bispectrometer is designed to allow for calibration to
provide quantitative determination of the bispectral radiation-
2. Referenced Documents 5
transfer properties of the specimen (1).
2
2.1 ASTM Standards:
NOTE 1—Typically, a reference detection system monitors the radiation
E284 Terminology of Appearance
incident on the specimen. This reference detection system serves to
E308 PracticeforComputingtheColorsofObjectsbyUsing
compensate for both temporal and spectral variations in the flux incident
upon the specimen, by normalization of readings from the instrument’s
the CIE System
emission detection system.
E2153 Practice for Obtaining Bispectral Photometric Data
3.2.2 diagonal elements, n—elements of a bispectral matrix
for which irradiation and viewing wavelengths are equal.
1
This practice is under the jurisdiction of ASTM Committee E12 on Color and
Appearance and is the direct responsibility of Subcommittee E12.05 on Fluores-
cence.
3
Current edition approved July 1, 2012. Published September 2012. Originally Available from U.S. National Committee of the CIE (International Commission
approved in 2001. Last previous edition approved in 2006 as E2152 - 01 (2006). on Illumination), C/o Thomas M. Lemons, TLA-Lighting Consultants, Inc., 7 Pond
DOI: 10.1520/E2152-12. St., Salem, MA 01970, http://www.cie-usnc.org.
2 4
For referenced ASTM standards, visit the ASTM website, www.astm.org, or Available from American National Standards Institute (ANSI), 25 W. 43rd St.,
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM 4th Floor, New York, NY 10036, http://www.ansi.org.
5
Standards volume information, refer to the standard’s Document Summary page on The boldface numbers in parentheses refer to a list of references at the end of
the ASTM website. this standard.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

---------------------- Page: 1 ----------------------
E2152 − 12
3.2.3 fluorescence, n—this standard uses the term “fluores- follow the recommendations of Practice E308. For fluorescent
cence” as a general term, including both true fluorescence samples, however, special attention must be given to the
-8
(with a luminescent decay time of less than 10 s) and relative UV content of the selected illuminants and the light
...

This document is not anASTM standard and is intended only to provide the user of anASTM standard an indication of what changes have been made to the previous version. Because
it may not be technically possible to adequately depict all changes accurately, ASTM recommends that users consult prior editions as appropriate. In all cases only the current version
of the standard as published by ASTM is to be considered the official document.
Designation:E2152–01(Reapproved2006) Designation: E2152 – 12
Standard Practice for
Computing the Colors of Fluorescent Objects from
1
Bispectral Photometric Data
This standard is issued under the fixed designation E2152; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
INTRODUCTION
The fundamental procedure for evaluating the color of a fluorescent specimen is to obtain bispectral
photometric data for specified irradiating and viewing geometries, and from these data to compute
tristimulus values based on a CIE (International Commission on Illumination) standard observer and
a CIE standard illuminant. Procedures for such computation are contained in this practice. This
practice also contains procedures for computing illuminant-specific spectral radiance factor values
from illuminant-independent bispectral photometric data.
1. Scope
1.1 This practice provides the values and practical computation procedures needed to obtain tristimulus values, designated X,
Y, Z and X ,Y ,Z for the CIE 1931 and 1964 observers, respectively, from bispectral photometric data for the specimen.
10 10 10
Procedures for obtaining such bispectral photometric data are contained in Practice E2153.
1.2 Procedures for conversion of results to color spaces that are part of the CIE system, such as CIELAB and CIELUV are
contained in Practice E308.
1.3 This standard may involve hazardous materials, operations, and equipment. This standard does not purport to address all
of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate
safety and health practices and determine the applicability of regulatory limitations prior to use.
2. Referenced Documents
2
2.1 ASTM Standards:
E284 Terminology of Appearance
E308 Practice for Computing the Colors of Objects by Using the CIE System
E2153 Practice for Obtaining Bispectral Photometric Data for Evaluation of Fluorescent Color
2.2 CIE Standards:
3
CIE Publication 15.2, Colorimetry
2.3 ISO Standards:
4
ISO 11476 Paper and Board—Determination of CIE-Whiteness, C/2 Degrees
3. Terminology
3.1 Definitions—The definitions contained in Terminology E284 are applicable to this practice.
3.2 Definitions of Terms Specific to This Standard:
3.2.1 bispectrometer, n—an optical instrument equipped with a source of irradiation, two monochromators, and a detection
system, such that a specimen can be measured at independently-controlled irradiation and viewing wavelengths. The
bispectrometer is designed to allow for calibration to provide quantitative determination of the bispectral radiation-transfer
properties of the specimen.(5)
NOTE 1—Typically,areferencedetectionsystemmonitorstheradiationincidentonthespecimen.Thisreferencedetectionsystemservestocompensate
1
This practice is under the jurisdiction of ASTM Committee E12 on Color and Appearance and is the direct responsibility of Subcommittee E12.05 on Fluorescence.
Current edition approved Dec.July 1, 2006.2012. Published December 2006.September 2012. Originally approved in 2001. Last previous edition approved in 20012006
as E2152 - 01 (2006). DOI: 10.1520/E2152-01R06.10.1520/E2152-12.
2
For referencedASTM standards, visit theASTM website, www.astm.org, or contactASTM Customer Service at service@astm.org. For Annual Book of ASTM Standards
volume information, refer to the standard’s Document Summary page on the ASTM website.
3
Available from U.S. National Committee of the CIE (International Commission on Illumination), C/o Thomas M. Lemons, TLA-Lighting Consultants, Inc., 7 Pond St.,
Salem, MA 01970, http://www.cie-usnc.org.
4
Available from American National Standards Institute (ANSI), 25 W. 43rd St., 4th Floor, New York, NY 10036, http://www.ansi.org.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.
1

---------------------- Page: 1 ----------------------
E2152 – 12
for both temporal and spectral variations in the flux incident upon the specimen, by normalization of readings from the instrument’’s emission detection
system.
3.2.2 diagonal elements, n—elements of a bispectral matrix for which irradiation and viewing wavelengths are equal.
3.2.3 fluorescence, n—this standard uses the term “fluorescence” as a general term, including both true fluorescence
...

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